RESUMEN
An increasing number of different clinical infections caused by Corynebacteria have been reported in the last decade. The aim of this study was to evaluate the antibiotic resistance rates, biofilm formation capacities and to investigate the ''anti-quorum-sensing (anti-QS)'' activities of corynebacteria, which were divided into three groups according to the type of growth in culture (pure, with another pathogenic bacterium and polymicrobial growth). In total 240 Corynebacterium spp. isolates from different clinical specimens sent to the medical microbiology laboratories of Düzce University Faculty of Medicine Hospital and Basaksehir Çam and Sakura City Hospital between June 2021 and June 2022 were classified into three groups: pure, isolated with another pathogen and polymicrobial, according to their growth patterns in culture. Bacteria were identified by matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) Biotyper (Bruker, Germany) at an external centre. Antibiotic susceptibilities were determined by disc diffusion method and for vancomycin broth microdilution method was used. Results were interpreted according to EUCAST recommendations. The biofilm-forming properties of the isolates were determined quantitatively. Bioactive components of 17 isolates with strong biofilm formation were extracted and anti-QS activity was determined by agar diffusion method using Chromobacterium violaceum ATCC 12472 strain and then violacein pigment production was measured quantitatively. Of the 240 Corynebacterium spp. isolates, 138 (58%) were pure, 52 (22%) were isolated with another pathogen and 50 (20%) were part of a polymicrobial infection. Of the isolates, 140 were identified as C.striatum, 34 as C.amycolatum and 24 as Corynebacterium afermentans. When the antibiotic resistance rates of the Corynebacterium isolates were analysed according to the groups, the resistance rates to rifampicin and tetracycline antibiotics were found to be statistically significantly lower in the polymicrobial group than in the other groups. The resistance rates to penicillin, clindamycin, ciprofloxacin, moxifloxacin, rifampicin, tetracycline and linezolid were 96.7%, 88.3%, 86.3%, 73.8%, 62.5%, 59.2% and 0.8%, respectively. While all isolates were susceptible to vancomycin, linezolid resistance was detected in two C.afermentans isolates. When the biofilm formation ability was analysed, it was observed that 87 (36.3%) isolates formed biofilm. The biofilm formation rate of the isolates in the polymicrobial growth group was lower than the other two groups. The anti-QS activity of 17 isolates with strong biofilm formation was investigated and none of the Corynebacterium extracts tested were found to have anti-QS activity (inhibition of violacein pigment production without inhibiting bacterial growth) in the QS study with C.violaceum, whereas five isolate extracts had antibacterial activity (inhibition of bacterial growth). Four of the bacterial extracts with antimicrobial activity belonged to C.amycolatum and one to C.afermentans. In conclusion, when both antibiotic resistance rates and biofilm formation rates were analysed, the corynebacteria growing in culture with another pathogen showed similar characteristics to the corynebacteria growing as a pure culture. Therefore, it was thought that corynebacteria growing with another pathogen should not be ignored. In addition, the antimicrobial effects of some corynebacterial extracts suggested that more QS studies should be carried out with microbiota bacteria.
Asunto(s)
Antibacterianos , Biopelículas , Infecciones por Corynebacterium , Corynebacterium , Pruebas de Sensibilidad Microbiana , Percepción de Quorum , Biopelículas/efectos de los fármacos , Biopelículas/crecimiento & desarrollo , Corynebacterium/efectos de los fármacos , Corynebacterium/aislamiento & purificación , Corynebacterium/crecimiento & desarrollo , Humanos , Antibacterianos/farmacología , Infecciones por Corynebacterium/microbiología , Percepción de Quorum/efectos de los fármacos , Farmacorresistencia Bacteriana , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción , Indoles/farmacologíaRESUMEN
OBJECTIVE: Because of the coronavirus disease 19 pandemic, studies on vaccination are being conducted in our country as well as across the world. In this study, the antibody levels in healthcare workers vaccinated with two doses of inactivated vaccine and the factors affecting these levels were investigated. METHODS: Randomly selected volunteers from healthcare workers, who had been vaccinated with two doses of inactivated vaccine in January to February 2021, were included in the study. Blood samples were drawn twice, 1 month and 6 months after the second dose vaccine (CoronaVac:Sinovac Life Science Co, Ltd, Beijing, China). The antibody levels were determined by the chemiluminescence microparticle immunoassay method using kits for quantitative detection of immunoglobulin class G antibodies to severe acute respiratory syndrome coronavirus 2. RESULTS: The mean antibody levels of 129 volunteers were 1232.5 (min: 103 to max: 7151) AU/mL in the first month and 403.5 (min: 23 to max: 4963) AU/mL in the sixth month. According to the survey results, 91 (71%) volunteers had not been diagnosed with coronavirus disease 19 before vaccination. The antibody levels 1 month and 6 months after the second dose of vaccination were significantly higher in those who had been diagnosed with coronavirus disease 19 before vaccination than in those who had not. It was found that age, gender, fast food, or healthy nutrition had no effect on antibody levels. CONCLUSION: Vaccines are very important both to protect against coronavirus disease 19 and to experience only a mild form of the disease. Immunoglobulin class G levels formed after vaccination may be affected by many factors and may decrease over time.
Asunto(s)
COVID-19 , Humanos , COVID-19/prevención & control , Vacunación , Personal de Salud , Isotipos de Inmunoglobulinas , Vacunas de Productos Inactivados , Anticuerpos AntiviralesRESUMEN
The use of tigecycline is becoming increasingly important because of the high levels of antibiotic resistance in Acinetobacter baumannii (A. baumannii) isolates. In this prospective study, multidrug-resistant A. baumannii isolates were obtained from various tissue and fluid samples of patients admitted to or treated at various departments and tested in Laboratory of Microbiology, Duzce University Medical Faculty between January 2013 and December 2015. Tigecycline resistance in multidrug-resistant A. baumannii isolates were analyzed using the disc diffusion test (DDT), agar gradient test (AGT), and gold standard test [broth microdilution test (BMT)]. A. baumannii isolates resistant to multiple drugs were included in the study (N=94). Using the BMT method, 89 (95%), 4 (4%) and 1 (1%) A. baumannii isolates were determined as tigecycline susceptible, intermediate and resistant isolates, respectively. Using the Food and Drug Administration criteria, the rates of major error (ME), minor error (mE) and categorical agreement (CA) for DDT were 26%, 67% and 9%, respectively. In contrast, for AGT, the rates of ME, mE and CA were 0%, 4%, 95%, respectively. Tigecycline resistance as assessed by BMT showed no increase between 2013 and 2015. Accordingly, isolates found to be resistant or intermediate by DDT should be confirmed by BMT. Due to the ease of application, AGT is a safe method of detecting susceptibility.
Asunto(s)
Infecciones por Acinetobacter , Acinetobacter baumannii , Humanos , Infecciones por Acinetobacter/tratamiento farmacológico , Agar/uso terapéutico , Antibacterianos/farmacología , Antibacterianos/uso terapéutico , Pruebas de Sensibilidad Microbiana , Minociclina/farmacología , Minociclina/uso terapéutico , Estudios Prospectivos , Tigeciclina/farmacología , Estados UnidosRESUMEN
BACKGROUND: Patients who come to the emergency pandemic outpatient clinic with a pre-diagnosis of COVID-19 are still a burden on the health system. Rapid triage of patients is important to reduce transmission. The aim of this study is to evaluate the biochemistry and hemogram results of real-time reverse transcription-polymerase chain reaction (RT-PCR) positive and negative patients in the emergency pandemic outpatient clinic and to investigate predictive values of the initial tests that will help to make rapid diagnosis. METHODS: Patients who applied to the emergency pandemic outpatient clinic with the suspicion of COVID-19 between November 01, 2020 and January 01, 2021 were evaluated with RT-PCR and laboratory examinations. RESULTS: A total of 551 patients were included in the study. The mean age of the patients was 50.31 ± 18.47 (min. 18 - max. 94), and 47.2% (n = 260) of the patients included in the study were male and 52.8% (n = 291) were female. In the comparison of hemogram parameters, we found that mean platelet volume (MPV) was significantly higher (p = 0.023), whereas white blood cell (WBC), platelet counts (PLT), lymphocyte and neutrophil values were significantly lower in RT-PCR positive patients (p < 0.001). There were no significant differences between the PCR positive and negative patients in terms of other parameters. In the comparison of biochemical parameters, we found that lactate dehydrogenase LDH (p = 0.001), creatinine (p = 0.002), and AST (p < 0.001) values were significantly higher in PCR positive patients, while there were no significant differences in terms of other biochemical parameters (p > 0.05). CONCLUSIONS: Our study results show that the practical quick-look hemogram and MPV can be used as a specially evaluated parameter in the rapid management of the first application COVID-19 patients. In addition, biochemically high levels of LDH and creatinine can be used to guide the clinician in terms of early hydration of the patient with a pre-diagnosis of COVID-19 to alleviate acute kidney damage.
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COVID-19 , Instituciones de Atención Ambulatoria , COVID-19/diagnóstico , Femenino , Humanos , Masculino , Pandemias , Reacción en Cadena en Tiempo Real de la Polimerasa , Estudios Retrospectivos , SARS-CoV-2 , TriajeRESUMEN
Objective: Nontuberculous mycobacteria (NTM) have been recognized as a diverse group of organisms that are ubiquitous in environmental sources. In most regions of the world, NTM are not reportable as a public health disease, so epidemiological data are not easily available. However, data in published studies note increasing trends at the rate of NTM isolation from different geographic regions of the world. Increasing NTM isolation may have important public health implications. The aim of our study is the investigation of NTM from water resources and networks in Düzce, Turkey. Methods: NTM are common in water resources and water networks. They can cause waterborne infections in humans. A total of 120 water samples measured of chlorine and pH levels were decontaminated and filtered. Then, the filters were placed in the culturing media. Statistical Analysis Used: Chi-square and t-test were used for the statistical analysis. Results: NTM were detected in 20 (16.6%) samples. Nine of them (45%) were Mycobacterium fortuitum, three (15%) were Mycobacterium gordonae, three (15%) were Mycobacterium szulgai, two (10%) were Mycobacterium lentiflavum, two (10%) were Mycobacterium chelonae, and one (5%) was Mycobacterium peregrinum. Conclusions: These environmental bacteria can cause serious illnesses in both immunocompetent and especially immunocompromised individuals. For the correct treatment of these patients, it is important to determine NTM in clinical samples. Surveillance is necessary to know the source of NTM infection, to identify and type the strains, and to establish effective control measures such as disinfection, maintenance, and modernization of water systems.
Asunto(s)
Agua Potable , Infecciones por Mycobacterium no Tuberculosas , Mycobacterium , Humanos , Mycobacteriaceae , Infecciones por Mycobacterium no Tuberculosas/epidemiología , Micobacterias no TuberculosasRESUMEN
BACKGROUND: The coronavirus disease 2019 (COVID-19) outbreak has caused great difficulties in providing healthcare services worldwide. Accurate and useful indicators are needed for the effective use of resources. Carbon monoxide (CO) is also produced endogenously without exogenous exposure. Studies confirm that endogenous CO increases in conditions such as pneumonia, sepsis, and trauma. This study aimed to compare patients diagnosed with COVID-19 pneumonia and healthy people to reveal whether endogenous carboxyhemoglobin (COHb) levels differ. It was also to determine whether it could be a helpful indicator for the clinician in predicting disease severity in combating the COVID-19 pandemic. METHODS: The study included 72 patients admitted to the hospital during the COVID-19 pandemic and diagnosed with COVID-19 pneumonia and a control group of 75 healthy people. The research data was collected retrospectively in a tertiary emergency department. The patients' demographic data, pneumonia severity index (PSI) score, CURB-65 score, 30-day mortality, hospitalization, need for mechanical ventilation (MV), and COHb levels were recorded. RESULTS: While the COHb level was 0.9% (min-max, 0.1 - 4.0) in patients with COVID-19 pneumonia, it was 1% (min-max, 0.1 - 2.6) in the control group. No significant difference was found between the patients and the control group in terms of COHb levels (p = 0.341). Also, there was no correlation between COHb levels and PSI and CURB-65 scores. No significant difference was detected between COHb levels and patients diagnosed with COVID-19 pneumonia in terms of mortality, need for MV, or hospitalization (p > 0.05). CONCLUSIONS: We concluded that COHb levels alone could not be used to diagnose COVID-19 pneumonia or predict disease severity. Besides, in COVID-19 pneumonia, the COHb level was not a useful indicator of mortality, need for MV, or hospitalization decision. Further studies are needed to find useful indicators to assist clinicians in their decision-making process in tackling the COVID-19 pandemic.
Asunto(s)
COVID-19 , Neumonía , Carboxihemoglobina/análisis , Humanos , Pandemias , Neumonía/diagnóstico , Pronóstico , Estudios Retrospectivos , SARS-CoV-2RESUMEN
High-risk human papilloma virus (hrHPV) plays an important role in cervical cancer. The aim of this study was to investigate the distribution of HPV genotypes in the region and to correlate it with liquid-based-cytology (LBC) and colposcopic biopsy results. Furthermore, the potential relationship between HPV infections and bacterial vaginosis (BV) was investigated. HPV genotypes were determined using real-time PCR. LBC, biopsies, and BV examinations were performed by the Pathology and Cytology. Consecutive cervical specimens of 409 women who underwent both cytology and HPV-DNA tests were included in the study. A total of 172 (42.1%) patients were positive for HPV-DNA; of these, 107 (26.2%) had hrHPV. The most common HPV genotypes were HPV 59, 16, 33, 52, and 51, at 16.6%, 15.9%, 13.4%, 13.4%, and 8.9%, respectively. Epithelial cell abnormality was detected in 11.5% of LBC test results. The genotypes of HPV 33, 56, 66, and 68 were found at a higher rate in patients with epithelial cell abnormalities than in those with no detected abnormalities. Bacterial vaginosis was found in 24 patients (5.9%). HPV-DNA positivity was observed to be statistically higher in patients with BV than in those without BV.
Asunto(s)
Genotipo , Papillomaviridae/genética , Infecciones por Papillomavirus/virología , Adulto , Biopsia/métodos , Colposcopía/métodos , ADN Viral/análisis , Femenino , Humanos , Persona de Mediana Edad , Prueba de Papanicolaou/métodos , Infecciones por Papillomavirus/patología , Centros de Atención Terciaria , Turquía , Neoplasias del Cuello Uterino/epidemiología , Neoplasias del Cuello Uterino/virología , Vaginosis Bacteriana/epidemiología , Vaginosis Bacteriana/virologíaRESUMEN
BACKGROUND: Stenotrophomonas maltophilia is a Gram-negative bacillus and opportunistic emergent pathogen causing hospital-acquired infections (HAIs). Due to risk factors such as prolonged intensive care unit stay and invasive procedures, it has become one of the leading causes of HAIs. OBJECTIVE: The aim of this study was to evaluate the epidemiology of S.maltophilia infections over a six-year period at Düzce University Hospital, Turkey. METHODS: The incidence, clinical characteristics, antimicrobial susceptibility and outcomes of nosocomial S. maltophilia infections during this period were retrospectively analyzed. RESULTS: During the study period, 67 samples obtained from 61 patients were identified. Pneumonias (82%) were the most common HAIs, followed by bloodstream infections (10.5%), urinary tract infections (3%), skin and soft tissue infections (3%) and surgical site infection (1.5%). Admission to intensive care, hospitalization exceeding 30 days, and previous use of broad-spectrum antibiotics constituted risk factors. Resistance to cotrimoxazole (6%) was lower than that to levofloxacin (18%). CONCLUSION: The most important risk factors for S.maltophilia infection in patients are previous exposure to antibiotics, prolonged hospitalization and invasive procedures such as mechanic ventilation. Discharging patients as early as possible with the rational use of antibiotics may be effective in reducing S. maltophilia infections and resistance rates.
Asunto(s)
Infección Hospitalaria/microbiología , Infecciones por Bacterias Gramnegativas/diagnóstico , Hospitalización/estadística & datos numéricos , Stenotrophomonas maltophilia/aislamiento & purificación , Adolescente , Adulto , Distribución por Edad , Anciano , Anciano de 80 o más Años , Antibacterianos/uso terapéutico , Bacteriemia/diagnóstico , Bacteriemia/epidemiología , Bacteriemia/microbiología , Infección Hospitalaria/epidemiología , Femenino , Infecciones por Bacterias Gramnegativas/tratamiento farmacológico , Infecciones por Bacterias Gramnegativas/epidemiología , Infecciones por Bacterias Gramnegativas/microbiología , Humanos , Masculino , Persona de Mediana Edad , Estudios Retrospectivos , Factores de Riesgo , Stenotrophomonas maltophilia/efectos de los fármacos , Turquía/epidemiologíaRESUMEN
OBJECTIVE: To investigate the susceptibility and specificity of the phenotypic methods to determine plasmidmediated AmpC. METHODS: The cross-sectional study was conducted at Duzce University Faculty of Medicine, Microbiology Laboratory from January 2015 to June 2016, and comprised Escherichia coli and Klebsiella pneumonia isolates intermediate susceptible or resistant to cefoxitine. Combined disk diffusion test, double disc synergy test, agar gradient test and polymerase chain reaction were used to detect plasmid-mediated AmpC. RESULTS: Of the 2024 E. coli samples, 44(2.17%), and of the 792 K. pneumoniae samples, 16(2%) were included. Combined disk diffusion test had susceptibility of 68% and specificity of 50%; double disc synergy test 24% and 82%; and agar gradient test 40% and 68%. Of the isolates positively detected by polymerase chain reaction method, more than one gene region positivity was detected in 15(25%) isolates. CONCLUSION: All three phenotypic methods were found to be insufficient to detect plasmid-mediated AmpC positivity.
Asunto(s)
Proteínas Bacterianas/genética , ADN Bacteriano/genética , Escherichia coli , Klebsiella pneumoniae , Resistencia betalactámica/genética , beta-Lactamasas/genética , Adulto , Anciano , Antibacterianos/farmacología , Cefoxitina/farmacología , Estudios Transversales , Escherichia coli/efectos de los fármacos , Escherichia coli/genética , Infecciones por Escherichia coli/microbiología , Femenino , Humanos , Infecciones por Klebsiella/microbiología , Klebsiella pneumoniae/efectos de los fármacos , Klebsiella pneumoniae/genética , Masculino , Pruebas de Sensibilidad Microbiana , Persona de Mediana Edad , Plásmidos/genéticaRESUMEN
Background/aim: Hantavirus and Borrelia burgdorferi are two zoonotic agents that pose a great risk especially for people living in forest areas. This study aimed to investigate the seroprevalence of hantavirus and B. burgdorferi in forest villages of Düzce and its relationship with sociodemographic features. Materials and methods: The presence of immunoglobulin M (IgM) and immunoglobulin G (IgG) antibodies against hantavirus and B. burgdorferi in serum samples was investigated via enzyme-linked immunosorbent assay (ELISA). Hantavirus IgG and B. burgdorferi IgM and IgG positivity was then validated by western blot (WB) method. Results: During the analyses, 193 serum samples were tested. Eleven (6%) cases of hantavirus IgM was found positive by ELISA. Six (3%) cases of hantavirus IgG, 3 (2%) cases of B. burgdorferi IgM, and 12 (6%) cases of B. burgdorferi IgG were found positive by WB. Borrelia burgdorferi IgG positivity was found to be higher in the 4670 age group and it was seen that the positivity was the highest among those who lived in mud-brick houses. Conclusion: This study was the first to determine the frequency of hantavirus in the study region and it includes current data for B. burgdorferi. Consequently, it is recommended that similar studies be carried out on rodents in all the regions at risk.
Asunto(s)
Borrelia burgdorferi/aislamiento & purificación , Bosques , Infecciones por Hantavirus/epidemiología , Infecciones por Hantavirus/virología , Enfermedad de Lyme/epidemiología , Enfermedad de Lyme/microbiología , Orthohantavirus/aislamiento & purificación , Adulto , Anciano , Anticuerpos Antibacterianos/sangre , Western Blotting , Borrelia burgdorferi/inmunología , Ensayo de Inmunoadsorción Enzimática , Femenino , Orthohantavirus/inmunología , Infecciones por Hantavirus/sangre , Humanos , Inmunoglobulina G/sangre , Inmunoglobulina M/sangre , Enfermedad de Lyme/sangre , Masculino , Persona de Mediana Edad , Estudios Seroepidemiológicos , Turquía/epidemiología , Adulto JovenRESUMEN
PURPOSE: To compare effects of 5% topical povidone iodine with prophylactic topical azithromycin and moxifloxacin on bacterial flora in patients undergoing intravitreal injection. METHODS: A total of 132 patients were randomly assigned to receive treatment with azithromycin or moxifloxacin, or no treatment (control group). In total, 528 specimens were obtained at the time of admission, 4 days before intravitreal injection, 4 days after intravitreal injection, and 8 days after intravitreal injection. Samples were immediately sent to the microbiology laboratory for incubation. RESULTS: The microorganism observed most frequently was coagulasenegative Staphylococcus (23.8%). When the results of samples obtained on Day 4 before injection were assessed, growth of coagulase-negative Staphylococcus was significantly lower in the moxifloxacin group, compared with controls (p=0.049). Acinetobacter baumannii continued to grow after administration of azithromycin (p=0.033). When the results of four days after intravitreal injection were evaluated, growth of coagulase-ne gative Staphylococcus was higher in controls, compared with patients who received azithromycin or moxifloxacin (p=0.004). Eradication rate was significantly higher in the moxifloxacin group than in the control group (p=0.001). Samples obtained on Day 8 after intravitreal injection showed similar levels of bacterial growth in all groups (p=0.217). CONCLUSION: Moxifloxacin was more effective than 5% povidone iodine in controlling the growth of conjunctival bacterial flora. Use of moxifloxacin in combination with 5% povidone iodine resulted in a synergistic effect.
Asunto(s)
Antibacterianos/administración & dosificación , Antiinfecciosos Locales/administración & dosificación , Azitromicina/administración & dosificación , Conjuntiva/microbiología , Inyecciones Intravítreas/métodos , Moxifloxacino/administración & dosificación , Povidona Yodada/administración & dosificación , Acinetobacter/efectos de los fármacos , Acinetobacter/aislamiento & purificación , Conjuntiva/efectos de los fármacos , Conjuntivitis Bacteriana/microbiología , Conjuntivitis Bacteriana/prevención & control , Endoftalmitis/microbiología , Endoftalmitis/prevención & control , Escherichia coli/efectos de los fármacos , Escherichia coli/aislamiento & purificación , Humanos , Serratia marcescens/efectos de los fármacos , Serratia marcescens/aislamiento & purificación , Staphylococcus aureus/efectos de los fármacos , Staphylococcus aureus/aislamiento & purificación , Factores de Tiempo , Resultado del TratamientoRESUMEN
In recent years, the significant increase in the isolation of antifungal resistant Candida species in presence of biofilm, have made it necessary to develop alternative agents for the treatment of these infections. In this study, the effect of antifungal susceptibility of melatonin were investigated in planktonic and biofilm forms of Candida strains isolated from the clinical samples sent to our laboratory. Biofilm formation was determined by modified microplate method. In order to determine antifungal susceptibility in biofilm-forming strains, MIC was determined by broth microdilution method in planktonic form, and MBEC values by Calgary biofilm method in biofilm form. Susceptibility tests were repeated in the presence of melatonin. Antifungal susceptibility tests repeated with antifungals combined with melatonin showed a decrease in both MIC and MBEC values; melatonin was found to be more effective especially in planktonic forms. While the most effective combination was achieved with fluconazole in the planktonic form, no statistically significant difference was found between the combinations in biofilm form. As a result, melatonin was thought to be a new alternative in the treatment of Candida infections.
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Antifúngicos/farmacología , Biopelículas/efectos de los fármacos , Candida/efectos de los fármacos , Candidiasis/microbiología , Melatonina/farmacología , Biopelículas/crecimiento & desarrollo , Candida/crecimiento & desarrollo , Candida/aislamiento & purificación , Sinergismo Farmacológico , Humanos , Pruebas de Sensibilidad Microbiana , Viabilidad Microbiana/efectos de los fármacosRESUMEN
INTRODUCTION: The spread of Acinetobacter baumannii, resistant to most of the available antimicrobial agents, is a serious health problem. The high rate of carbapenem resistance among Acinetobacter baumannii isolates is considered as a threat to public health. In this study, we aimed to determine the antibiotic resistance and related genes in carbapenem-resistant Acinetobacter baumannii isolates. METHODOLOGY: Ninety six isolates of A. baumannii were included. Antimicrobial susceptibility was performed by Phoenix Automated System and disk diffusion method. Carbapenem resistane was characterized by scrneeing of resistance genes such as blaTEM, blaSHV, blaCTX-M1-2, blaPER, blaVEB, blaKPC, blaGES, blaNDM, blaVIM, blaIMP and blaOXA23-24-51-58 using multiplex polymerase chain reaction. RESULTS: Resistance for the levofloxacin, gentamicin, amikacin, and tigecycline were determined as 96.9%, 93.7%, 72.9% and 45.8% respectively. Colistin was the only susceptible antibiotic against all clinical isolates. All isolates were defined as multidrug resistance and of these, 31.2% were extensively drug-resistant (sensitive only to colistin). BlaOXA-51- and blaOXA-23 genes were detected in 100% strains while blaTEM was found in only 2% strains. There was no amplification for the blaSHV, blaCTX-M1-2, blaPER, blaVEB, blaKPC, blaGES blaNDM, blaVIM, blaIMP and blaOXA24-58 genes. CONCLUSIONS: The high frequency of blaOXA-23 and low frequency of blaTEM gene was observed that indicate prevalence of a variety of A. baumannii strains. The rates of resistance genes vary from region to region. Studies are required for the prevention and control of A. baumannii infection and to formulate the strategies of antibiotic usage.
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Infecciones por Acinetobacter/microbiología , Acinetobacter baumannii/efectos de los fármacos , Acinetobacter baumannii/genética , Carbapenémicos/farmacología , Resistencia betalactámica , beta-Lactamasas/genética , Acinetobacter baumannii/enzimología , Acinetobacter baumannii/aislamiento & purificación , Hospitales Universitarios , Humanos , Pruebas de Sensibilidad Microbiana , Reacción en Cadena de la Polimerasa Multiplex , TurquíaRESUMEN
Cutaneous larva migrans (CLM) is a parasitosis frequently seen in persons who have travelled to tropical or subtropical regions and in those who have worked in contact with soil. The disease frequently develops due to Ancylostoma braziliensis and Ancylostoma caninum species. After penetrating the skin and entering the body, the hookworm larva proceeds to bore tunnels through the epidermis, creating pruritic, erythematous, serpiginous lesions. Secondary bacterial infections of the lesions can often be seen, especially on the legs and buttocks. In this article we presented three atypical local cases which have not been declared previously in our country. The first case, a 54-year-old male who was admitted to hospital in August with complaints of an obverse body rash and itching lasting for a week. Eruptions were observed over a small area on the right side of the abdomen, consisting of itchy, raised, erythematous, curvilinear string-like lesions. Moreover, no eosinophilia was detected in the patient, whose culture showed a growth of Streptococcus pyogenes. The patient was clinically diagnosed with CLM accompanied by secondary bacterial infection and treated for three days with 1 g of amoxicillin-clavulanic acid, mupirocin cream and albendazole 400 mg/d. Under this regime, the lesions were seen to decline. The second case, a 38-year-old male was also admitted in August, complaining of itching and redness on his body. The patient, whose blood count values were normal, exhibited itchy, raised, serpiginous string-like lesions located on the left side of his body. The patient, whose bacterial culture was negative, was clinically diagnosed as CLM and treated for three days with albendazole 400 mg/d and the lesions were seen to improve. The third case, a 23-year old male was admitted in September complaining of itching and redness on his neck. An itchy, crescent-shaped erythematous lesion was detected on his neck; bacteriological cultures and blood count were normal. The common feature for all three cases was the story of working in a hazelnut orchard and mowing weeds using a motorized string trimmer (weed whacker). None of them had a history of travel outside the country. Therefore CLM assumed to be occurred due to the aeration of surface earth layer with the force of motorized string trimmer and entrance of the larvae were from the open parts of the body. In conclusion, it should be keep in mind that hookworm larva-related CLM can be encountered in our country, and reporting of the patients with similar findings are necessary to determine the prevalence of this parasitosis in our country.
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Enfermedades de los Trabajadores Agrícolas/parasitología , Larva Migrans/parasitología , Adulto , Enfermedades de los Trabajadores Agrícolas/tratamiento farmacológico , Enfermedades de los Trabajadores Agrícolas/microbiología , Albendazol/uso terapéutico , Combinación Amoxicilina-Clavulanato de Potasio/uso terapéutico , Antibacterianos/uso terapéutico , Antinematodos/uso terapéutico , Humanos , Larva Migrans/complicaciones , Larva Migrans/tratamiento farmacológico , Masculino , Persona de Mediana Edad , Mupirocina/uso terapéutico , Infecciones Estreptocócicas/complicaciones , Streptococcus pyogenes/aislamiento & purificación , Turquía , Adulto JovenRESUMEN
OBJECTIVE: In this study, the prevalence of intestinal parasites and its relationship with some hygiene habits and socio-demographic characteristics of students in Yigilca were investigated. METHODS: This study was conducted in three parts. Firstly, the prevalence of parasitic diseases was measured, and questionnaires about the level of knowledge of hygiene were administered. Secondly, control and intervention groups were determined. Lastly, some education and training courses were organized and provided to the intervention group, including parents and children, about the prevention of parasitic diseases and habits of cleaning. RESULTS: In this study, 540 cellophane tapes and 523 stool samples were studied. Among the samples analyzed, 87 (16.1%) with Enterobius vermicularis, 79 (15.1%) with Giardia intestinalis, 73 (13.9%) with various non-pathogenic parasites, 1 (0.19%) with Ascaris lumbricoides, and 1 (0.19%) with Hymenolepis nana were found. G. intestinalis was highly positive among the children who do not wash their hands after defecation. Also, some intestinal parasites were found to be related to the socio-demographic characteristics of families; parental education, family income, and father's profession were found to be associated with the presence of intestinal parasites. After training, the intervention group showed a significant decrease in the number of giardiasis and enterobiasis cases. CONCLUSION: Education on hygiene will prevent parasitic diseases from an early age.
Asunto(s)
Conocimientos, Actitudes y Práctica en Salud , Higiene/normas , Parasitosis Intestinales/epidemiología , Estudiantes , Adolescente , Animales , Ascaris lumbricoides/aislamiento & purificación , Niño , Enterobiasis/epidemiología , Enterobiasis/prevención & control , Enterobius/aislamiento & purificación , Heces/parasitología , Femenino , Giardia lamblia/aislamiento & purificación , Giardiasis/epidemiología , Giardiasis/prevención & control , Humanos , Higiene/educación , Hymenolepis nana/aislamiento & purificación , Parasitosis Intestinales/prevención & control , Masculino , Padres/educación , Prevalencia , Factores de Riesgo , Instituciones Académicas , Encuestas y Cuestionarios , Adulto JovenRESUMEN
Probiotics which are non-pathogenic live microorganisms ingested along with food or as dietary supplements, are thought to be beneficial to the host by supporting the microbial balance in digestive system. Various studies suggest that the effects of probiotics on the intestinal mucosa and immunity are protective against bacterial translocation. We aimed to investigate bacterial translocation related to the amount of CO2 insufflation given during laparoscopy and the effect of probiotic bacteria in an experimental peritonitis model. In this study 60 Wistar rats were used in six groups consisting of 10 rats. Group 1, 3 and 5 consisted of the rats that were fed without probiotics, while the rats in Group 2, 4, and 6 were fed with water containing 5 x 108 cfu/ml probiotic bacteria complex (Bifidobacterium lactis, Lactobacillus bulgaricus, Streptococcus thermophilus) for 15 days. To generate experimental peritonitis, 2 x 107 cfu/ml Escherichia coli ATCC 25922 was inoculated intraperitoneally to all of the rats. Thereafter, laparoscopy was applied in all groups. Application in Group 1 and Group 2 was without CO2; Group 3 and Group 4 with 14 mmHg CO2 insufflation, and Group 5 and Group 6 with 20 mmHg CO2 insufflation. Blood samples were taken in 2nd, 4th, and 6th hours. Mesenteric lymph node, liver and spleen samples were taken at 6th hour when the rats were sacrificed and then these were evaluated microbiologically with qualitative and quantitative methods. Bacterial translocation and bacteremia were found in the rats that were undergone experimental peritonitis during laparoscopy. All positive tissue and blood cultures yielded E.coli. The highest level of bacterial translocation was found to be in mesenteric lymph nodes (in 3/10, 6/10 and 10/10 in groups 1, 3 and 5 fed without probiotics, respectively; in 2/10, 3/10 and 4/10 in groups 2, 4 and 6 fed with probiotics, respectively). The bacterial translocation rates were found to be related to the increased CO2 insufflation. It was found that probiotic bacteria were more effective for decreasing bacterial translocation rates and bacteremia in the groups that were given high CO2 pressure during laparoscopy. It was also found that these results were correlated with bacterial translocation per gram of tissue. As an example, the quantitative bacterial growth values detected in mesenteric lymph node were 5.4 ± 2.9 x 103, 10.6 ± 3.3 x 103 and 21.5 ± 12.4 x 103 cfu/g in groups 1, 3 and 5, fed without probiotics, respectively; and 2.0 ± 1.3 x 103, 3.8 ± 1.9 x 103 and 9.0 ± 3.1 x 103 cfu/g in groups 2, 4 and 6, fed with probiotics, respectively. Our data emphasized that probiotic bacteria may be used as prophylactic agents for the prevention of bacterial translocation during laparoscopy, however comprehensive and clinical studies are needed to support these experimental results.