Cool as a moose: How can browsing counteract climate warming effects across boreal forest ecosystems?

Herbivory has potential to modify vegetation responses to climatic changes. However, climate and herbivory also affect each other, and rarely work in isolation from other ecological factors, such as plant-plant competition. Thus, it is challenging to predict the extent to which herbivory can counteract, amplify, or interact with climate impacts on ecosystems. Here, we investigate how moose modify climatic responses of boreal trees by using experimental exclosures on two continents and modeling complex causal pathways including several climatic factors, multiple tree species, competition, tree height, time, food availability, and herbivore presence, density, and browsing intensity. We show that moose can counteract, that is, "cool down" positive temperature responses of trees, but that this effect varies between species depending on moose foraging preferences. Growth of preferred deciduous trees was strongly affected by moose, whereas growth of less preferred conifers was mostly driven by climate and tree height. In addition, moose changed temperature responses of rowan in Norway and balsam fir in Canada, by making fir more responsive to temperature but decreasing the strength of the temperature response of rowan. Snow protected trees from browsing, and therefore moose "cooling power" might increase should a warming climate result in decreased snow cover. Furthermore, we found evidence of indirect effects of moose via plant-plant competition: By constraining growth of competing trees, moose can contribute positively to the growth of other trees. Our study shows that in boreal forests, herbivory cooling power is highly context dependent, and in order to understand its potential to prevent changes induced by warming climate, species differences, snow, competition, and climate effects on browsing need to be considered.

Ex situ germination as a method for seed viability assessment in a peatland orchid, Platanthera blephariglottis.

UNLABELLED: • PREMISE OF THE STUDY: Assessing seed quality in orchids has been hindered by stringent germination requirements. Seed quality has traditionally been assessed in orchids using in vitro or in situ germination protocols or viability staining. However, these methods are not always well suited for rapid assessment of viability in the context of ecological studies.• METHODS: The potential of an ex situ protocol for seed viability assessment of orchids in ecological studies was investigated by sowing seeds of Platanthera blephariglottis on Sphagnum moss collected in the orchid's natural habitat. Ex situ germination results were compared with those obtained by viability staining using triphenyltetrazolium chloride (TTC), and the effect of seed testa color on staining and germination results was investigated.• KEY RESULTS: The ex situ protocol yielded high germination rates, with 66% of the seeds germinating after 9 wk. Depending on the seed testa color class, ex situ germination rates were about 1.4 to 2.5 times higher than viability rates determined using TTC, indicating that the TTC technique underestimated viability compared with the method using ex situ germination. The TTC estimates of viability rates were higher for seeds with dark-colored testae than for pale ones, whereas seed testa color had no effect on germination.• CONCLUSIONS: Our study showed promising results for the use of ex situ germination as an alternative to previously developed protocols for seed viability assessment of orchids in ecological studies. Staining using TTC might not be well suited for this purpose, since it introduced a bias with respect to seed testa color.

The importance of ecological constraints on the control of multi-species treeline dynamics in eastern Nunavik, Quebec.

PREMISE OF THE STUDY: Treelines are temperature-sensitive ecotones that should be able to expand in response to global warming; however, they are also controlled by ecological constraints. These constraints can create bottlenecks for tree regeneration, hindering treeline advances. Near Kangiqsualujjuaq (Nunavik, subarctic Québec), previous studies suggested successful recruitment of Larix laricina above the altitudinal treeline, while Picea mariana establishment remains scarce. We studied regeneration of both species to identify factors responsible for such contrasting responses. • METHODS: We measured seeds and wings to evaluate species dispersal potential. We compared seed viability and tolerance to shrub leachates with germination trials. To evaluate seedbed preferences, we compared seedling occurrence on the different seedbeds with seedbed relative abundance in the field. • KEY RESULTS: Seed germination was similar between L. laricina and P. mariana, whereas dispersal potential was higher for the latter. Germination of P. mariana seeds was more strongly inhibited by shrub leachates than were L. laricina seeds. In the field, we found only a few Picea seedlings, but numerous seedlings of Larix had established disproportionally on several seedbeds. While Betula glandulosa, mosses, and Vaccinium uliginosim impeded Larix establishment, numerous seedlings were found on lichens, mineral soil, and liverworts. The low occurrence of suitable seedbeds for Picea, mainly mineral soil, could explain the seedling scarcity of this species. • CONCLUSIONS: This study highlighted that allelopathy and unsuitable seedbeds could contribute to regeneration failure of P. mariana in eastern Nunavik and emphasizes the need to consider ecological preferences of species before predicting treeline expansion under a warmer climate.