It was found that seven strains of bacteria can cause corrosion damage to aluminum, its alloys, and zinc. With respect to the studied metals, the most active bacteria were Proteus vulgaris 1212 and Pseudomonas aeruginosa 969. Superoxide anion radicals were demonstrated to play a role in the initiation of corrosive damage to aluminum and zinc, while bacterial exometabolites participate in the later stages of this process.
Biofilms/growth & development , Metals , Proteus vulgaris/physiology , Pseudomonas aeruginosa/physiology , Superoxides/metabolism , Corrosion
In population samples of Moscow and Tomsk, allelic polymorphism of microsatellite loci HUMCYAR04 and D19S253 was studied by polymerase chain reaction. Seven HUMCYAR04 alleles (181-205 bp) and nine alleles (208-240 bp) of the D19S253 locus were identified. In both population samples, the absence of statistically significant differences in the distribution of allele frequencies for these loci was demonstrated. The distribution of the observed genotype frequencies was shown to correspond to the Hardy-Weinberg equilibrium in both populations. Mendelian inheritance of these tandem repeats was demonstrated by an analysis of two large families. The parameters of polymorphism information content for the loci studied were determined; comparative analysis of allele frequencies with corresponding data for a number of populations was performed. These short tandem repeats were proposed for use in personal identification and paternity tests.
DNA, Satellite/genetics , Microsatellite Repeats , Polymorphism, Genetic , Alleles , Chromosome Mapping , Gene Frequency , Genotype , Humans , Moscow , Polymerase Chain Reaction , Russia , Urban Population
The method of DNA enzymatic amplification on the basis of polymerase chain reaction is described and genetic marker system criteria are defined. The method is highly sensitive. It permits gene polymorphism typing if only solitary cells are available and in case DNA is degraded. Practical use of the method is described.
Forensic Medicine/methods , HLA-DQ Antigens/genetics , Nucleic Acid Amplification Techniques , Polymorphism, Genetic , Alleles , Base Sequence , Blood Stains , DNA/genetics , DNA/isolation & purification , Genotype , HLA-DQ alpha-Chains , Hair , Humans , Male , Molecular Sequence Data , Oligonucleotide Probes , Spermatozoa
A new method for the identification of sexual appurtenance of material evidence of biologic origin on the basis of the DNA polymerase chain reaction is suggested, that permits identification of the sex even when solitary cells are available. The procedure takes but 5 hours--much less in comparison with the DNA probe employment. An example of the practical use of the method is presented.
Forensic Medicine , Polymerase Chain Reaction , Sex Determination Analysis , DNA Probes , Female , Humans , Male , Time Factors
