Parallel detection of multiple zoonotic parasites using a real-time fluorogenic loop-mediated isothermal amplification-based quadruple-sample microfluidic chip.

Zoonotic parasites pose significant health risks globally. In the present study, we combined a microfluidic chip with loop-mediated isothermal amplification (on-chip LAMP) to detect five zoonotic parasites: Toxoplasma gondii, Cryptosporidium parvum, Cryptosporidium hominis, Clonorchis sinensis, and Taenia solium. This method enabled the simultaneous parallel analysis of five genetic markers from a maximum of four samples per chip. The on-chip LAMP assay was conducted in a highly automated format via the addition (by pipetting) of each sample in a single operation. The reaction was performed in volumes as low as 5 µL at a temperature of 65°C for 60 min, achieving limits of detection ranging from 10-2 to 10-3 pg./µL of recombinant plasmid DNA. All the time-to-positive values were less than 40 min, and almost all the coefficients of variation were less than 10%, even when using limit of detection concentrations for multiple pathogens, indicating robust reproducibility among replicates. The clinical sensitivity and specificity for detecting 135 field samples were 98.08 and 97.59%, respectively, compared with traditional biological methods, indicating good applicability in the detection of field samples. This on-chip LAMP assay allows for low reagent consumption, ease of operation, and multiple analyses of samples and genetic targets, and is applicable for on-site detection and the routine monitoring of multiple zoonotic parasites.

Postural alignment in children with bilateral spastic cerebral palsy using a bimanual interface for powered wheelchair control.

OBJECTIVE: To examine postural alignment in children with bilateral spastic cerebral palsy while driving a powered wheelchair using both a unilateral joystick and an innovative bimanual interface. DESIGN: Cross-sectional study. SUBJECTS: A total of 20 children with bilateral spastic cerebral palsy (mean age 9.0 years (standard deviation 2.1); 11 with diplegia, 9 with quadriplegia) and 14 typically developing children (mean age 7.7 years (standard deviation 2.9)). METHODS: All children drove the powered wheelchair in both the unilateral and bimanual conditions. The Seated Postural Control Measure quantified the postural alignment of subjects while driving the powered wheelchair. Statistical analysis was carried out using repeated measures analysis of variance and Spearman's rank correlation coefficient. RESULTS: As expected, typically developing children had better postural alignment in both driving conditions than children with cerebral palsy. Children with cerebral palsy demonstrated more symmetrical postural alignment while using the bimanual interface than when using the unilateral joystick. In addition, the severity of cerebral palsy correlated moderately with postural symmetry in both conditions. CONCLUSION: The results suggest that this innovative bimanual interface might be beneficial for promoting symmetrical postural alignment in some children with bilateral spastic cerebral palsy.

[Teaching experience in integrated course of human development and genetics].

Establishment of integrated course system in human development and genetics is an important part of course reformation, and the improvement of this system is achieved by integrating the content of course, stabilizing teaching force, building teaching materials and applying problem-based learning. Integrity-PBL teaching model is founded and proved to be feasible and effective by teaching practice. Therefore, it maybe play an important role in improving teaching effect and cultivating ability of students to analyse and solve problems.

[Effect of cobalt chloride on S100A4 expression in human gastric cancer cell BGC823].

S100A4 is an important metastasis-associated gene. Researches have confirmed the close correlation between overexpression of S100A4 gene and gastric cancer's infiltration, lymph node metastasis and in vitro invasiveness of gastric cancer cells. In order to investigate the mechanism of overexpression of S100A4 gene, hypoxia mimetic cobalt chloride (CoCl2) was used to treat gastric cancer cell BGC823, and then the expression of S100A4 mRNA and protein in BGC823 cells were detected by RT-PCR, immunohistochemistry, immunofluorescence, and Western blotting analysis. After treatment with CoCl2, the expression of S100A4 mRNA and protein in BGC823 cell was increased. These results suggested that hypoxia mimetic cobalt chloride could increase the expression of S100A4 gene in gastric cancer cell BGC823.