RESUMEN
BACKGROUND: Using multinational collections of methicillin-susceptible Staphylococcus aureus (MSSA) isolates from infective endocarditis (IE) and soft tissue infections (STIs), we sought to (1) validate the finding that S. aureus in clonal complex (CC) 30 is associated with hematogenous complications and (2) test the hypothesis that specific genetic characteristics in S. aureus are associated with infection severity. METHODS: IE and STI isolates from 2 cohorts were frequency matched by geographic origin. Isolates underwent spa typing to infer CC and multiplex polymerase chain reaction for presence of virulence genes. RESULTS: 114 isolate pairs were genotyped. IE isolates were more likely to be CC30 (19.5% vs 6.2%; P = .005) and to contain 3 adhesins (clfB, cna, map/eap; P < .0001 for all) and 5 enterotoxins (tst, sea, sed, see, and sei; P ≤ .005 for all). CC30 isolates were more likely to contain cna, tst, sea, see, seg, and chp (P < .05 for all). CONCLUSIONS: MSSA IE isolates were significantly more likely to be CC30 and to possess a distinct repertoire of virulence genes than MSSA STI isolates from the same region. The genetic basis of this association requires further study.
Asunto(s)
Adhesinas Bacterianas/genética , ADN Bacteriano/genética , Endocarditis Bacteriana/genética , Enterotoxinas/genética , Infecciones de los Tejidos Blandos/genética , Infecciones Estafilocócicas/genética , Staphylococcus aureus/genética , Adulto , Anciano , Australia , Técnicas de Tipificación Bacteriana , Europa (Continente) , Femenino , Genotipo , Humanos , Masculino , Resistencia a la Meticilina , Persona de Mediana Edad , Medio Oriente , Tipificación de Secuencias Multilocus , Nueva Zelanda , América del Norte , Índice de Severidad de la Enfermedad , Staphylococcus aureus/patogenicidad , Factores de Virulencia/genéticaRESUMEN
The pvc gene cluster from Pseudomonas aeruginosa has been linked to the biosynthesis of both the pyoverdine chromophore and pseudoverdine. Our reinvestigation of the role this gene cluster plays in P. aeruginosa secondary metabolite biosynthesis shows that its major product is actually paerucumarin, a novel isonitrile functionalized cumarin.