Mitochondrial division inhibitor (mdivi-1) induces extracellular matrix (ECM)-detachment of viable breast cancer cells by a DRP1-independent mechanism.

Increasing evidence supports the hypothesis that cancer progression is under mitochondrial control. Mitochondrial fission plays a pivotal role in the maintenance of cancer cell homeostasis. The inhibition of DRP1, the main regulator of mitochondrial fission, with the mitochondrial division inhibitor (mdivi-1) had been associated with cancer cell sensitivity to chemotherapeutics and decrease proliferation. Here, using breast cancer cells we find that mdivi-1 induces the detachment of the cells, leading to a bulk of floating cells that conserved their viability. Despite a decrease in their proliferative and clonogenic capabilities, these floating cells maintain the capacity to re-adhere upon re-seeding and retain their migratory and invasive potential. Interestingly, the cell detachment induced by mdivi-1 is independent of DRP1 but relies on inhibition of mitochondrial complex I. Furthermore, mdivi-1 induces cell detachment rely on glucose and the pentose phosphate pathway. Our data evidence a novel DRP1-independent effect of mdivi-1 in the attachment of cancer cells. The generation of floating viable cells restricts the use of mdivi-1 as a therapeutic agent and demonstrates that mdivi-1 effect on cancer cells are more complex than anticipated.

Validity of the Maturation Stage Method in the Individual Assessment of Midpalatal Suture Ossification Before Maxillary Expansion: A Systematic Review / Validez del Método de la Etapa de Maduración en la Evaluación Individual de la Osificación de la Sutura Palatina Mediana Previo a la Expansión Maxilar: Una Revisión Sistemática

In 2013, midpalatal suture maturation stage assessment was proposed for the evaluation of patients before performing maxillary expansion. In this study, we aimed to analyze the association between the midpalatal suture maturation stages assessed by CBCT, according to the method described by Angelieri et al., and other objective methods used to assess skeletal maturation or bone fusion. A computerized database search was conducted using PubMed, Cochrane Library, SciELO, LILACS, Web of Science, and Scopus, without language restriction. Unpublished literature was searched on ClinicalTrials.gov, the National Research Register, and Pro-Quest Dissertation Abstracts and Thesis database. Authors were contacted when necessary, and reference lists of the included studies were screened. Search terms included midpalatal suture, maturation, correlation, diagnostic performance, classification, evaluation, assessment, and relationship. Quality assessment was performed using the Observational Cohort and Cross-Sectional Studies tool developed by the National Heart, Lung, and Blood Institute. Eleven studies met the inclusion criteria. Of all the studies included, 81.9% had fair qualit y and 18.1% good quality, respectively. Eight out of eleven studies assessed the correlation between the midpalatal suture maturation method and the skeletal maturity evaluated by CVM method (Spearman's correlation coefficient: 0.244-0.908). Two out of eleven studies evaluated the correlation between midpalatal suture maturation method and the skeletal maturity assessed by HWM method (Spearman's correlation coefficient: 0.904-0.905) Even though midpalatal suture maturation stage assessment needs an exhaustive training and calibration process, it is a valid method to evaluate skeletal maturation or bone fusion. From a clinical perspective, for patients at CS4, CS5 and CS6, an assessment of the midpalatal suture on CBCT is indicated. A similar assessment should be done in patients at SMI 7-9.

En 2013, se propuso un nuevo método para la evaluación del estadio de maduración de la sutura palatina mediana para la evaluación de los pacientes antes de realizar la expansión maxilar. En este estudio, nuestro objetivo fue analizar la asociación entre las etapas de maduración de la sutura palatina mediana evaluada en CBCT, según el método descrito por Angelieri et al., y otros métodos objetivos utilizados para evaluar la maduración esquelética o la fusión ósea. Se realizó una búsqueda en las bases de datos PubMed, Cochrane Library, SciELO, LILACS, Web of Science y Scopus, sin restricción de idioma. Se buscó literatura no publicada en ClinicalTrials.gov, el Registro Nacional de Investigación y la base de datos Pro-Quest Dissertation Abstracts and Thesis. Se estableció contacto con los autores cuando fue necesario y se revisaron las listas de referencias de los estudios incluidos. Los términos de búsqueda incluyeron sutura palatina mediana, maduración, correlación, rendimiento diagnóstico, clasificación, evaluación, valoración y relación. La evaluación de la calidad se realizó mediante la herramienta de Estudios transversales y de cohortes observacionales desarrollada por el Instituto Nacional del Corazón, los Pulmones y la Sangre. Once estudios cumplieron con los criterios de inclusión. Del total de estudios incluidos, el 81.9% tuvo calidad regular y el 18.1% calidad buena, respectivamente. Ocho de once estudios evaluaron la correlación entre el método de maduración de la sutura palatina mediana y la madurez esquelética evaluada por el método CVM (coeficiente de correlación de Spearman: 0.244-0.908). Dos de once estudios evaluaron la correlación entre el método de maduración de la sutura palatina mediana y la madurez esquelética evaluada por el método HWM (coeficiente de correlación de Spearman: 0.904-0.905). Aunque la evaluación del estado de maduración de la sutura palatina mediana necesita un proceso exhaustivo de entrenamiento y calibración, es un método válido para evaluar la maduración esquelética o la fusión ósea. Desde una perspectiva clínica, para pacientes en CS4, CS5 y CS6, está indicada una evaluación de la sutura palatina mediana en CBCT. Se debe realizar una evaluación similar en pacientes con SMI 7-9.

Characterization of Mandibular Border Movements and Mastication in Each Skeletal Class Using 3D Electromagnetic Articulography: A Preliminary Study.

Mandibular movement recording is relevant for the planning and evaluation of mandibular function. These movements can include mandibular border movements (MBM) or mastication. Our objective was to characterize the kinematics of MBM and mastication among skeletal classes I, II, and III in the three spatial planes. A descriptive cross-sectional study was conducted with 30 participants. Instructions were provided on how to form Posselt's envelope and to perform masticatory. After data processing, we obtained numerical values for the areas, trajectories, and ranges of MBM that formed Posselt's envelope and the values for speed, masticatory frequency, and the areas of each masticatory cycle. Significant differences were found in the area of Posselt's envelope in the horizontal plane between skeletal classes I and III and in the range of right laterality between skeletal classes II and III. Mastication showed significant differences in the area of the masticatory cycles in the horizontal plane between classes I and III and between classes II and III. In conclusion, there were differences in MBM and mastication between skeletal classes III and I in the horizontal plane. This study supports the need to establish normal values for mandibular kinematics in skeletal class III.

Midpalatal Suture Maturation Stage in 10- to 25-Year-Olds Using Cone-Beam Computed Tomography-A Cross-Sectional Study.

In this study, we aimed to evaluate the frequency of midpalatal maturational stages in a Chilean urban sample of adolescents, post-adolescents and young adults, associated with chronological age and sex, by assessing CBCT scan images. Tomographic images in axial sections of the midpalatal sutures from 116 adolescents and young adults (61 females and 55 males, 10-25 years old) were classified according to their morphologic characteristics in five maturational stages (A, B, C, D and E), as proposed by Angelieri et al. The sample was divided into three groups: adolescents, post-adolescents and young adults. Three previously calibrated examiners (radiologist, orthodontist and general dentist) analyzed and classified the images. Stages A, B and C were considered to be an open midpalatal suture, and D and E were considered to be a partially or totally closed midpalatal suture. The most frequent stage of maturation was D (37.9%), followed by C (24%) and E (19.6%). The possibility of finding closed midpalatal sutures in individuals of 10 to 15 years was 58.4%, and in subjects aged 16 to 20 and 21 to 25 years, it was 51.7% and 61.7%, respectively. In males, Stages D and E were present in 45.4%; for females, this prevalence was 68.8%. Individual assessment of the midpalatal suture in each patient is of crucial importance before making the clinical decision of which is the best maxillary expansion method. Due to the extensive calibration and training required, it is advisable to always request a report from a radiologist. Individual evaluation with 3D imaging is recommended because of the great variability observed in the ossification of midpalatal sutures in adolescents, post-adolescents and young adults.

The aging of ER-mitochondria communication: A journey from undifferentiated to aged cells.

The complex physiology of eukaryotic cells requires that a variety of subcellular organelles perform unique tasks, even though they form highly dynamic communication networks. In the case of the endoplasmic reticulum (ER) and mitochondria, their functional coupling relies on the physical interaction between their membranes, mediated by domains known as mitochondria-ER contacts (MERCs). MERCs act as shuttles for calcium and lipid transfer between organelles, and for the nucleation of other subcellular processes. Of note, mounting evidence shows that they are heterogeneous structures, which display divergent behaviors depending on the cell type. Furthermore, MERCs are plastic structures that remodel according to intra- and extracellular cues, thereby adjusting the function of both organelles to the cellular needs. In consonance with this notion, the malfunction of MERCs reportedly contributes to the development of several age-related disorders. Here, we integrate current literature to describe how MERCs change, starting from undifferentiated cells, and their transit through specialization, malignant transformation (i.e., dedifferentiation), and aging/senescence. Along this journey, we will review the function of MERCs and their relevance for pivotal cell types, such as stem and cancer cells, cardiac, skeletal, and smooth myocytes, neurons, leukocytes, and hepatocytes, which intervene in the progression of chronic diseases related to age.

Non-Syndromic Familial Mesiodens: Presentation of Three Cases.

Mesiodens are the most common supernumerary teeth and are detected incidentally during routine radiographic examination, so late diagnosis complications are very common. The dentist must make a timely diagnosis and thus avoid clinical complications. Despite advances in knowledge of dental morphogenesis and differentiation, the etiology of mesiodens remains unclear. Therefore, several theories have been postulated to explain how and why they develop. It was described in the literature that heredity could play an important role in the appearance of supernumerary teeth, with a higher rate of appearance in relatives of those affected. This article reports three cases, a mother and two children, who present mesiodens, which shows that supernumerary teeth may involve a genetic factor. In addition, a literature review was carried out to assess the importance of the genetic factor as a possible cause of mesiodens. The relevance and implications of timely diagnosis in clinical practice to avoid manifestations of clinical complications are discussed. Therefore, the identification of the genetic risk factors responsible for the formation of supernumerary teeth is essential for developing a screening tool to determine an individual's genetic risk.

Bullying a personas con trastornos de la comunicación integradas en establecimientos escolares: rol de la fonoaudiología y sus implicancias en la salud comunicativa / Bullying of people with communication disorders integrated in schools: role of speech therapy and its implications for communicative health

El bullying es un fenómeno definido como una forma de agresión intencional que incluye actos verbales, físicos o psicológicos que ocurren en una relación con otras personas de manera reiterada, sistemática y sostenida en el tiempo. La mayor parte de los estudios se concentran en el bullying y las consecuencias en población escolar normotípica, sin embargo, existe poca exploración en el bullying que es resultado de un trastorno comunicativo. Es por ello que la labor de los fonoaudiólogos insertos en ámbitos educativos no es clara en explicitar los lineamientos de acciones terapéuticas que favorezcan la prevención de este fenómeno. El propósito de esta investigación es conocer las principales concepciones que los fonoaudiólogos tienen sobre el bullying en personas con trastornos de la comunicación en edad escolar. Con este objetivo, se realizó una investigación con métodos cuantitativos y cualitativos, mediados por encuestas y entrevistas en profundidad respectivamente. Se encontró que más del 65% del bullying entre personas dentro de los establecimientos educacionales se relaciona con niños de entre 9 y 12 años de edad con trastornos de la comunicación. En cuanto a la autopercepción del rol fonoaudiológico en el tema de la prevención e/o intervención en temas de bullying, emergen los conceptos de favorecer los aspectos del lenguaje y/o del habla en los niños que tienen dificultad, la sensibilización de los actores involucrados, el refuerzo del autoestima y el trabajo en equipo interdisciplinario. Finalizando el tratamiento analítico de las entrevistas, se establece que la Fonoaudiología cumpliría un papel fundamental en el abordaje del bullying en poblaciones con trastornos de la comunicación, siendo el profesional que actúa directamente sobre la salud comunicativa.

Bullying is a phenomenon defined as a form of intentional aggression that includes verbal, physical or psychological acts that occur in a relationship with other people in a repeated, systematic and sustained manner over time. Most of the studies focus on bullying and its consequences in the normal school population, however, there is little exploration of bullying that is the result of a communication disorder. This is why the work of speech therapists inserted in educational settings is not clear in explaining the guidelines for therapeutic actions that favor the prevention of this phenomenon. The purpose of this research is to know the main conceptions that speech-language pathologists have about bullying in people with communication disorders at school age. With this objective, an investigation was carried out with quantitative and qualitative methods, mediated by surveys and in-depth interviews, respectively. It was found that more than 65% of bullying between people within educational establishments is related to children between 9 and 12 years of age with communication disorders. Regarding the self-perception of the speech-language pathology role in the issue of prevention and/or intervention in bullying issues, the concepts of favoring aspects of language and/or speech in children who have difficulty emerge, raising awareness of the actors involved, the reinforcement of self-esteem and interdisciplinary teamwork. Completing the analytical treatment of the interviews, it is established that speech therapy would play a fundamental role in addressing bullying in populations with communication disorders, being the professional who acts directly on communicative health.

Development of a multi-species SNP array for serrasalmid fish Colossoma macropomum and Piaractus mesopotamicus.

Scarce genomic resources have limited the development of breeding programs for serrasalmid fish Colossoma macropomum (tambaqui) and Piaractus mesopotamicus (pacu), the key native freshwater fish species produced in South America. The main objectives of this study were to design a dense SNP array for this fish group and to validate its performance on farmed populations from several locations in South America. Using multiple approaches based on different populations of tambaqui and pacu, a final list of 29,575 and 29,612 putative SNPs was selected, respectively, to print an Axiom AFFYMETRIX (THERMOFISHER) SerraSNP array. After validation, 74.17% (n = 21,963) and 71.25% (n = 21,072) of SNPs were classified as polymorphic variants in pacu and tambaqui, respectively. Most of the SNPs segregated within each population ranging from 14,199 to 19,856 in pacu; and from 15,075 to 20,380 in tambaqui. Our results indicate high levels of genetic diversity and clustered samples according to their hatchery origin. The developed SerraSNP array represents a valuable genomic tool approaching in-depth genetic studies for these species.

Genome-scale comparative analysis for host resistance against sea lice between Atlantic salmon and rainbow trout.

Sea lice (Caligus rogercresseyi) is an ectoparasite which causes major production losses in the salmon aquaculture industry worldwide. Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss) are two of the most susceptible salmonid species to sea lice infestation. The objectives of this study were to: (1) identify genomic regions associated with resistance to Caligus rogercresseyi in Atlantic salmon and rainbow trout by performing single-step Genome-Wide Association studies (ssGWAS), and (2) identify candidate genes related to trait variation based on exploring orthologous genes within the associated regions across species. A total of 2626 Atlantic salmon and 2643 rainbow trout were challenged and genotyped with 50 K and 57 K SNP panels, respectively. We ran two independent ssGWAS for sea lice resistance on each species and identified 7 and 13 regions explaining more than 1% of the genetic variance for the trait, with the most important regions explaining 3% and 2.7% for Atlantic salmon and rainbow trout, respectively. We identified genes associated with immune response, cytoskeleton function, and cell migration when focusing on important genomic regions for each species. Moreover, we found 15 common orthogroups which were present in more than one associated genomic region, within- or between-species; however, only one orthogroup showed a clear potential biological relevance in the response against sea lice. For instance, dual-specificity protein phosphatase 10-like (dusp10) and dual-specificity protein phosphatase 8 (dusp8) were found in genomic regions associated with lice density in Atlantic salmon and rainbow trout, respectively. Dusp10 and dusp8 are modulators of the MAPK pathway and might be involved in the differences of the inflammation response between lice resistant and susceptible fish from both species. Our results provide further knowledge on candidate genes related to sea lice resistance and may help establish better control for sea lice in fish populations.

A Morphological and Morphometric Dental Analysis as a Forensic Tool to Identify the Iberian Wolf (Canis Lupus Signatus).

Depredation by the Iberian wolf (Canis lupus signatus) is currently thought to be a problem in some areas of Spain. However, there are few technically validated forensic tools available to determine the veracity of claims with a high degree of scientific confidence, which is important given that such attacks may lead to compensation. The analysis of bite marks on attacked animals could provide scientific evidence to help identify the offender. Thus, the aim of this study was to assess the morphological and morphometric characteristics of Iberian wolf dentition. This data collection would serve as a base-point for a more accurate identification of the wolves thorough their bite marks. For the first time, 36 dental variables have been studied in wolves' skulls, employing univariate and multivariate analyses. The general morphological dental characteristics of wolves are very similar in terms of their dental formula and tooth structure to other canids, like domestic dogs. Sex differentiation was evident, principally in terms of the maxillary distance between the palatal surfaces of the canine teeth (UbC) and the width of the left mandibular canine teeth (LlCWd). New morphometric reference information was obtained that can aid the forensic identification of bite marks caused by the Iberian wolf with greater confidence.

Estimates of Autozygosity Through Runs of Homozygosity in Farmed Coho Salmon.

The characterization of runs of homozygosity (ROH), using high-density single nucleotide polymorphisms (SNPs) allows inferences to be made about the past demographic history of animal populations and the genomic ROH has become a common approach to characterize the inbreeding. We aimed to analyze and characterize ROH patterns and compare different genomic and pedigree-based methods to estimate the inbreeding coefficient in two pure lines (POP A and B) and one recently admixed line (POP C) of coho salmon (Oncorhynchus kisutch) breeding nuclei, genotyped using a 200 K Affymetrix Axiom® myDesign Custom SNP Array. A large number and greater mean length of ROH were found for the two "pure" lines and the recently admixed line (POP C) showed the lowest number and smaller mean length of ROH. The ROH analysis for different length classes suggests that all three coho salmon lines the genome is largely composed of a high number of short segments (<4 Mb), and for POP C no segment >16 Mb was found. A high variable number of ROH, mean length and inbreeding values across chromosomes; positively the consequence of artificial selection. Pedigree-based inbreeding values tended to underestimate genomic-based inbreeding levels, which in turn varied depending on the method used for estimation. The high positive correlations between different genomic-based inbreeding coefficients suggest that they are consistent and may be more accurate than pedigree-based methods, given that they capture information from past and more recent demographic events, even when there are no pedigree records available.

Estimación de edad mediante la relación área pulpa/diente en caninos mandibulares: estudio en una muestra chilena utilizando el Método de Cameriere / Dental age estimation by pulp/tooth ratio in mandibular canines: study in a Chilean sample by Camerierer's Method

La estimación de edad compone un aspecto importante en investigaciones forenses. Diferentes métodos se han descrito en odontología forense basadas en la correlación entre la edad y estructuras dentales. Cameriere et al. proponen un método cuantitativo para estimación de edad en adultos, a partir de la evaluación de la relación del área pulpa/diente, en base a la aposición de dentina secundaria. El objetivo del estudio fue desarrollar modelos de regresión para la estimación de edad dental mediante la relación área pulpa/diente en caninos inferiores en una muestra Chilena. Se analizaron 212 radiografías periapicales digitales (RPD) (86 hombres y 126 mujeres) de caninos mandibulares mediante el programa Image J para establecer el área de la pulpa y el diente. Se registraron los datos de sexo y edad de las RPD seleccionadas en forma ciega. Fueron desarrollados modelos de regresión lineal simples para la estimación de edad. El coeficiente de determinación para R33 fue 27,8 % y de 29,6 % para R44, con un error absoluto medio de 11,02 años y 10,37 años respectivamente. El análisis de ANOVA no mostró diferencias estadísticamente significativas para las relaciones área pulpa/diente de caninos según sexo (p> 0,05). Según los resultados obtenidos, la metodología propuesta por Cameriere et al. es fiable para estimar la edad dental mediante la relación área pulpa/diente en adultos. Sin embargo, en los modelos de regresión desarrollados para la población Chilena, se puede afirmar que el ajuste indicado por los coeficientes de determinación muestran incerteza entre las variables área pulpa/diente y edad cronológica en caninos inferiores, por lo tanto se sugiere considerar otros métodos adicionales para estimar edad en esta población.

Age estimation is an important aspect In forensic investigations. Different methods in forensic odontology based on the correlation between age estimation in adults, from the analysis of the pulp/tooth area, based on the apposition of secondary dentine. The aim of the study was to develop regression models for the dental age estimation by the relation pulp/tooth area, in lower canines in a Chilean sample, using digital peri-apical radiographs (DPR) applying Cameriere's method. We analyzed 212 DPR (86 males and 126 females) mandibular canines through Image J program to measure the pulp/tooth area. Age and sex information was obtained of the DPR's blindly selected. We developed simple linear regression models for age estimation. The coefficient of determination to R33 was R2 age and dental structures have been described. Cameriere et al. proposed a quantitative method for 27.8 % and R2 29.6 % to R44, with a mean absolute error of 11.02 years, to R33 and 10.37 years to R44. ANOVA analysis showed no statistically significant differences for the pulp/tooth relation area of canines according to sex (p> 0.05). According to the results, the Cameriere's et al., method is reliable for dental age estimation according to pulp/tooth ratio in adults. However, in the regression models developed for Chilean population, it can be stated that the adjustment indicated by the coefficients of determination, show uncertainty between the pulp / tooth area and chronological age in lower canines, therefore it is suggested to use additional estimation methods for age in this population.

Efecto de factores contextuales en la composición corporal de jugadores profesionales de fútbol. Un estudio retrospectivo / Effect of contextual factors on body composition in professional soccer players. A retrospective study

La exigencia de las demandas físicas en el fútbol ha evolucionado en los últimos años, poniendo de manifiesto la necesidad de investigar sobre aquellos aspectos que condicionan el rendimiento deportivo. Es por esto que el objetivo de este estudio fue describir la incidencia del entrenamiento individualizado, la compañía en las comidas, la raza y la demarcación sobre las variables antropométricas en jugadores de fútbol profesional. Para ello se desarrolló un estudio retrospectivo sobre 51 jugadores profesionales de la Segunda División B española durante las temporadas de 2015/2016, 2016/2017 y 2017/2018. La valoración antropométrica se realizó bajo las normas técnicas de medición recomendadas por el International Working Group of Kinanthropometry, adoptadas por la International Society for the Advancement of Kinanthropometry (ISAK). Los resultados revelaron que el entrenamiento individualizado y la compañía en las comidas fueron los factores que más influyeron sobre las variables antropométricas. Los valores de masa grasa y de masa muscular, y el sumatorio de pliegues son sensibles al efecto de la intervención sobre dichos factores. Los mayores niveles de interacción se producen entre la compañía en las comidas y el entrenamiento individualizado, y entre la demarcación y la compañía en las comidas. Considerando la composición corporal como un aspecto a tener en cuenta en el desarrollo del rendimiento, se concluye que la aplicación de ciertos contenidos del entrenamiento según las características individuales y el estilo de vida de los jugadores es un factor que posee una influencia significativa sobre los futbolistas profesionales

The requirements of physical demands in soccer have evolved in recent years, determining the need to investigate those aspects that condition athletic performance. The objective of this study was to describe the incidence of individualized training, company at meals, race, and demarcation on the anthropometric variables of professional soccer players since these four factors affect body composition, which is considered a predictor of performance and an indicator of lifestyle in these individuals. For this purpose, a retrospective study was developed in 51 professional players of the Spanish Football League Second Division B during the 2015/2016, 2016/2017, and 2017/2018 seasons. The anthropometric assessment was carried out under the technical standards of measurement recommended by the International Working Group of Kinanthropometry, adopted by the International Society for the Advancement of Kinanthropometry (ISAK). The results revealed that individualized training and company during meals were the factors that most influence exerted on the anthropometric variables that were collected. The values of fat mass and muscle mass, and the sum of fold measurements are sensitive to the effect of the intervention with these factors. The highest levels of interaction occurred between company during the meals and individualized training, and between demarcation and company during the meals. Considering body composition as an aspect to be taken into account in the development of performance, it should be considered that the application of certain training contents according to the individual characteristics and lifestyle of players are factors that may have a significant influence on professional soccer players

[Effect of contextual factors in body composition of professional soccer players. A retrospective study]. / Efecto de factores contextuales en la composición corporal de jugadores profesionales de fútbol. Un estudio retrospectivo.

INTRODUCTION: The requirements of physical demands in soccer have evolved in recent years, determining the need to investigate those aspects that condition athletic performance. The objective of this study was to describe the incidence of individualized training, company at meals, race, and demarcation on the anthropometric variables of professional soccer players since these four factors affect body composition, which is considered a predictor of performance and an indicator of lifestyle in these individuals. For this purpose, a retrospective study was developed in 51 professional players of the Spanish Football League Second Division B during the 2015/2016, 2016/2017, and 2017/2018 seasons. The anthropometric assessment was carried out under the technical standards of measurement recommended by the International Working Group of Kinanthropometry, adopted by the International Society for the Advancement of Kinanthropometry (ISAK). The results revealed that individualized training and company during meals were the factors that most influence exerted on the anthropometric variables that were collected. The values of fat mass and muscle mass, and the sum of fold measurements are sensitive to the effect of the intervention with these factors. The highest levels of interaction occurred between company during the meals and individualized training, and between demarcation and company during the meals. Considering body composition as an aspect to be taken into account in the development of performance, it should be considered that the application of certain training contents according to the individual characteristics and lifestyle of players are factors that may have a significant influence on professional soccer players.

INTRODUCCIÓN: La exigencia de las demandas físicas en el fútbol ha evolucionado en los últimos años, poniendo de manifiesto la necesidad de investigar sobre aquellos aspectos que condicionan el rendimiento deportivo. Es por esto que el objetivo de este estudio fue describir la incidencia del entrenamiento individualizado, la compañía en las comidas, la raza y la demarcación sobre las variables antropométricas en jugadores de fútbol profesional. Para ello se desarrolló un estudio retrospectivo sobre 51 jugadores profesionales de la Segunda División B española durante las temporadas de 2015/2016, 2016/2017 y 2017/2018. La valoración antropométrica se realizó bajo las normas técnicas de medición recomendadas por el International Working Group of Kinanthropometry, adoptadas por la International Society for the Advancement of Kinanthropometry (ISAK). Los resultados revelaron que el entrenamiento individualizado y la compañía en las comidas fueron los factores que más influyeron sobre las variables antropométricas. Los valores de masa grasa y de masa muscular, y el sumatorio de pliegues son sensibles al efecto de la intervención sobre dichos factores. Los mayores niveles de interacción se producen entre la compañía en las comidas y el entrenamiento individualizado, y entre la demarcación y la compañía en las comidas. Considerando la composición corporal como un aspecto a tener en cuenta en el desarrollo del rendimiento, se concluye que la aplicación de ciertos contenidos del entrenamiento según las características individuales y el estilo de vida de los jugadores es un factor que posee una influencia significativa sobre los futbolistas profesionales.

Single-Step Genome-Wide Association Study for Resistance to Piscirickettsia salmonis in Rainbow Trout (Oncorhynchus mykiss).

One of the main pathogens affecting rainbow trout (Oncorhynchus mykiss) farming is the facultative intracellular bacteria Piscirickettsia salmonis Current treatments, such as antibiotics and vaccines, have not had the expected effectiveness in field conditions. Genetic improvement by means of selection for resistance is proposed as a viable alternative for control. Genomic information can be used to identify the genomic regions associated with resistance and enhance the genetic evaluation methods to speed up the genetic improvement for the trait. The objectives of this study were to i) identify the genomic regions associated with resistance to P. salmonis; and ii) identify candidate genes associated with the trait in rainbow trout. We experimentally challenged 2,130 rainbow trout with P. salmonis and genotyped them with a 57 K single nucleotide polymorphism (SNP) array. Resistance to P. salmonis was defined as time to death (TD) and as binary survival (BS). Significant heritabilities were estimated for TD and BS (0.48 ± 0.04 and 0.34 ± 0.04, respectively). A total of 2,047 fish and 26,068 SNPs passed quality control for samples and genotypes. Using a single-step genome wide association analysis (ssGWAS) we identified four genomic regions explaining over 1% of the genetic variance for TD and three for BS. Interestingly, the same genomic region located on Omy27 was found to explain the highest proportion of genetic variance for both traits (2.4 and 1.5% for TD and BS, respectively). The identified SNP in this region is located within an exon of a gene related with actin cytoskeletal organization, a protein exploited by P. salmonis during infection. Other important candidate genes identified are related with innate immune response and oxidative stress. The moderate heritability values estimated in the present study show it is possible to improve resistance to P. salmonis through artificial selection in the rainbow trout population studied here. Furthermore, our results suggest a polygenic genetic architecture for the trait and provide novel insights into the candidate genes underpinning resistance to P. salmonis in O. mykiss.

Estimación de la edad dental en adultos mediante análisis de relación de área pulpa/diente en premolares y caninos mandi bulares mediante ortopantomografías, en una muestra chilena / Dental age estimation in adults by pulp/tooth ratio in lower premolars and canines by orthopantomographs, in a Chilean sample

La estimación de la edad es uno de los procedimientos más importantes para la identificación humana. Cameriere et al. propusieron un método para estimación de edad evaluando la relación área pulpa/diente utilizando radiografías dentales, basado en la aposición de dentina secundaria. El objetivo de este estudio, fue desarrollar modelos de regresión lineal para la estimación de la edad, mediante la relación de área pulpa/diente en caninos y premolares mandibulares utilizando ortopantomografías (OPGs) en una población chilena de individuos adultos de edad y sexo conocidos. Se analizaron 162 OPGs digitales (67 hombres y 95 mujeres) mediante el programa ImageJ, con el fin de medir las áreas señaladas. Mediante análisis de regresión, se estudiaron las posibles interacciones entre la edad, sexo y relación de área pulpa/diente. Esta relación disminuyó regularmente con la edad y varió de 0,03 a 0,6 para premolares y 0,04 a 0,6 para caninos respectivamente. El mejor modelo de estimación de edad fue para la relación del canino inferior izquierdo obteniendo un coeficiente de determinación de R2=8,7 %. La media de error absoluto, con un intervalo de confianza del 95 %, osciló entre 5,6 y 5,72 años. El análisis de ANCOVA no mostró diferencias significativas entre hombres y mujeres para la estimación de edad, al igual que para ambos lados de la cavidad oral. Según los resultados obtenidos, la relación de área pulpa/diente es una metodología fiable para la estimación de la edad en adultos. Sin embargo, los resultados demuestran que, en casos reales, es necesario contar con métodos adicionales de estimación ya que, al menos en la muestra analizada, el ajuste indicado por los coeficientes de determinación demostró falta de certeza en las estimaciones etarias producidas por los modelos de regresión lineal.

Age estimation is one of the most important procedures for human identification. Cameriere et al. proposed a method for age estimation based on the analysis of the pulp/tooth ratio by using dental x-rays, based on the apposition of secondary dentine. The objective of this study was to develop linear regression models for age estimation, by means of the pulp/tooth ratio of mandibular canines and premolars using orthopantomographies (OPGs) in a Chilean population of adults of known age and sex. We analyzed 162 digital OPGs (67 men and 95 women) through ImageJ software, in order to measure these areas. By means of regression analysis, the possible interactions between age, sex and pulp / tooth ratio were studied. This relationship decreased regularly with age and ranged from 0.03 to 0.6 for premolars and 0.04 to 0.6 for canines, respectively. The best model of age estimation was for the relation of the lower left canine, obtaining a coefficient of determination of R2=8.7 %. The mean absolute error, with a confidence interval of 95 %, ranged between 5.6 and 5.72 years. The ANCOVA analysis showed no significant differences between men and women for age estimation, as well as for both sides of the oral cavity. According to the results, the pulp/tooth ratio is a reliable methodology for estimating age in adults. However, the results showed that, in actual cases, it is necessary to use additional estimation methods since, at least in the sample analyzed, the adjustment indicated by coefficients of determination showed uncertainty in age estimation produced by the linear regression models.

Attitudes, Perceptions, and Preferences of Individuals from Temuco (Chile) About Denture Marking.

Denture marking (DM) helps identify edentulous people with different methods. Analyzing perceptions and attitudes of denture wearers would enable understanding of the practical aspects, an analysis still absent in South America. Fifty-three individual candidates for removable dentures were instructed on disaster victim identification (DVI) and the usefulness of DM for this purpose. They were physically shown eight DM systems and a questionnaire to which they responded by highlighting preferences in those systems. Although 98.11% did not know that dentures could be marked, only 9.43% denied interest in some type of DM. The 90.57% would mark their dentures with some system, preferring the inclusion of QR codes or a micro SIM card. The proven weaknesses of DVI and propensity for disaster mean this community in Chile is an opportunity to implement DM systems. Obstacles may come not from patients but from their dentists, educational institutions, or a lack of public policies.

Development and characterization of microsatellite markers in Gaultheria pumila Lf. (Ericaceae).

BACKGROUND: Polymorphic microsatellite markers were developed for Gaultheria pumila (Ericaceae) to evaluate genetic diversity and population structure within its native range in Chile. This is a very important Ericaceae endemic to Chile with a large commercial potential. Its resistance to different abiotic conditions makes it a valuable target for genetic improvement. RESULTS: Ten polymorphic simple sequence repeat (SSR) loci were isolated from Gaultheria pumila using new-generation 454 FLX Titanium pyrosequencing technology. The mean number of alleles per locus ranged from 2 to 4. Observed and expected heterozygosity ranged from 0.00 to 1.0 and 0.00 to 0.64, respectively. CONCLUSIONS: From 10 SSR markers developed for G. pumila, 9 markers are promising candidates for analyzing genetic variation within or between natural populations of G. pumila and other species from the same genus.

Vaccination-induced skin-resident memory CD8+ T cells mediate strong protection against cutaneous melanoma.

Memory CD8+ T cell responses have the potential to mediate long-lasting protection against cancers. Resident memory CD8+ T (Trm) cells stably reside in non-lymphoid tissues and mediate superior innate and adaptive immunity against pathogens. Emerging evidence indicates that Trm cells develop in human solid cancers and play a key role in controlling tumor growth. However, the specific contribution of Trm cells to anti-tumor immunity is incompletely understood. Moreover, clinically applicable vaccination strategies that efficiently establish Trm cell responses remain largely unexplored and are expected to strongly protect against tumors. Here we demonstrated that a single intradermal administration of gene- or protein-based vaccines efficiently induces specific Trm cell responses against models of tumor-specific and self-antigens, which accumulated in vaccinated and distant non-vaccinated skin. Vaccination-induced Trm cells were largely resistant to in vivo intravascular staining and antibody-dependent depletion. Intradermal, but not intraperitoneal vaccination, generated memory precursors expressing skin-homing molecules in circulation and Trm cells in skin. Interestingly, vaccination-induced Trm cell responses strongly suppressed the growth of B16F10 melanoma, independently of circulating memory CD8+ T cells, and were able to infiltrate tumors. This work highlights the therapeutic potential of vaccination-induced Trm cell responses to achieve potent protection against skin malignancies.

Adolescents with high intellectual ability: differences in body composition and physical activity by sex.

OBJECTIVES: Physical activity (PA) has been shown to have multiple health and wellness benefits, but there is no such information for adolescents with high intellectual ability (HIA). Thus, the aim of this study is to assess body composition and PA in HIA Chilean adolescents. METHODS: Weight and body composition were measured by bioelectrical impedance in 73 adolescents (39 female) aged 14-18 years from the Valparaíso region of Chile. HIA was assessed via Raven's Progressive Matrices (> 75th percentile) and PA, via questionnaire. Obesity was defined as a body fat percentage (BF%) ≥ 25 (for boys) or ≥ 30 BF% (for girls). RESULTS: Obesity prevalence was 43.59% in females and 8.82% in males. A total of 69% of adolescents performed more than two hours of weekly exercise, with the amount being greater in males. BF% and fat mass index were significantly different (p < 0.05) in adolescents who engaged in fewer than two hours of weekly exercise. On the contrary, subjects who performed more than two hours of weekly exercise exhibited higher mass muscle percentages (p < 0.01). After controlling for socioeconomic status and PA, the obesity odds ratio (OR = 7.6; 95% CI: 1.9-30.9) was significantly higher in females (p < 0.01). CONCLUSIONS: Adolescents with HIA reported elevated PA. However, obesity was more prevalent in females, who also reported less weekly PA than males.