The oral cavity can be explored as an area to identify the impacts of aging. Exfoliative cytology is a painless, rapid, and effective method for collecting intact cells from various epithelial layers for microscopic examination. Therefore, the current study's objective was to determine the average cell size using morphometric analysis and radiovisiography (RVG) to estimate an individual's age utilizing buccal smears and the pulp/tooth ratio. A software program was utilized to determine the cell size using exfoliative cytology, and the pulp and tooth area was measured using RVG of mandibular canine with the help of an image analysis software. Appropriate statistical tests were performed using IBM SPSS statistical software for Windows (version 25). The patient's chronological age, pulp area, and the associated pulp-tooth area ratio values were determined, along with Karl Pearson's correlation coefficient. It was discovered by using a paired t-test to compare chronological age to the age deduced from cell size and pulp-tooth area ratio. According to the current study, age estimation could be done based on average cell size, pulp size, and pulp-tooth area ratio for forensic odontology. Studies with bigger sample sizes and diverse population would help achieve definitive conclusions about the reliability of this particular method.
Probiotics, like lactobacilli and bifidobacteria, benefit health by populating the digestive system, which houses numerous microbial species. Studies demonstrate their ability to inhibit biofilm formation, crucial in preventing oral conditions like dental caries. Our research evaluated a probiotic strain's anti-biofilm efficacy against oral pathogens in 45 individuals' saliva, alongside its biofilm-forming potential. Analysis revealed significant biofilm inhibition in 36 samples. Comparisons based on age, gender, and geography further supported these findings. We propose further exploration of probiotics tailored to specific demographics to enhance oral health outcomes, suggesting a promising avenue for preventing oral microbial diseases.
Recent advancements in oral cancer detection prioritize non-invasive and minimally invasive techniques for efficient and accurate screening. This review outlines progress in methods such as narrow band imaging, fluorescence imaging, and optical coherence tomography, showing promise in early lesion detection. Biomarker detection in saliva and targeted nanoparticles enhance early diagnosis, while machine learning improves diagnostic accuracy. However, clinical validation and large-scale studies are needed for widespread adoption.
The evaluation of conventional two-dimensional radiography is crucial in making the diagnosis of periodontitis. Radio-visiography (RVG) is one of the digital imaging modalities that have received significant recognition as a replacement for film-based radiography due to its improved image quality, quick turnaround time, and minimal radiation dose. In order to compare the diagnostic efficacy of Direct Digital and Conventional Intraoral Peri-apical Radiographs in identifying Interdental Bone Loss, the present investigation was carried out. A cross-sectional observational study was conducted among 96 patients prospectively. Radiographs were exposed using a Gendex Oralix AC machine. Statistical data was analyzed using IBM SPSS statistical software using appropriate statistical tests. For both age categories, the radiographic method yielded a higher mean score than the periodontal probe method. The mean scores were noted to be higher following surgery. The radiographic approach recorded a greater mean score when gender groups were compared than the periodontal probe, which had a lower mean score. The mean scores after surgery were noted to be lower than the radiography approach and higher than the periodontal probe. The results demonstrate the radiographic technique's significant advantage over clinical tests in determining furcation regions as well as its superior accuracy in spotting periodontal disintegration over clinical probing.
Undifferentiated pleomorphic sarcoma (UPS) is a rare entity found in the oral cavity which involves the mandible in only 3% of all UPS bone lesions. The correlation of the clinical presentation of the neoplasm with the histopathological and immunohistochemistry findings is very important for appropriate diagnosis. Its incidence in membranous bone like mandible is rare. According to our literature search in the PubMed database, there are only four reported cases from India some of which does not have any immunohistochemistry findings to confirm the diagnosis. We present a rare case of UPS of mandible which was clinically mimicking squamous cell carcinoma. Upon immunohistochemical analysis, we found vimentin, KI67, CD68 positive and desmin, Melan A, LCA, S100 and myogenin negative, which lead us to the diagnosis of UPS.
Dental casting machine is an electrical device used to extrude molten materials to fabricate dental prostheses such as crowns, bridges, intracoronal and extracoronal restorations, and removable partial dentures. The casting process basically include melting and casting. Firstly, the solid material is heated in a crucible in temperature-controlled conditions to melt the material to its smelled form. The dental casting process is a complex one with multifaceted steps and equipment. Different types of casting machines are available to produce heat using different sources and techniques. It includes Arc melting, Open flame casting, and Electrical resistance. Arc melting involves the application of an electric or gas discharge on tungsten electrodes, causing the metal base to melt utilizing the heat produced by arcing. Open flame casting or induction melting employs water-cooled alternate current induction coils to induce heat. However, resistance heating uses electric current to melt precious metals.
[This corrects the article DOI: 10.7759/cureus.49536.].
Ameloblastoma is a benign yet locally aggressive tumor of the jaw bones and is most commonly found in the lower mandibular region. Histologically, it shows benign characteristics. However, ameloblastoma can turn malignant to show a more aggressive clinical course. Carcinoma ex ameloblastoma is an extremely rare malignancy arising from a pre-existing long-standing ameloblastoma or a recurrence of an ameloblastoma. According to the literature search, six to seven cases have so far been documented, and the majority of the lesions had a propensity to metastasize. Here, we present a case of carcinoma ex ameloblastoma implicating a 19-year-old male patient manifesting in the mandible, which arises from pre-existing ameloblastoma.
CONTEXT: Oral cancer is the major cause of mortality and morbidity worldwide. There are many factors that influence the tumor microenvironment that promotes tumorigenesis. Hypoxia is one of the factors that affects the process of angiogenesis by inducing proangiogenic factors to maintain the blood supply which in turn enhances the aggressiveness of the tumor and prognosis of solid tumors such as oral squamous cell carcinoma. AIM AND OBJECTIVE: The aim of the study was to compare the expression of hypoxia-inducible factor 1α (HIF-1α) and hypoxia-inducible factor 2α (HIF-2α) in various histological grades of oral squamous cell carcinoma immunohistochemically. METHODOLOGY: Immunohistochemical evaluation of HIF-1α and HIF-2α was done in 90 samples of oral squamous cell carcinoma which were graded histologically into 30 samples each of well, moderately and poorly differentiated squamous cell carcinoma. Statistical evaluation: Statistical analysis was done to study the prognostic significance of the biomarkers. RESULTS: All the cases showed positivity for expression of HIF-1α and HIF-2α. The number of positive staining in both markers reduced as the tumor severity increased from well to poorly differentiated. The expression of MIL of HIF-2α was higher than HIF 1α and HIF 2α expression was mostly seen in cytoplasmic in well-differentiated and nuclear in both moderately and poorly differentiated OSCC suggestive that HIF-2α is a more specific marker to hypoxia. CONCLUSION: Hypoxia is an essential factor that triggers other angiogenic switch and inflammatory factors which facilitates the process of tumorigenesis. This is also important for predicting the treatment outcome and prognosis of the patients. HIF-2α is a more sensitive marker that appears to be correlated and could perhaps serve as a good surrogate marker of hypoxia.
Aim: Chewable toothbrushes were compared to traditional manual toothbrushes in terms of their ability to remove plaque and their impact on salivary pH in a sample of school-aged children. Materials and Methods: The sample size for this research was 200 youngsters aged 10-12. Saliva was collected by spitting into a clean container, and the pH levels were measured using color-coded pH strips to determine where the person was starting from. The plaque score was measured using the OHI-S index after the application of the disclosing agent. The pH and plaque scores were also recalculated after brushing to see how they had changed after surgery. Results: Results indicated that both manual and chewable brushes significantly improved post-brushing pH ratings (P = 0.001), and that plaque scores decreased (P = 0.001). When compared to conventional manual toothbrushes, the chewable brush was much more successful in reducing plaque and elevating salivary pH (P = 0.001). Conclusion: As a result, parents may offer their kids a chewable toothbrush to help them maintain good oral hygiene after lunch and on lengthy car trips, when they would not have time to wash their teeth properly.
Acinic cell carcinoma (ACC), previously called acinic cell tumor, is an uncommon malignant neoplasm that tends to recur locally without proper treatment measures. This low-grade neoplasm has four histological subtypes. We hereby report a case of a follicular variant of ACC, which is a rare subtype. A 20-year literature search encompassing this variant of ACC of the parotid region was also performed, which showed four reported cases. A 60-year-old woman reported to our college, Kalinga Institute of Dental Sciences, with the chief complaint of pain and swelling in her upper right back tooth region for the past three months. Upon incisional biopsy, histopathological examination revealed a follicular variant of ACC. Further, immunohistochemistry was also performed using markers such as DOG-1, CK7, S-100, and thyroglobulin, which showed CK7 marker positivity. Subtotal parotidectomy was performed and the tissue was sent for histopathological analysis. Although ACCs are slow-growing and indolent in character, they can frequently recur locally decades later and spread to distant organs. Long-term follow-up is necessary following therapy as ACC has a noticeably high propensity to relapse and create latent metastases.
Introduction: India is one of the largest countries which is inhabited by diverse populations of different tribes, castes, religious, and migrant groups. In forensic investigation, personal identification plays the greatest importance. Lip prints are considered as one such tool for personal identification. Study of lip prints is referred to as cheiloscopy and they are considered as unique in every individual just like fingerprints. Materials and Methods: Three hundred randomly selected specimens were taken in our study comprising of both the genders and of various regions from Odisha, West Bengal (WB) and Bihar was considered in the study. The age selected in our study ranged from 18 to 25 years comprising of 151 males and 149 females. Lip impression was made and viewed for different lip prints pattern under a magnifying glass of 10X. Results: There was a total of six lip print type identified in the present study. The type of lip print pattern found more in males was Type 2 and in females was Type 1. The type of lip print pattern found more in Bihar and WB was Type 2 and Odisha was Type 1. Conclusion: As the lip print is unique for every individual, Cheiloscopy is considered as a useful method of identification in forensic odontology. The study showed statistically significant difference in gender with a variation in geographical location.
Peripheral giant-cell granulomas (PGCGs) are reddish color lesions of the gingiva which are frequently diagnosed on a routine basis by many dentists in the regular dental practice. Most of the lesions of PGCG are slow-growing, nonaggressive with asymptomatic, and tend to recur. Here, we exhibit a case of PGCG of the upper jaw in a 15-year-old girl child. She presented with a chief complaint of a lump on her upper anterior tooth region for 2-3 months. A well-defined painless, sessile exophytic growth, measuring about 1 cm × 0.5 cm in dimension with pinkish red in color with a firm consistency, was noted. Moreover, no secondary changes were seen. On the radiograph also, no changes in the bone/tooth were observed. The provisional diagnosis was identified as benign lesions such as pyogenic granuloma, peripheral ossifying fibroma, and PGCG and was made based on the clinical history and radiographic findings, and the confirmative diagnosis was given after histopathological examination. To improve the esthetic and function of teeth as well as psychological problems in the young patient, we tend to manage the case more simply by conservative surgical procedure at the earliest to prevent a recurrence.
Dental caries is considered to be the most common pathology of the oral cavity. The pathogenesis of dental carries is believed to be because of imbalance in the microbiological niche creating an ecological pressure. Probiotics are living microorganisms which when dispensed in appropriate amounts can benefit the health of the host. They have posed as a promising replacement to antibiotics that can aid in solving dental issues like dental caries. There are numerous Randomized control trials to validate this fact. There are also various modes through which a probiotic can be delivered against dental caries. Numerous new avenues like effector strains and synbiotics are also explored nowadays. The present review is about the significance of probiotics in the management of dental caries.
Background: The current investigation was intended to evaluate the precision of impression cytology and tissue histology in the detection of ocular surface neoplasia. Materials and Methods: We examined the histories of patients detected with ocular surface neoplasia at the Department of Ophthalmology, Kalinga Institute of Medical Sciences, Bhubaneswar. All the patients had their impressions cytology and tissue samples taken. The outcomes of both strategies were compared. Results: There were a total of 55 patients (33 males and 22 females) aged 12-99 years (average age, 51 years). Twenty patients had injuries to the right eye and 35 had injuries to the left eye. Conjunctival-corneal intraepithelial neoplasia (38.2%), squamous cell carcinoma (34.5%), squamous epithelium (10.9%), squamous papilloma (7.3%), conjunctival nevi (7.3%), Along with the most common histological research, malignant melanoma (1.8%). Compared with the histological results, impression cytology showed positive and negative assessment accuracies of 97.4% and 52.9%, respectively. In squamous neoplasms, squamous cell carcinoma exhibited a strong link with histology. The occurrence of dysplastic cells raised suspicion of malignancy. Conclusion: Because of its increased accuracy in detecting lesions, along with tissue histology, impression cytology exhibits enormous potential in identifying ocular surface neoplasia. However, impression cytology has an undesirable prognostic accuracy, indicating that it is a useful screening tool but cannot be considered as a "gold standard."
BACKGROUND: Oral submucous fibrosis (OSF), widespread in the Indian subcontinent, is a chronic debilitating disease of the oral cavity having a high potential for malignancy. The etiology of OSF is debatable. However, recently, autoimmunity had been suggested to play a significant role in its etiology, yet unproven. Hence, this study was conducted to explore the presence of antinuclear antibodies (ANAs) in the serum of OSF patients which is one of the serum markers of autoimmune diseases and its possible clinicopathologic associations. MATERIALS AND METHODS: A total of 105 blood samples were collected from patients with OSF (n = 45), age- and sex-matched healthy controls with (n = 30) and without (n = 30) areca nut chewing habit. Serum positivity of ANA was determined by immunofluorescence and correlated with the oral habits and severity of the disease measured by maximum mouth opening (MMO) and site of involvement. RESULTS: Significantly higher incidence of ANA (35.6%) was found in 45 OSF patients than in the healthy group (P = 0.001). Prevalence of ANA positivity was found higher in females (n = 11; 68%) than males (P < 0.001). A significantly lower MMO (P = 0.00) was found in ANA positive patients (17 ± 6.21 mm) in contrast to MMO in ANA-negative patients (28.74 ± 6.58 mm). The mean duration of habit and frequency of habit between ANA positive and negative patients was not significant. A significantly more number of sites of involvement was seen in ANA positive cases (P = 0.004). Out of 16 ANA positive OSF cases, 10 cases showed + 2 and 6 cases showed + 3 fluorescence intensity. Speckled (n = 8), homogeneous (n = 5) and nucleolar pattern (n = 3) were the fluorescence patterns observed. CONCLUSION: The presence of autoantibodies such as ANA, female predilection, alteration of humoral and cellular immunity justifies OSF as an autoimmune disease. This study provides broader prospective to adopt therapies that selectively target autoimmune pathways.
BACKGROUND: E-Cadherin/ß-Catenin protein complexes play a major role in epithelial to mesenchymal transition (EMT) and vice versa. Such types of EMT are implicated physiologically during embryonic development and pathologically in tissue fibrosis and tumorigenesis. AIMS: The aim was the evaluation of E-Cadherin and ß-Catenin immunoreactivity in various grades of oral squamous cell carcinoma (OSCC) and to correlate their pattern of expression. MATERIALS AND METHODS: Immunohistochemical expression of E-Cadherin/ß-Catenin was evaluated in a total n = 30 tissue samples comprising of n = 10 well-differentiated squamous cell carcinoma (WDSCC), n = 10 moderately differentiated squamous cell carcinoma (MDSCC), and n = 10 poorly differentiated squamous cell carcinoma (PDSCC). Based on the intensity of staining, an immunoreactivity scoring was calculated. STATISTICAL ANALYSIS: The scorings obtained were subjected to independent t-test, paired t-test, Chi-square test, and ANOVA test using SPSS version 20.0 statistical analysis software. P < 0.05 was considered statistically significant. RESULTS: A significant difference was observed in the expression of ß-Catenin between normal mucosa and WDSCC; normal mucosa and MDSCC. A gradual decrease in the immunoreactivity score of E-Cadherin is seen in WDSCC, MDSCC, and PDSCC. CONCLUSION: Therefore, dysregulation of these proteins can lead to tumor progression, invasion, and metastasis. Further studies are warranted to specify the role of these EMT proteins as prognostic/therapeutic markers in patients suffering from OSCC.
BACKGROUND: In developing countries like India, irrespective of the various government laws and actions, substance abuse is a growing problem. Individuals with illicit drug dependence face social and psychological stigma with considerable health problems. Negligence to oral hygiene and lack of oral health treatments intensifies the sensations experienced by the subjects. AIM: The aim is to determine the patterns of substance abuse and oral health sensations among individuals with illicit drug dependence. MATERIALS AND METHODS: A cross-sectional study was conducted wherein 213 male individuals with illicit drug dependence history, admitted in rehabilitation centers of Bhubaneswar, formed the study population. Information pertaining to the type and duration of drug dependence along with oral sensations experienced by the individuals during a period of 3 months was obtained by interviewing by the subjects. Chi-square test was done to identify variations in oral sensations produced by different illicit drugs and its relationship with their usage pattern. RESULTS: About 40.8% of dependents reported cannabis intake, thus making it the most commonly consumed drug. About 80.8% of all the subjects reported dryness of the mouth, 47.9% reported sensitivity of teeth and 28.2% suffered from the numbness of their mouth. About 19.7% of the individuals felt like chewing on something, 23% inclined toward grinding, whereas 11.7% experienced pain in the jaw muscles and joint. Sixty percent of substance dependents who smoked drugs had sensitive teeth (P ≤ 0.05). All the oral sensations experienced were significantly more among drug dependents with a history of illicit drug usage for >5years in comparison to others. CONCLUSION: Substance abuse results in a range of oral sensations. De-addiction programs in India lack oral health-care components. Studies on oral complications related to addiction stresses on the need of oral healthcare at various levels, including education, prevention, and treatment. All these components should be amalgamated into general care services for addicts.
INTRODUCTION: Conventional tissue processing takes a lot of time, but microwave processing method though rapid, its quality of tissue texture and staining properties is still questionable. Therefore, we conducted this study to find out the reliability of histological sections in the microwave method. AIM AND OBJECTIVES: The aim is to assess whether microwave tissue processing, which is a rapid technique, is comparable to conventional tissue processing in terms of quality and reliability of histological sections. MATERIALS AND METHODS: A total of 80 formalin-fixed tissue samples were taken and were divided into two pieces. One subjected to conventional tissue processing and the other subjected to microwave tissue processing. The slides were coded and evaluated by three independent observers using various parameters. All the data were subjected to Chi-square test. RESULTS: Both the process did not differ in their efficiency in overall nuclear details, overall cytoplasmic detail, occurrence of artifact and tissue architecture, nuclear staining, cytoplasmic staining, and red blood cell staining. Although the other observers did not find any statistical significance, according to observer 3, conventional processing proved to have marginally significantly better epithelial connective tissue interface than microwave processing (P = 0.047). CONCLUSION: The microwave method of tissue processing is an economical procedure which reduces the obnoxious chemicals as well as the turnaround time.
BACKGROUND: Odontogenic tumors, derived from epithelial, ectomesenchymal, and/or mesenchymal elements of the tooth-forming apparatus, constitute a heterogeneous group of lesions, including hamartomas, benign and malignant neoplasms with metastatic capabilities. HIGHLIGHT: This review provides a comprehensive overviewof the pathogenesis of odontogenic tumors and explains the associated molecular events in the context of hallmarks of cancer established by Hanahan D and Weinberg RA. Diagrammatic representations depicted in the article would facilitate easier understanding. CONCLUSION: A better understanding of the pathogenesis of the lesions may assist in determining patient's prognosis and devising better targeted therapeutic treatment, thus, reducing the morbidity and mortalityof patients.
Ameloblastoma , Hamartoma , Odontogenic Tumors , Humans , Odontogenesis , Odontogenic Tumors/genetics , Prognosis
