RESUMEN
We report results of spectral- and time-resolved study of Radachlorin photosensitizer luminescence in water in the spectral range of 950-1350nm and for determination of the photosensitizer triplet state and the singlet oxygen lifetimes responsible for singlet oxygen generation and degradation. At any wavelength within the explored spectral range the luminescence decay contained two major contributions: a fast decay at the ns time scale and a slow evolution at the µs time scale. The fast decay was attributed to electric dipole fluorescence transitions in photosensitizer molecules and the slow evolution to intercombination phosphorescence transitions in singlet oxygen and photosensitizer molecules. Relatively high-amplitude ns peak observed at all wavelengths suggests that singlet oxygen monitoring with spectral isolation methods alone, without additional temporal resolution can be controversial. In the applied experimental conditions the total phosphorescence signal at any wavelength contained a contribution from the photosensitizer triplet state decay, while at 1274nm the singlet oxygen phosphorescence dominated. The results obtained can be used for optimization of the methods of singlet oxygen monitoring and imaging.
RESUMEN
The article deals with frequency data on polymorphism of candidate genes participating in endothelial dysfunction (EDN1 Lys198Asn, NOS3 T786C, AGT Thrl74Met and AGT Met23SThr) in totality with concentrations of their active substances in individuals exposed to mercury. Findings are changes in levels of nitrogen oxide, endothelin-1, angiotensin II metabolites in examinees including those without cardiovascular diseases. The genetic conditionality is connected with unfavorable genotypes of polymorphic variants - Met235Thr of AGT gene and Lys198Asn of EDNI gene. Changes in levels of biochemical markers of endothelial dysfunction in individuals exposed to mercury indicate serious endothelial function disorders and are not genetically determined processes.
Asunto(s)
Angiotensinógeno , Endotelina-1 , Endotelio Vascular , Intoxicación por Mercurio , Mercurio , Óxido Nítrico Sintasa de Tipo III , Angiotensinógeno/genética , Angiotensinógeno/metabolismo , Enfermedades Cardiovasculares/metabolismo , Enfermedades Cardiovasculares/fisiopatología , Endotelina-1/genética , Endotelina-1/metabolismo , Endotelio Vascular/efectos de los fármacos , Endotelio Vascular/metabolismo , Endotelio Vascular/fisiopatología , Exposición a Riesgos Ambientales/efectos adversos , Femenino , Humanos , Masculino , Mercurio/química , Mercurio/toxicidad , Intoxicación por Mercurio/genética , Intoxicación por Mercurio/metabolismo , Intoxicación por Mercurio/fisiopatología , Persona de Mediana Edad , Óxido Nítrico/metabolismo , Óxido Nítrico Sintasa de Tipo III/genética , Óxido Nítrico Sintasa de Tipo III/metabolismo , Polimorfismo Genético , Estadística como AsuntoRESUMEN
The paper presents data on the frequency of polymorphisms of candidate genes involved in the formation of endothelial dysfunction--endothelin-1 (EDN1 Lys198Asn) and endothelial nitric oxide synthase (NOS3 T786C) together with the concentrations of their active products (nitric oxide, endothelin-1) in individuals with chronic mercury intoxication. The concentration change of nitric oxide and endothelin-1 indicates the presence of endothelial dysfunction in the individuals examined. The studied polymorphisms appeared to play a minor role in the pathogenesis of endothelial dysfunction in patients with chronic mercury intoxication.