OBJECTIVE: To study the differences of the constituents of the volatile oil from Asarum heterotropoides var. mandshuricum with three different extracted methods. METHODS: Volatile oil from Asarum heterotropoides var. mandshuricum was extracted by steam distillation, supercritical carbon dioxide extraction and supercritical carbon dioxide extraction followed by concentration under pressure to remove volatile components, respectivily. GC-MS was utilized to analyze the components of volatile oil. RESULTS: The volatile oil from Asarum heterotropoides var. mandshuricum extracted by steam distillation and supercritical carbon dioxide extraction method contained 36 ingredients, 16 of which were the same, but different in amount. 11 kinds of non-volatile components of the volatile oil from Asarum heterotropoides var. mandshuricum were identified as the main components with supercrtical carbon dioxide extraction followed by concentration under reduced pressure, which were mainly fats and oils. CONCLUSION: The common components and their relative contents of the volatile oil from Asarum heterotropoides var. mandshuricum are obtained and compared with different extraction methods. It's the first time to extract the volatile components in the volatile oil from Asarum heterotropoides var. mandshuricum by supercritical carbon dioxide extraction, which provides the basis for Chinese herbal medicinal ingredients and quality control of the Asarum to some extent.
Asarum/chemistry , Chemical Fractionation/methods , Oils, Volatile/chemistry , Oils, Volatile/isolation & purification , Chromatography, Supercritical Fluid/methods , Gas Chromatography-Mass Spectrometry/methods , Plant Leaves/chemistry , Plant Stems/chemistry , Plants, Medicinal/chemistry , Quality Control
