Macrovibrissae and microvibrissae inversion and lateralization in elephants.

Elephants are known for strongly lateralized trunk behaviors, but the mechanisms driving elephant lateralization are poorly understood. Here, we investigate features of elephant mouth organization that presumably promote lateralization. We find the lower jaw of elephants is of narrow width, but is rostrally strongly elongated even beyond the jaw bone. Elephant lip vibrissae become progressively longer rostrally. Thus, elephants have two lateral dense, short microvibrissae arrays and central, less dense long macrovibrissae. This is an inversion of the ancestral mammalian facial vibrissae pattern, where central, dense short microvibrissae are flanked by two lateral macrovibrissae arrays. Elephant microvibrissae have smaller follicles than macrovibrissae. Similar to trunk-tip vibrissae, elephant lip microvibrissae show laterally asymmetric abrasion. Observations on Asian zoo elephants indicate lateralized abrasion results from lateralized feeding. It appears that the ancestral mammalian mouth (upper and lower lips, incisors, frontal microvibrissae) is shaped by oral food apprehension. The elephant mouth organization radically changed, however, because trunk-mediated feeding replaced oral apprehension. Such elephant mouth changes include the upper lip-nose fusion to the trunk, the super-flexible elongated lower jaw, the loss of incisors, and lateral rather than frontal microvibrissae. Elephants' specialization for lateral food insertion is reflected by the reduction in the centering effects of oral food apprehension and lip vibrissae patterns.

The functional anatomy of elephant trunk whiskers.

Behavior and innervation suggest a high tactile sensitivity of elephant trunks. To clarify the tactile trunk periphery we studied whiskers with the following findings. Whisker density is high at the trunk tip and African savanna elephants have more trunk tip whiskers than Asian elephants. Adult elephants show striking lateralized whisker abrasion caused by lateralized trunk behavior. Elephant whiskers are thick and show little tapering. Whisker follicles are large, lack a ring sinus and their organization varies across the trunk. Follicles are innervated by ~90 axons from multiple nerves. Because elephants don't whisk, trunk movements determine whisker contacts. Whisker-arrays on the ventral trunk-ridge contact objects balanced on the ventral trunk. Trunk whiskers differ from the mobile, thin and tapered facial whiskers that sample peri-rostrum space symmetrically in many mammals. We suggest their distinctive features-being thick, non-tapered, lateralized and arranged in specific high-density arrays-evolved along with the manipulative capacities of the trunk.

Gendered ethnic choice effects at the transition to upper secondary education in Switzerland.

Positive ethnic choice effects, namely a higher likelihood of attending more demanding educational tracks among students of immigrant origin compared to their native peers, are observed in many countries. Immigrant optimism, and thus the striving for upward social mobility, is seen as a key mechanism for explaining ethnic choice effects. However, research on this topic often ignores gendered educational pathways and trajectories. Based on data from German-speaking Switzerland on two school-leaver cohorts, our interest is on whether ethnic choice effects are observable for both female and male students whose parents were born in the Balkans, Turkey or Portugal. In addition, we examine the extent to which aspirations contribute to explaining ethnic choice effects for both genders. To disentangle the direct effect of a migration background and the mediating effect of aspirations on educational attainment at upper secondary level, we apply the reformulated KHB method in our analyzes. Overall, our findings indicate that migrant women have made up ground on their native peers between the two school-leaving cohorts, contributing to the widening of the gender gap within the migrant group of interest. Of particular importance, however, is our finding that ethnic choice effects are observed only for men, while we do not observe any evidence of ethnic choice effects in the sample of women. Consistent with previous findings, our results show that aspirations mediate part of the ethnic choice effect. Our findings support the consideration that the room for ethnic choice effects is related to the proportion of young men and women striving for academic education, with gender differences in this regard being particularly pronounced in education systems with a high degree of vocational specificity.

Gendered intergenerational educational mobility patterns converge in the cohort sequence: evidence from Switzerland using administrative data.

In many societies, educational attainment determines social inequality in terms of life chances, and at the same time there is a strong link between social origin and educational success. Therefore, analysis of educational mobility patterns is a central concern for sociologists. In the context of societal changes, such as trend of modernization, educational expansion and significantly increased female participation in education, we use administrative data from different sources (N = 556,112) to examine the extent to which absolute and relative intergenerational educational mobility has changed in Switzerland for women and men from the 1951-1990 birth cohorts. We show that there is significantly more upward than downward mobility, while a large proportion of individuals are laterally mobile. By looking at absolute mobility patterns by cohort and gender separately, we extend previous research and show that the decreasing absolute mobility rates are due to the changing educational composition of the parental generations. Following on from previous studies, we reveal that the observed trend toward less relative social mobility continues in the youngest cohorts. It is also worth noting that, while the father's educational attainment has a higher predictive power for children's education in all cohorts, the impact of the mother's education approaches that of the father. Overall, the mobility patterns of men and women converge very strongly over the cohort sequence. Beyond these substantive points, our study demonstrates the potential of using administrative data for social stratification research.

Elephant banana peeling.

VIDEO ABSTRACT.

The effects of a special sequential mixed-mode design, and reminders, on panellists' participation in a probability-based panel study.

The sequential mixed-mode strategy has become standard practice in the survey management of longitudinal studies, in order to achieve consistently high response rates. To realise this aim in a cost-efficient way, a first mode is often an online questionnaire, towards which the target persons are pushed, and a second mode is then a telephone interview, offered to those who do not respond to the initial mode. However, the rationale for using the sequential mixed-mode plus "push-to-web" strategy to reduce the burden of choosing between survey modes, in order to maximise survey participation, could be undermined if there is an overlapping field period during which the target persons could choose between two or more offered modes. The use of reminders might be useful in solving this problem of competing risks. In the context of a multiple-panel study, this question was investigated by utilising longitudinal paradata from the fieldwork, as well as procedures of event history analysis that are adequate for the analysis of processes with competing risks. First, for a web survey as the initial mode and computer-assisted telephone interview (CATI) as the subsequent mode, it was found that the idea of a sequential mixed-mode plus "push-to-web" strategy does work even in the case of competing risks in the choice of a survey mode. Second, it was confirmed that reminders are a useful instrument for stimulating panellists to choose the running survey mode. Third, and finally, it was found that this feature of survey management is effective in countering panellists' procrastination in regard to responding to a survey.

Changes in the returns to education at entry into the labour market in West Germany.

This article studies to what extent societal processes such as educational expansion, economic modernisation and business cycles have affected the returns to educational certificates of women and men entering the labour market in West Germany. Using longitudinal data, long-term changes in cohort- and period-specific effects on socio-economic status attainment at entry into the labour market are investigated between 1945 and 2008. Analyses demonstrate that the entrants' average socio-economic prestige scores have clearly risen in the process of modernisation. Despite educational expansion, increasing skill demands for highly qualified graduates resulted in rising rates of returns for the most highly educated entrants across birth cohorts. While educational expansion and economic modernisation have boosted socio-economic returns at entry into the labour market for women from all educational levels, it has not been the case for men with the lowest levels of education. Both educational expansion and rising skill requirements of occupations led to an increasing polarisation of inequality between tertiary educated labour-market entrants and less-qualified school leavers. Educational expansion in West Germany has therefore never exceeded the occupational skill demands at entry into the labour market.

Tomato leaf curl Kunene virus: a novel tomato-infecting monopartite begomovirus from Namibia.

This is the first description of the complete genome sequence of a new monopartite begomovirus isolated from tomato with symptoms of tomato (yellow) leaf curl disease collected in northwestern Namibia, which we provisionally name "tomato leaf curl Kunene virus". The DNA-A-like nucleotide sequence shares the highest nucleotide sequence identity (82.8%) with tobacco leaf curl Zimbabwe virus (ToLCZWV). Phylogenetic analysis confirmed its affiliation with Old World monopartite begomoviruses. The discovery of a new begomovirus in a vegetable plant in Namibia highlights the importance of maintaining epidemiological surveillance in southern Africa.

Cash vs. vouchers vs. gifts in web surveys of a mature panel study--Main effects in a long-term incentives experiment across three panel waves.

In this study, we evaluate short- and long-term effects of three different prepaid incentives: a ballpoint-pen (gift worth approximately 2 Swiss francs), a voucher (cash card worth 10 Swiss francs) and cash (a 10-Swiss-francs' banknote) on young panellists' cooperation and response rate in three waves of a mature panel study with a sequential multi-mode design (web-based online survey, CATI, and PAPI). The survey experiment involved an alternative procedure to analyse the effect of different types of prepaid incentives, taking selective attrition into account as well as considering problems related to causal inference. The subjects were students, from randomly-selected school classes, who had finished their compulsory school in 2013. The findings are clear: cash provides the strongest direct, positive effect on the overall response rate and also on the latency until response after first contact. The other incentives did not work as efficiently as did cash. Additionally, cash is the most likely to minimise social selectivity in response. Finally, cash provides the potential to convert refusals in previous waves into cooperation.

Relationship between Remote Sensing Data, Plant Biomass and Soil Nitrogen Dynamics in Intensively Managed Grasslands under Controlled Conditions.

The sustainable use of grasslands in intensive farming systems aims to optimize nitrogen (N) inputs to increase crop yields and decrease harmful losses to the environment at the same time. To achieve this, simple optical sensors may provide a non-destructive, time- and cost-effective tool for estimating plant biomass in the field, considering spatial and temporal variability. However, the plant growth and related N uptake is affected by the available N in the soil, and therefore, N mineralization and N losses. These soil N dynamics and N losses are affected by the N input and environmental conditions, and cannot easily be determined non-destructively. Therefore, the question arises: whether a relationship can be depicted between N fertilizer levels, plant biomass and N dynamics as indicated by nitrous oxide (N2O) losses and inorganic N levels. We conducted a standardized greenhouse experiment to explore the potential of spectral measurements for analyzing yield response, N mineralization and N2O emissions in a permanent grassland. Ryegrass was subjected to four mineral fertilizer input levels over 100 days (four harvests) under controlled environmental conditions. The soil temperature and moisture content were automatically monitored, and the emission rates of N2O and carbon dioxide (CO2) were detected frequently. Spectral measurements of the swards were performed directly before harvesting. The normalized difference vegetation index (NDVI) and simple ratio (SR) were moderately correlated with an increasing biomass as affected by fertilization level. Furthermore, we found a non-linear response of increasing N2O emissions to elevated fertilizer levels. Moreover, inorganic N and extractable organic N levels at the end of the experiment tended to increase with the increasing N fertilizer addition. However, microbial biomass C and CO2 efflux showed no significant differences among fertilizer treatments, reflecting no substantial changes in the soil biological pool size and the extent of the C mineralization. Neither the NDVI nor SR, nor the plant biomass, were related to cumulative N2O emissions or inorganic N at harvesting. Our results verify the usefulness of optical sensors for biomass detection, and show the difficulty in linking spectral measurements of plant traits to N processes in the soil, despite that the latter affects the former.

Consanguinity and pregnancy outcomes in a multi-ethnic, metropolitan European population.

OBJECTIVE: The aim of the present study was to assess the risk of major anomalies in the offspring of consanguineous couples, including data on the prenatal situation. METHODS: Over 20 years (1993-2012), 35,391 fetuses were examined by prenatal sonography. In 675 cases (1.9%), parents were consanguineous, with 307 couples (45.5%) related as first cousins, 368 couples (54.5%) beyond first cousins. Detailed information was retrieved on 31,710 (89.6%) fetuses, (consanguineous 568: 1.8%). RESULTS: Overall prevalence of major anomalies among fetuses with non-consanguineous parents was 2.9% (consanguineous, 10.9%; first cousins, 12.4%; beyond first cousins, 6.5%). Adjusting the overall numbers for cases having been referred because of a previous index case, the prevalences were 2.8% (non-consanguineous) and 6.1% (consanguineous) (first cousin, 8.5%; beyond first cousin, 3.9%). Further adjustment for differential rates of trisomic pregnancies indicated 2.0%/5.9% congenital anomalies (non-consanguineous/consanguineous groups), that is, a consanguinity-associated excess of 3.9%, 6.1% in first cousin progeny and 1.9% beyond first cousin. CONCLUSIONS: The prevalence of major fetal anomalies associated with consanguinity is higher than in evaluations based only on postnatal life. It is important that this information is made available in genetic counselling programmes, especially in multi-ethnic and multi-religious communities, to enable couples to make informed decisions.

Individual risk assessment of adverse pregnancy outcome by multivariate regression analysis may serve as basis for drug intervention studies: retrospective analysis of 426 high-risk patients including ethical aspects.

OBJECTIVE: To identify patients at very high risk for adverse pregnancy outcome (APO) at the 20- to 23-week scan and to assess the effectiveness of Aspirin (ASS) and low molecular weight heparin (LMWH) starting after this examination. PATIENTS AND METHODS: By applying an algorithm based on multivariate logistic regression analysis using the parameters maternal age, parity, body mass index (BMI), mean pulsatility index of both uterine arteries (meanPI), presence of uni- or bilateral notch, and depth of notch (mean notch index (meanNI), we retrospectively calculated the individual risk for APO of 21,302 singleton pregnancies. We isolated a subgroup of 426 patients with the highest calculated probability for APO (cpAPO > 27.8 %). 147 had been treated with ASS; 73 with LMWH, 15 patients with a combination of ASS and LMWH, and 191 patients had not received anticoagulants. RESULTS: Administration of ASS starting after 20 gestational weeks in comparison to non-treated patients significantly reduced the frequency of intrauterine/neonatal death (IUD/NND), preeclampsia <33 weeks (PE < 33), and preterm delivery <33 weeks (PD < 33), while the frequency of IUGR showed a tendency to be elevated (P = 0.061). The subgroup of high-risk patients treated with LMWH was characterised by a higher a priori risk for APO and showed no significant reduction of any form of APO but an increased frequency of PE. CONCLUSION: Individual assessment of risk for APO by applying a simple algorithm based on biometrical/biographical as well as sonographic parameters may serve as basis for drug intervention studies. The administration of ASS in high-risk patients starting after 20 gestational weeks reduced the frequency of most of the severe forms of adverse pregnancy outcome in high-risk patients. A complication-reducing effect of LMWH starting after 20 weeks of gestation in patients could not be proven. From an ethical point of view, it may not be justified any more to preclude high-risk patients from administration of ASS or to perform studies of ASS against placebo.

Lithium modulates cancer cell growth, apoptosis, gene expression and cytokine production in HL-60 promyelocytic leukaemia cells and their drug-resistant sub-clones.

Lithium has been an FDA-approved and preferred drug for the treatment of mood disorders for many years, and cumulative evidence has pointed towards its potential use as an anti-cancer agent. Previous studies in our laboratory have demonstrated that lithium induces apoptotic cell death in HL-60 promyelocytes at concentrations of 10 mM and above. A lithium-tolerant HL-60 sub-clone, resistant to up to 15 mM lithium, was also generated and its growth profile reported. Treatment of cells with lithium resulted in a dose-dependent induction of p53, retinoblastoma (Rb) and bax expression which was accompanied by concomitant inhibition of bcl-2 expression as demonstrated using immunohistochemical microscopy. These results seem to suggest that lithium induced cell death in these cells by inhibiting expression of anti-apoptotic protein, bcl-2, while inducing higher expression of its pro-apoptotic counterparts which include bax. Expression of bax and bcl-2 is also linked to expression of inflammation-regulating cytokines. Using ELISA assays, lithium was demonstrated to induce production of pro-inflammatory cytokines, IL-6 and TNF-α, while inhibiting release of anti-inflammation-related IL-2 and IL-10 in a dose-dependent fashion. Our findings identify a critical function for lithium in modulating pro- versus anti-apoptotic gene expression and pro- versus anti-inflammatory cytokines in vitro and provide a rationale for suggesting a promising role of lithium in regulation of inflammation and cancer growth.

Noninvasive prenatal detection of chromosomal aneuploidies using different next generation sequencing strategies and algorithms.

OBJECTIVE: Here we describe the successful application of massively parallel sequencing for noninvasive prenatal detection of trisomy 21. In addition, for the detection of a broader spectrum of fetal aneuploidies, a target enrichment approach was successfully tested. METHODS: The circulating cell-free DNA was prepared from 53 maternal blood samples and analysed using Illumina's sequencing systems Genome Analyzer(IIx) and HiSeq2000, respectively. In a first experiment the SureSelect Target Enrichment System was tested. RESULTS: In our initial study analysing 42 samples on the Genome Analyzer(IIx) , all eight samples from women carrying a trisomy 21 fetus were correctly identified. On the basis of our HiSeq2000 sequence data, we discussed new algorithms for detection of fetal trisomy 21. In addition, we successfully used the combination of a target enrichment system followed by sequencing and were able to identify fetal trisomy 13 and fetal trisomy 21. CONCLUSIONS: Our results confirm previous reports that massively parallel sequencing of cell-free fetal DNA allows the reliably noninvasive detection of trisomy 21 from maternal blood with the potential to enhance test selectivity and specificity by bioinformatic means. According to our preliminary results, targeted sequencing might be an alternative strategy to detect chromosomal aneuploidies besides trisomy 21.

'Normal' nuchal translucency: a justification to refrain from detailed scan? Analysis of 6858 cases with special reference to ethical aspects.

OBJECTIVE: To assess the prevalence and detection rate of major anomalies (MAs) by applying first trimester anomaly scan (FTAS) including first trimester fetal echocardiography (FTFE) to all fetuses and discuss ethical implications. METHODS: The study group included 6879 consecutive fetuses with known outcome of pregnancy (follow-up: 98%), 6565 with 'normal' nuchal translucency (NT) (≤ P95), 314 with 'increased' NT (> P95). All fetuses received FTAS/FTFE. As MAs with the potential of being detected at FTAS/FTFE, we defined anomalies present at conception or developed during first trimester. RESULTS: Prevalence of MAs in fetuses with 'normal' NT reached 1.7%. Although 29.8% of chromosomal abnormalities were found in the group of 'normal' NT, 77% of MAs accompanied by a normal karyotype were found in this group. In fetuses with 'normal' NT and MA, diagnosis was made prenatally in 87.4% (FTAS/FTFE: 58.6%). CONCLUSION: A relevant number of MA is present in fetuses with 'normal' NT. More than half will be detected by FTAS/FTFE. As consequence, one should discuss a concept in which also in fetuses with 'normal' NT, FTAS/FTFE should be offered. This concept can also be justified from an ethical point of view, which focuses on the principles of nonmaleficence, justice and respect for autonomy of the pregnant woman.

Identification of high-risk patients for adverse pregnancy outcome based on multivariate logistic regression analysis at 20-23 gestational weeks.

AIMS: To establish and test a multivariate approach for identification of patients with high risk for adverse pregnancy outcome (APO) by a combination of sonographic (20+0-23+6 weeks) and biometrical data in a test group and to prove its effectiveness in a validation group. METHODS: In order to develop a multivariate approach for identifying patients at high risk for APO, data from 15,855 non-treated singleton pregnancies (test group A), with well-known outcomes, were analysed retrospectively. Registered parameters were parity, body mass index (BMI), mean of pulsatility index of both uterine arteries (meanPI), presence of notch, and depth of notch described as the mean of the notch index of both uterine arteries (meanNI). In a second step, the model was validated in 3678 subsequent non-treated singleton pregnancies (validation group B). RESULTS: In test group A, the calculated probabilities of APO were found between 1.3% and 82.8%, with the 95th centile at 11.2% and the 99th centile at 28.3%. In validation group B, in patients with a low calculated risk for APO <10%, complications were found in 4.4%. In patients with an intermediate risk of APO between 10% and 28%, complications were found in 10.7%. In patients from group B who were identified to be at high risk with a calculated probability of APO >28%, complications were found in 39.1%. CONCLUSION: A multivariate approach toward risk-assessment of APO seems to be a promising method for identifying patients who are at high risk for APO. Besides the individual consequence of intensive surveillance, the identification of groups of patients at high risk for APO might serve as a basis for interventional studies.

First experiences with negative pressure wound therapy and instillation in the treatment of infected orthopaedic implants: a clinical observational study.

PURPOSE: Infections associated with orthopaedic implants remain a serious complication. The main objective in acute infection control is component retention, whereas this option is usually not considered for chronic infections. METHODS: This multi-centre prospective, non-randomised observational study investigated one possible treatment option for implant retention in combination with negative pressure wound therapy with instillation (NPWTi). Thirty-two patients with an infected orthopaedic implant were analysed. Twenty-two patients had an acute infection (< 8 weeks after implantation) and ten patients had a chronic infection (> 8 weeks and < 36 weeks after implant placement). Polyhexanide was used as the instillation solution in 31 of the 32 cases. RESULTS: Nineteen patients (86.4%) with an acute infection and eight patients (80%) with a chronic infection retained their implant at 4-6 months follow-up after treatment. CONCLUSIONS: Our study showed that NPWTi can be used as adjunctive therapy for salvage of acutely infected orthopaedic implants and may even be considered for early chronically infected implants.

Doppler sonography of uterine arteries at 20-23 weeks: depth of notch gives information on probability of adverse pregnancy outcome and degree of fetal growth restriction in a low-risk population.

OBJECTIVE: Our purpose was to assess the relation between the depth of notch in the uterine arteries at 20-23 weeks and the incidence and severity of subsequent pregnancy complications. METHODS: The Notch Index (NI) was defined as (C - D)/C, with C = postsystolic zenith and D = postsystolic nadir. For evaluation, the mean NI of the two uterine arteries was determined. Adverse pregnancy outcome was defined as presence of SGA < or =5th percentile, pre-eclampsia, preterm delivery <33 weeks, placental abruption or intrauterine or neonatal death. Women who received preventive treatment (Aspirin or low-molecular heparin) were excluded. RESULTS: A notch was present in 957 of 15,888 singleton pregnancies (6.0%; unilateral = 4.37%, bilateral = 1.66%). The incidence of adverse pregnancy outcome was 5.3%; it increased from 4.6% for women without notch to 41.8% for those with a mean NI > or =0.2 and higher. Moreover, higher mean NI values tended to be associated with lower birth weight. In addition to the maternal age, parity, mean Pulsatility Index and presence of notch, the depth of notch made an additional significant contribution to the assessment of the probability of adverse pregnancy outcome. CONCLUSION: Besides the classic parameters such as maternal age, history of adverse pregnancy outcome, parity, impedance and the presence of notch, also the depth of notch seems to be valuable in the assessment of adverse pregnancy outcome risk. Especially in cases where very high or very low values of NI are measured, quantification of the depth of notch may give more information than the description of the presence of notch alone. This might be of value for future multivariate models for risk assessment of adverse pregnancy outcome.

Porcine proximal tubular cells (LLC-PK1) are able to tolerate high levels of lithium chloride in vitro: assessment of the influence of 1-20 mM LiCl on cell death and alterations in cell biology and biochemistry.

Lithium, a prophylactic drug for the treatment of bipolar disorder, is prescribed with caution due to its side effects, including renal damage. In this study porcine LLC-PK1 renal tubular cells were used to establish the direct toxicity of lithium on proximal cells and gain insights into the molecular mechanisms involved. In the presence of LiCl, cell proliferation exhibited insignificant decreases in a concentration-dependent manner, but once confluent, constant cell numbers were observed. Cell cycle studies indicated a small dose-dependent accumulation of cells in the G2/M stage after 24 h, as well as an increase in cells in the G0/G1 phase after treatment with 1-10 mM LiCl, but not at 20 mM LiCl. No evidence of apoptosis was observed based on cell morphology or DNA fragmentation studies, or evidence of protein expression changes for Bax, Bcl-2, and p53 proteins using immunocytochemistry. In addition caspases 3, 8 and 9 activity remained unaltered between control and lithium-treated cultures. To conclude, exposure to high concentrations of lithium did not result in overt toxic effects to LLC-PK1 renal cells, although LiCl did alter some aspects of cell behaviour, which could potentially influence function over time.