Impact of hydrodynamics on community structure and metabolic production of marine biofouling formed in a highly energetic estuary.

Biofouling is a specific lifestyle including both marine prokaryotic and eukaryotic communities. Hydrodynamics are poorly studied parameters affecting biofouling formation. This study aimed to investigate how water dynamics in the Etel Estuary (Northwest Atlantic coasts of France) influences the colonization of artificial substrates. Hydrodynamic conditions, mainly identified as shear stress, were characterized by measuring current velocity, turbulence intensity and energy using Acoustic Doppler Current Profiler (ADCP). One-month biofouling was analyzed by coupling metabarcoding (16S rRNA, 18S rRNA and COI genes), untargeted metabolomics (liquid chromatography coupled with high-resolution mass spectrometry, LC-HRMS) and characterization of the main biochemical components of the microbial exopolymeric matrix. A higher richness was observed for biofouling communities (prokaryotes and eukaryotes) exposed to the strongest currents. Ectopleura (Cnidaria) and its putative symbionts Endozoicomonas (Gammaproteobacteria) were dominant in the less dynamic conditions. Eukaryotes assemblages were specifically shaped by shear stress, leading to drastic changes in metabolite profiles. Under high hydrodynamic conditions, the exopolymeric matrix increased and was composed of 6 times more polysaccharides than proteins, these latter playing a crucial role in the adhesion and cohesion properties of biofilms. This original multidisciplinary approach demonstrated the importance of shear stress on both the structure of marine biofouling and the metabolic response of these complex communities.

Assessing the turbulent kinetic energy budget in an energetic tidal flow from measurements of coupled ADCPs.

Two coupled four-beam acoustic Doppler current profilers were used to provide simultaneous and independent measurements of the turbulent kinetic energy (TKE) dissipation rate ε and the TKE production rate [Formula: see text] over a 36 h long period at a highly energetic tidal energy site in the Alderney Race. The eight-beam arrangement enabled the evaluation of the six components of the Reynolds stress tensor which allows for an improved estimation of the TKE production rate. Depth-time series of ε, [Formula: see text] and the Reynolds stresses are provided. The comparison between ε and [Formula: see text] was performed by calculating individual ratios of ε corresponding to [Formula: see text]. The depth-averaged ratio [Formula: see text] averaged over whole flood and ebb tide were found to be 2.2 and 2.8 respectively, indicating that TKE dissipation exceeds TKE production. It is shown that the term of diffusive transport of TKE is significant. As a result, non-local transport is important to the TKE budget and the common assumption of a local balance, i.e. a balance between production and dissipation, is not valid at the measurement site. This article is part of the theme issue 'New insights on tidal dynamics and tidal energy harvesting in the Alderney Race'.

Characterization of the vertical evolution of the three-dimensional turbulence for fatigue design of tidal turbines.

A system of two coupled four-beam acoustic Doppler current profilers was used to collect turbulence measurements over a 36-h period at a highly energetic tidal energy site in Alderney Race. This system enables the evaluation of the six components of the Reynolds stress tensor throughout a large proportion of the water column. The present study provides mean vertical profiles of the velocity, the turbulence intensity and the integral lengthscale along the streamwise, spanwise and vertical direction of the tidal current. Based on our results and considering a tidal-stream energy convertor (TEC) aligned with the current main direction, the main elements of turbulence prone to affect the structure (material fatigue) and to alter power generation would likely be: (i) the streamwise turbulence intensity (Ix), (ii) the shear stress, [Formula: see text], (iii) the normal stress, [Formula: see text] and (iv) the vertical integral lengthscale (Lz). The streamwise turbulence intensity, (Ix), was found to be higher than that estimated at other tidal energy sites across the world for similar height above bottom. Along the vertical direction, the length (Lz) of the large-scale turbulence eddies was found to be equivalent to the rotor diameter of the TEC Sabella D10. It is considered that the turbulence metrics presented in this paper will be valuable for TECs designers, helping them optimize their designs as well as improve loading prediction through the lifetime of the machines. This article is part of the theme issue 'New insights on tidal dynamics and tidal energy harvesting in the Alderney Race'.