Influence of different recovery intervals on time under tension, total training volume, and fatigue index in horizontal bench press exercise in young male wrestling athletes.

BACKGROUND: The recovery interval (RI) seems to be a variable closely related to the training volume since it can determine the performance after this rest time. This study investigated the influence of different recovery intervals on time under tension (TUT), total training volume (TTV), and Fatigue Index (FI) in the horizontal bench press exercise. METHODS: Eighteen male wrestling athletes underwent three visits: 1st) performed the 10-repetition maximum (10RM) test; 2nd and 3rd) performed 5 sets of up to 10 repetitions with 1 minute (RI1) and 3 minutes (RI3) of passive RI with randomized entry. TUT, number of repetitions, TTV and FI data were collected or calculated. RESULTS: TUT was lower in sets 5 (P<0.001) for RI1 when compared to RI3, with no significant difference for the other 4 sets. The number of repetitions for RI1 was lower when compared to RI3 in sets 3 (P=0.018), 4 (P=0.023), and 5 (P<0.001), with no significant difference in sets 1 and 2. The FI was significantly higher for RI1 (P<0.001); however, TTV was significantly higher for RI3 (P=0.007). CONCLUSIONS: Different RI influenced the TUT and the number of repetitions along 5 sets in the horizontal bench press exercise. Moreover, these two variables showed different behavior when compared under the same condition (RI1 or RI3), especially after the third set. Using longer RI demonstrated a greater ability to maintain TTV and less negative effect of fatigue in young male wrestling athletes.

Characterization of microbial community composition, antimicrobial resistance and biofilm on intensive care surfaces.

BACKGROUND: Organisms causing healthcare associated infections can be sourced from the inanimate environment around patients. Residing in a biofilm increases the chances of these organisms persisting in the environment. We aimed to characterise bacterial environmental contamination, genetically and physiologically, and relate this to general intensive care unit (ICU) cleanliness. METHODS: Cleanliness was determined by adenosine triphosphate (ATP) measurements of 95 high-touch objects. Bacteriological samples were obtained from the same sites (n=95) and from aseptically removed sections (destructive samples, n=20). Bacterial enrichment culture was conducted using tryptone soya broth prior to plating on horse blood agar, MacConkey agar, and screening chromogenic agar for identification of multidrug resistance organism (MDRO). Bacterial load and microbial diversity were determined using quantitative PCR (qPCR) and next generation DNA sequencing respectively. Confocal laser scanning microscopy and scanning electron microscopy were used to visually confirm the biofilm presence. RESULTS: Many intensive care surfaces (61%) were highly contaminated by biological soil as determined by ATP bioluminescence testing. The degree of biological soiling was not associated with bacterial contamination as detected by qPCR. Bacterial load ranged from 78.21 to 3.71×108 (median=900) bacteria/100cm2. Surface swabs from 71/95 sites (75%) were culture-positive; of these 16 (22.5%) contained MDRO. The most abundant genera were Staphylococcus, Propionibacterium, Pseudomonas, Bacillus, Enterococcus, Streptococcus and Acinetobacter. Biofilm was visually confirmed by microscopy on 70% (14/20) of items. CONCLUSION: Bacterial biofilms and MDROs were found on ICU surfaces despite regular cleaning in Saudi Arabia, suggesting that biofilm development is not controlled by current cleaning practices.