RESUMEN
In recent times, ultra-thin films of hafnium oxide (HfO2) have shown ferroelectricity (FE) attributed to the orthorhombic (o) phase of HfO2 with space group Pca21. This polar o-phase could be stabilized in the doped thin film of the oxide. In the present work, both polar and non-polar o-phases of HfO2 could be stabilized in Gd-doped bulk polycrystalline HfO2. Rietveld analysis of XRD data shows that the relative population of o-phases in the presence of the monoclinic (m) phase of HfO2 increases with increasing Gd-content. The local environment around the host atom has been investigated by time differential perturbed angular correlation (TDPAC) spectroscopy, synchrotron based X-ray near edge structure (XANES) and extended X-ray absorption fine structure (EXAFS) measurements. Field emission scanning electron microscopy (FESEM) and transmission electron microscopy (TEM) measurements showed a reduction in grain size with increasing Gd-dopant indicating a solute drag effect. It could be established that the segregation of the Gd-dopant in the grain boundary is a thermodynamically favorable process and the solute drag effect plays an important role in nucleation of the o-phase in bulk HfO2. Stabilization of Gd in both Pbca and Pca21 phases of HfO2 was supported by defect formation energy calculations using density functional theory (DFT). The present study has important implications in future applications of HfO2 in ferroelectric devices and in understanding the role of dopants in stabilizing the o-phase of HfO2 in the bulk.
RESUMEN
Temperature dependent phase transformation behavior in cobalt from hexagonal close-packed (hcp) to face centered cubic (fcc) has been found to be contradictory to that reported earlier. It is found that hcp phase stabilizes at both low and high temperature ([Formula: see text]873 K) while fcc phase is stabilized at [Formula: see text]500 K. At 298 K, hcp Co has been found to be predominant ([Formula: see text]70%) where hcp magnetic phase is [Formula: see text]60%. At 973 K, hcp phase is again predominant ([Formula: see text]73%), but it is mainly the non-magnetic phase ([Formula: see text]67%). Contrary to present results, it was found earlier that fcc phase was stabilized at high temperature and hcp to fcc transformation occured at [Formula: see text]700 K. Present results from perturbed angular correlation measurements, therefore, requires a new theoretical interpretation for Co phase transformation. From present measurements, hyperfine magnetic fields in Co at room temperature for the hcp and fcc phases have been found to be 18.7(6) and 12.8(3) T, much lower than earlier reported results. The hyperfine magnetic fields at [Formula: see text]Ta impurity atom have been calculated by density functional theory (DFT) employing the full potential (linearized) augmented plane wave method (FP-LAPW). Present calculated results for both hcp and fcc phases corroborate our experimental results.
RESUMEN
The recent observation of ferroelectricity in ultra thin films of hafnium oxide (HfO2) has been attributed to the orthorhombic (o) phase of HfO2 with space group Pca21. Although this oxide is polymorphic in nature, this polar o-phase is known to be stabilized in the doped thin film oxide. The objective of the present experiment is to stabilize the o-phases in La doped bulk polycrystalline HfO2 and investigate their evolution with the doping concentration through Time Differential Perturbed Angular Correlation (TDPAC), X-ray Absorption Near Edge Structure (XANES) and Extended X-ray Absorption Fine Structure (EXAFS) measurements. The present work reports the presence of both the polar Pca21 phase and the antipolar Pbca phase at different La-concentrations. Two o-phases of HfO2 with space groups Pca21 and Pbca, difficult to distinguish by other complimentary methods, could be unambiguously identified by utilizing the atomic scale sensitivity of the electric field gradient (EFG) embedded in TDPAC spectroscopy. The determination of the oxidation state and the local environment of La-atoms by XANES and EXAFS measurements illuminates the microscopic role of the dopant in stabilizing the o-phase. The "solute drag model" proposes a critical crystallite size for the nucleation of the o-phase in bulk HfO2 and explains the role of the La-dopant in stabilizing the o-phase. Thus the present study shows the possibility of stabilizing the polar o-phase and hence attaining ferroelectricity in bulk HfO2 to augment the scope of future application for this ferroelectric device.
RESUMEN
Understanding the impacts of plastic pollution is a global research priority. Previous research has shown that plasticizers such as phthalate esters detected in seabird tissues can be useful non-lethal biochemical markers of plastic ingestion as compared with more standard necropsy techniques. We examined the concentrations of six phthalate esters in the preen oil of Northern Fulmars (Fulmarus glacialis) in relation to their retained plastics. Contrary to a previous study, we found that the phthalates examined were not analytically detectable in fulmar preen oil. Given that the birds we examined had up to 100 pieces of plastics in their stomachs, and all uropygial glands were completely emptied during the necropsies, it does not appear that measuring phthalates in preen oil of Northern Fulmars is a useful, non-lethal technique to determine if individuals ingest plastics, at least not currently given the available commercial analytical detection limits.
Asunto(s)
Aves , Exposición Dietética/análisis , Monitoreo del Ambiente , Contenido Digestivo , Plásticos , Animales , Ésteres , Ácidos FtálicosRESUMEN
From temperature dependent perturbed angular correlation (PAC) measurements (77-873 K) in equiatomic TiNi intermetallic alloy, martensitic phase transformations have been observed. Three frequency components corresponding to three different phases of TiNi have been found in the temperature range 298-873 K. The results of quadrupole frequency and asymmetry parameters at room temperature are found to be: ωQ = 14(1) Mrad/s, η = 0 (33%), ωQ = 40.0(5) Mrad/s, η = 0.66(3) (52%) and ωQ = 56.7(3)Mrad/s, η = 0.39(2) (15%). The frequency component with η = 0 and which enhances to ~52% at 373 K can be attributed to the cubic austenite phase. The predominant component (~52%) found at room temperature has been attributed to monoclinic martensitic phase of TiNi and the third component with values of ωQ and η similar to those for the martensitic phase is attributed to the intermediate orthorhombic phase. At 77 K, no intermediate and austenite phases have been found but only the martensite phase is observed at this temperature. From XRD measurements at room temperature also, three phases of TiNi have been observed.
RESUMEN
PURPOSE: To compare changes in inferior vena cava (IVC) filter positional parameters from insertion to removal and examine how they affect retrievability amongst various filter types. MATERIALS AND METHODS: A total of 447 patients (260 men, 187 women) with a mean age of 55 years (range: 13-91 years) who underwent IVC filter retrieval between 2007-2014 were retrospectively included. Post-insertion and pre-retrieval angiographic studies were assessed for filter tilt, migration, strut wall penetration and retrieval outcomes. ANCOVA and multiple logistic regression models were used to analyze factors affecting retrieval success. Pairwise comparisons between filter types were performed. RESULTS: Of 488 IVC filter retrieval attempts, 94.1% were ultimately successful. The ALN filter had the highest mean absolute value of tilt (5.6 degrees), the Optease filter demonstrated the largest mean migration (-8.0mm) and the Bard G2 filter showed highest mean penetration (5.2mm). Dwell time of 0-90 days (OR, 11.1; P=0.01) or 90-180 days (OR, 2.6; P=0.02), net tilt of 10-15 degrees (OR 8.9; P=0.05), caudal migration of -10 to 0mm (OR, 3.46; P=0.03) and penetration less than 3mm (OR, 2.6; P=0.01) were positive predictors of successful retrievability. Higher odds of successful retrieval were obtained for the Bard G2X, Bard G2 and Cook Celect when compared to the ALN and Cordis Optease filters. CONCLUSION: Shorter dwell time, lower mean tilt, caudal migration and less caval wall penetration are positive predictors of successful IVC filter retrieval.
Asunto(s)
Remoción de Dispositivos , Filtros de Vena Cava , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Angiografía , Femenino , Migración de Cuerpo Extraño/complicaciones , Humanos , Masculino , Persona de Mediana Edad , Diseño de Prótesis , Estudios Retrospectivos , Resultado del Tratamiento , Vena Cava Inferior/diagnóstico por imagen , Adulto JovenRESUMEN
This study evaluates height loss during the day in light of variables assessable through participant self-report. Participants were 19 female and 15 male staff and students who met with us twice with a mean interval between measurement sessions of 6.96h (SD=0.86). Hypotheses were evaluated using two-stage least squares regression analysis (SYSTAT 10). Males and females lost similar amounts of height between sessions (male,x¯=6.9mm;female,x¯=7.4mm). Among factors considered to impact overall height loss, sleep duration (Adj. R2=0.181, p=0.022) and height (Adj. R2=0.121, p=0.048) were useful in univariate analyses, though they were not significant in any models that included variables other than sex. Judging from log-transformed BMI variation, heavier males and females lost more height (F(2, 31)=4.59, Adj. R2=0.179, p=0.018). Among factors anticipated to reduce height loss by acting prior to morning measurements, only time spent walking was significantly associated (ß=2.6±0.8mm, t=3.16, p=0.004) when included as a predictor along with sex (p=0.17) and log-BMI (p=0.003). This model explained about 38% of height loss variance. None of the factors considered as potentially acting between measurement sessions showed statistically significant influences when included in the model just described, though predictor coefficients were in the anticipated direction. Results suggest that self-reported activities may be an important supplement to anthropometric studies, both for planning and later evaluation, particularly in large studies.
Asunto(s)
Estatura/fisiología , Ritmo Circadiano/fisiología , Adolescente , Adulto , Anciano , Antropometría , Índice de Masa Corporal , Femenino , Humanos , Análisis de los Mínimos Cuadrados , Masculino , Persona de Mediana Edad , Modelos Biológicos , Autoinforme , Caracteres Sexuales , Sueño/fisiología , Caminata/fisiología , Adulto JovenRESUMEN
Many animals use coloration to communicate with other individuals. Although the signalling role of avian plumage colour is relatively well studied, there has been much less research on coloration in avian bare parts. However, bare parts could be highly informative signals as they can show rapid changes in coloration. We measured bill colour (a ubiquitous bare part) in over 1600 passerine species and tested whether interspecific variation in carotenoid-based coloration is consistent with signalling to potential mates or signalling to potential rivals in a competitive context. Our results suggest that carotenoid bill coloration primarily evolved as a signal of dominance, as this type of coloration is more common in species that live in social groups in the nonbreeding season, and species that nest in colonies; two socio-ecological conditions that promote frequent agonistic interactions with numerous and/or unfamiliar individuals. Additionally, our study suggests that carotenoid bill coloration is independent of the intensity of past sexual selection, as it is not related to either sexual dichromatism or sexual size dimorphism. These results pose a significant challenge to the conventional view that carotenoid-based avian coloration has evolved as a developmentally costly, condition-dependent sexual signal. We also suggest that bare part ornamentation may often signal different information than plumage ornaments.
Asunto(s)
Pico , Conducta Animal , Carotenoides/metabolismo , Pigmentación , Pájaros Cantores/fisiología , Animales , Tamaño Corporal , Plumas , Femenino , Masculino , Modelos Estadísticos , Caracteres Sexuales , Conducta Sexual , Conducta SocialRESUMEN
Despite significant improvements in systemic therapy for patients with colorectal liver metastases (crlms), response rates in the first-line setting are not optimal, and response rates in the second-line setting remain disappointing. Hepatic arterial infusion pump (haip) chemotherapy has been extensively studied in patients with crlms, but it remains infrequently used. We convened an expert panel to discuss the role of haip in the contemporary management of patients with crlm. Using a consensus process, we developed these statements: haip chemotherapy should be given in combination with systemic chemotherapy.haip chemotherapy should be offered in the context of a multidisciplinary program that includes expertise in hepatobiliary surgery, medical oncology, interventional radiology, nursing, and nuclear medicine.haip chemotherapy in combination with systemic therapy should be considered in patients with unresectable crlms who have progressed on first-line systemic treatment. In addition, haip chemotherapy is acceptable as first-line treatment in patients with unresectable colorectal liver metastases.haip chemotherapy is not recommended in the setting of extrahepatic disease outside the context of a clinical trial.haip chemotherapy in combination with systemic therapy is an option for select patients with resected colorectal liver metastases. These consensus statements provide a framework that clinicians who treat patients with crlm can use when considering treatment with haip.
RESUMEN
PURPOSE: Laryngotracheobronchial injuries (LTBI) are serious injuries because of their consequences in terms of ventilation, coupled with the severity of other injuries associated with them. We share our experience in managing these patients perioperatively in our level 1 trauma centre. METHODS: A retrospective analysis of the records of 30 patients with LTBI who presented at Jai Prakash Narayan Apex Trauma Center (JPNATC) from December 2007 to February 2011 was done. The demographics, mechanism of injury, clinical presentation, diagnostic modalities, anaesthetic management and outcome in these patients were reviewed. RESULTS: Intrathoracic location of the injury and Injury Severity Score (ISS) had a direct correlation with the outcome of the patients. The overall mortality was 6.7 %. CONCLUSION: Meticulous examination, details about the mechanism of injury, careful diagnostic evaluation, and skilful airway and surgical management are necessary for a better outcome in patients with airway injuries. A high degree of suspicion in occult injuries and liberal use of a fibreoptic bronchoscope aids diagnosis and management. Prompt airway management in the pre-hospital setting before transfer to a higher level trauma centre ensures better outcomes.
RESUMEN
Measuring von Willebrand factor (VWF) activity is essential to the diagnosis of von Willebrand disease (VWD). The VWF activity is usually assessed based on measurement of the ristocetin cofactor (VWF:RCo). However, that test is technically challenging and has high intra- and inter-assay variabilities. The HemosIL VWF activity (VWF:AC) is a fully automated assay, recently proposed as a good alternative to VWF:RCo for VWD diagnosis. This study was undertaken to assess this new method. First, the analytical performance of VWF:AC on an automated coagulo-meter (ACLTop) was determined, and then this new method was compared with VWF:RCo and the platelet function analyzer (PFA100) for 160 patients referred for VWD screening. The VWF:AC achieved acceptable precision with within-run and between-run coefficients of variation ranging from 2.3% to 14.1%, and linearity from 10% to 100%. Despite some marked differences between VWF:AC and VWF:RCo for 10 plasmas tested, their agreement for VWD diagnosis was good. The VWF:AC had sensitivity similar to that of PFA100 (close to 100%), but better specificity (97.7% vs. 66% or 60%, depending on the cartridge used). The good analytical performance, and the sensitivity and specificity of VWF:AC to detect VWF deficiency renders it a suitable method for VWD screening. Our findings support VWF:AC use for the diagnostic work-up of VWD, paying close attention to concomitant clinical signs and bleeding score, as recommended for VWD.
Asunto(s)
Pruebas de Coagulación Sanguínea/normas , Enfermedades de von Willebrand/diagnóstico , Factor de von Willebrand/análisis , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Área Bajo la Curva , Automatización , Pruebas de Coagulación Sanguínea/métodos , Niño , Preescolar , Femenino , Humanos , Lactante , Masculino , Persona de Mediana Edad , Curva ROC , Sensibilidad y Especificidad , Adulto JovenRESUMEN
Fish compensate for acid-base disturbances primarily by modulating the branchial excretion of acid-base equivalents, with a supporting role played by adjustment of urinary acid excretion. The present study used metabolic acid-base disturbances in rainbow trout, Oncorhynchus mykiss, to evaluate the role played by cortisol in stimulating compensatory responses. Trout infused with acid (an iso-osmotic solution of 70 mmol L(-1) HCl), base (140 mmol L(-1) NaHCO(3)) or saline (140 mmol L(-1) NaCl) for 24 h exhibited significant elevation of circulating cortisol concentrations. Acid infusion significantly increased both branchial (by 328 µmol kg(-1) h(-1)) and urinary (by 5.9 µmol kg(-1) h(-1)) net acid excretion, compensatory responses that were eliminated by pre-treatment of trout with the cortisol synthesis inhibitor metyrapone (2-methyl-1,2-di-3-pyridyl-1-propanone). The significant decrease in net acid excretion (equivalent to enhanced base excretion) of 203 µmol kg(-1) h(-1) detected in base-infused trout was unaffected by metyrapone treatment. Acid- and base-infusions also were associated with significant changes in the relative mRNA expression of branchial and renal cytosolic carbonic anhydrase (tCAc) and renal membrane-linked CA IV (tCA IV). Cortisol treatment caused changes in CA gene expression that tended to parallel those observed with acid but not base infusion. For example, significant increases in renal relative tCA IV mRNA expression were detected in both acid-infused (~2x) and cortisol-treated (~10x) trout, whereas tCA IV mRNA expression was significantly reduced (~5x) in base-infused fish. Despite changes in CA gene expression in acid- or base-infused fish, neither acid nor base infusion affected CAc protein levels in the gill, but both caused significant increases in branchial CA activity. Cortisol treatment similarly increased branchial CA activity in the absence of an effect on branchial CAc protein expression. Taken together, these findings provide support for the hypothesis that in rainbow trout, cortisol is involved in mediating acid-base compensatory responses to a metabolic acidosis, and that cortisol exerts its effects at least in part through modulation of CA.
Asunto(s)
Equilibrio Ácido-Base , Anhidrasas Carbónicas/metabolismo , Hidrocortisona/fisiología , Animales , Anhidrasas Carbónicas/genética , Expresión Génica/efectos de los fármacos , Ácido Clorhídrico/farmacología , Metirapona/farmacología , Oncorhynchus mykiss , Bicarbonato de Sodio/farmacología , Cloruro de Sodio/farmacologíaRESUMEN
The paper presents major lipid classes and their fatty acids investigated from Ascaridia galli, a nematode parasite of country fowl. Thin layer chromatography (TLC) reveals that the percent of total lipid, neutral lipid, phospholipids, and glycolipids are 1.94, 54.39, 26.95 and 18.66, respectively. Gas-liquid chromatography (GLC) analysis shows that the saturated fatty acids are the major components in all the lipid fractions followed by monoenes and dienes. Polyunsaturated fatty acids (PUFA) were present in low amount. Stearic acids (C(18)) were the chief components among all the fatty acids in all the lipid fractions.
RESUMEN
Unconscious patients are frequently referred to radiology departments for computed tomography (CT) of the brain. The objectives of these examinations are to define the underlying cause of impaired consciousness and in some cases to determine the severity of associated brain injury. There is often little history available to guide the clinician or radiologist, particularly in patients referred from the casualty department. In this review, we present the typical CT appearances of adult patients presenting with loss of consciousness for CT examination. We focus on the most common abnormalities that are identified in everyday radiological practice, emphasize important diagnostic signs that may enable a confident diagnosis to be made and discuss when further imaging may be warranted.
Asunto(s)
Encéfalo/diagnóstico por imagen , Tomografía Computarizada por Rayos X/métodos , Inconsciencia/diagnóstico por imagen , Adulto , Infecciones del Sistema Nervioso Central/diagnóstico por imagen , Hematoma/diagnóstico por imagen , Humanos , Hipoxia-Isquemia Encefálica/diagnóstico por imagen , Hemorragias Intracraneales/diagnóstico por imagen , Hipertensión Intracraneal/diagnóstico por imagen , Tromboembolia/diagnóstico por imagen , Inconsciencia/complicacionesRESUMEN
AIMS/HYPOTHESIS: On the basis of our previous studies, we investigated the possible role of focal adhesion kinase (FAK) in the development of insulin resistance in skeletal muscle, a major organ responsible for insulin-stimulated glucose uptake. MATERIALS AND METHODS: Insulin-resistant C2C12 skeletal muscle cells were transfected with FAK wild-type or FAK mutant plasmids, knocked down using small interfering RNA (siRNA), and their effects on the levels and activities of insulin-signalling molecules and on glucose uptake were determined. RESULTS: A significant decrease in tyrosine phosphorylation of FAK in insulin-resistant C2C12 cells was observed. A similar decrease was observed in skeletal muscle obtained from insulin-resistant Sprague-Dawley rats fed a high-fat diet. Increased levels of FAK in insulin-resistant C2C12 skeletal muscle cells increased insulin sensitivity and glucose uptake. These effects were reversed by an increase in the level of kinase activity mutant FAK or suppression of endogenous FAK by siRNA. FAK was also found to interact downstream with insulin receptor substrate-1, phosphatidylinositol 3-kinase and protein kinase C and glycogen synthase kinase 3beta, leading to translocation of glucose transporter 4 and resulting in the regulation of glucose uptake. CONCLUSIONS/INTERPRETATION: The present study provides strong evidence that the modulation of FAK level regulates the insulin sensitivity of skeletal muscle cells. The results demonstrate a direct role of FAK in insulin-resistant skeletal muscle cells for the first time.
Asunto(s)
Proteína-Tirosina Quinasas de Adhesión Focal/fisiología , Resistencia a la Insulina/fisiología , Músculo Esquelético/fisiología , Animales , Línea Celular , Proteína-Tirosina Quinasas de Adhesión Focal/genética , Cinética , Ratones , Fosfatidilinositol 3-Quinasas/metabolismo , Fosforilación , ARN Interferente Pequeño/genética , TransfecciónRESUMEN
BACKGROUND: Relationships between income inequality and various health indicators have been the subject of much study and some controversy. We investigated associations between child mortality and income inequality amongst the wealthier OECD countries as well as changes in their relative child mortality rankings over time. METHODS: Data were drawn from the 2003-2006 'State of the World's Children' reports published by UNICEF; Gini coefficients on income inequality were also used. Pearson correlation coefficients were calculated to investigate associations. Longitudinal child mortality data was used to compare rankings. RESULTS: We discovered very strong associations between child mortality and income inequality. In contrast to earlier results, these associations persist when the USA is excluded from the analysis. The countries with the worst child mortality figures were previously singled out in a 1993 UNICEF study on child neglect in rich nations. We also report their worsening child mortality rankings, since 1960, relative to the other OECD countries. CONCLUSIONS: The results strengthen the existing evidence linking child mortality with income inequality in wealthy nations, and add to the evidence that sociopolitical factors are important in this regard.
Asunto(s)
Mortalidad del Niño , Renta/estadística & datos numéricos , Preescolar , Países Desarrollados , Países en Desarrollo , Humanos , Lactante , Recién Nacido , Política , Naciones UnidasRESUMEN
AIMS/HYPOTHESIS: Miltefosine, the first oral anti-leishmanial drug, is reported to inhibit phosphatidylinositol 3-kinase (PI3K)/Akt activity in carcinoma cell lines. Inhibition of the PI3K/Akt pathway is known to result in insulin resistance. Therefore, we investigated whether miltefosine has any deleterious effect(s) on insulin sensitivity in L6E9 skeletal muscle cells. MATERIALS AND METHODS: L6E9 myotubes were treated with miltefosine and its effect was observed on insulin-signalling proteins such as Akt, PI3K, insulin receptor-beta, IRS-1, c-Jun N-terminal kinase, p38 and glycogen synthase kinase beta, as well as on glucose uptake. RESULTS: Miltefosine caused skeletal muscle insulin resistance in vitro by interfering with the insulin-signalling pathway and inhibiting insulin-stimulated glucose uptake. CONCLUSIONS/INTERPRETATION: Miltefosine may contribute to the risk of type 2 diabetes and needs further clinical exploration.
Asunto(s)
Resistencia a la Insulina , Fibras Musculares Esqueléticas/efectos de los fármacos , Fosforilcolina/análogos & derivados , Animales , Antiprotozoarios/efectos adversos , Células Cultivadas , Evaluación Preclínica de Medicamentos , Glucosa/metabolismo , Insulina/farmacología , Fibras Musculares Esqueléticas/metabolismo , Proteína Oncogénica v-akt/metabolismo , Fosforilcolina/efectos adversos , RatasRESUMEN
The present study focuses on the physical and chemical stability of insulin under iontophoretic conditions using HPLC, SDS-PAGE, RIA and biological assay. Influence of pH, concentration of insulin, current strength and duration of current application on the stability of insulin was studied. Anodal iontophoresis at pH 7.4 caused more than 80% degradation of insulin, while the degradation was minimal at pH 3.6. The degradation was not influenced by insulin concentration, but increase in current strength above 0.75 mA/cm2 or application of current for 12 h (at 0.5 mA/cm2) led to 80 and 20% degradation respectively. All the samples showed biological activity comparable to intact insulin.
Asunto(s)
Hipoglucemiantes/química , Insulina/química , Administración Cutánea , Animales , Glucemia/metabolismo , Fenómenos Químicos , Química Física , Cromatografía Líquida de Alta Presión , Diabetes Mellitus Experimental/sangre , Diabetes Mellitus Experimental/tratamiento farmacológico , Cámaras de Difusión de Cultivos , Electroquímica , Electroforesis en Gel de Poliacrilamida , Humanos , Concentración de Iones de Hidrógeno , Hipoglucemiantes/administración & dosificación , Hipoglucemiantes/uso terapéutico , Insulina/administración & dosificación , Insulina/uso terapéutico , Iontoforesis , Cinética , Radioinmunoensayo , Ratas , Ratas Sprague-Dawley , Espectroscopía Infrarroja por Transformada de FourierRESUMEN
In this study the anti-leishmanial activity and anti-microtubule effects of paclitaxel, trifluralin and a combination of paclitaxel and trifluralin have been tested in a wild type and sodium arsenite-resistant strain of Leishmania donovani. Both paclitaxel and trifluralin have been shown to be effective in limiting parasite growth. Specific alterations in morphology, tubulin polymerization dynamics, post-translational modifications and cellular distribution of the tubulins have been confirmed to be a part of the intracellular anti-microtubule-events that occur in arsenite-resistant L. donovani in response to these agents, ultimately leading to death of the parasite. DNA analyses of the drug-treated wild type and arsenite-resistant strains revealed an apoptosis-like death in response to paclitaxel and the combination but not to trifluralin. Data provide valuable information for further development of chemotherapeutic strategies based on anti-microtubule agents against drug resistant Leishmania parasites.
Asunto(s)
Antiprotozoarios/farmacología , Resistencia a Medicamentos/fisiología , Leishmania donovani/efectos de los fármacos , Leishmania donovani/metabolismo , Tubulina (Proteína)/metabolismo , Animales , Apoptosis/efectos de los fármacos , Arsenitos/farmacología , Fragmentación del ADN , Quimioterapia Combinada , Expresión Génica/efectos de los fármacos , Paclitaxel/farmacología , Procesamiento Proteico-Postraduccional , Compuestos de Sodio/farmacología , Trifluralina/farmacología , Tubulina (Proteína)/efectos de los fármacosRESUMEN
Traumatic hip dislocation in the paediatric population is a relatively rare occurrence and constitutes an orthopaedic emergency. A trivial force is all that is required and non-accidental injury should not be necessarily suspected. A case report involving a hip dislocation in a 21-month-old child, the youngest in the recent English literature is detailed.