RESUMEN
The taxonomic position of a novel actinobacterium, strain 169T, isolated from a sample of Algerian Saharan soil, was determined using a polyphasic taxonomic approach. The aerial mycelium produced non-motile, round- to oval-shaped spores, with a smooth surface, which were sessile or carried by short sporophores. Chemotaxonomically, isolate 169T showed the same results as members of the genus Streptosporangium, but madurose, the so far diagnostic sugar of the genus, was not detected in the whole-cell hydrolysate. Despite the absence of sporangia, the 16S rRNA gene sequence analysis confirmed that strain 169T was a member of the genus Streptosporangium. Strain 169T was most closely related to Streptosporangium jomthongense NBRC 110047T (99.3 % 16S rRNA gene sequence similarity), which is the only non-sporangia-forming species reported among the genus. However, DNA-DNA hybridization studies with this species showed 60 % relatedness. Based upon genotypic and phenotypic data, a novel species, Streptosporangium algeriense sp. nov., is proposed, with 169T ( = DSM 45455T = MTCC 11561T = CCUG 62974T) as the type strain.
RESUMEN
Actinomycete strain SA198(T), isolated from a Saharan soil sample of Algeria, was characterized taxonomically by using a polyphasic approach. Chemotaxonomic and morphological characteristics observed suggested that it was a member of the genus Saccharothrix . The 16S rRNA gene sequence analysis confirmed that strain SA198(T) was a member of the genus Saccharothrix and showed a similarity level ranging between 97.5 and 98.9% within species of the genus Saccharothrix , Saccharothrix australiensis being the most closely related. However, DNA-DNA hybridization values between strain SA198(T) and its closest phylogenetic neighbours, the type strains of S. australiensis , Saccharothrix xinjiangensis , Saccharothrix algeriensis and Saccharothrix espanaensis , were clearly below the 70% threshold. Based upon genotypic and phenotypic differences from other members of the genus, a novel species, Saccharothrix tamanrassetensis sp. nov., is proposed, with SA198(T) (â=âDSM 45947(T)â=âCECT 8640(T)) as the type strain.
Asunto(s)
Actinobacteria/clasificación , Filogenia , Microbiología del Suelo , Actinobacteria/genética , Actinobacteria/aislamiento & purificación , África del Norte , Argelia , Técnicas de Tipificación Bacteriana , ADN Bacteriano/genética , Ácidos Grasos/química , Datos de Secuencia Molecular , Hibridación de Ácido Nucleico , ARN Ribosómico 16S/genética , Análisis de Secuencia de ADN , Vitamina K 2/análogos & derivados , Vitamina K 2/químicaRESUMEN
The taxonomic position of a novel actinomycete, strain SA152(T), isolated from a sample of Algerian Saharan soil, was determined using a polyphasic taxonomic approach. The strain produced abundant aerial mycelium and fragmented substrate mycelium on most media tested. Chemotaxonomically and phylogenetically, the strain was related to the members of the genus Saccharothrix. Results of 16S rRNA gene sequence comparison revealed that strain SA152(T) shared the highest degree of 16S rRNA gene sequence similarity with Saccharothrix xinjiangensis NBRC 101911(T) (99.3â%) and Saccharothrix texasensis NRRL B-16134(T) (98.9â%). However, DNA-DNA hybridization studies showed only 16.2â% relatedness with S. xinjiangensis DSM 44896(T) and 33.9â% relatedness with S. texasensis DSM 44231(T). Based upon genotypic and phenotypic differences from other members of the genus, a novel species, Saccharothrix saharensis sp. nov., is proposed, with SA152(T) (â=âDSM 45456(T)â=âCCUG 60213(T)) as the type strain.
Asunto(s)
Actinomycetales/clasificación , Filogenia , Microbiología del Suelo , Actinomycetales/genética , Actinomycetales/aislamiento & purificación , Argelia , Técnicas de Tipificación Bacteriana , ADN Bacteriano/genética , Ácido Diaminopimélico/análisis , Ácidos Grasos/análisis , Genotipo , Datos de Secuencia Molecular , Hibridación de Ácido Nucleico , Fenotipo , Fosfatidiletanolaminas/análisis , ARN Ribosómico 16S/genética , Análisis de Secuencia de ADN , Vitamina K 2/análogos & derivados , Vitamina K 2/análisisRESUMEN
An actinomycete, designated SA181(T), was isolated from Saharan soil in the Hoggar region (south Algeria) and was characterized taxonomically by using a polyphasic approach. The morphological and chemotaxonomic characteristics of the isolate were consistent with the genus Saccharothrix, and 16S rRNA gene sequence analysis confirmed that strain SA181(T) was a novel member of the genus Saccharothrix. DNA-DNA hybridization values between strain SA181(T) and its closest phylogenetic neighbours, the type strains of Saccharothrix longispora, Saccharothrix texasensis and Saccharothrix xinjiangensis, were clearly below the 70â% threshold. The genotypic and phenotypic data showed that the isolate represents a novel species of the genus Saccharothrix, for which the name Saccharothrix hoggarensis sp. nov. is proposed, with the type strain SA181(T) (â=âDSM 45457(T) â=âCCUG 60214(T)).
