Computational Portable Microscopes for Point-of-Care-Test and Tele-Diagnosis.

In bio-medical mobile workstations, e.g., the prevention of epidemic viruses/bacteria, outdoor field medical treatment and bio-chemical pollution monitoring, the conventional bench-top microscopic imaging equipment is limited. The comprehensive multi-mode (bright/dark field imaging, fluorescence excitation imaging, polarized light imaging, and differential interference microscopy imaging, etc.) biomedical microscopy imaging systems are generally large in size and expensive. They also require professional operation, which means high labor-cost, money-cost and time-cost. These characteristics prevent them from being applied in bio-medical mobile workstations. The bio-medical mobile workstations need microscopy systems which are inexpensive and able to handle fast, timely and large-scale deployment. The development of lightweight, low-cost and portable microscopic imaging devices can meet these demands. Presently, for the increasing needs of point-of-care-test and tele-diagnosis, high-performance computational portable microscopes are widely developed. Bluetooth modules, WLAN modules and 3G/4G/5G modules generally feature very small sizes and low prices. And industrial imaging lens, microscopy objective lens, and CMOS/CCD photoelectric image sensors are also available in small sizes and at low prices. Here we review and discuss these typical computational, portable and low-cost microscopes by refined specifications and schematics, from the aspect of optics, electronic, algorithms principle and typical bio-medical applications.

Efficient taper optical hydrogel fiber coupler drawn from suspended photocuring 3D printing.

Integrating bio-friendly optical hydrogel fibers (HFs) with solid-state fibers (SFs) could expand the horizons of fiber-optic technology for bio-photonics. However, methods for coupling HF and SF-based systems are inefficient due to the mode field mismatch. Here, a hydrogel fiber coupler with a taper core-cladding structure is demonstrated for efficiently coupling HF to SF and fabricated through suspended photocuring 3D printing. Coupling efficiencies of 8.3 and 9.4 dB are obtained at 632 and 473 nm, respectively, which are 22% better than those of conventional couplers. The working bandwidth covers visible wavelengths, satisfying bioengineering requirements. This research removes obstacles to optical fiber applications in bioscience.

Accurate color imaging of pathology slides using holography and absorbance spectrum estimation of histochemical stains.

Holographic microscopy presents challenges for color reproduction due to the usage of narrow-band illumination sources, which especially impacts the imaging of stained pathology slides for clinical diagnoses. Here, an accurate color holographic microscopy framework using absorbance spectrum estimation is presented. This method uses multispectral holographic images acquired and reconstructed at a small number (e.g., three to six) of wavelengths, estimates the absorbance spectrum of the sample, and projects it onto a color tristimulus. Using this method, the wavelength selection is optimized to holographically image 25 pathology slide samples with different tissue and stain combinations to significantly reduce color errors in the final reconstructed images. The results can be used as a practical guide for various imaging applications and, in particular, to correct color distortions in holographic imaging of pathology samples spanning different dyes and tissue types.

Optical refractometry using lensless holography and autofocusing.

Conventional optical refractometry methods are often limited by a narrow measurement range, complex hardware, or relatively high cost. Here, we present a novel refractometry method to measure the bulk refractive index (RI) of materials (including solids and liquids) using lensless holographic on-chip imaging and autofocusing, which is simple, cost-effective, and has a large RI measurement range. As a proof of concept, two compact prototypes were built to measure the RIs of solid materials and liquids, respectively, and they were tested by measuring the RIs of a ZnSe plate and a microscopy immersion oil. Experimental results show that our devices have an average accuracy of ~3 × 10-4 RI unit (RIU) with an estimated precision of ~3 × 10-3 RIU for solids; and an average accuracy of ~1 × 10-4 RIU with an estimated precision of ~3 × 10-4 RIU for liquids. We believe that this cost-effective and portable RI measurement platform holds promise to be used in laboratory and industrial settings.

Method to design two aspheric surfaces for a wide field of view imaging system with low distortion.

This paper presents a distortion correction method for designing a wide field of view (FOV) lens for an imaging system. The lens is composed of two aspheric surfaces and several spheres. In the preliminary design, profiles of the aspheric surfaces can be obtained according to aplanatism, refraction law, and polynomial fitting methods, where the numeric computation, the differential geometry computation, and the polynomial fitting algorithm are stated in detail. Then the lens is optimized by the damped least squares method. Theoretically, this method cannot eliminate aberrations absolutely but can balance some kinds of aberrations to the image well. Furthermore, a projector lens with a wide FOV, low distortion, and low throw ratio [TR = (projection distance)/(image diagonal size)] is designed successfully by this method.