Functional properties of mesenchymal stem cells labeled with magnetic microparticles in vitro and analysis of their distribution after transplantation.

Mesenchymal stem cells enzymatically isolated from human placenta were labeled with magnetic fluorescent microparticles (d=0.96 µ). We showed that microparticles in high doses (>10 µl stock suspension per 1 ml culture medium) significantly inhibited cell proliferation in culture. In our work we determined the optimal concentration of particles not affecting physiological properties of mesenchymal stem cells: it does not change cell proliferation, does not induce apoptosis, and does not modulate their transdifferentiation into neuronal cells. In vivo experiments showed that the chosen particles allow easy visualization of transplanted cells ex vivo on sections of different tissues.

[Biomedical aspects of health preservation strategy: role of catecholaminergic neuronal populations]. / Biomeditsinskie aspekty strategii sokhraneniia zdorov'ia: rol' katekholaminergicheskikh neironal'nykh populiatsii.

Lifestyle, environmental factors, genetics, and medical care are the main factors that determine the health status of man. Of particular attention are biological mechanisms ensuring the body's adaptation to constantly changing environmental conditions. The noradrenergic neuronal populations, the sympathetic nervous system in particular, modulate metabolic processes and supports a variety of activities, making them relevant to changing living conditions. There is a clear correlation between the life span and the number of sympathetic nerve cells functioning during postnatal ontogenesis. The exposures that reduce the activity of peripheral and central noradrenergic neurons and slow down aging processes in them loosen the relationships between the inner and outer world to prevent hyperactivity and to prolong life.

[Transcription of nucleoplasmatic and nucleolar sites of DNA sensitive to the protease inhibitor antipain]. / Transkriptsiia nukleoplazmaticheskikh i iadryshkovykh saitov DNK, chuvstvetel'naia k ingibitoru proteaz antipainu.

Age-related changes in the transcriptional activities of Purkinje cells chromatin and sympathetic cranial cervical ganglion under the influence of morphine and some natural and synthetic peptide compounds were found. It was shown that at different stages of the ontogenesis, morphine was capable of increasing the template activity of nucleolar and/or extranucleolar chromatin, and that this process was inhibited by antipain. The findings suggest the presence of DNA sites in the eucaryotic genome, that are induced by the protease mechanism and are likely to be similar to the SOS-genes of procaryotes. The SOS-genes of procaryotes are activated under unfavourable conditions and promote the survival of the microbial population, while in eucaryotes these genes are believed to provide the formation of definite cell populations whose composition is the most adequate for the living conditions of an adult organism.