RESUMEN
Mechanical forces are key regulators of cellular behavior and function, affecting many fundamental biological processes such as cell migration, embryogenesis, immunological responses, and pathological states. Specialized force sensors and imaging techniques have been developed to quantify these otherwise invisible forces in single cells and in vivo. However, current techniques rely heavily on high-resolution microscopy and do not allow interrogation of optically dense tissue, reducing their application to 2D cell cultures and highly transparent biological tissue. Here, we introduce DEFORM, deformable microlaser force sensing, a spectroscopic technique that detects sub-nanonewton forces with unprecedented spatio-temporal resolution. DEFORM is based on the spectral analysis of laser emission from dye-doped oil microdroplets and uses the force-induced lifting of laser mode degeneracy in these droplets to detect nanometer deformations. Following validation by atomic force microscopy and development of a model that links changes in laser spectrum to applied force, DEFORM is used to measure forces in 3D and at depths of hundreds of microns within tumor spheroids and late-stage Drosophila larva. We furthermore show continuous force sensing with single-cell spatial and millisecond temporal resolution, thus paving the way for non-invasive studies of biomechanical forces in advanced stages of embryogenesis, tissue remodeling, and tumor invasion.
RESUMEN
Compact wireless light sources are a fundamental building block for applications ranging from wireless displays to optical implants. However, their realization remains challenging because of constraints in miniaturization and the integration of power harvesting and light-emission technologies. Here, we introduce a strategy for a compact wirelessly powered light-source that consists of a magnetoelectric transducer serving as power source and substrate and an antiparallel pair of custom-designed organic light-emitting diodes. The devices operate at low-frequency ac magnetic fields (~100 kHz), which has the added benefit of allowing operation multiple centimeters deep inside watery environments. By tuning the device resonance frequency, it is possible to separately address multiple devices, e.g., to produce light of distinct colors, to address individual display pixels, or for clustered operation. By simultaneously offering small size, individual addressing, and compatibility with challenging environments, our devices pave the way for a multitude of applications in wireless displays, deep tissue treatment, sensing, and imaging.
RESUMEN
Important dynamic processes in mechanobiology remain elusive due to a lack of tools to image the small cellular forces at play with sufficient speed and throughput. Here, we introduce a fast, interference-based force imaging method that uses the illumination of an elastic deformable microcavity with two rapidly alternating wavelengths to map forces. We show real-time acquisition and processing of data, obtain images of mechanical activity while scanning across a cell culture, and investigate sub-second fluctuations of the piconewton forces exerted by macrophage podosomes. We also demonstrate force imaging of beating neonatal cardiomyocytes at 100 fps which reveals mechanical aspects of spontaneous oscillatory contraction waves in between the main contraction cycles. These examples illustrate the wider potential of our technique for monitoring cellular forces with high throughput and excellent temporal resolution.
