Nutrient removal and biomass production of marine microalgae cultured in recirculating aquaculture systems (RAS) water with low phosphate concentration.

This study was conducted to investigate the feasibility of microalgal biomass production and nutrient removal from recirculating aquaculture systems (RAS) water (RASW) with low phosphate concentration. For this purpose, Nannochloropsis oculata, Pavlova gyrans, Tetraselmis suecica, Phaeodactylum tricornutum, and their consortium were cultivated in RASW and RASW supplemented with vitamins (+V). Among them, N. oculata showed the maximum biomass production of 0.4 g/L in RASW. Vitamins supplementation significantly increased the growth of T. suecica from 0.16 g/L in RASW to 0.33 g/L in RASW + V. Additionally, T. suecica showed the highest nitrate (NO3-N) removal efficiency of 80.88 ± 2.08 % in RASW and 83.82 ± 2.08 % in RASW + V. Accordingly, T. suecica was selected for scaling up study of microalgal cultivation in RASW and RASW supplemented with nitrate (RASW + N) in 4-L airlift photobioreactors. Nitrate supplementation enhanced the growth of T. suecica up to 2.2-fold (day 15). The fatty acid nutritional indices in T. suecica cultivated in RASW and RASW + N showed optimal polyunsaturated fatty acids (PUFAs)/saturated fatty acid (SFAs), omega-6 fatty acid (n-6)/omega-3 fatty acid (n-3), indices of atherogenicity (IA), and thrombogenicity (IT)). Overall, the findings of this study revealed that despite low phosphate concentration, marine microalgae can grow in RASW and relatively reduce the concentration of nitrate. Furthermore, the microalgal biomass cultivated in RASW consisting of pigments and optimal fatty acid nutritional profile can be used as fish feed, thus contributing to a circular bioeconomy.

Removal and recovery of nutrients from septic tank wastewater using microalgae: Key factors and practical implications.

Treatment of septic tank wastewater (STWW) with high concentrations of ammonium (NH4+) and total phosphorus (TP), is challenging in decentralized areas. Utilizing microalgae for STWW treatment can simultaneously recover nutrients in the form of high-value microalgal biomass. However, despite the potential benefits, microalgal treatment of STWW is rarely reported. Therefore, this work utilized bench-scale photobioreactors (PBR) to investigate different factors that could affect microalgal cultivation in STWW and treatment efficiency. Accordingly, it was observed that suspended solids present in STWW did not significantly affect the microalgae growth and nutrient removal efficiencies in bubble column PBR. On the other hand, the effect of endemic microorganism could not be verified in this study due to observed fungal contamination and change in nutrient profile of STWW after autoclave. Nevertheless, the highest microalgal growth and nutrient removal efficiencies of NH4+-N = 79.14% and TP = 41.11% were observed within 14 days of photoautotrophic cultivation in raw STWW. Further, 25 days of upscaled photoautotrophic cultivation in 4-L bubble column PBR was performed to study biomass yield, nutrient removal kinetics, and nutrient removal efficiency. Consequently, 0.75 g‧L-1 dry biomass was produced with improved removal efficiency of NH4+-N (96.16%), and TP (69.57%). Elemental analysis of biomass revealed that 62.99 ± 1.46 mg‧L-1 TN and 11.41 ± 1.42 mg‧L-1 TP were recovered. Further, 1.02 geq carbon dioxide (CO2) was bio-fixed with every liter of STWW treated. The findings of this study revealed that microalgae can be successfully utilized for the removal and recovery of nutrients from STWW.

Enhanced production of bioactive compounds from marine microalgae Tetraselmis tetrathele under salinity and light stresses: A two-stage cultivation strategy.

This study leveraged the salinity and light intensity stresses during the stationary phase for enhancing the pigment contents and antioxidant capacity of Tetraselmis tetrathele. The highest pigments content was obtained in cultures under salinity stress (40 g L-1) illuminated using fluorescent light. Furthermore, the best inhibitory concentration (IC50) for scavenging the 2, 2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radicals was found as 79.53 µg mL-1 in ethanol extract and cultures under red LED light stress (300 µmol m-2 s-1). The highest antioxidant capacity in a ferric-reducing antioxidant power (FRAP) assay (1,778.6 µM Fe+2) was found in ethanol extract and cultures under salinity stress illuminated using fluorescent light. Maximum scavenging of the 2.2-diphenyl-1-picrylhydrazyl (DPPH) radical was found in ethyl acetate extracts under light and salinity stresses. These results indicated that abiotic stresses could enhance the pigment and antioxidant components of T. tetrathele, which are value-added compounds in the pharmaceutical, cosmetic, and food industries.

Applications of soft computing techniques for prediction of pollutant removal by environmentally friendly adsorbents (case study: the nitrate adsorption on modified hydrochar).

Artificial intelligence has emerged as a powerful tool for solving real-world problems in various fields. This study investigates the simulation and prediction of nitrate adsorption from an aqueous solution using modified hydrochar prepared from sugarcane bagasse using an artificial neural network (ANN), support vector machine (SVR), and gene expression programming (GEP). Different parameters, such as the solution pH, adsorbent dosage, contact time, and initial nitrate concentration, were introduced to the models as input variables, and adsorption capacity was the predicted variable. The comparison of artificial intelligence models demonstrated that an ANN with a lower root mean square error (0.001) and higher R2 (0.99) value can predict nitrate adsorption onto modified hydrochar of sugarcane bagasse better than other models. In addition, the contact time and initial nitrate concentration revealed a higher correlation between input variables with the adsorption capacity.

The effects of light regime on carbon cycling, nutrient removal, biomass yield, and polyhydroxybutyrate (PHB) production by a constructed photosynthetic consortium.

Microalgae can add value to biological wastewater treatment processes by capturing carbon and nutrients and producing valuable biomass. Harvesting small cells from liquid media is a challenge easily addressed with biofilm cultivation. Three experimental photobioreactors were constructed from inexpensive materials (e.g. plexiglass, silicone) for hybrid liquid/biofilm cultivation of a microalgal-bacterial consortia in aquaculture effluent. Three light regimes (full-spectrum, blue-white, and red) were implemented to test light spectra as a process control. High-intensity full-spectrum light caused photoinhibition and low biomass yield, but produced the most polyhydroxybutyrate (PHB) (0.14 mg g-1); a renewable bioplastic polymer. Medium-intensity blue-white light was less effective for carbon capture, but removed up to 82 % of phosphorus. Low-intensity red light was the only net carbon-negative regime, but increased phosphorus (+4.98 mg/L) in the culture medium. Light spectra and intensity have potential as easily-implemented process controls for targeted wastewater treatment, biomass production, and PHB synthesis using photosynthetic consortia.

Synthesis of biochar from iron-free and iron-containing microalgal biomass for the removal of pharmaceuticals from water.

The contamination of natural water bodies with pharmaceutical compounds has raised significant concerns about ecological and public health safety. In this study, biochars were synthesized from iron-free microalgal biomass (harvested by centrifugation) and iron-containing microalgal biomass (harvested by coagulation) and tested for the adsorption of ciprofloxacin (CIP) and diclofenac (DIC) from water in batch and fixed-bed column continuous studies. The physicochemical properties of synthesized biochars were analyzed using Brunauer, Emmett and Teller (BET) surface area analyzer, elemental analyzer, Fourier Transformed Infrared spectroscopy (FTIR), X-ray Diffractometer (XRD), and Scanning electron microscope with energy dispersive spectroscopy (SEM-EDS). The maximum monolayer adsorption capacities of iron-containing biochar (FBC750W) and iron-free biochar (MBC750W) based on the Langmuir model were obtained as 75.97 mg/g and 39.08 mg/g for CIP, and 40.99 mg/g and 6.77 mg/g for DIC, respectively. Comparatively, maximum monolayer adsorption capacities of commercial activated carbon (C-AC) were found to be 50.97 mg/g and 46.39 mg/g for CIP and DIC, respectively. In fixed-bed column continuous adsorption studies, the effects of flow rate (1 and 2 mL/min) and the adsorbent amount (50 and 100 mg) on adsorption performance were evaluated. Column kinetic models, such as Bohart-Adams model and Fractal-like Bohart-Adams model were examined. The adsorption mechanisms were proposed as pore filling, π-π interaction, and electrostatic interaction. Overall, the results of this study revealed that microalgal biomass, harvested with FeCl3, can be used for the direct synthesis of iron-containing biochar for the removal of pharmaceuticals from water.

Synthesis and Characterization of a Magnetic Carbon Nanofiber Derived from Bacterial Cellulose for the Removal of Diclofenac from Water.

Engineering and synthesis of novel materials are vital for removing emerging pollutants, such as pharmaceuticals from contaminated water. In this study, a magnetic carbon nanofiber (MCF) fabricated from bacterial cellulose was tested for the adsorption of diclofenac from water. The physical and chemical properties of the synthesized adsorbent were examined by field emission scanning electron microscopy (FESEM), field emission transmission electron microscopy (FETEM), X-ray diffraction (XRD), Brunauer-Emmett-Teller (BET) analysis, energy-dispersive X-ray spectroscopy (EDS), a vibrating sample magnetometer (VSM), Raman spectroscopy, and Fourier transform infrared (FTIR) spectroscopy. The characterization results showed that the MCF is a carbon nanofiber with a three-dimensional interconnect network, forming a porous material (mesopores and macropores) with a specific surface area of 222.3 m2/g. The removal of diclofenac (10 mg/L) by the MCF (0.75 g/L) was efficient (93.2%) and fast (in 20 min). According to the Langmuir isotherm model fitting, the maximum adsorption capacity of the MCF was 43.56 mg/g. Moreover, continuous adsorption of diclofenac onto MCF was investigated in a fixed-bed column, and the maximum adsorption capacity was found to be 67 mg/g. The finding of this research revealed that the MCF could be a promising adsorbent used to remove diclofenac from water, while it can be easily recovered by magnetic separation.

Recent advances in the application of microalgae and its derivatives for preservation, quality improvement, and shelf-life extension of seafood.

Seafood is a highly perishable food product due to microbiological, chemical, and enzymatic reactions, which are the principal causes of their rapid quality deterioration. Therefore, ever-increasing consumers' demand for high-quality seafood along with a negative perception of synthetic preservatives creates opportunities for natural preservatives such as microalgae extracts. They are potential alternatives to reduce microbial growth, increase oxidative stability, and protect the sensorial properties of seafood. Research has shown that the inclusion of microalgae extracts into the aquatic animal's diet could enhance their meat quality and increase production. This review focuses on the direct application of various microalgae extracts as seafood preservative, and their functional properties in seafood, such as antioxidant and antimicrobial activities. Besides, the potential nutritional application of microalgae extracts as an alternative in aqua-feed and their impact on seafood quality (indirect application) are also presented. The safety aspects and regulatory issues of products from microalgae are highlighted.

The effect of solvents polarity and extraction conditions on the microalgal lipids yield, fatty acids profile, and biodiesel properties.

This study reports the effects of polar (acetone/methanol) and non-polar (chloroform/hexane) solvents on lipid yield, fatty acids methyl esters (FAMEs) composition, and biodiesel properties of microalgae. The lipids yield extracted by hexane and chloroform (100.01 and 94.33 mg/g) were higher than by methanol and acetone (40.12 and 86.91 mg/g). The polarity of solvents also affected FAMEs composition of microalgal lipids. Total saturated fatty acids and unsaturated fatty acids of extracted lipids were 61.53% and 38.47% by chloroform and 38.85% and 61.15% by methanol. Moreover, polar and non-polar solvents affected the biodiesel properties such as cetane number and oxidative stability. In addition, higher ratio of chloroform to methanol and higher temperature increased the lipid yield and saturation degree of lipids, through ultrasound-assisted lipid extraction method. Overall, the results revealed that the lipids yield, FAMEs composition, and biodiesel quality of microalgal biomass can be significantly affected by solvents polarity and extraction conditions.

Insights into upstream processing of microalgae: A review.

The aim of this review is to provide insights into the upstream processing of microalgae, and to highlight the advantages of each step. This review discusses the most important steps of the upstream processing in microalgae research such as cultivation modes, photobioreactors design, preparation of culture medium, control of environmental factors, supply of microalgae seeds and monitoring of microalgal growth. An extensive list of bioreactors and their working volumes used, elemental composition of some well-known formulated cultivation media, different types of wastewater used for microalgal cultivation and environmental variables studied in microalgae research has been compiled in this review from the vast literature. This review also highlights existing challenges and knowledge gaps in upstream processing of microalgae and future research needs are suggested.

Synthesis and characterization of magnetic biochar adsorbents for the removal of Cr(VI) and Acid orange 7 dye from aqueous solution.

In this study, different types of magnetic biochar nanocomposites were synthesized using the co-precipitation method. Two biochar materials, namely, sewage sludge biochar and woodchips biochar, were prepared at two different temperatures, viz., 450 and 700 °C. These biochars were further modified with magnetic nanoparticles (Fe3O4). The modified biochar nanocomposites were characterized using field emission-scanning electron microscopy (FE-SEM), X-ray diffraction (XRD), transmission electron microscopy (TEM), Brunauer-Emmett-Teller (BET), SQUID analysis, X-ray photoelectron spectroscopy (XPS), and Fourier-transform infrared spectroscopy (FTIR). The potential of prepared adsorbents was examined for the removal of hexavalent chromium (Cr(VI)) and Acid orange 7 (AO7) dye from water as a function of various parameters, namely, contact time, pH of solution, amount of adsorbents, and initial concentrations of adsorbates. Various kinetic and isotherm models were tested to discuss and interpret the adsorption mechanisms. The maximum adsorption capacities of modified biochars were found as 80.96 and 110.27 mg g-1 for Cr(VI) and AO7, respectively. Magnetic biochars showed high pollutant removal efficiency after 5 cycles of adsorption/desorption. The results of this study revealed that the prepared adsorbents can be successfully used for multiple cycles to remove Cr(VI) and AO7 from water. Graphical Abstract.

Hexavalent chromium removal from water by microalgal-based materials: Adsorption, desorption and recovery studies.

The current study presents a comprehensive comparison towards the potential of different microalgal-based materials for the removal of hexavalent chromium (Cr(VI)) from water. Among the tested materials, microalgal biochar showed the highest removal efficiency (100%) of Cr(VI). The highest monolayer estimated adsorption capacities were 23.98, 25.19 and 24.27 mg/g at 5, 22 and 35 °C, respectively. Experimental data showed good compliance with pseudo-second-order kinetic model. The results of continuous column studies showed that the column removal efficiency increased from 52.33 to 57.58% by increasing the adsorbent dose from 0.125 to 0.200 g. Desorption efficiency of Cr(VI) by 0.1 M NaOH was increased from 51.16 to 59.41% by sonication bath as compared to roller shaker. More than 97% of desorbed Cr(VI) was recovered in less than 10 min by BaCl2. This study shows that non-living microalga materials are more effective than living cells in the removal and recovery of Cr(VI) from water.

Sequential cultivation of microalgae in raw and recycled dairy wastewater: Microalgal growth, wastewater treatment and biochemical composition.

In this study, two cycles of mixotrophic and one cycle of heterotrophic cultivation of Scenedesmus quadricauda (freshwater) and Tetraselmis suecica (marine water) microalgae in dairy wastewater (DWW) were investigated. Dry weights of S. quadricauda and T. suecica were found to be 0.43 and 0.58 g/L after the first cycle and 0.36, and 0.65 g/L after the second cycle of mixotrophic cultivation, respectively. Chlorophyll a content of both microalgae in the first cycle was significantly higher than the second cycle. S. quadricauda removed 92.15% of total nitrogen, 100% of phosphate, 100% of sulfate and 76.77% of total organic carbon, after two cycles of cultivation. The dominant fatty acids during the first and second cycle of S. quadricauda and T. suecica cultivation were C18:1 and C18:3n-3, respectively. The results suggest that by reusing DWW in two consecutive cycles of microalgal cultivation, higher pollutants removal efficiency and microalgal biomass production can be achieved.

Photocatalytic degradation of toxic aquatic pollutants by novel magnetic 3D-TiO2@HPGA nanocomposite.

In this study, a series of photocatalysts were prepared, namely bare 3D-TiO2 (b-3D-T), magnetic 3D-TiO2: (m3D-T) and magnetic 3D-TiO2@Hierarchical Porous Graphene Aerogels (HPGA) nanocomposite: (m3D-T-HPGA NC) by solvothermal process. The prepared photocatalysts were analyzed by using X-ray diffraction (XRD), Field emission scanning electron microscope (FE-SEM), transmission electron microscope (TEM), Vibrating sample magnetometer (VSM), Brunauer-Emmett-Teller (BET) and Diffuse Reflectance Measurement - Ultraviolet (DRS-UV) to know their physical and chemical properties. The photocatalytic degradations of two toxic aquatic pollutants viz., Cr(VI) and bisphenol A (BPA) were tested by using the prepared photocatalysts. Parameters such as initial pollutant concentration, solution pH, photocatalyst dosage, wavelength and light intensity were investigated to optimize the process. The photocatalytic properties of prepared catalyst were analyzed based on the degradation of Cr(VI) and BPA under UV irradiation. The modified photocatalysts showed better performance as compared to b-3D-T photocatalyst. This better performance is ascribed to efficient charge transfer between b-3D-T nanoparticles to the porous graphene sheets. The maximum photocatalytic degradation of Cr(VI) was found to be 100% with m3D-T-HPGA NC within 140 min, whereas a removal efficacy of 100% and 57% was noticed in case of m3D-T and b-3D-T within 200 and 240 min, respectively. In the case of BPA, the maximum degradation efficiency was found to be 90% with m3D-T-HPGA NC within 240 min.

Versatile applications of freshwater and marine water microalgae in dairy wastewater treatment, lipid extraction and tetracycline biosorption.

In this study, freshwater (Scenedesmus quadricauda, Sq) and marine water (Tetraselmis suecica, Ts) microalgae were used for the treatment of dairy wastewater (DWW). Sq and Ts showed the highest biomass productivity as 0.47 and 0.61 g/L, respectively. Removal efficiencies of total nitrogen (TN), phosphate (PO43-), and total organic carbon (TOC) were observed as 86.21, 89.83 and 64.47% by Sq and 44.92, 42.18 and 40.16% by Ts, respectively. After wastewater treatment, lipids were extracted from microalgal biomasses. Fatty acid methyl esters (FAMEs) analysis revealed that saturated fatty acids (SFAs) are dominant in Sq and polyunsaturated fatty acids (PUFAs) in Ts. After lipid extraction, removal of tetracycline (TC) from water by microalgal biomasses was also investigated. Maximum adsorption capacities of Sq and Ts were found to be 295.34 and 56.25 mg/g, respectively. Results of this study revealed the versatile applications of microalgae for wastewater treatment, lipid production and TC removal from water.

Investigation on the feasibility of Chlorella vulgaris cultivation in a mixture of pulp and aquaculture effluents: Treatment of wastewater and lipid extraction.

In this study, feasibility of Chlorella vulgaris cultivation in pulp wastewater (PWW) diluted with lake water (LW) and aquaculture wastewater (AWW) was investigated. The best ratios of PWW and AWW (PAWW) viz., 80% PWW:20% AWW and 60% PWW:40% AWW were selected as microalgal culture medium. Algal growth was investigated with and without addition of macro and micronutrients to the cultivation medium. The highest dry algal weight was observed as 1.31 g/L in 60% PWW:40% AWW without adding micronutrients. Nutrients and organic compounds removal efficiencies by microalga were studied in PAWW. Protein, carbohydrate and lipid percentage of harvested microalga from wastewater and Bold's Basal Medium (BBM) solution were analyzed. Fatty acids analysis revealed that C16 and C18 are the major fatty acids in C. vulgaris cultivated in BBM and PAWW. The results of this study revealed that C. vulgaris is a potential candidate for PAWW treatment and lipid and carbohydrate accumulation.

Hematological parameters of Iranian cichlid Iranocichla hormuzensis: Coad, 1982 (Perciformes) in Mehran river.

This study describes the hematological parameters in Iranocichla hormuzensis, an Iranian freshwater cichlid important as ornamental and food fish. Forty fish were captured with seine net at Mehran river Hormozgan province, Iran. Blood was used to determine the total counts of red blood cells (RBC) and white blood cells (WBC), hematocrit, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC) and morphometric data of erythrocytes. The Iranian fish showed lower RBC and WBC values than the other cichlids (Oreochromis niloticus, O. aureus, O. mossambicus, O. hybrid, Cichlasoma dimerus and Cichla monoculus). Hematocrit did not vary among the species, but MCV, MCH and MCHC in I. hormuzensis were higher than those for O. niloticus, O. aureus, O. hybrid, C. dimerus and C. monoculus. These differences may be related to different life habit of fish. This study suggests that I. hormuzensis is well acclimated to the environment being the first report for its hematology. It is also suggested high efficiency in oxygen transportation, and an efficient inflow of oxygen by the gills, indicating the welfare of fish on this environment.