Brazil is recognized for its biodiversity and the genetic variability of its organisms. This genetic variability becomes even more valuable when it is properly documented and accessible. Understanding bacterial diversity through molecular characterization is necessary as it can improve patient treatment, reduce the length of hospital stays and the selection of resistant bacteria, and generate data for health and epidemiological surveillance. In this sense, in this study, we aimed to understand the biodiversity and molecular epidemiology of carbapenem-resistant bacteria in clinical samples recovered in the state of Rondônia, located in the Southwest Amazon region. Retrospective data from the Central Public Health Laboratories (LACEN/RO) between 2018 and 2021 were analysed using the Laboratory Environment Manager Platform (GAL). Seventy-two species with carbapenem resistance profiles were identified, of which 25 species carried at least one gene encoding carbapenemases of classes A (blaKPC-like), B (blaNDM-like, blaSPM-like or blaVIM-like) and D (blaOXA-23-like, blaOXA-24-like, blaOXA-48-like, blaOXA-58-like or blaOXA-143-like), among which we will highlight Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii, Serratia marcescens, and Providencia spp. With these results, we hope to contribute to the field by providing epidemiological molecular data for state surveillance on bacterial resistance and assisting in public policy decision-making.
Biodiversity , Carbapenems , beta-Lactamases , Brazil , Humans , Carbapenems/pharmacology , beta-Lactamases/genetics , Retrospective Studies , Anti-Bacterial Agents/pharmacology , Acinetobacter baumannii/genetics , Acinetobacter baumannii/drug effects , Acinetobacter baumannii/isolation & purification , Bacterial Proteins/genetics , Microbial Sensitivity Tests , Bacteria/genetics , Bacteria/drug effects , Bacteria/classification , Pseudomonas aeruginosa/genetics , Pseudomonas aeruginosa/drug effects , Pseudomonas aeruginosa/isolation & purification , Drug Resistance, Bacterial/genetics , Klebsiella pneumoniae/genetics , Klebsiella pneumoniae/drug effects , Klebsiella pneumoniae/isolation & purification
The objective in this study was to evaluate the effects of nitrogen rates in association with rates of the growth retardant trinexapac-ethyl on wheat. The experiment was conducted in Viçosa, MG and arranged in a 5×4 factorial, randomized block design, with four repetitions. A combination of five nitrogen rates (30, 60, 90, 120 and 150kg ha-1) with four rates of trinexapac-ethyl (0, 62.5, 125 and 187.5g ha-1) were tested. Trinexapac-ethyl promotes reduction of soot dry mass and grain yield at the lowest N rates, but at the highest N rates there is increase in these characteristics. The combination between N and trinexapac-ethyl rates that promotes higher shoot dry mass is 150kg ha-1 and 187g ha-1 but that promotes higher grain yield is 100kg ha-1 and 120g ha-1, respectively.
O objetivo neste estudo foi avaliar os efeitos de doses de nitrogênio associadas às doses do redutor de crescimento trinexapac-etil na cultura do trigo. O experimento, conduzido em Viçosa-MG, seguiu um esquema fatorial 5×4, no delineamento experimental de blocos casualizados, com quatro repetições. Os tratamentos foram 30, 60, 90, 120 e 150kg ha-1 de N, combinados com 0, 62,5, 125 e 187,5g ha-1 de trinexapac-etil. Trinexapac-etil promove redução da massa seca da parte aérea e do rendimento de grãos nas menores doses de N, mas sob doses elevadas de N o trinexapac-etil promove aumento dessas características. A combinação entre doses de N e trinexapac-etil que promove maior massa seca da parte aérea é de 150kg ha-1 e 187g ha-1, mas a que promove maior produção de grãos é de 100kg ha-1 e 120g ha-1, respectivamente.
