RESUMEN
DNA profiling can identify an individual from a sample of biological material but it does not reveal what body fluid or tissue source the DNA profile originated from. In many cases it is important to know from what body fluid or tissue the DNA profile originated in order to provide crucial information necessary to the investigation, especially in cases where the victims are not able to give information about the dynamics of the event. For this purpose messenger RNA (mRNA) analysis has been shown to be a suitable method for the identification of body fluids, resulting in a trend to overcome the conventional approaches. Here we present the first report about case regarding a three-year-old child supposedly victim of a sexual assault with digital penetration. Thanks to the use of the combined DNA profiling and RNA analysis it was possible to demonstrate the sexual assault suffered by the victim.
Asunto(s)
Abuso Sexual Infantil , Víctimas de Crimen , Dermatoglifia del ADN/métodos , Genética Forense/métodos , ARN Mensajero/análisis , Preescolar , Femenino , Humanos , UñasRESUMEN
Allele and genotype frequencies for the five loci LDLR, GYPA, HBGG, D7S8 and GC were determined for 374 unrelated Italians using a multiplex PCR-amplification and typing commercial kit. The distribution of the genotype frequencies showed no deviations from Hardy-Weinberg expectations. The combined power of discrimination and chance of exclusion for all five loci were 0.999 and 0.702, respectively. A test for homogeneity was performed and no significant differences were observed among the Caucasian population samples.
Asunto(s)
Alelos , Dermatoglifia del ADN , Etnicidad/genética , Frecuencia de los Genes , Genotipo , Humanos , Italia , Valores de ReferenciaRESUMEN
Orosomucoid subtypes have been analysed in 600 unrelated individuals from the Brescia Area (Lombardy-Italy) by the isoelectric focusing technique and immunoprinting. Five ORM1 phenotypes were observed. The ORM2 pattern was found to be virtually monomorphic, but one variant with an additional single band was found. The estimated allele frequencies were: ORM1*F1 = 0.5992, ORM1*F2 = 0.0150, ORM1*S = 0.3858.
Asunto(s)
Genética de Población , Orosomucoide/genética , Fenotipo , Polimorfismo Genético/genética , Alelos , Donantes de Sangre , Frecuencia de los Genes/genética , Humanos , Italia , Orosomucoide/clasificaciónRESUMEN
Alpha 2HS-subtypes have been analysed in samples from 700 unrelated individuals from the Brescia area (Italy) by the isoelectric focusing technique and immunofixation. The observed allele frequencies were: alpha 2HS*1 = 0.7472; alpha 2HS*2 = 0.2507; alpha 2HS*V = 0.0021.