Hemolytic-Pred: A machine learning-based predictor for hemolytic proteins using position and composition-based features.

Objective: The objective of this study is to propose a novel in-silico method called Hemolytic-Pred for identifying hemolytic proteins based on their sequences, using statistical moment-based features, along with position-relative and frequency-relative information. Methods: Primary sequences were transformed into feature vectors using statistical and position-relative moment-based features. Varying machine learning algorithms were employed for classification. Computational models were rigorously evaluated using four different validation. The Hemolytic-Pred webserver is available for further analysis at http://ec2-54-160-229-10.compute-1.amazonaws.com/. Results: XGBoost outperformed the other six classifiers with an accuracy value of 0.99, 0.98, 0.97, and 0.98 for self-consistency test, 10-fold cross-validation, Jackknife test, and independent set test, respectively. The proposed method with the XGBoost classifier is a workable and robust solution for predicting hemolytic proteins efficiently and accurately. Conclusions: The proposed method of Hemolytic-Pred with XGBoost classifier is a reliable tool for the timely identification of hemolytic cells and diagnosis of various related severe disorders. The application of Hemolytic-Pred can yield profound benefits in the medical field.

iTAGPred: A Two-Level Prediction Model for Identification of Angiogenesis and Tumor Angiogenesis Biomarkers.

A crucial biological process called angiogenesis plays a vital role in migration, growth, and wound healing of endothelial cells and other processes that are controlled by chemical signals. Angiogenesis is the process that controls the growth of blood vessels within tissues while angiogenesis proteins play a significant role in the proper working of this process. The balancing of these signals is necessary for the proper working of angiogenesis. Unbalancing of these signals increases blood vessel formation, which causes abnormal growth or several diseases including cancer. The proposed work focuses on developing a two-layered prediction model using different classifiers like random forest (RF), neural network, and support vector machine. The first level performs in silico identification of angiogenesis proteins based on the primary structure. In the case the protein is an angiogenesis protein, then the second level predicts whether the protein is linked with tumor angiogenesis or not. The performance of the model is evaluated through various validation techniques. The model was evaluated using k-fold cross-validation, independent, self-consistency, and jackknife testing. The overall accuracy using an RF classifier for angiogenesis at the first level was 97.8% and for tumor angiogenesis at the second level was 99.5%, ANN showed 94.1% accuracy for angiogenesis and 79.9% for tumor angiogenesis, and the accuracy of SVM for angiogenesis was 78.8% and for tumor angiogenesis was 65.19%.