Peculiarities of the effects of bile acids on atpase activity of the colon mucosa in patients with overweight and irritable bowel syndrome.

OBJECTIVE: The aim is to investigate the effect of bile acids on the ATPase activity of the colon mucosa in patients with overweight and irritable bowel syndrome (IBS). PATIENTS AND METHODS: Materials and methods: Completely examined 12 patients with IBS and overweight.We estimated the ATPase activity of colon mucous of the patients with IBSspectrophotometrically by determined the content of orthophosphate that was released after ATP hydrolysis. We studied the effect of 3-sulphate of taurolitocholate (TLC-S) on specific activities of Na+/ K+-ATPase, Ca2+-ATPase of endoplasmatic reticulum (EPR),Ca2+-ATPase of plasmatic membrane (PM) and basal Mg2+-ATPase of postmitochondrial subcellular fraction of colon mucous of the patients with IBS. RESULTS: Results: We establishedthe specific activities of Na+/K+-ATPase, Ca2+-ATPase of EPR,Ca2+-ATPase of PM and basal Mg2+-ATPase. Therewere(6.06 ± 1.61), (5.88 ± 1.19), (8.86 ± 1.56) (6.44 ± 2.02)µmol Pi/ mg protein per hour, respectively. TLC-S (50 µM) did not causedany change of Na+/K+-ATPase , as well as Ca2+-ATPasesactivities, but statistically significant increased activity of Mg2+-ATPase of postmitochondrial subcellular fraction of colon mucous of the patients with IBS by 4 fold. CONCLUSION: Conclusions: TLC-S increased basal Mg2+-ATPase in the postmitochondrial fraction of colon mucous of the patients with overweight and IBS, but had no effect on Na+/K+-ATPase and Ca2+-ATPases activities. It has been suggested that activation of basal Mg2+-ATPase under by TLC-S may indicates the role of the endo-lysosomal system of epitheliocytes of colon mucous in developing of pathology IBS.

A new method of diagnosis of the hiatal hernia associated with gastroesophageal reflux disease.

OBJECTIVE: Introduction: Gastroesophageal reflux disease (GERD) is one of the most common gastroduodenal diseases. The relationship between the hiatal hernia and the GERD is established. It is advisable to develop an accessible non-invasive diagnostic method for this combined pathology. The aim of the research was to estimate measuring of calcium in patients' saliva samples as simple non-invasive diagnostic method of GERD associated with the hiatal hernia. PATIENTS AND METHODS: Materials and methods: The samples of saliva were obtained from 37 patients with hiatal hernia associated with GERD and 22 healthy volunteers. The content of calcium in saliva was measured using calcium-sensitive dye Arsenazo III by photometrical method at a wavelength of 590-650 nm. RESULTS: Results: It has been established that in the saliva of patients with hiatal hernia, the calcium content was increased by 100.9% compared to the control group. Such a significant increase in the level of calcium in the saliva of patients with hiatal hernia may be due to the fact that the development of this pathology is a disorder of calcium homeostasis. CONCLUSION: Conclusions: It has been found that the calcium content in the saliva of patients with hiatal hernia exceeded the norm almost twice. Thus, the determination of calcium content in saliva can be used as a simple non-invasive diagnostic marker of hiatal hernia associated with GERD.

[Features of the course of irritable bowel syndrome in patients with excess body weight].

OBJECTIVE: Introduction: Currently, the diagnosis of irritable bowel syndrome (IBS) is only based on clinical symptoms without clear interpretation and can be interpreted differently, complicating the diagnosis. Therefore, to study features of the prevalence and the course of IBS in patients with excessive body weight / obesity is of particular significance nowadays. The aim of the research is to evaluate the prevalence and the course of IBS in patients with obesity. PATIENTS AND METHODS: Materials and methods: Observation results for 120 obese persons who underwent in-patient treatment at various hospital departments in Western Ukraine have been analyzed to evaluate the prevalence of IBS. A thorough collection of complaints, anamnesis of the disease and lifestyle were conducted due to the survey; objective survey data were analyzed. The diagnosis of IBS was made according to Rome IV diagnostic criteria (2016). RESULTS: Results: Patients of all groups were gender and age-matched participants. The average body mass index was 33.9 ± 0.83 kg / m². Excessive body weight was revealed in 6 patients (5.0%). Degrees of obesity: (stage I) was diagnosed in 74 patients (61.6%), stage II was determined in 29 patients (24.2%), and stage III - in 11 patients (9,2%). The vast majority of patients (82.5%) complained of fullness or discomfort in the abdomen. Every second obese patient (52.5%) noted periodic bloating. Periodic nausea was recorded in every third patient with obesity (32.5%). Recurrent abdominal pain was determined in 62 (51.7%) of patients during the last 3 months: in females - 38 (61.3%), in males - 24 (38.7%) respectively. The prevalence of diarrhea with IBS compared to IBS with constipation was also noted: 36 (58.1%) versus 26 (41.9%) of cases. CONCLUSION: Conclusions: Irritable bowel syndrome was revealed in 51.7% of obese patients. Significant prevalence of combined pathology was noted in females. The prevalence of irritable bowel syndrome with diarrhea was determined.