Genetic correlation estimates between calving ease in primiparous cows and economically important traits in Nellore cattle.

This study aimed to estimate (co)variance components and genetic parameters for calving ease (CE) and their genetic correlations with growth, reproductive, carcass, and feed efficiency traits in Nellore cattle. Phenotypes for CE are scored in two categories: normal calving and assisted calving. The traits considered were probability of precocious calving, age at first calving, stayability, adjusted scrotal circumference at 365 days of age, accumulated cow productivity, age at puberty of males, gestation length, birth weight, adjusted weights at 210 and 450 days of age, adult cow weight, frame score, hip height, rib eye area, subcutaneous backfat thickness, rump fat thickness, intramuscular fat percentage, residual feed intake and dry matter intake. The estimation of genetic parameters was performed using a two-trait threshold-linear animal model, except for CE, stayability, and probability of precocious calving, which were evaluated through a two-trait threshold animal model. The direct (0.27) and maternal (0.19) heritability estimates for CE in heifers primiparous Nellore indicated that selecting for this trait is feasible. The selection to improve the female sexual precocity should consider CE during the selection and mating decisions to reduce calving problems. Genetic correlation estimates between CE and BW suggest that selecting low birth weight to reduce calving problems is not an appropriate strategy to improve calving ease in heifers Nellore. Therefore, adopting a multi-trait selection model with CE and BW in the Nellore breed would reduce calving difficulties, particularly in sexually precocious heifers, without impairing the growth, reproductive, feed efficiency conversion, and carcass indicator traits.

Genetic association between feed efficiency, growth, scrotal circumference, and carcass traits in Guzerat cattle.

The objective of this study was to estimate the genetic parameters for feed efficiency-related traits and their genetic correlations with growth, male fertility, and carcass traits using multi-trait analysis in Guzerat cattle. Further, it aimed to predict the direct and correlated responses for feed efficiency traits when selection was applied for growth, male fertility, and carcass traits. The evaluated traits were adjusted weight at 120 (W120), 210 (W210), 365 (W365), and 450 days of age (W450), adjusted scrotal circumference at 365 days of age (SC365) and at 450 days of age (SC450), scrotal circumference, ribeye area (REA), backfat thickness (BFT), rump fat thickness (RFT), residual feed intake (RFI), and dry matter intake (DMI). The genetic parameters were obtained by the restricted maximum likelihood method (REML), using an animal model in multi-trait analyses. The heritability estimates for W120, W210, W365, W450, SC365, and SC450 varied from low to high (0.17 to 0.39). The carcass traits, REA, BFT, and RFT, displayed low to moderate heritability estimates, 0.27, 0.10, and 0.31, respectively. The heritability estimates for RFI (0.15) and DMI (0.23) were low and moderate, respectively. The RFI showed low genetic correlations with growth traits, ranging from - 0.07 to 0.22, from 0.03 to 0.05 for scrotal circumference, and from - 0.35 to 0.16 for carcass, except for DMI, which ranged from 0.42 to 0.46. The RFI and DMI presented enough additive genetic variability to be used as selection criteria in Guzerat breed genetic improvement program. Additionally, the response to selection for RFI would be higher when selection is performed directly for this trait. The selection for residual feed intake would not promote unfavorable correlated responses for scrotal circumference, carcass (yield and finish), and growth traits. Therefore, the selection for more efficient animals would not compromise the productive, reproductive, and carcass performance, contributing to reduce the production costs, increasing the profitability and sustainability of beef cattle production in tropical areas.

Genomic prediction ability and genetic parameters for residual feed intake calculated using different approaches and their associations with growth, reproductive, and carcass traits in Nellore cattle.

This study aimed to estimate prediction ability and genetic parameters for residual feed intake (RFI) calculated using a regression equation for each test (RFItest) and for the whole population (RFIpop) in Nellore beef cattle. It also aimed to evaluate the correlations between RFIpop and RFItest with growth, reproductive, and carcass traits. Genotypic and phenotypic records from 8354 animals were used. An analysis of variance (ANOVA) was performed to verify the adequacy of the regression equations applied to estimate the RFItest and RFIpop. The (co)variance components were obtained using the single-step genomic best linear unbiased prediction under single and two-trait animal model analyses. The genetic and phenotypic correlations between RFItest and RFIpop with dry matter intake, frame, growth, reproduction, and carcass-related traits were evaluated. The prediction ability and bias were estimated to compare the RFItest and RFIpop genomic breeding values (GEBV). The RFIpop ANOVA showed a higher significance level (p < 0.0001) than did the RFItest for the fixed effects. The RFIpop displayed higher additive genetic variance estimated than the RFItest, although the RFIpop and RFItest displayed similar heritabilities. Overall, the RFItest showed higher residual correlations with growth, reproductive, and carcass traits, while the RFIpop displayed higher genetic correlations with such traits. The GEBV for the RFItest was slightly biased than GEBV RFIpop. The approach to calculate the RFI influenced the decomposition and estimation of variance components and genomic prediction for RFI. The application of RFIpop would be more appropriate for genetic evaluation purpose to adjust or correct for non-genetic effects and to decrease the prediction bias for RFI.

Genomic prediction for meat and carcass traits in Nellore cattle using a Markov blanket algorithm.

This study was carried out to evaluate the advantage of preselecting SNP markers using Markov blanket algorithm regarding the accuracy of genomic prediction for carcass and meat quality traits in Nellore cattle. This study considered 3675, 3680, 3660 and 524 records of rib eye area (REA), back fat thickness (BF), rump fat (RF), and Warner-Bratzler shear force (WBSF), respectively, from the Nellore Brazil Breeding Program. The animals have been genotyped using low-density SNP panel (30 k), and subsequently imputed for arrays with 777 k SNPs. Four Bayesian specifications of genomic regression models, namely Bayes A, Bayes B, Bayes Cπ and Bayesian Ridge Regression methods were compared in terms of prediction accuracy using a five folds cross-validation. Prediction accuracy for REA, BF and RF was all similar using the Bayesian Alphabet models, ranging from 0.75 to 0.95. For WBSF, the predictive ability was higher using Bayes B (0.47) than other methods (0.39 to 0.42). Although the prediction accuracies using Markov blanket of SNP markers were lower than those using all SNPs, for WBSF the relative gain was lower than 13%. With a subset of informative SNPs markers, identified using Markov blanket, probably, is possible to capture a large proportion of the genetic variance for WBSF. The development of low-density and customized arrays using Markov blanket might be cost-effective to perform a genomic selection for this trait, increasing the number of evaluated animals, improving the management decisions based on genomic information and applying genomic selection on a large scale.

Association of visual scores with reproductive traits in Nelore cattle using Bayesian Inference / Associação de escores visuais com características reprodutivas em bovinos Nelore utilizando Inferência Bayesiana

The use of morphological traits assessed using visual scores as indirect selection criteria in cattle has the advantage of evaluating young animals regarding potential productive and reproductive performance. This enables breeders to make earlier decisions compared to later measurements, such as scrotal circumference at 450 days (SC450) and stayability (STAY). The aim of this study was to estimate the genetic parameters for visual score traits and their associations with reproductive traits: scrotal circumference at 365 days of age (SC365), SC450, STAY, probability of precocious calving (PPC30) and age at first calving (AFC) in Nellore cattle. Visual score data from 4,175 Nellore cattle, with an average age of 22 months, and reproductive data from 3,075 cattle belonging to the HoRa Genetics Provada herd were used. The morphological traits were evaluated by the MERCOS methodology. The heritability estimates obtained ranged from 0.15 to 0.28 for visual scores and 0.10 to 0.54 for reproductive traits. Genetic correlations between visual scores and reproductive traits were generally low, except between: muscularity and PPC30; structure and STAY; racial and SC450; conformation and SC365, SC450, STAY, and AFC; navel and STAY and AFC; and sacrum and SC365, STAY, and AFC, which were moderate to high. The identification of animals with flat sacral bone (not protruding or sloping) can also be an efficient characteristic in the identification for early pregnancy, and together with the musculature score, they can be related to animals with lower age at the first calving.(AU)

A utilização de características morfológicas de bovinos, pelo uso de escores visuais como critério de seleção indireta tem como vantagem a avaliação em animais jovens quanto ao potencial desempenho produtivo e reprodutivo, antecipando a tomada de decisão em comparação a medidas tomadas de forma tardia, como perímetro escrotal aos 450 dias (PE450) e stayability (STAY). Objetivou-se estimar os parâmetros genéticos para características de escores visuais e a associação dessas com características reprodutivas, perímetro escrotal aos 365 (PE365) dias de idade, PE450, STAY, probabilidade de parto precoce (3P) e idade ao primeiro parto (IPP) em bovinos Nelore. Foram utilizadas informações de escores visuais e de reprodução de 4.175 e 3.075 bovinos, respectivamente, com idade média de 22 meses, pertencentes a fazenda HoRa Genética Provada. As características morfológicas foram avaliadas pela metodologia MERCOS. As estimativas de herdabilidade obtidas apresentam grande amplitude, variando de 0,15 a 0,28 para escores visuais e 0,10 a 0,54 para características reprodutivas. As correlações genéticas entre característica de escores visuais e reprodução foram, de maneira geral baixas (0.03-0.66), com exceção entre a musculosidade e 3P, estrutura e STAY, racial e PE450, conformação com PE365, PE450, STAY e IPP, ônfalo com STAY e IPP, e sacro com PE365, STAY e IPP, que foram moderadas a altas. A identificação de animais com melhor osso sacro (mesmo nível das ancas), ou seja, não saliente ou inclinado pode ser uma característica eficiente na identificação para prenhez precoce, e juntamente ao escore de musculatura poderão ser relacionados a animais com menor idade ao primeiro parto.(AU)

Heritability and genetic correlations between marbling in longissimus dorsi muscle and conventional economic traits in Nellore beef cattle.

In Nellore beef cattle, studies addressing genetic correlations between ultrasound marbling content and other economically important traits are still incipient. Therefore, this work aimed to estimate heritability and genetic correlations between ultrasound marbling content in the longissimus dorsi muscle (MARB) and growth, reproductive, feed efficiency, and carcass-related traits in a Nellore beef cattle population from Brazil. Phenotypic records of 614,395 Nellore animals were used and included adjusted weight at 210 (W210) and 450 (W450) days of age, adult cow weight (AW), early heifer pregnancy (EH), stayability (STAY), adjusted scrotal circumference at 365 days of age (SC365), ribeye area (REA), subcutaneous backfat thickness (BF), rump fat thickness (RF), and marbling (MARB). The genetic parameters for all traits but EH and STAY were estimated considering a linear animal model, whereas for those two nonlinear traits, a threshold animal model was used. The direct and correlated response to selection for MARB versus the other traits, and the relative efficiency of selection, were also calculated. The heritability estimate for MARB was 0.31 and for the other conventional evaluated traits was low to moderate, with values ranging from 0.14 to 0.41. The genetic correlations between MARB and growth, reproductive, feed efficiency, and carcass-related trait were very low, with values close to zero, with similar correlated responses. The MARB displayed adequate genetic variability to respond to selection and crossbreeding programs looking forward to higher meat quality and differential market standards for the Nellore beef. The selection for growth, reproductive, feed efficiency, and carcass-related traits would not affect MARB in Nellore beef cattle and vice versa. Therefore, this trait should be included as a selection criterion in the Nellore breeding program.

Milheto como silagem comparado a gramíneas tradicionais: aspectos quantitativos, qualitativos e econômicos / Millet silage compared to traditional grasses: quantitative, qualitative, and economic characteristics

Foram avaliados a composição morfológica, os parâmetros agronômicos, a produção de massa seca das plantas forrageiras utilizadas para o processo de ensilagem, bem como a caracterização do processo fermentativo, em diferentes tempos de abertura dos mini-silos (3, 7, 15, 30, 60, 120 dias) após a ensilagem. Procedeu-se também às determinações das perdas de efluentes e gases, além da composição bromatológica e fracionamento de proteína das silagens. Também foi estimado o custo de produção e a produção de leite em função da matéria seca das silagens produzidas a partir de diferentes gramíneas. As espécies forrageiras avaliadas foram: milheto ADR-7010, sorgo BRS 610, milho AG 5055 com espiga, milho AG 5055 sem espiga e cana-de-açúcar IAC 86-2480. Foram observadas diferenças significativas tanto para produção, quanto composição bromatológica e custos de produção. O milheto não se mostrou competitivo com as demais forrageiras; entretanto, constitui opção como cultura de inverno em decorrência de sua baixa exigência hídrica quando comparado às demais culturas.(AU)

In this assay, we evaluated morphological composition, agronomic parameters, dry matter yield, and the silage fermentative process from pearl millet, sorghum, and corn plant with or without ears within different silos opening times (3, 7, 15, 30, 60, and 120 days). Effluent and gas release from silages were also evaluated besides its chemical composition and protein fractioning. Production costs of all silages were estimated. We also estimate milk production according to different silages dry matter production. Evaluated cultivars were, ADR-7010 (pearl millet), BRS-610 (sorghum), AG-5055 (corn), and IAC-86-2480 (sugarcane). Significant differences were observed for both production as well as chemical composition and production costs. Pearl millet did not prove to be competitive when compared with other forages. However, as winter crop, pearl millet could be profitable due to the low water exigencies when compared to the other crops.(AU)

Milheto como silagem comparado a gramíneas tradicionais: aspectos quantitativos, qualitativos e econômicos / Millet silage compared to traditional grasses: quantitative, qualitative, and economic characteristics

Foram avaliados a composição morfológica, os parâmetros agronômicos, a produção de massa seca das plantas forrageiras utilizadas para o processo de ensilagem, bem como a caracterização do processo fermentativo, em diferentes tempos de abertura dos mini-silos (3, 7, 15, 30, 60, 120 dias) após a ensilagem. Procedeu-se também às determinações das perdas de efluentes e gases, além da composição bromatológica e fracionamento de proteína das silagens. Também foi estimado o custo de produção e a produção de leite em função da matéria seca das silagens produzidas a partir de diferentes gramíneas. As espécies forrageiras avaliadas foram: milheto ADR-7010, sorgo BRS 610, milho AG 5055 com espiga, milho AG 5055 sem espiga e cana-de-açúcar IAC 86-2480. Foram observadas diferenças significativas tanto para produção, quanto composição bromatológica e custos de produção. O milheto não se mostrou competitivo com as demais forrageiras; entretanto, constitui opção como cultura de inverno em decorrência de sua baixa exigência hídrica quando comparado às demais culturas.

In this assay, we evaluated morphological composition, agronomic parameters, dry matter yield, and the silage fermentative process from pearl millet, sorghum, and corn plant with or without ears within different silos opening times (3, 7, 15, 30, 60, and 120 days). Effluent and gas release from silages were also evaluated besides its chemical composition and protein fractioning. Production costs of all silages were estimated. We also estimate milk production according to different silages dry matter production. Evaluated cultivars were, ADR-7010 (pearl millet), BRS-610 (sorghum), AG-5055 (corn), and IAC-86-2480 (sugarcane). Significant differences were observed for both production as well as chemical composition and production costs. Pearl millet did not prove to be competitive when compared with other forages. However, as winter crop, pearl millet could be profitable due to the low water exigencies when compared to the other crops.

Production and chemical composition of hybrid Brachiaria cv. Mulato II under a system of cuts and nitrogen fertilization / Produção e composição bromatológica da Brachiaria híbrida cv. Mulato II sob regime de cortes e adubação nitrogenada

The production capacity of green and dry mass of the entire plant, efficiency of N conversion, apparent N recovery and the chemical composition of cultivar Mulato II was evaluated under a system of cuts and nitrogen doses. The assay, conducted in the municipality of Goiânia, GO, Brazil, had a totally randomized 2 x 4 factorial design (2 height cuts, 0.40 and 0.50 m and 4 nitrogen doses), with three replications and subdivided subplots. Treatments comprised four N doses (0, 50, 100 and 150 kgha-1 N, with urea as nitrogen source). There was no significant interaction (p>0.05) between N doses and cut heights for the variables productivity of green (PGM) and dry (PDM) mass, nitrogen use efficiency (NUE) and apparent N recovery (ANR), which were neither affected (p<0.05) by N doses nor by evaluated cut heights. Average productivity reached 59,450 kgha-1 (PGV) and 10,367 kgha-1 (PDM) and it was produced an average of 19.62 kg of DM per kg of N, with a mean 56.00% recovery. N doses and cut heights did not affect (p>0.05) DM rates of the plant, whilst mean dry matter rate was 17.49%. CP rates were affected (p<0.05) by N doses (0, 50, 100 and 150 kgha-1) and cut heights (0.40 and 0.50 m) and by the interaction of these factors. CP rates of the entire plant hybrid Brachiaria cv. Mulato II increased (p<0.05) due to N doses through an increasing linear relationship. Since there was a significant effect (p<0.05) with regard to cut height and CP rates decreased with height increase. NDF rates were significantly influenced by N doses (p<0.05) and by cut heights (p<0.05), with significance for the interaction (p<0.05) of over 100 kgha-1 N doses only. No significant interaction (p>0.05) occurred in ADF rates among the variables analyzed. ADF contents were influenced by N supply (p<0.05) with decreasing quadratic regression as N doses increased.

O presente trabalho de pesquisa teve como objetivo avaliar o potencial produtivo de massa verde e seca da planta inteira, a eficiência de conversão do N, a recuperação aparente do N, bem como a composição bromatológica do cultivar Mulato II, sob regime de cortes e submetido a doses de nitrogênio, no município de Goiânia, GO, Brasil. Utilizou-se um delineamento experimental inteiramente casualizado com arranjo fatorial 2 X 4 (2 alturas de corte (0,40 e 0,50 m) X 4 doses de nitrogênio) com três repetições e parcelas subdivididas. Os tratamentos foram constituídos por quatro doses de N (0, 50, 100 e 150 kgha-1 de N) (sendo a fonte ureia). Não ocorreu interação significativa (p>0,05) entre doses de N e alturas de corte para as variáveis produtividades de massa verde (PMV) e massa seca (PMS), eficiência de conversão aparente de nitrogênio (ECAN) e recuperação aparente de nitrogênio (RAN), que não foram influenciadas (p<0,05) pelas doses de N, nem em função das alturas de corte avaliadas. A média de produtividade encontrada foi de 59.450 kgha-1 (PMV) e 10.367 kgha-1 (PMS), produzindo em média 19,62 kg de MS para cada kg de N aplicado, com uma recuperação média de 56,00%. As doses de N aplicadas e as alturas de corte não influenciaram (p>0,05) os teores de MS da planta. A média do teor de matéria seca encontrada foi de 17,49%. Os teores de PB foram influenciados (p<0,05) pelas doses de N (0, 50, 100 e 150 kgha-1) e alturas de corte (0,40 e 0,50 m) bem como a interação desses fatores. Os teores de proteína bruta (PB) da planta inteira Brachiaria híbrida cv. Mulato II aumentaram (p<0,05) em função das doses de N, apresentando uma relação linear crescente. Quanto à altura de corte, ocorreu também efeito significativo (p<0,05), evidenciando que os teores de PB diminuíram com o aumento da altura. Os teores de FDN foram influenciados significativamente pelas doses de N (p<0,05) e pelas alturas de corte (p<0,05), apresentando significância para a interação (p<0,05) apenas nas doses acima de 100 kgha-1 de N. Para os teores de FDA não houve interação significativa (p>0,05) entre as variáveis analisadas. O conteúdo de FDA foi influenciado pelo fornecimento de N (p<0,05), apresentando regressão quadrática decrescente com o aumento das doses de N.

Introduction of forage legumes, liming and phosphorus in degraded Brachiaria brizantha pastures / Introdução de leguminosas forrageiras, calagem e fosfatagem em pastagem degradada de "Brachiaria brizantha"

There is a need to intensify livestock productivity to feed the growing world population. Therefore, this study aimed to evaluate the intercropping of Brachiaria pastures with Estilosantes and Calopogonio under application of lime and phosphate. Thus, was evaluated the organic and dry matter production, tiller density, dry matter and mineral content  in degraded Brachiaria brizantha pastures, applying lime and phosphate and introducing legumes. The entirely randomized experimental design was used and a split plot scheme, with two types of legumes (Estilosantes Campo Grande and Calopogonio) and three strategies of fertilization (Control, CaCO3, CaCO3 + P). The lime and phosphate were performed on the pasture surface without incorporation. The introduction of legumes was carried by no-tillage practices after overgrazing. The evaluations were performed when the dossal reached 25 cm height with the assistance of a 0.25 m2 square. Legumes did not differ for the natural and dry matters production of Brachiaria brizantha (P > 0.05), but the correction and fertilization factors (CaCO3 + P) improved significantly (P 0.05) the pastures mass production when the Estilosantes Campo Grande were usedin intercropping. The results show that is possible to intensify livestock productivity using forage legumes, associated with CaCO3 + P fertilization, to improve and recovery productivity

Existe a necessidade de intensificar a produtividade pecuária para alimentar a crescente população mundial. Sendo assim, objetivou-se avaliar a consorciação de pastagens Brachiaria com Estilosantes e Calopogônio sob aplicação de calagem e fosfatagem. Avaliou-se a produção de matéria orgânica e seca, densidade de perfilhos, teor de matéria seca e matéria mineral em pastagens degradadas de Brachiaria brizantha, com aplicação de calagem, fosfatagem e introdução de leguminosas. O delineamento experimental utilizado foi o inteiramente casualizado com parcelas subdividida, em que foram avaliados dois tipos de leguminosas (Estilosantes Campo Grande e Calopogônio) e três tipos de adubação (Controle, CaCo3, CaCo3 + P). A calagem e fosfatagem foram realizadas na superfície da pastagem sem incorporação. A introdução das leguminosas foi feita em plantio direto após superpastejo. As avaliações foram realizadas quando o dossel atingiu 25 cm de altura com auxílio de um quadrado de 0,25 m2. As leguminosas não diferiram para produção de matéria natural e seca das pastagens de Brachiaria brizantha (P > 0,05), mas os fatores de correção e adubação (CaCo3 + P) melhoraram significativamente (P 0,05) a produção de massa das pastagens quando utilizou-se o Estilosantes Campo Grande em consorciação. Os resultados indicam a possibilidade de intensificar a produtividade pecuária usando leguminosa

Introduction of forage legumes, liming and phosphorus in degraded Brachiaria brizantha pastures / Introdução de leguminosas forrageiras, calagem e fosfatagem em pastagem degradada de "Brachiaria brizantha"

There is a need to intensify livestock productivity to feed the growing world population. Therefore, this study aimed to evaluate the intercropping of Brachiaria pastures with Estilosantes and Calopogonio under application of lime and phosphate. Thus, was evaluated the organic and dry matter production, tiller density, dry matter and mineral content  in degraded Brachiaria brizantha pastures, applying lime and phosphate and introducing legumes. The entirely randomized experimental design was used and a split plot scheme, with two types of legumes (Estilosantes Campo Grande and Calopogonio) and three strategies of fertilization (Control, CaCO3, CaCO3 + P). The lime and phosphate were performed on the pasture surface without incorporation. The introduction of legumes was carried by no-tillage practices after overgrazing. The evaluations were performed when the dossal reached 25 cm height with the assistance of a 0.25 m2 square. Legumes did not differ for the natural and dry matters production of Brachiaria brizantha (P > 0.05), but the correction and fertilization factors (CaCO3 + P) improved significantly (P 0.05) the pastures mass production when the Estilosantes Campo Grande were usedin intercropping. The results show that is possible to intensify livestock productivity using forage legumes, associated with CaCO3 + P fertilization, to improve and recovery productivity(AU)

Existe a necessidade de intensificar a produtividade pecuária para alimentar a crescente população mundial. Sendo assim, objetivou-se avaliar a consorciação de pastagens Brachiaria com Estilosantes e Calopogônio sob aplicação de calagem e fosfatagem. Avaliou-se a produção de matéria orgânica e seca, densidade de perfilhos, teor de matéria seca e matéria mineral em pastagens degradadas de Brachiaria brizantha, com aplicação de calagem, fosfatagem e introdução de leguminosas. O delineamento experimental utilizado foi o inteiramente casualizado com parcelas subdividida, em que foram avaliados dois tipos de leguminosas (Estilosantes Campo Grande e Calopogônio) e três tipos de adubação (Controle, CaCo3, CaCo3 + P). A calagem e fosfatagem foram realizadas na superfície da pastagem sem incorporação. A introdução das leguminosas foi feita em plantio direto após superpastejo. As avaliações foram realizadas quando o dossel atingiu 25 cm de altura com auxílio de um quadrado de 0,25 m2. As leguminosas não diferiram para produção de matéria natural e seca das pastagens de Brachiaria brizantha (P > 0,05), mas os fatores de correção e adubação (CaCo3 + P) melhoraram significativamente (P 0,05) a produção de massa das pastagens quando utilizou-se o Estilosantes Campo Grande em consorciação. Os resultados indicam a possibilidade de intensificar a produtividade pecuária usando leguminosa(AU)