SPME-GC-MS untargeted metabolomics approach to identify potential volatile compounds as markers for fraud detection in roasted and ground coffee.

Roasted ground coffee has been intentionally adulterated for economic revenue. This work aims to use an untargeted strategy to process SPME-GC-MS data coupled with chemometrics to identify volatile compounds (VOCs) as possible markers to discriminate Arabica coffee and its main adulterants (corn, barley, soybean, rice, coffee husks, and Robusta coffee). Principal Component Analysis (PCA) showed the difference between roasted ground coffee and adulterants, while the Hierarchical Clustering of Principal Components (HCPC) and heat map showed a trend of adulterants separation. The partial Least-Squares Discriminant Analysis (PLS-DA) approach confirmed the PCA results. Finally, 24 VOCs were putatively identified, and 11 VOCs are candidates for potential markers to detect coffee fraud, found exclusively in one type of adulterant: coffee husks, soybean, and rice. The results for possible markers may be suitable for evaluating the authenticity of ground-roasted coffee, thus acting as a coffee fraud control and prevention tool.

[Factorial structure of the questionnaire for the inclusion of in natura vegetable oil in the diet of children]. / Estrutura fatorial do questionário de adoção do óleo vegetal in natura de acréscimo na dieta infantil.

The scope of this study was to investigate the factorial structure of the questionnaire for the inclusion of vegetable oil in natura in the diet of children. A cross-sectional study was conducted with 220 mothers using the Likert Scale questionnaire. Thirteen Child Nutrition experts/judges semantically evaluated the items and the Generalizability Coefficient (GC) assessed the errors according to a one-off, two-facet random model (item/judge). The Exploratory Factor Analysis (EFA) indicated dimensionality of the tools applied: Doornik-Hansen Test; Main Factor Analysis; Kaiser Guttman criterion; Promax rotation; Kaiser-Meyer-Olkin test (KMO); convergent and discriminant validities, Cronbach's Alpha; Composite Reliability. The GC = 0.8407 was considered satisfactory. The Doornik-Hansen test (chi2 (28) = 198.053, prob>chi2 = 0.0000) confirmed the multivariate normality of data. The Kaiser Guttman criterion explained 76.32% of the latent construct. The correlation between factors 1 and 2 (-0.4042) suggests the two-dimensional nature of the instrument. The KMO (0.8440) indicated the adequacy of the data to perform EFA. Cronbach's alpha showed values around 0.70, considered acceptable. The two-dimensionality of the questionnaire was verified to assess the inclusion of in natura vegetable oils in the diet of children.

O objetivo foi verificar a estrutura fatorial do questionário de adoção do óleo vegetal in natura de acréscimo na dieta infantil. Estudo transversal, com 220 mães de crianças, e questionário em escala Likert. Treze juízes, especialistas em nutrição infantil, avaliaram semanticamente os itens, enquanto que o Coeficiente de Generalização (CG) mensurou erros de avaliação, segundo modelo randômico de duas facetas (item/juiz), uma ocasião. A Análise Fatorial Exploratória (AFE) indicou dimensionalidade do instrumento aplicado: teste Doornik-Hansen, Análise de Fator Principal, critério Kaiser Guttman, rotação Promax, teste Kaiser-MeyerOlkin (KMO), validades convergente e discriminante, coeficiente alfa de Cronbach, Confiabilidade Composta. O CG = 0,8407 foi considerado satisfatório. O teste Doornik-Hansen (Qui2 (28) = 198,053, prob > Qui2 = 0,0000) inferiu sobre a normalidade multivariada dos dados. O critério de Kaiser Guttman indicou 76,32% da latência do construto. A correlação entre os fatores 1 e 2 (-0,4042) sugere a bidimensionalidade do instrumento. KMO (0,8740) indicou a adequação dos dados para AFE. O alfa de Cronbach apresentou valores em torno de 0,70, considerados aceitáveis. Verificou-se bidimensionalidade do questionário para avaliar a adoção da prática do acréscimo de óleos vegetais in natura na dieta infantil.

Estrutura fatorial do questionário de adoção do óleo vegetal in natura de acréscimo na dieta infantil / Factorial structure of the questionnaire for the inclusion of in natura vegetable oil in the diet of children

Resumo O objetivo foi verificar a estrutura fatorial do questionário de adoção do óleo vegetal in natura de acréscimo na dieta infantil. Estudo transversal, com 220 mães de crianças, e questionário em escala Likert. Treze juízes, especialistas em nutrição infantil, avaliaram semanticamente os itens, enquanto que o Coeficiente de Generalização (CG) mensurou erros de avaliação, segundo modelo randômico de duas facetas (item/juiz), uma ocasião. A Análise Fatorial Exploratória (AFE) indicou dimensionalidade do instrumento aplicado: teste Doornik-Hansen, Análise de Fator Principal, critério Kaiser Guttman, rotação Promax, teste Kaiser-MeyerOlkin (KMO), validades convergente e discriminante, coeficiente alfa de Cronbach, Confiabilidade Composta. O CG = 0,8407 foi considerado satisfatório. O teste Doornik-Hansen (Qui2 (28) = 198,053, prob > Qui2 = 0,0000) inferiu sobre a normalidade multivariada dos dados. O critério de Kaiser Guttman indicou 76,32% da latência do construto. A correlação entre os fatores 1 e 2 (-0,4042) sugere a bidimensionalidade do instrumento. KMO (0,8740) indicou a adequação dos dados para AFE. O alfa de Cronbach apresentou valores em torno de 0,70, considerados aceitáveis. Verificou-se bidimensionalidade do questionário para avaliar a adoção da prática do acréscimo de óleos vegetais in natura na dieta infantil.

Abstract The scope of this study was to investigate the factorial structure of the questionnaire for the inclusion of vegetable oil in natura in the diet of children. A cross-sectional study was conducted with 220 mothers using the Likert Scale questionnaire. Thirteen Child Nutrition experts/judges semantically evaluated the items and the Generalizability Coefficient (GC) assessed the errors according to a one-off, two-facet random model (item/judge). The Exploratory Factor Analysis (EFA) indicated dimensionality of the tools applied: Doornik-Hansen Test; Main Factor Analysis; Kaiser Guttman criterion; Promax rotation; Kaiser-Meyer-Olkin test (KMO); convergent and discriminant validities, Cronbach's Alpha; Composite Reliability. The GC = 0.8407 was considered satisfactory. The Doornik-Hansen test (chi2 (28) = 198.053, prob>chi2 = 0.0000) confirmed the multivariate normality of data. The Kaiser Guttman criterion explained 76.32% of the latent construct. The correlation between factors 1 and 2 (-0.4042) suggests the two-dimensional nature of the instrument. The KMO (0.8440) indicated the adequacy of the data to perform EFA. Cronbach's alpha showed values around 0.70, considered acceptable. The two-dimensionality of the questionnaire was verified to assess the inclusion of in natura vegetable oils in the diet of children.