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1.
Int J Mol Sci ; 25(6)2024 Mar 08.
Artículo en Inglés | MEDLINE | ID: mdl-38542095

RESUMEN

Skin wounds and their infections by antibiotic-resistant bacteria (ARB) are very common in small animals, posing the risk of acquiring ARB by pet owners or antibiotic resistance gene (ARG) transfer to the owners' microbiota. The aim of this study was to identify the most common pathogens infecting wounds of companion animals, assess their antibiotic resistance, and determine the ARGs using culture-based, molecular, and proteomic methods. A total of 136 bacterial strains were isolated from wound swabs. Their species was identified using chromogenic media, followed by MALDI-TOF spectrometry. Antibiotic resistance was tested using disc diffusion, and twelve ARGs were detected using PCRs. The dominant species included Staphylococcus pseudintermedius (9.56%), E. coli, and E. faecalis (both n = 11, 8.09%). Enterobacterales were mostly resistant to amoxicillin/clavulanic acid (68.3% strains), all Pseudomonas were resistant to ceftazidime, piperacillin/tazobactam, imipenem, and tylosin, Acinetobacter were mostly resistant to tylosin (55.5%), all Enterococcus were resistant to imipenem, and 39.2% of Staphylococci were resistant to clindamycin. Among ARGs, strA (streptomycin resistance), sul3 (sulfonamide resistance), and blaTEM, an extended-spectrum beta-lactamase determinant, were the most frequent. The risk of ARB and ARG transfer between animals and humans causes the need to search for new antimicrobial therapies in future veterinary medicine.


Asunto(s)
Antibacterianos , Mascotas , Humanos , Animales , Antibacterianos/farmacología , Antibacterianos/uso terapéutico , Mascotas/microbiología , Escherichia coli , Tilosina , Antagonistas de Receptores de Angiotensina , Proteómica , Inhibidores de la Enzima Convertidora de Angiotensina , Bacterias/genética , Imipenem , Ecosistema , Pruebas de Sensibilidad Microbiana
2.
Procedia Comput Sci ; 192: 4741-4750, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-34659595

RESUMEN

Leadership in the age of digital transformation is facing new challenges. We observed such a case when the global coronavirus pandemic took over our lives. Leaders of Higher Education Institutions were given a unique opportunity to leverage their online presence for managing this crisis. Hence, by the means of descriptive statistics, we studied the social media accounts of 216 leaders of business schools to learn about the practices they adopted from March 2020 to March 2021. Our findings show that in this group no universal strategy of communication can be stipulated, however, some patterns are visible in different world regions.

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