1.
Sci Rep
; 13(1): 20529, 2023 11 22.
Article
En
| MEDLINE
| ID: mdl-37993476
Accurate protein quantitation is essential for many cellular mechanistic studies. Existing technology relies on extrinsic sample evaluation that requires significant volumes of sample as well as addition of assay-specific reagents and importantly, is a terminal analysis. This study exploits the unique chemical features of a fluorescent molecular rotor that fluctuates between twisted-to-untwisted states, with a subsequent intensity increase in fluorescence depending on environmental conditions (e.g., viscosity). Here we report the development of a rapid, sensitive in situ protein quantitation method using ARCAM-1, a representative fluorescent molecular rotor that can be employed in both non-terminal and terminal assays.
Fluorescent Dyes , Fluorescent Dyes/chemistry , Viscosity , Fluorescence
2.
Obstet Gynecol
; 104(6): 1424; author reply 1424-5, 2004 Dec.
Article
En
| MEDLINE
| ID: mdl-15572511