Fermentation of whey-derived matrices by Kluyveromyces marxianus: alcoholic beverage development from whey and fruit juice mixes.

This research paper addresses the hypotheses that Kluyveromyces marxianus can be cultured with good alcohol production on different whey-derived matrices, and that the fermented product can be used in order to develop alcoholic beverages with acceptable sensory characteristics by mixtures with yeast-fermented fruit-based matrices. Growth and fermentative characteristics of Kluyveromyces marxianus LFIQK1 in different whey-derived matrices were explored by culturing (24 h, 30°C) on reconstituted whey, demineralized whey, heat-treated whey and milk permeate media. High lactose consumption, ethanol production and yield were observed. Reconstituted whey matrix was selected for mixing with orange or strawberry juices fermented using Saccharomyces cerevisiae to obtain alcoholic beverages (W-OR and W-ST, respectively). Consumer evaluation of beverages was performed using acceptability and Check-All-That-Apply (CATA) questions. Good acceptance was observed, significantly higher for W-ST than for W-OR. CATA questions gave information about organoleptic characteristics of beverages. Penalty analysis showed W-R and W-ST were positively associated with smooth/refreshing and fruity/natural, respectively. Liking was represented, accordingly with penalty analysis, by natural/refreshing. A novel alternative for utilization of whey and whey-related matrices by alcoholic beverages production with natural ingredients is presented.

Effect of thermal treatment of whey contaminated with antibiotics on the growth of Kluyveromyces marxianus.

The objective of the studies reported in this research communication was to investigate the use of whey contaminated with antibiotics such as cephalosporins, quinolones and tetracyclines as a nutrient medium for the growth of Kluyveromyces marxianus with particular attention to the effect of thermal treatment used to overcome the inhibitory effects of antibiotic concentrations close to the Maximum Residue Limits. The heat treatments at 120 °C for 40 min, 120 °C for 83 min, and 120 °C for 91 min caused total inactivation of cephalosporins, tetracyclines and quinolone residues in whey respectively.

Immobilization of Carboxypeptidase A into Modified Chitosan Matrixes by Covalent Attachment.

Carboxypeptidase A (CPA) is a metalloexopeptidase that catalyzes the hydrolysis of the peptide bonds that are adjacent to the C-terminal end of a polypeptide chain. The enzyme preferentially cleaves over C-terminal L-amino acids with aromatic or branched side chains. This is of main importance for food industry because it can be employed for manufacturing functional foods from different protein sources with reduced hydrophobic amino acid content for patients with deficiencies in the absorption or digestion of the corresponding amino acids. In that way, strategies for effective multipoint covalent immobilization of CPA metalloenzyme on chitosan beads have been developed. The study of the ability to produce several chemical modifications on chitosan molecules before, during and after its coagulation to form carrier beads lead in a protective effect of the polymer matrix. The chemical modification of chitosan through the use of an N-alkylation strategy produced the best derivatives. N-alkyl chitosan derivative beads with D-fructose presented values of 0.86 for immobilization yield, 314.6 IU g-1 bead for initial activity of biocatalyst and were 5675.64-fold more stable than the free enzyme at 55 °C. Results have shown that these derivatives would present a potential technological application in hydrolytic processes due to both their physical properties, such as low swelling capacity, reduced metal chelation ability and bulk mesoporosity, and increased operational stability when compared with soluble enzyme.

Osteotomía de Chiari en el tratamiento de las secuelas de osteoartromielitis de cadera en la infancia / Chiari osteotomy of the treatment for the sequelae of infection osteoarthritis of the hip in children

Se presentan los resultados obtenidos en el tratamiento de la secuela de osteoartromielitis de cadera en 11 pacientes: 15 caderas seguidas durante un período de hasta 40 meses. Los resultados obtenidos fueron buenos con respecto a la cobertura y función en el 60 por ciento, regulares 26,6 por ciento y malos 13,4 por ciento. Se discuten las indicaciones terapéuticas y aconsejamos las muñonplastía más osteotomía acetabular de Chiari

La cadera infantil: diagnóstico mediante ecografía de tiempo real / The infant hip: real-time ultrasound assessment

Presentamos ante esta Asociación un nuevo método diagnóstico en la patología de la cadera infantil. La necesidad de un mejor, eficaz y precoz método para el diagnóstico y tratamiento de la displasia, subluxación y luxación de la cadera es ampliamente reconocido. La ecografía de tiempo real permite la visualización de los componentes cartilaginosos de la cadera y también una evaluación directa y din mica, realizada de manera similar a un examen clínico. Se describe la utilización de 3 posiciones y las estructuras individualizadas en cada una de ellas. El diagnóstico precoz de esta patología es muy importante debido a que facilita el tratamiento y lo transforma en sumamente exitoso, sin exponerlo a las radiaciones ionizantes

Osteotomía de Chiari en el tratamiento de las secuelas de osteoartromielitis de cadera en la infancia / Chiari osteotomy of the treatment for the sequelae of infection osteoarthritis of the hip in children

Se presentan los resultados obtenidos en el tratamiento de la secuela de osteoartromielitis de cadera en 11 pacientes: 15 caderas seguidas durante un período de hasta 40 meses. Los resultados obtenidos fueron buenos con respecto a la cobertura y función en el 60 por ciento, regulares 26,6 por ciento y malos 13,4 por ciento. Se discuten las indicaciones terapéuticas y aconsejamos las muñonplastía más osteotomía acetabular de Chiari

La cadera infantil: diagnóstico mediante ecografía de tiempo real / The infant hip: real-time ultrasound assessment

Presentamos ante esta Asociación un nuevo método diagnóstico en la patología de la cadera infantil. La necesidad de un mejor, eficaz y precoz método para el diagnóstico y tratamiento de la displasia, subluxación y luxación de la cadera es ampliamente reconocido. La ecografía de tiempo real permite la visualización de los componentes cartilaginosos de la cadera y también una evaluación directa y din mica, realizada de manera similar a un examen clínico. Se describe la utilización de 3 posiciones y las estructuras individualizadas en cada una de ellas. El diagnóstico precoz de esta patología es muy importante debido a que facilita el tratamiento y lo transforma en sumamente exitoso, sin exponerlo a las radiaciones ionizantes