Cadmium (Cd) contamination in cropland poses a significant threat to the quality of agricultural products, but even though in-situ remediation has been extensively applied, non-selective immobilization remains an issue. In order to develop a material that specifically immobilizes Cd in soil, a layered double hydroxide, intercalated with mercaptosuccinic acid (MSA-CFA), was synthesized through co-precipitation. In this case, the MSA-CFA's maximum adsorption capacity was increased from the 513.8â¯mg·g-1 for unintercalated hydrotalcite CFA to 692.6â¯mg·g-1. Besides, MSA-CFA efficiently removed 99.25â¯% of Cd from soil water-extract solution and immobilized up to 70.03â¯% of bio-available Cd. However, interestingly, its immobilization effects on beneficial metal elements Fe, Mn and Zn were milder, being equivalent to 2/7, 5/7 and 1/2 that of lime, respectively. Moreover, XRD and XPS techniques revealed isomorphous substitution with calcium and sulfhydryl complexation during the Cd adsorption by MSA-CFA. Compared with CFA, the increased adsorption capacity of MSA-CFA for Cd was due to intercalated MSA acting as a new adsorption site, while the enhanced selectivity was contributed by sulfhydryl's affinity for Cd. Altogether, MSA-CFA showed great promise as a competitive and highly efficient candidate amendment in Cd-contaminated soil remediation.
The establishment of ecological risk thresholds for arsenic (As) plays a pivotal role in developing soil conservation strategies. However, despite many studies regarding the toxicological profile of As, such thresholds varying by diverse soil properties have rarely been established. This study aims to address this gap by compiling and critically examining an extensive dataset of As toxicity data sourced from existing literature. Furthermore, to augment the existing information, experimental studies on As toxicity focusing on barley-root elongation were carried out across various soil types. The As concentrations varied from 12.01 to 437.25 mg/kg for the effective concentrations that inhibited 10% of barley-root growth (EC10). The present study applied a machine-learning approach to investigate the complex associations between the toxicity thresholds of As and diverse soil properties. The results revealed that Mn-/Fe-ox and clay content emerged as the most influential factors in predicting the EC10 contribution. Additionally, by using a species sensitivity distribution model and toxicity data from 21 different species, the hazardous concentration for x% of species (HCx) was calculated for four representative soil scenarios. The HC5 values for acidic, neutral, alkaline, and alkaline calcareous soils were 80, 47, 40, and 28 mg/kg, respectively. This study establishes an evidence-based methodology for deriving soil-specific guidance concerning As toxicity thresholds.
The accumulation of antimony (Sb) in plants and its potential effects on human health are of increasing concern. Nevertheless, only a few countries or regions have established soil Sb thresholds for agricultural purposes, and soil properties have not been taken into account. This study investigated the accumulation of Sb in the edible parts of pakchoi and wheat grain by adding exogenous Sb to 21 soils with varying properties. The results revealed a positive correlation between bioavailable Sb (Sbava, extracted by 0.1 M K2HPO4) in soil and Sb in the edible parts of pakchoi (R2 = 0.77, p < 0.05) and wheat grain (R2 = 0.54, p < 0.05). Both machine learning and traditional multiple regression analysis indicated Sbava was the most critical feature and the main soil properties that contributed to Sb uptake by pakchoi and wheat were CaCO3 and clay, respectively. The advisory food limits for Sb in pakchoi and wheat were estimated based on health risk assessment, and used to derive soil thresholds for safe pakchoi and wheat production based on Sbtot and Sbava, respectively. These findings hold potential for predicting Sb uptake by crops with different soil properties and informing safe production management strategies.
The biogeochemical cycling of iron (Fe) or sulfur (S) in paddy soil influences the cadmium (Cd) and arsenic (As) migration. However, the influence of coupled reduction effects and reaction precedence of Fe and S on the bioavailability of Cd and As is still not fully understood. This study aimed to reveal the influence of Fe and S reduction on soil Cd and As mobility under various pe + pH conditions and to elucidate the related mechanism in subtropical China. According to the findings, higher adsorption from Fe reduction caused high-crystalline goethite (pe + pH > 2.80) to become amorphous ferrihydrite, which in turn caused water-soluble Cd (62.0%) to first decrease. Cd was further decreased by 72.7% as a result of the transformation of SO42- to HS-/S2- via sulfate reduction and the formation of CdS and FeS. As release (an increase of 8.1 times) was consequently caused by the initial reduction and dissolution of iron oxide (pe + pH > 2.80). FeS had a lesser impact on the immobilization of As than sulfate-mediated As (V) reduction in the latter stages of the reduction process (pe + pH < 2.80). pe + pH values between 3 and 3.5 should be maintained to minimize the bioavailability of As and Cd in moderate to mildly polluted soil without adding iron oxides and sulfate amendments. The practical remediation of severely co-contaminated paddy soil can be effectively achieved by using Fe and S additions at different pe + pH conditions. This technique shows promise in reducing the bioavailability of Cd and As.
In developing countries, like Pakistan, the pursuit of urbanization and economic development disrupts the delicate ecosystem, resulting in additional biogeochemical emissions of heavy metals into the human habitat and posing significant health risks. The levels of these trace elements in humans remain unknown in areas at higher risk of pollution in Pakistan. In this investigation, selected trace metals including Copper (Cu), Chromium (Cr), Lead (Pb) Cadmium (Cd), Cobalt (Co), Nickel (Ni), and Arsenic (As) were examined in human hair, urine, and nail samples of different age groups from three major cities (Muzaffargarh, Multan, and Vehari) in Punjab province, Pakistan. The results revealed that the mean concentrations (ppm) of Cr (1.1) and Cu (9.1) in hair was highest in Muzaffargarh. In urine samples, the mean concentrations (µg/L) of Co (93), As (79), Cu (69), Cr (56), Ni (49), Cd (45), and Pb (35) were highest in the Multan region, while As (34) and Cr (26) were highest in Vehari. The mean concentrations (ppm) of Ni (9.2), Cr (5.6), and Pb (2.8), in nail samples were highest in Vehari; however, Multan had the highest Cu (28) concentration (ppm). In urine samples, the concentrations of all the studied metals were within permissible limits except for As (34 µg/L) and Cr (26 µg/L) in Vehari. However, in nail samples, the concentrations of Ni in Multan (8.1 ppm), Muzaffargarh (9 ppm), Vehari (9.2 ppm), and Cd (3.69 ppm) in Muzaffargarh exceeded permissible limits. Overall, the concentrations of metals in urine, nail, and hair samples were higher in adults (39-45 age group). Cr, Cu, and Ni revealed significantly higher concentrations of metals in hair and water in Multan, whereas As in water was significantly (p < 0.001) correlated with urinary As in Multan, indicating that the exposure source was region-specific.
It is urgent to detect the major controlling factors and establish predictive models of mercury (Hg) accumulation in rice. A pot trial was conducted, exogenous Hg was added to 19 paddy soils at 4 concentration levels in this study. The major controlling factors of total Hg (THg) in brown rice were soil THg, pH and organic matter (OM) content, while those of methylmercury (MeHg) in brown rice were soil MeHg and OM. THg and MeHg in brown rice could be well predicted by soil THg, pH and clay content. The data from previous studies were collected to validate the predictive models of Hg in brown rice. The predicted values of Hg in brown rice were within the twofold prediction intervals of the observations, which demonstrated the predictive models in this study were reliable. The results could provide theoretical foundation for the risk assessment of Hg in paddy soils.
Mercury , Methylmercury Compounds , Oryza , Soil Pollutants , Methylmercury Compounds/chemistry , Mercury/analysis , Oryza/chemistry , Environmental Monitoring , Soil Pollutants/analysis , Soil/chemistry
By studying the dynamic characteristics of and key growth stages for mercury (Hg) enrichment in rice, the Hg migration and translocation processes in this species can be better understood. In this study, a pot experiment was conducted, wherein two rice cultivars, Tianyouhuazhan (TYHZ, indica) and Zhendao 18 (ZD18, japonica), were selected and planted for analysing the Hg accumulation kinetic characteristics in rice plants. The plants were sampled at each growth stage, and the biomass and total Hg (THg) and methylmercury (MeHg) concentrations of each tissue were measured. The relative Hg contribution rates (CRs) in whole rice plants and rice grains were calculated, and the growth stage with the highest relative contribution was identified as the key growth stage for Hg accumulation. The results indicated that in rice, the MeHg translocation capability was stronger than the THg translocation capability. Significant differences in the kinetic characteristics of Hg accumulation were found between the two rice cultivars, and the TYHZ rice grains had a stronger Hg accumulation ability than the ZD18 rice grains. The key growth stages for THg accumulation in whole rice plants of both cultivars were the tillering and booting stages, while that for MeHg accumulation was the tillering stage. The key period for Hg accumulation in rice grains was the grain filling stage for both cultivars. The insights from this study could provide scientific guidance for the safe production of rice in Hg-contaminated soil.
Mercury , Methylmercury Compounds , Oryza , Soil Pollutants , Mercury/analysis , Soil , Soil Pollutants/analysis , Environmental Monitoring , Methylmercury Compounds/analysis
In order to understand the differences in the uptake and accumulation of several common exogenous selenium fertilizers by crops, a wheat pot experiment was conducted to study the availability changes in different selenium fertilizers (potassium selenate, potassium selenite, EDTA-chelated selenium, selenium powder, fly ash, and selenium-enriched straw) in soil and their effects on wheat growth and selenium uptake and distribution. The results showed that the change in availability of different exogenous selenium types in soil was different. During the whole growth period of wheat, the soil available selenium proportion of selenate, selenite, and EDTA-chelated selenium treatment was significantly higher than that of the control (CK), respectively, but there was no significant difference between the other treatments and the CK treatment. In the early stage of wheat growth, the soil available selenium proportion of selenate, selenite, and selenium powder treatment decreased gradually and tended to be stable in the later growth stage of wheat; however, the soil available selenium proportion of other exogenous selenium treatments showed a dynamic change of decreasing in the early period and increasing in the late period. The available selenium content in soil significantly affected the selenium uptake by wheat, and there was a significant positive correlation between them. Selenate application significantly increased the grain and leaf biomass of wheat, but other selenium fertilizers had no significant effect on wheat growth. The accumulation capacity of different exogenous selenium fertilizers for wheat followed the order of selenate>selenite, EDTA-chelated selenium>selenium powder, fly ash, and selenium-enriched straw. There was no significant difference between the selenium powder, fly ash, and selenium-enriched straw treatments and the CK treatment. Selenium was more easily transferred to and accumulated in the stems and leaves of wheat after the application of selenate, whereas selenium was more easily transferred to and accumulated in grains after the application of selenite and EDTA-chelated selenium.
Selenium , Soil , Selenic Acid , Triticum , Fertilizers/analysis , Coal Ash , Edetic Acid , Powders , Selenious Acid
Antibiotic resistance gene (ARG)-contaminated food from manure application is gaining widespread interest, but little is known about the distribution and uptake of ARGs in peanuts that are subjected to manure routinely. In this study, the ARG profile and bacterial community in soil and peanut plants from a 7-year manure-fertilized field were investigated using high-throughput qPCR and 16S rRNA gene sequencing. Manure application increased the abundance of ARGs in soil and peanuts by 59-72 and 4-10 fold, respectively. The abundance of ARGs from high to low was as follows: manure, shell-sphere soil, rhizosphere soil, bulk soil, stems, shells, needles, kernels, and roots. Source-tracker analyses were used to investigate the potential source of ARGs in peanut kernels, which revealed that the ARGs in peanut kernels may be primarily absorbed by the roots from the soil. The horizontal gene transfer (HGT) of ARGs was the primary factor in the spread of ARGs, and Proteobacteria were the primary agents of HGT between different parts of peanut plants. Additionally, norank_Chloroplast from the phylum Cyanobacteria was the most important contributor to the abundance of ARGs in peanut kernels. Overall, our findings fill a gap in our understanding of the distribution patterns of ARGs in peanut plants and the migratory pathways of ARGs from soil to peanut kernels.
Anti-Bacterial Agents , Soil , Anti-Bacterial Agents/pharmacology , Arachis , Genes, Bacterial , Manure/analysis , RNA, Ribosomal, 16S/genetics , Soil Microbiology , Drug Resistance, Microbial/genetics
Antibiotic-degrading bacteria are commonly used to treat antibiotic contamination, but the antibiotic resistance genes (ARGs) they carry are often overlooked. This study used metagenomic assembly and binning analyses to explore potential antibiotic-degrading bacteria and their ARGs during pig manure composting. The result showed that 35 metagenome-assembled genomes (MAGs) mainly containing alkyl-aryl transferase and decarboxylase genes involved in the removal of antibiotics. Multidrug (124), ß-lactam (67), macrolide-lincosamide-streptogramin (MLS) (64), and tetracycline (43) were the central ARG types detected in the 35 MAGs. Furthermore, the risk of ARGs was evaluated using the arg_ranker framework, and 19 MAGs were found to contain intermediate-high-risk ARGs with human-associated-enrichment, gene transferability, and host pathogenicity. Bin 34 of the genus of Geofilum had the highest ARG risk. Bin 6, Bin 11 and Bin 14 of the genus of Limnochorda, Chelatococcus and Niabella, had a lower ARG risk and were considered as potential antibiotic-degrading bacteria.
Composting , Manure , Swine , Humans , Animals , Manure/microbiology , Genes, Bacterial/genetics , Metagenome , Anti-Bacterial Agents/pharmacology , Bacteria/genetics , Drug Resistance, Microbial/genetics
The rising atmospheric CO2 is a major driver for climate change, directly affects rice production. Cadmium (Cd) in paddy soils also serves as a persistent concern. Currently, few studies consider the rice response to coupled stresses of elevated CO2 (eCO2) and soil Cd. Experimental evidence understanding the effects and mechanisms of eCO2 on Cd uptake by rice is lacking yet. In a free-air CO2 enrichment (FACE) system, a 3-year pot experiment was conducted to explore the Cd uptake by rice under two CO2 conditions (ambient and ambient + 200 µmol·mol-1) using combinations of in-situ Cd-contaminated soils and associated rice varieties. Results showed that more low-crystalline Fe oxides (Feh) in iron plaque (IP) were deposited on root surface with the increased dissolved Fe2+ due to lower soil redox status under eCO2. The Cd accumulation in rice was hindered due to more Cd associated with Feh (Feh-Cd) rather than uptake by roots. Taken together, the relative effects of eCO2 on Cd uptake by rice were consistent across years under different Cd-contaminated soils. Our findings will help to better understand the Cd uptake by rice under future climate conditions, and thus push the development of climate-crop-soil models and accurate prediction for food security.
Oryza , Soil Pollutants , Cadmium/chemistry , Oryza/chemistry , Carbon Dioxide , Soil Pollutants/analysis , Soil/chemistry , Iron/chemistry , Oxides
The increasing One-Health concept calls for a more in-depth understanding of the dissemination of antibiotic resistance in plant microbiomes. While there is considerable published evidence that microplastics can promote the spread of antibiotic resistance genes (ARGs) in the environment, whether and how microplastics impact the plant endophytic resistome are largely unknown. Here we examined the ARGs along the soil-root continuum of maize and wheat under the pressure of microplastics. Amendment with heavy metals was also included as they can apply the selective pressure for ARG spread as well. The crop species and genotypes had significant effects on the root endophytic ARG abundance and diversity. The greatest ARG abundance was observed in the maize ZD958 endophytes (0.215 copies per 16S rRNA gene), followed by the maize XY335 (0.092 copies per 16S rRNA gene). For each crop genotype, amendment with microplastics and heavy metals significantly increased the ARG abundances and changed their profiles in root endophytes. The endophytic ARG variances were closely associated with the endophytic microbiome, the rhizosphere bacterial communities and resistome. Additionally, the level of endophytic ARGs was positively relevant to the abundance of mobile genetic elements (MGEs). These findings suggested that the root endophytic resistome was primarily affected by the crop species, and microplastics might show enhancement effects on the endophytic resistome via changing the root-associated microbiome and facilitating the MGE mediation. Overall, this study, for the first time, highlights the root endophytic ARG emergence and dissemination induced by microplastics.
Anti-Bacterial Agents , Microplastics , Anti-Bacterial Agents/pharmacology , RNA, Ribosomal, 16S/genetics , Plastics , Genes, Bacterial , Soil , Endophytes
Biodegradable plastics have emerged as an ecological alternative to conventional petroleum-based plastics. Despite the recent advances in the effects of conventional microplastic on soil ecosystems, the ecological impact of biodegradable microplastics in soil environments remains poorly understood. Here, we performed soil microcosms with conventional (polyethylene and polystyrene) and biodegradable (polybutylene succinate and polylactic acid) microplastics to estimate their effects on the success patterns, co-occurrence networks, and the assembly mechanisms of soil bacterial communities. Biodegradable microplastics significantly altered the soil bacterial community composition with steeper temporal turnovers (rate: 0.317 - 0.514) compared to the conventional microplastic treatments (rate: 0.211 - 0.220). Network under biodegradable microplastics showed greater network complexity, including network size, connectivity, average clustering coefficient, and the number of keystone species, as compared with the conventional microplastic treatments. Additionally, the biodegradable microplastic network had higher robustness, which may be potentially due to the enhanced dissolved organic carbon contents in the soil treated with biodegradable microplastics. The bacterial community assembly was initially governed by deterministic homogeneous selection (93 - 100 %) under the stress of microplastics, but was progressively structured by increasing stochastic homogeneous dispersal (17.8 - 73.3 %) over time. The normalized stochasticity ratio also revealed that the application of microplastics increased the importance of stochastic processes following incubation. These findings greatly enhanced our understanding of the ecological mechanisms and interactions of soil bacterial communities in response to microplastic stress.
Biodegradable Plastics , Microplastics , Bacteria , Ecosystem , Microplastics/toxicity , Plastics/toxicity , Soil , Soil Microbiology
The gradual accumulation of microplastics has aroused increasing concern for the unique niche, termed "plastisphere." As research so far has focused on their characteristics in aquatic ecosystems, our understanding of the colonization and assembly of the attached bacterial communities on microplastics in soil ecosystems remains poor. Here, we aimed to characterize the plastisphere microbiomes of two types of microplastics (polylactic acid [PLA] and polyethylene [PE]) differing in their biodegradability in two different soils. After incubation for 60 days, considerably lower alpha diversity of bacterial community was observed on the microplastic surfaces, and prominent divergences occurred in the microbial community compositions between the plastisphere and the bulk soil. The temperature, rather than polymer type, significantly induced the differences between the plastisphere communities. The rRNA gene operon (rrn) copy numbers were significantly higher in the PLA plastisphere, suggesting potential degradation. The co-occurrence network analysis showed that the PE plastisphere exhibited greater network complexity and stronger stability than those in the PLA plastisphere. The stochasticity ratio indicated the remarkable importance of stochastic process on community assembly in PE and PLA plastispheres, while the null model analysis showed the nonnegligible roles of deterministic processes in shaping the plastisphere communities. Higher contributions of homogenous selection in the PLA plastisphere were observed in comparison with the PE plastisphere, which could probably be attributed to the selective pressure induced by microplastic degradation. Our findings enhance our mechanistic understanding of the diversity patterns and assembly processes of plastisphere in soil environments and have important implications for microbial ecology and microplastic risk assessment. IMPORTANCE The increasing pervasive microplastic pollution is creating a new environmental compartment, termed plastisphere. Even though there was conclusive information characterizing the plastisphere, the underlying mechanisms shaping the bacterial communities in the plastisphere in the soil remain unclear. Therefore, we incubated two types of microplastics (PE and PLA) in two different soils and explored the differences between plastisphere and bulk soil communities. Additionally, the co-occurrence network and the assembly processes of plastisphere were subjected to further analysis. Our results highlight the importance of selective recruitment of microplastics and contribute to the understanding of the diversity patterns and assembly processes of plastisphere in soil environments.
Microbiota , Microplastics , Plastics , Soil , Soil Microbiology , Polyesters , Microbiota/genetics , Polyethylene
Temperature variation can have a significant impact on arsenic (As) bioavailability in paddy soils. However, details regarding the transformation of exogenous As during the aging process in paddy soils at various temperatures remain unclear. This work investigated the effects of temperature on the As extractability and As species transformation of three paddy soils spiked with exogenous arsenate at 60 mg kg-1 under flooded aging and explored the related chemical and microbial mechanisms. The results showed that 0.05 M NH4H2PO4-extractable As decreased over time during flooded aging for 192 days, and it decreased by approximately one-third at 35 °C compared with 15 °C and 25 °C at the same aging time, indicating that higher temperatures facilitated the decrease in As extractability. As(V) reduction mainly occurred at 35 °C because the abundance and As(V)-reducing capacity of the predominant indigenous bacteria, the Bacillus sp strains, and the abundance of the arrA gene were significantly higher than those at 15 °C and 25 °C. The reduction of As(V) to As(III) and aging occurred simultaneously. The kinetic models were established, and the rate constants of the reduction and aging processes were obtained. Soil properties significantly affected the aging and reduction processes of extractable As(V). Our study indicated that elevating temperature had dual effects on the environmental risk of As in the flooded aging process. The previous definition of "aging" based on cationic metals needs to be updated according to the transformation characteristics of As species in flooded conditions. Our results addressed the necessity of impeding the reduction of As(V) in paddy soils under global warming.
Arsenic , Oryza , Soil Pollutants , Arsenic/chemistry , Soil/chemistry , Soil Pollutants/analysis , Temperature
Concerns about the ecological safety of both conventional and biodegradable microplastics have grown due to the inadequate end-of-life treatments of plastics. In this study, the effects of conventional and biodegradable microplastics on the spread of antibiotic resistance genes (ARGs) and virulence factors (VFs) were estimated in a soil microcosm experiment. The gene profiles and their respective bacterial hosts in soil were evaluated by metagenomic sequencing methods. The abundances of ARGs and VFs in polybutylene succinate (PBS) treated soils were statistically higher than the values in the control and conventional microplastic treatments. In comparison with the control, application of conventional microplastics showed negligible effects on ARG and VF profiles in the soil, while biodegradable microplastic amendments significantly changed the compositions of ARGs and VFs. The host-tracking analysis suggested application of microplastics broadened the bacterial hosts of ARGs and VFs in the soil. The percentage of Proteobacteria as ARG hosts increased from 38.5% in the control soils to 58.2% in microplastic exposed soil. The genus Bradyrhizobium was the dominant host of ARGs and VFs in biodegradable microplastic treatments, while conventional microplastics increased the percentages of Pseudomonas as the bacterial hosts. This study enhances the understanding of the effects of conventional and biodegradable microplastics on the propagation and hosts of ARGs and VFs in the terrestrial environment, providing essential insights into the risk assessment and management of plastics.
Microplastics , Plastics , Anti-Bacterial Agents/pharmacology , Bacteria/genetics , Drug Resistance, Microbial/genetics , Genes, Bacterial , Soil , Soil Microbiology , Virulence Factors
To identify the key microbial clusters and influencing factors involved in antibiotic removal from engineered anaerobic digestion (AD) systems, the dynamic characteristics of antibiotics, physiochemical factors, microbial communities and functional genes were investigated by 16S rRNA and metagenome sequencing. The results showed that antibiotic removal occurred mainly in the first 21 days, and sulfonamides had the highest removal rate. The key microbial clusters related to the biodegradation of antibiotics consisted mainly of Firmicutes and Bacteroidetes. The key enzymes consisted of deaminases, peptidases, C-N ligases, decarboxylases and alkyl-aryl transferases. Structural equation modelling indicated that low concentrations of propionic acid promoted the biodegradation activities of key microbial clusters in the first 21 days, but their activities were inhibited by the accumulated propionic acid after 21 days. Thus, propionic acid should be regulated in engineered AD systems to prevent the adverse effect of acid inhibition on antibiotic-degrading bacteria.
Anti-Bacterial Agents , Bacteria , Anaerobiosis , Anti-Bacterial Agents/pharmacology , Bacteria/metabolism , Biodegradation, Environmental , Metagenome , RNA, Ribosomal, 16S/genetics , RNA, Ribosomal, 16S/metabolism
To identify the key hosts involved in horizontal gene transfer (HGT) and vertical gene transfer (VGT) of antibiotic resistance genes (ARGs) and to determine the extent to and ways in which environmental properties contribute to ARG removal, the changes in ARG profile and key hosts during biogas residue and pig manure composting were investigated using metagenomic sequencing coupled with network analysis. Composting significantly reduced the abundances of ARGs other than bacA. Seventy and 41 hosts from Firmicutes, Actinobacteria, Proteobacteria and Bacteroidetes were associated with HGT and VGT, respectively. The key environmental properties were determined using structural equation modelling. Antibiotics directly affected HGT and determined ARG removal. Temperature indirectly affected HGT, mainly by influencing the degradation of antibiotics. BacA was associated only with hosts involved in VGT, which may lead to its low removal rate. These findings specify the priority and pathway of antibiotics and temperature affecting ARG profile.
Composting , Animals , Anti-Bacterial Agents/pharmacology , Drug Resistance, Microbial/genetics , Genes, Bacterial/genetics , Manure , Swine
Environmental concerns with liberal petroleum-based plastic use have led to demand for sustainable biodegradable alternatives. However, the inadequate end-of-life treatment of plastics may emit microplastics, either conventional or biodegradable, to the terrestrial environment. It is essential to evaluate the possible effects of conventional and biodegradable microplastics on the composition and function of soil microbial communities. Therefore, we conducted a soil microcosm experiment with polyethylene (PE), polystyrene (PS), polylactide (PLA), or polybutylene succinate (PBS) microplastics. The soil microbiome and metabolome were evaluated via 16S rRNA gene sequencing, metagenomics, and untargeted metabolomics. We reported that the presence of conventional or biodegradable microplastics can significantly alter soil microbial community composition. Compared to the control soils, the microbiome in PBS and PLA amended soils exhibited higher potential for uptake of exogenous carbohydrates and amino acids, but a reduced capacity for related metabolic function, potentially due to catabolite repression. No differences in soil metabolome can be observed between conventional microplastic treatments and the control. The potential reason may be that the functional diversity was unaffected by PE and PS microplastics, while the biodegradable particles promoted the soil microbial multifunctionality. Our findings systematically shed light on the influence of conventional and biodegradable microplastics on soil microorganisms, facilitating microplastic regulation.
Microbiota , Microplastics , Metabolome , Plastics , RNA, Ribosomal, 16S/genetics , Soil
A growing body of evidence suggests that microplastics may be colonized with a unique microbiome, termed 'plastisphere', in aquatic environments. However, the deep mechanisms (deterministic and/or stochastic processes) underlying the community assembly on microplastics are still poorly understood. Here, we took the estuary of Hangzhou Bay (Zhejiang, China) as an example and examined the assembly mechanisms of bacterial communities in water and microplastic samples. Results from high-throughput sequencing showed that Proteobacteria, Firmicutes, and Actinobacteria were the dominant phyla across all samples. Additionally, microorganisms from plastisphere and planktonic communities exhibited contrasting taxonomic compositions, with greater within-group variation for microplastic samples. The null model analysis indicated the plastisphere bacterial communities were dominantly driven by the stochastic process of drift (58.34%) and dispersal limitation (23.41%). The normalized stochasticity ratio (NST) also showed that the community assembly on microplastics was more stochastic (NST > 50%). Based on the Sloan neutral community model, the migration rate for plastisphere communities (0.015) was significantly lower than that for planktonic communities (0.936), potentially suggesting that it is the stochastic balance between loss and gain of bacteria (e.g., stochastic births and deaths) critically shaping the community assembly on microplastics and generating the specific niches. This study greatly enhanced our understanding of the ecological patterns of microplastic-associated microbial communities in aquatic environments.
Microbiota , Microplastics , Bacteria/genetics , Plastics , Stochastic Processes
