New Species of Grotea (Hymenoptera, Ichneumonidae, Labeninae) from Ecuador, with New Records and a Key to the Neotropical Species.

Here we describe two new Grotea species from Ecuador, G. akakana Mazón & Bordera sp. nov., and G. romeri Mazón sp. nov., as well as the male of G. cundinamarquesa Herrera-Flórez 2018. G. akakana sp. nov. is characterized by the combination of a postgenal process long, a 45-flagellomeres antenna without a white band and a mesopleuron black with two yellow spots separated by a red one. On the other hand, G. romeri sp. nov. is characterized by the combination of a postgenal process very short, a 36-flagellomeres antenna without a white band, a propodeum with a long and narrow area lateralis, uninterrupted yellow-colored orbits and a mesopleuron black with a yellow spot in the middle. The species G. santandereana Herrera-Flórez 2018 and G. surinamese Herrera-Flórez 2019 are recorded from Ecuador for the first time. This brings the total of described Grotea species to 31, all from the New World, with 27 of these exclusively Neotropical. A key for the identification of Neotropical species is included.

Species richness estimation of the Afrotropical Darwin wasps (Hymenoptera, Ichneumonidae).

Species richness is one of the fundamental metrics of biodiversity. Estimating species richness helps spotlight taxonomic groups that are particularly under-studied, such as the highly diverse Darwin wasps. The only available estimate of the number of Darwin wasps in the Afrotropics proposed almost 11,000 species, compared to the 2,322 recorded species. However, it relied exclusively on the ratio of morphospecies to described species in Henry Townes' personal collection. We provide an updated estimate of the Afrotropical Darwin wasp species, using empirical data from multiple sources, including the increase in species numbers following generic revisions, morphospecies sorting in natural history collections, and diversity patterns of better-studied insects (butterflies) for extrapolation. Our analyses suggest that our knowledge of Darwin wasps is highly incomplete, with only 13-22% of species known in the five most extensively studied countries in the Afrotropics. We estimate 9,206-15,577 species of Darwin wasps within the entire Afrotropics, with the highest concentration expected in the Equatorial Afrotropics and Madagascar. Due to data constraints, our approach tends to underestimate diversity at each step, rendering the upper estimate (15,577 species) more realistic. We highlight reasons contributing to the gap between recorded and estimated species richness, including logistical and financial factors, as well as post-colonial influences.

The ectoparasitoid wasp Heterospilussicanus (Marshall, 1888) (Hymenoptera, Braconidae, Doryctinae) as a natural enemy of Gastralluspubens Fairmaire, 1875 (Coleoptera, Ptinidae) in Italy.

Heterospilussicanus (Marshall, 1888) is redescribed and illustrated based on the holotype of Dendrosotersicanus Marshall, 1888 and on recently collected material from its type locality (Sicily, Italy). Previous host records for this species are unreliable. Here, the host of H.sicanus, the rare ptinid beetle Gastralluspubens Fairmaire, 1875, is recorded for the first time, having been reared in a historic library in Palermo, Italy. Heterospilussicanus is compared with the similar species Telebolus (= Heterospilus) corsicus Marshall, 1888, which was described in the same monograph from Corsica (France), and it is also redescribed and illustrated. Atoreuteusceballosi Docavo Alberti, 1960, syn. nov. is synonymised under Heterospilussicanus (Marshall, 1888), and Hormiopterus (= Rhaconotus) ollivieri Giraud var. flava Fahringer, 1931, syn. nov. is a junior synonym of Heterospiluscephi Rohwer, 1925. A key for determination of the Western Palaearctic Heterospilus species with a striate vertex is provided and the distributions of H.sicanus and H.corsicus are discussed.

The genome sequence of an ichneumonid wasp, Heteropelma amictum (Fabricius, 1775).

We present a genome assembly from an individual female Heteropelma amictum (an ichneumonid wasp; Arthropoda; Insecta; Hymenoptera; Ichneumonidae). The genome sequence is 226.4 megabases in span. Most of the assembly is scaffolded into 10 chromosomal pseudomolecules. The mitochondrial genome has also been assembled and is 20.65 kilobases in length.

The genome sequence of an ichneumon wasp, Ophion slaviceki (Kriechbaumer, 1892).

We present a genome assembly from an individual female Ophion slaviceki (an ichneumon wasp; Arthropoda; Insecta; Hymenoptera; Ichneumonidae). The genome sequence is 654.2 megabases in span. Most of the assembly is scaffolded into 13 chromosomal pseudomolecules. The mitochondrial genome has also been assembled and is 16.19 kilobases in length. Gene annotation of this assembly on Ensembl identified 19,399 protein coding genes.

The genome sequence of the Black Spongefly, Sisyra nigra (Retzius, 1783).

We present a genome assembly from an individual female Sisyra nigra (the Black Spongefly; Arthropoda; Insecta; Neuroptera; Sisyridae). The genome sequence is 372.6 megabases in span. Most of the assembly is scaffolded into 7 chromosomal pseudomolecules, including the X sex chromosome. The mitochondrial genome has also been assembled and is 16.34 kilobases in length.

The genome sequence of the Cloaked Minor, Mesoligia furuncula (Denis & Schiffermüller, 1775).

We present a genome assembly from an individual female Mesoligia furuncula (the Cloaked Minor; Arthropoda; Insecta; Lepidoptera; Noctuidae). The genome sequence is 889.6 megabases in span. Most of the assembly is scaffolded into 31 chromosomal pseudomolecules, including the Z sex chromosome. The mitochondrial genome has also been assembled and is 15.3 kilobases in length. Gene annotation of this assembly on Ensembl identified 21,903 protein coding genes.

The genome sequence of the Shuttle-shaped Dart, Agrotis puta (Hübner, 1803).

We present a genome assembly from an individual male Agrotis puta (the Shuttle-shaped Dart; Arthropoda; Insecta; Lepidoptera; Noctuidae). The genome sequence is 522 megabases in span. Most of the assembly is scaffolded into 31 chromosomal pseudomolecules, including the assembled Z chromosome. The mitochondrial genome has also been assembled and is 15.4 kilobases in length. Gene annotation of this assembly on Ensembl has identified 15,136 protein coding genes.

The genome sequence of the Turnip Sawfly, Athalia rosae (Linnaeus, 1758).

We present a genome assembly from an individual female Athalia rosae (the Turnip Sawfly; Arhropoda; Insecta; Hymenoptera; Athaliidae). The genome sequence is 172 megabases in span. Most of the assembly is scaffolded into eight chromosomal pseudomolecules. The mitochondrial genome has also been assembled and is 16.3 kilobases in length. Gene annotation of this assembly on Ensembl identified 11,393 protein coding genes.

The genome sequence of an ichneumon wasp, Clistopyga incitator (Fabricius, 1793).

We present a genome assembly from an individual female Clistopyga incitator (an ichneumon wasp; Arthropoda; Insecta; Hymenoptera; Ichneumonidae). The genome sequence is 260.7 megabases in span. Most of the assembly is scaffolded into 21 chromosomal pseudomolecules. The mitochondrial genome has also been assembled and is 25.0 kilobases in length.

The genome sequence of an ichneumonid wasp, Campoletis raptor (Zetterstedt, 1838).

We present a genome assembly from an individual female Campoletis raptor (an ichneumonid wasp; Arthropoda; Insecta; Hymenoptera; Ichneumonidae). The genome sequence is 218.6 megabases in span. Most of the assembly is scaffolded into 11 chromosomal pseudomolecules. The mitochondrial genome has also been assembled and is 28.53 kilobases in length.

The genome sequence of an ichneumonid wasp, Tromatobia lineatoria (Villers, 1789).

We present a genome assembly from an individual female Tromatobia lineatoria (an ichneumonid wasp; Arthropoda; Insecta; Hymenoptera; Ichneumonidae). The genome sequence is 383.6 megabases in span. Most of the assembly is scaffolded into 21 chromosomal pseudomolecules. The mitochondrial genome has also been assembled and is 23.25 kilobases in length.

The genome sequence of Tenthredo notha Klug, 1814, a sawfly.

We present a genome assembly from an individual female Tenthredo notha (Arthropoda; Insecta; Hymenoptera; Tenthredinidae). The genome sequence is 253 megabases in span. Most of the assembly (99.91%) is scaffolded into 20 chromosomal pseudomolecules. The mitochondrial genome was also assembled and is 19.8 kilobases in length. Gene annotation of this assembly on Ensembl has identified 10,235 protein coding genes.