RESUMEN
It was assumed that dietary inclusion of Lactobacillus reuteri SL001 isolated from the gastric contents of rabbits could act as an alternative to feed antibiotics to improve the growth performance of broiler chickens. We randomly assigned 360 one-day-old AA white-feathered chicks in three treatments: basal diet (control), basal diet plus zinc bacitracin (antibiotic), and basal diet plus L. reuteri SL001 (SL001) treatment. The results showed the total BW gain and average daily gain (ADG) of broilers in SL001 treatment increased significantly (p < 0.05, respectively) compared with the control group from day 0 to 42. Moreover, we observed higher levels of immune globulins in both the SL001 group and the antibiotic group. Total antioxidant capacity and levels of antioxidant factors were also significantly increased (p ≤ 0.05, respectively) in the SL001 treatment group, while the interleukin 6, interleukin 4, creatinine, uric acid, total cholesterol, triglyceride, VLDL, LDL and malondialdehyde were remarkably decreased (p < 0.05, respectively). In the ileum of SL001 treatment broilers, the height of villi and the ratio of villi height to crypt depth were significantly increased (p < 0.05). Meanwhile, the crypt depth reduced (p < 0.01) and the ratio of villi height to crypt depth increased (p < 0.05) in the jejunum compared to the control. The abundance of microbiota increased in the gut of broilers supplemented with SL001. Dietary SL001 significantly increased the relative abundance of Actinobacteria in the cecal contents of broilers (p < 0.01) at the phylum level. In conclusion, L. reuteri SL001 supplementation promotes the growth performance of broiler chickens and exhibits the potential application value in the industry of broiler feeding.
RESUMEN
This study was conducted to investigate the effects of supplementing Bacillus subtilis and an antibiotic (Zinc bacitracin) in the diet of broilers on growth performance, organ weight, blood metabolites, and digestive enzymes of broiler chickens. A total of 600 1-d Arbor Acres broilers were randomly allotted to five treatments. Each treatment consisted of six replicates with four pens, and each pen had five birds. The chicks were fed (1) the basal diet (control), (2) the basal diet with 500 mg/kg Zinc bacitracin (APZ), (3) the basal diet with B. subtilis at 1 × 108 CFU/g (B.Sut-1), (4) the basal diet with B. subtilis at 3 × 108 CFU/g (B.Sut-3), and (5) the basal diet with B. subtilis at 5 × 108 CFU/g (B.Sut-5). The experiment lasted for 42 days. In this study, the supplementation of diets with B. subtilis (B.Sut-3 and B.Sut-5 groups) increased body weight gain from 1 to 21 days compared with control (p < 0.05). Additionally, the B.Sut-3 group had a significantly heavier bursa of Fabricius than control at 21 days (p < 0.05). Serum total protein, albumin, and high-density lipoprotein concentrations were increased in B.Sut-5 and APZ groups (p < 0.05) over the whole period. Serum low-density lipoprotein, very low-density lipoprotein, triglyceride, and total cholesterol concentrations were decreased in B.Sut-5 and APZ groups at 21 and 42 days (p < 0.05). Chicks in the B.Sut-5 and APZ groups had higher serum lipase, pepsin, and amylase activities (p < 0.05) at 21 and 42 days. From the results obtained from the study, it can be concluded that Bacillus subtilis ATCC19659 at 5 × 108 CFU/g could be applied as an alternative to antibiotics in poultry diets.
RESUMEN
This study investigated dietary supplementation with Bacillus subtilis (BS) ATCC19659 on growth performance, biochemical indices, intestinal morphology, and cecum microflora in broiler chicks. A total of 600 Arbor 1-day Acres broilers of either sex were allotted to 5 treatments: chicks were fed a corn- and soybean-based diet (CON); chicks were fed basal diet containing 500 mg ZnB/kg (ZnB); chicks were fed basal diet containing 1 × 108 CFU/g feed of BS-ATCC19659 (BS-1); chicks were fed basal diet containing 3 × 108 CFU/g feed of BS-ATCC19659 (BS-3); and chicks were fed basal diet containing 5 × 108 CFU/g feed of BS-ATCC19659 (BS-5). Each treatment comprised 6 replicates with 20 birds for each replicate pen. Chicks in the BS-5 and BS-3 groups had higher body weight at the 21st and 42nd days and average daily gain from 1 to 21 days than that in the CON group (p < 0.05). Chicks in the BS-5 and ZnB groups had higher serum antioxidant activities and immunity response than those in the CON group (p < 0.05). Compared with the CON group, the liver mRNA abundance of GHR, TGF-ß, IGF-1, IFN-γ, SOD, CAT, and GPX of chicks in three BS groups and the ileum villus length (µm) of chicks in BS-3 and ZnB groups was increased (p < 0.05). Compared with the CON group, the villus height-to-crypt depth ratio of the ileum of chicks in the BS-5 and BS-3 groups and the crypt depth and villus height-to-crypt depth ratio of the jejunum in the BS-5 and ZnB groups were increased (p < 0.05). The abundance of the Cyanobacteria phyla in the cecum decreased in response to treatment with both BS-ATCC19659 and ZnB groups (p < 0.05). Compared with the CON group, the cecum abundance of genera GCA-900066575 (Lachnospiraceae), Anaerofustis, and Papillibacter (Firmicutes phylum) in three BS groups were increased (p < 0.05); The abundance of genus Escherichia-Shigella reduced in the BS-3 group (p < 0.05). Compared with the CON group, the cecum abundance of genus Clostridia_unclassified in ZnB and BS-5 groups was decreased (p < 0.05) of broilers. Generally, Bacillus subtilis ATCC19659 as feed additive positively affected growth performance, immunity response, and cecal microflora of broilers.