Evaluation of Dual-Frequency Switching HIFU for Optimizing Superficial Ablation.

OBJECTIVE: Dual-frequency high-intensity focused ultrasound (HIFU) thermal ablation is an exceptionally promising technique for treating tumors due to its precision and effectiveness. However, there are still a few studies on improving the accuracy and efficiency of HIFU in superficial ablation applications. This study proposes a method utilizing dual frequency switching ultrasound (DFSU) to enhance the efficiency and precision of superficial treatments. METHODS: A dual-frequency HIFU transducer operating at 4.5 MHz and 13.7 MHz was designed, and a dual-frequency impedance matching network was designed to optimize electro-acoustic conversion efficiency. Phantom and ex vivo tests were conducted to measure and compare thermal lesion areas and temperature rises caused by single-frequency ultrasound (SFU) and DFSU. RESULTS: In both phantom and ex vivo tests, the utilization of DFSU resulted in larger lesion areas compared to SFU. Moreover, DFSU provided improved control and versatility, enabling precise and efficient ablation. CONCLUSION: DFSU exhibits the ability to generate larger ablation areas in superficial tissue compared to SFU, and DFSU allows flexible control over the ablation area and temperature rise rate. The acoustic power deposition of HIFU can be optimized to achieve precise ablation.

Dual-Frequency Impedance Matching Network Design Using Genetic Algorithm for Power Ultrasound Transducer.

Dual-frequency ultrasounds have demonstrated significant potential in augmenting thermal ablation efficiency for tumor treatment. Ensuring proper impedance matching between the dual-frequency transducer and the power amplifier system is imperative for equipment safety. This paper introduces a novel dual-frequency impedance matching network utilizing L-shaped topology and employing a genetic algorithm to compute component values. Implementation involved an adjustable capacitor and inductor network to achieve dual-frequency matching. Subsequently, the acoustic parameters of the dual-frequency HIFU transducer were evaluated before and after matching, and the effects of ultrasound thermal ablation with and without matching were compared. The proposed dual-frequency impedance matching system effectively reduced the standing wave ratio at the two resonance points while enhancing transmission efficiency. Thermal ablation experiments with matching circuits showed improved temperature rise efficiencies at both frequencies, resulting in an expanded ablation zone. The dual-frequency impedance matching method significantly enhances the transmission efficiency of the dual-frequency ultrasound system at two operational frequencies, thereby ensuring equipment safety. It holds promising prospects for application in dual-frequency ultrasound treatment.

Multi-Frequency Ultrasound Imaging Fusion Method Based on Wavelet Transform for Guided Screw Insertion.

Intraosseous ultrasound imaging can serve as a guiding tool for the placement of pedicle screws during spinal fusion surgery; thus far, there has been limited scholarly exploration of methods for intraosseous multifrequency ultrasound imaging, which can achieve simultaneous high resolution and deep penetration. The proposed method introduced a dynamic fusion strategy grounded in wavelet transformation for multifrequency image decomposition. This strategy accomplished the effective amalgamation of high-frequency ultrasound images and low-frequency ultrasound images, enabling the obtaining of fused images with enhanced details and better overall image quality. A novel near-field effect elimination method was also proposed to improve the quality of ultrasound imaging in the near-field region. Experimental evaluations were conducted on isolated bovine circle bone and sheep spine with pedicle screw tracks. The fusion images are capable of effectively detecting areas within the pedicle screw track that have either ruptured or are in close proximity to rupture, even measuring the size of breaches. Evaluation criteria, including information entropy (IE), spatial frequency (SF), average gradient (AG), mutual information (MI), structural similarity index (SSIM), and edge information-based image fusion quality metric (QAB/F), were employed to assess the fusion performance; moreover, the influence of mother wavelet function selection and decomposition levels on computational complexity and fusion image quality was thoroughly discussed. The proposed method exhibited promising potential for intraosseous imaging navigation, which can aid in accurate diagnosis, treatment planning, and monitoring in fields such as orthopedics, surgery, and interventional procedures.

Ultrathin endoscope equipped with ultrasonic miniprobe for upper GI US in a porcine model.

BACKGROUND AND AIMS: Ultrathin EGD (UT-EGD) is an ideal tool for unsedated upper GI examination and pediatric gastroenterology but is rarely competent for EUS miniprobe (EUS-MP). We developed a UT-EGD US method (UT-EUS) and verified its clinical application value through animal experiments. METHODS: Five Bama miniature pigs were selected. Using an acoustic medium, we performed US on the duodenum, stomach, and esophagus, respectively, with conventional 20-MHz EUS miniprobe (EUS-MP-20), 20-MHz UT-EUS (UT-EUS-20), and 30-MHz UT-EUS (UT-EUS-30). The times to acquire 5 consecutive stable US images, number of identifiable wall layers, and quality and penetration depth of the images were recorded. RESULTS: No significant differences were found in the time required to obtain images between EUS-MP-20 and UT-EUS-20 at each site (P > .05). UT-EUS-30 showed more wall levels than UT-EUS-20 (P < .05). No significant differences were noted between EUS-MP-20 and UT-EUS-20 in imaging quality and penetration depth (P > .05). CONCLUSIONS: The UT-EUS is easy to use with a satisfactory image quality and has potential clinical application value.

Evaluation of the dual-frequency transducer for controlling thermal ablation morphology using frequency shift keying signal.

PURPOSE: The catheter-based ultrasound (CBUS) can reach the target tissue directly and achieve rapid treatment. The frequency shift keying (FSK) signal is proposed to regulate and evaluate tumor ablation by a miniaturized dual-frequency transducer. METHODS: A dual-frequency transducer prototype (3 × 7 × 0.4 mm) was designed and fabricated for the CBUS applicator (OD: 3.8 mm) based on the fundamental frequency of 5.21 MHz and the third harmonic frequency of 16.88 MHz. Then, the acoustic fields and temperature field distributions using the FSK signals (with 0, 25, 50, 75, and 100% third harmonic frequency duty ratios) were simulated by finite element analysis. Finally, tissue ablation and temperature monitoring were performed in phantom and ex vivo tissue, respectively. RESULTS: At the same input electrical power (20 W), the output acoustic power of the fundamental frequency of the transducer was 10.03 W (electroacoustic efficiencies: 50.1%), and that of the third harmonic frequency was 6.19 W (30.6%). As the third harmonic frequency duty ratios increased, the shape of thermal lesions varied from strip to droplet in simulated and phantom experimental results. The same trend was observed in ex vivo tests. CONCLUSION: Dual-frequency transducers excited by the FSK signal can control the morphology of lesions. SIGNIFICANCE: The acoustic power deposition of CBUS was optimized to achieve precise ablation.

High-Frequency Endoscopic Ultrasound Imaging With Phase-Corrected-and-Sum and Coherence Factor Weighting.

High-frequency endoscopic ultrasound (HFEUS) imaging is an important tool commonly used in clinical practice for imaging hollow organs. The virtual source synthetic aperture (VSSA) method is effective in improving the imaging quality of HFEUS. However, interference from the motor control unit severely affects the accuracy of the conventional delay and sum (DAS) method, thus compromising the effectiveness of VSSA. In this article, a new computational method based on phase correction was proposed to overcome these shortcomings, which is named phase-corrected-and-sum (PCAS). Meanwhile, the parameters of coherence factor weighting (CFW) can be obtained from the correlation coefficient of the superimposed signals to further increase the imaging quality. Three kinds of imaging experiments were designed to evaluate the proposed method. Compared with the conventional method, the results show that the PCAS-CFW method improves the lateral resolution by about 10% and the contrast-to-noise ratio (CNR) by about 44%. Therefore, this proposed method is capable of significantly improving HFEUS image quality, and this method can be easily integrated into current HFEUS imaging systems, showing great potential for clinical applications.

A high frequency endoscopic ultrasound imaging method combining chirp coded excitation and compressed sensing.

Insufficient imaging penetration and large data acquisition are two of the major challenges of high-frequency ultrasound imaging. Based on the good autocorrelation properties of chirp signal and the feasibility of using compressed sensing theory to reconstruct high-quality ultrasound images with low sampling requirements, this paper proposed a chirp coded excitation combined with compressed sensing (CCE-CS) technique for high-frequency endoscopic ultrasound (HFEUS) imaging. The feasibility of the method was verified by a brief theoretical analysis, and the relevant parameters were selected and analyzed according to the actual engineering situation. Simulated phantoms and in-vitro tissue experiments were used to evaluate the performance of the CCE-CS. Simulation results demonstrate that CCE-CS is capable of reducing the impact of reconstruction errors and improving imaging quality through comparison with conventional methods. The reduction of reconstruction data had less impact on penetration depth, resolution and general contrast general contrast-to-noise ratio (gCNR), and the reconstructed image was closer to the original image with a maximum improvement of 37% in peak signal-to-noise ratio (PSNR). Moreover, comparisons were conducted on the digestive tract of swine, and the results show that CCE-CS is also feasible in the in-vitro environment. These results demonstrated that CCE-CS method has good potential for application to improve the imaging quality of HFEUS while reducing the sampling rate.

A High-Frequency Mechanical Scanning Ultrasound Imaging System.

High-frequency ultrasound has developed rapidly in clinical fields such as cardiovascular, ophthalmology, and skin with its high imaging resolution. However, the development of multi-elements high-frequency ultrasonic transducers and multi-channel high-frequency ultrasound imaging systems is extremely challenging. Here, a high-frequency ultrasound imaging system based on mechanical scanning was proposed in this paper. It adopts the method of reciprocating feed mechanism, which can achieve reciprocating scanning in the 14 mm range at 168 mm/s with a small 60 MHz transducer. A single-channel high-frequency ultrasonic imaging system consisting of the transmitting module, analog front end, acquisition module, and FPGA control module was developed. To overcome the non-uniformity of mechanical scanning, the ultrasound images are compensated according to the motion trajectory. The wire target and ex vivo tissue experiments have shown that the system can obtain an imaging resolution of 51 µm, imaging depth of 8 mm, and imaging speed of 12 fps. This high-frequency mechanical scanning ultrasound imaging system has the characteristics of simple structure, high-frequency, real-time, and good imaging performance, which can meet the clinical needs of high-resolution ultrasound images.

Efficacy Estimation of Microbubble-Assisted Local Sonothrombolysis Using a Catheter with a Series of Miniature Transducers.

Intravascular ultrasound has good prospects for clinical applications in sonothrombolysis. The catheter-based side-looking intravascular ultrasound thrombolysis (e.g., Ekosonic catheters) used in clinical studies has a high frequency (2 MHz). The lower-frequency ultrasound requires a larger-diameter transducer. In our study, we designed and manufactured a small ultrasound-based prototype catheter that can emit a lower frequency ultrasound (1.1 MHz). In order to evaluate the safety and efficacy of local low-frequency ultrasound-enhanced thrombolysis, a microbubble (MB) was introduced to augment thrombolysis effect of locally delivered low-intensity ultrasound. The results demonstrated that combination of ultrasound and MB realized higher clot lysis than urokinase-only treatment (17.0% ± 1.2% vs. 14.9% ± 2.7%) under optimal ultrasound settings of 1.1 MHz, 0.414 MPa, 4.89 W/cm2, 5% duty cycle and MB concentration of 60 µg/mL. When urokinase was added, the fibrinolysis accelerated by MB and ultrasound resulted in a further increased thrombolysis rate that was more than two times than that of urokinase alone (36.7% ± 5.5% vs. 14.9% ± 2.7%). However, a great quantity of ultrasound energy was required to achieve substantial clot lysis without MB, leading to the situation that temperature accumulated inside the clot became harmful. We suggest that MB-assisted local sonothrombolysis be considered as adjuvant therapy of thrombolytic agents.

Erratum: Jiang et al. Localization Accuracy of Ultrasound-Actuated Needle with Color Doppler Imaging. Diagnostics 2020, 10, 1020.

The authors wish to correct the following erratum in this paper [...].

Development of high frequency piezocomposite with hexagonal pillars via cold ablation process.

This paper reports on the fabrication of 1-3 piezocomposite with hexagonal pillars for high frequency ultrasonic transducer based on the cold ablation technique. The piezocomposite with hexagonal pillars was designed, simulated, and fabricated using an ultraviolet picosecond laser. It performs better than the piezocomposite with other pillar shapes like square. The edge length and height of the hexagonal PZT pillar were 10 µm and 36 µm, the width of the kerf was about 5 µm. The 1-3 piezocomposite with a resonance frequency of 51.2 MHz and a coupling coefficient of 0.69 was fabricated. The transducer with fabricated 1-3 piezocomposite was prototyped and characterized. Compared to the conventional dice-and-fill technique, the cola ablation process allows for the manufacturing of 1-3 piezocomposites with higher variability of pillar design and distribution as well as smaller structural size. It suggests that the cold ablation process proves to be suitable for the fabrication of high frequency composite and transducers.

Excitation emission matrix fluorescence spectroscopy and parallel factor framework-clustering analysis for oil pollutants identification.

Serious ecological damage can be caused due to increased oil pollution. Identifying the source of oil can inform effective mitigation strategies and policies. A novel method for oil pollutants identification has been presented based on excitation emission matrix (EEM) fluorescence spectroscopy and parallel factor framework-clustering analysis (PFFCA). First, the EEM spectroscopy of the oil samples was measured by a FS920 steady-state fluorescence spectrometer. Second, EEM was analyzed and characterized by PFFCA. A total 90 EEM were decomposed into six components using excitation wavelengths from 260 to 400 nm and emission wavelengths from 280 to 450 nm. Finally, oil samples were classified and matched by using concentration vectors. The results were compared with those obtained by using linear discriminant analysis (LDA) employing parallel factor analysis (PARAFAC) scores, and partial least squares discriminant analysis (PLS-DA). The best classification result was obtained by using LDA employing concentration vectors with 96.7% accuracy. The results indicate that PFFCA-LDA offers a robust approach for the oil samples, which is of great significance in discrimination of oil pollutants.

Micromachined High Frequency 1-3 Piezocomposite Transducer Using Picosecond Laser.

In this article, a PZT/Epoxy 1-3 piezoelectric composite based on picosecond laser etching technology is developed for the fabrication of high-frequency ultrasonic transducer. The design, fabrication, theoretical analysis, and performance of the piezocomposite and transducer are presented and discussed. According to the test results, the area of the PZT pillar is [Formula: see text], the average width of the kerf is [Formula: see text], and the thickness of the piezocomposite is [Formula: see text]. The fabricated 1-3 piezocomposite has a resonant frequency of 46.5 MHz, a parallel resonant frequency of 65 MHz, and an electromechanical coupling coefficient of 0.73. According to the wires phantom imaging, its imaging resolution can reach [Formula: see text]. This study shows that the proposed picosecond laser micromachining technique can be applied in the fabrication of high frequency 1-3 piezocomposite and transducer.

Micro Non-Uniform Linear Array (MNULA) for Ultrasound Plane Wave Imaging.

Ultrasound plane wave imaging technology has been applied to more clinical situations than ever before because of its rapid imaging speed and stable imaging quality. Most transducers used in plane wave imaging are linear arrays, but their structures limit the application of plane wave imaging technology in some special clinical situations, especially in the endoscopic environment. In the endoscopic environment, the size of the linear array transducer is strictly miniaturized, and the imaging range is also limited to the near field. Meanwhile, the near field of a micro linear array has serious mutual interferences between elements, which is against the imaging quality of near field. Therefore, we propose a new structure of a micro ultrasound linear array for plane wave imaging. In this paper, a theoretical comparison is given through sound field and imaging simulations. On the basis of primary work and laboratory technology, micro uniform and non-uniform linear arrays were made and experimented with the phantom setting. We selected appropriate evaluation parameters to verify the imaging results. Finally, we concluded that the micro non-uniform linear array eliminated the artifacts better than the micro uniform linear array without the additional use of signal processing methods, especially for target points in the near-field. We believe this study provides a possible solution for plane wave imaging in cramped environments like endoscopy.

An Improved Chirp Coded Excitation Based on Compression Pulse Weighting Method in Endoscopic Ultrasound Imaging.

Chirp coded excitation is an effective method to improve the signal-to-noise ratio (SNR) and penetration depth of high-frequency endoscopic ultrasound (EUS) imaging. In coded excitation, pulse compression is applied to compress the elongated coded signals into a short pulse, which determines the final imaging performance, including spatial resolution and SNR. However, with the current pulse compression methods, it is hard to get high performance in the peak sidelobe level (PSL), image contrast, and axial resolution at the same time. To solve this problem, in this article, a new method named compressed pulse weighting method (CPWM) was proposed based on the combination of two kinds of pulse compression signals. A brief theoretical derivation proved the feasibility of method. The proposed method was evaluated by the simulation and phantom experiments. Compared with traditional method, the results showed that the proposed adaptive weighting method can provide increases of 32.42% in the penetration depth, 9.48 dB in the SNR, 5.60 dB in the contrast ratio (CR), 5.46 in the contrast-to-noise ratio (CNR), and 0.13 mm in the axial imaging resolution for 12-MHz EUS. Therefore, this method can effectively improve the ultrasound penetration depth and imaging quality, which made it have good potential for high-frequency ultrasound imaging.

Localization Accuracy of Ultrasound-Actuated Needle with Color Doppler Imaging.

An ultrasonic needle-actuating device for tissue biopsy and regional anaesthesia offers enhanced needle visibility with color Doppler imaging. However, its specific performance is not yet fully determined. This work investigated the influence on needle visibility of the insertion angle and drive voltage, as well as determined the accuracy and agreement of needle tip localization by comparing color Doppler measurements with paired photographic and B-mode ultrasound measurements. Needle tip accuracy measurements in a gelatin phantom gave a regression trend, where the slope of trend is 0.8808; coefficient of determination (R2) is 0.8877; bias is -0.50 mm; and the 95% limits of agreement are from -1.31 to 0.31 mm when comparing color Doppler with photographic measurements. When comparing the color Doppler with B-mode ultrasound measurements, the slope of the regression trend is 1.0179; R2 is 0.9651; bias is -0.16 mm; and the 95% limits of agreement are from -1.935 to 1.605 mm. The results demonstrate the accuracy of this technique and its potential for application to biopsy and ultrasound guided regional anaesthesia.

Multimodal Detection for Cryptogenic Epileptic Seizures Based on Combined Micro Sensors.

The cryptogenic epilepsy of the neocortex is a disease in which the seizure is accompanied by intense cerebral nerve electrical activities but the lesions are not observed. It is difficult to locate disease foci. Electrocorticography (ECoG) is one of the gold standards in seizure focus localization. This method detects electrical signals, and its limitations are inadequate resolution which is only 10 mm and lack of depth information. In order to solve these problems, our new method with implantable micro ultrasound transducer (MUT) and photoplethysmogram (PPG) device detects blood changes to achieve higher resolution and provide depth information. The basis of this method is the neurovascular coupling mechanism, which shows that intense neural activity leads to sufficient cerebral blood volume (CBV). The neurovascular coupling mechanism established the relationship between epileptic electrical signals and CBV. The existence of mechanism enables us to apply our new methods on the basis of ECoG. Phantom experiments and in vivo experiments were designed to verify the proposed method. The first phantom experiments designed a phantom with two channels at different depths, and the MUT was used to detect the depth where the blood concentration changed. The results showed that the MUT detected the blood concentration change at the depth of 12 mm, which is the position of the second channel. In the second phantom experiments where a PPG device and MUT were used to monitor the change of blood concentration in a thick tube, the results showed that the trend of superficial blood concentration change provided by the PPG device is the same as that provided by the MUT within the depth of 2.5 mm. Finally, in the verification of in vivo experiments, the blood concentration changes on the surface recorded by the PPG device and the changes at a certain depth recorded by the MUT all matched the seizure status shown by ECoG. These results confirmed the effectiveness of the combined micro sensors.

[Design of Intravascular Ultrasound-enhanced Thrombolysis Excitation System Based on FPGA].

An intravascular ultrasound-enhanced thrombolysis excitation system with adjustable frequency, amplitude and duty cycle was designed based on FPGA (ZYNQ-7Z020). Firstly, the FPGA generated waveform amplitude binary data based on direct digital frequency synthesis (DDS) technology, and then the data was converted into burst signal through an external daughter card, which included D/A conversion circuit, active low-pass filter, power amplifier circuit and impedance matching circuit. The test results demonstrated that the output waveform reached the target with advantages of simple implementation and flexible control, the peak negative pressure generated from ultrasound transducer was doubled by means of an electrical impedance matching network. In vitro thrombus models were applied to verify the excitation system, it turned out that ultrasound cavitation effect generated could accelerate the penetration of urokinase and increase the thrombolysis rate by about 20%.

Hydrodynamic flow cytometer performance enhancement by two-dimensional acoustic focusing.

Conventional flow cytometers employ hydrodynamic focusing method to insure detection accuracy by forcing cells go through detected position. However, an increased flow velocity will significantly reduce detection precision due to a fact that cells will deviate center position and are easily silted in choke point. In an effort to overcome this limitation, a two-dimension ultrasonic particle focusing method are presented in this work to enhance the performance of flow cytometer. Two piezoelectric transducers are used to attach to a 250 µm × 250 µm rectangular fused silica flow channel to realize the modification. Finite element model simulation is performed for parametrical analysis and simplifying experiment design. 3 µm polystyrene fluorescent particles are adopted to test focusing effect. One dimension acoustic focusing is achieved at 2.95 MHz with single focusing node as well as 2, 3, 4 nodes focusing near 6, 9, 12 MHz respectively. The 2D focusing particle stream width in two dimensions is less than 10 µm. Results verified that this method is applicable for Jurkat cells. Sample flow maintains its stability without clogging up even at high sample concentration. Focusing still works at flow velocity over 100 µl/min. All these results certify this acoustic particles focusing method can enhance the performance of hydrodynamic flow cytometer by minor modification.

Sensitivity Enhanced Photoacoustic Imaging Using a High-Frequency PZT Transducer with an Integrated Front-End Amplifier.

Photoacoustic (PA) imaging is a hybrid imaging technique that can provide both structural and functional information of biological tissues. Due to limited permissible laser energy deposited on tissues, highly sensitive PA imaging is required. Here, we developed a 20 MHz lead zirconium titanate (PZT) transducer (1.5 mm × 3 mm) with front-end amplifier circuits for local signal processing to achieve sensitivity enhanced PA imaging. The electrical and acoustic performance was characterized. Experiments on phantoms and chicken breast tissue were conducted to validate the imaging performance. The fabricated prototype shows a bandwidth of 63% and achieves a noise equivalent pressure (NEP) of 0.24 mPa/√Hz and a receiving sensitivity of 62.1 µV/Pa at 20 MHz without degradation of the bandwidth. PA imaging of wire phantoms demonstrates that the prototype is capable of improving the detection sensitivity by 10 dB compared with the traditional transducer without integrated amplifier. In addition, in vitro experiments on chicken breast tissue show that structures could be imaged with enhanced contrast using the prototype and the imaging depth range was improved by 1 mm. These results demonstrate that the transducer with an integrated front-end amplifier enables highly sensitive PA imaging with improved penetration depth. The proposed method holds the potential for visualization of deep tissue structures and enhanced detection of weak physiological changes.