RESUMEN
BACKGROUND AND OBJECTIVES: Detection of anti-platelet antibodies is required for the diagnosis of foetal/neonatal alloimmune thrombocytopaenia. The most commonly used methods for anti-platelet antibody detection are the monoclonal antibody-specific immobilization of platelet antigens (MAIPA) and the Luminex bead assay (PakLx). However, for economic reasons, the use of the PakLx assay is limited. MATERIALS AND METHODS: In the present study, we evaluated the performance of an optimized protocol based on a half-volume of PakLx reagents. We compared two alternative procedures: one with a half-volume of all components including patient samples, and another based on a half-volume of reagents but a standard volume of patient sample. RESULTS: Our results obtained with a panel of 67 samples demonstrate improved sensitivity when using a standard sample volume. CONCLUSION: In the event of an inconclusive result with this optimized protocol (e.g., incomplete panel of positive Luminex beads), we recommend testing the sample with an alternative protocol (e.g., MAIPA or the original PakLx protocol).
Asunto(s)
Antígenos de Plaqueta Humana , Recién Nacido , Humanos , Análisis Costo-Beneficio , Isoanticuerpos , Pruebas Inmunológicas , Anticuerpos Monoclonales , PlaquetasRESUMEN
DQA1*05:18 differs from DQA1*05:01:01:03 by one nucleotide substitution at position 94 in exon 2.
Asunto(s)
Secuenciación de Nucleótidos de Alto Rendimiento , Alelos , Cadenas alfa de HLA-DQ/genética , Humanos , Análisis de Secuencia de ADNRESUMEN
DQA1*03:11 differs from DQA1*03:03:01:01 by one nucleotide substitution at position 664 in exon 4.
Asunto(s)
Secuenciación de Nucleótidos de Alto Rendimiento , Alelos , Cadenas alfa de HLA-DQ/genética , Humanos , Análisis de Secuencia de ADNRESUMEN
DQA1*01:39 differs from DQA1*01:02:01:01 by one nucleotide substitution at position 49 in exon 1.
Asunto(s)
Secuenciación de Nucleótidos de Alto Rendimiento , Alelos , Cadenas alfa de HLA-DQ/genética , Humanos , Análisis de Secuencia de ADNRESUMEN
B*51:296 differs from B*51:01:01:01 by one nucleotide substitution at position 1030 in exon 6.
Asunto(s)
Antígenos HLA-B , Secuenciación de Nucleótidos de Alto Rendimiento , Alelos , Exones/genética , Antígenos HLA-B/genética , Humanos , Mutación MissenseRESUMEN
B*40:450 differs from B*40:01:02:01 by one nucleotide substitution at position 5 in exon 1.
Asunto(s)
Genes MHC Clase I , Antígenos HLA-B , Alelos , Exones/genética , Antígenos HLA-B/genética , Secuenciación de Nucleótidos de Alto Rendimiento , HumanosRESUMEN
B*44:452 differs from B*44:02:01:01 by one nucleotide substitution at position 527 in exon 3.
Asunto(s)
Genes MHC Clase I , Antígenos HLA-B , Alelos , Exones/genética , Antígenos HLA-B/genética , Secuenciación de Nucleótidos de Alto Rendimiento , HumanosRESUMEN
DQB1*03:417 differs from DQB1*03:01:01:01 by one nucleotide substitution at position 179 in exon 2.
Asunto(s)
Secuenciación de Nucleótidos de Alto Rendimiento , Alelos , Exones/genética , Cadenas beta de HLA-DQ/genética , HumanosRESUMEN
B*44:476 differs from B*44:03:01:01 by one nucleotide substitution at position 934 in exon 5.
Asunto(s)
Genes MHC Clase I , Antígenos HLA-B , Alelos , Exones/genética , Antígenos HLA-B/genética , Secuenciación de Nucleótidos de Alto Rendimiento , HumanosRESUMEN
DRB1*01:107 differs from DRB1*01:01:01:01 by one nucleotide substitution at position 484 in exon 3.
Asunto(s)
Cadenas HLA-DRB1 , Alelos , Exones/genética , Cadenas HLA-DRB1/genética , Secuenciación de Nucleótidos de Alto Rendimiento , HumanosRESUMEN
DRB1*11:260 differs from DRB1*11:03:01 by one nucleotide substitution at position 484 in exon 3.
Asunto(s)
Cadenas HLA-DRB1 , Alelos , Exones/genética , Cadenas HLA-DRB1/genética , Secuenciación de Nucleótidos de Alto Rendimiento , HumanosRESUMEN
A*24:470 differs from A*24:02:01:01 by one nucleotide substitution at position 46 in exon 1.
Asunto(s)
Secuenciación de Nucleótidos de Alto Rendimiento , Nucleótidos , Alelos , Exones/genética , Antígenos HLA-A , HumanosRESUMEN
B*15:474 differs from B*15:01:01:01 by one nucleotide substitution at position 923 in exon 5.
Asunto(s)
Genes MHC Clase I , Antígenos HLA-B , Alelos , Exones/genética , Antígenos HLA-B/genética , Secuenciación de Nucleótidos de Alto Rendimiento , HumanosRESUMEN
DQB1*03:400N differs from DQB1*03:01:01:03 by one nucleotide insertion at position 535 in exon 3.
Asunto(s)
Secuenciación de Nucleótidos de Alto Rendimiento , Alelos , Exones/genética , Cadenas beta de HLA-DQ/genética , HumanosRESUMEN
DQB1*06:352 differs from DQB1*06:03:01:01 by one nucleotide substitution at position 668 in exon 4.
Asunto(s)
Secuenciación de Nucleótidos de Alto Rendimiento , Alelos , Exones/genética , Cadenas beta de HLA-DQ/genética , HumanosRESUMEN
A*32:134 differs from A*32:01:01:01 by one nucleotide substitution at position 925 in exon 5.