Plasma lipid levels of rats fed a diet containing pork fat as a source of lipids after splenic surgery.

Experimental studies have suggested an important role of the spleen in lipid metabolism, although with controversial results. Our purpose was to analyze the effect of a nutritionally balanced (NB) diet and a diet containing pork fat (PF) as source of lipids on the lipid profile of rats submitted to splenic surgery. Sixty adult male Wistar rats were divided into six groups of 10 animals each: 1 sham-operated, NB diet; 2 sham-operated, PF diet; 3 total splenectomy (TS), NB diet; 4 TS, PF diet; 5 TS followed by splenic autotransplantation (SA), NB diet; and 6 SA, PF diet. Blood samples were collected at the beginning (D0) and after 12 weeks of the experiment (D + 12) for plasma lipid determination. Morphologic regeneration of splenic tissues was observed, with no differences between groups 5 and 6. When D + 12 plasma lipid levels were compared to D0 levels there were no differences in groups 1, 3, and 5, while in groups 2, 4, and 6 total cholesterol (TC), low density lipoprotein (LDL), very low density lipoprotein (VLDL), and triacylglycerols (TAG) increased, and high density lipoprotein (HDL) decreased. At D + 12, groups 2, 4, and 6 had lower HDL than group 3. In conclusion, regardless of the surgical procedure applied to the spleen, an NB diet maintained plasma lipid levels while a diet with PF as source of lipids changed the animals' lipid profile.

Lipidic profile among rats submitted to total splenectomy isolated or combined with splenic autotransplant.

PURPOSE: To evaluate the alterations on plasmatic lipids levels among rats submitted to total splenectomy isolated or combined with splenic autotransplant receiving standard chow during the postoperative period. METHODS: Thirty Wistar rats were divided into three groups: control (C)-- sham-operated, total splenectomy--isolated (TS) or combined with splenic autotransplantation (SA). Since the postoperative period, all animals received standard rat chow manipulated in accordance to the American Institute of Nutrition Rodents Diets (1993). The plasmatic levels of total cholesterol (TC), triglycerides (TG), high-density lipoprotein (HDL), low-density lipoprotein (LDL), very-low-density lipoprotein (VLDL), and glucose (GLUC) were analyzed before the surgical procedure and after 6 and 12 weeks. RESULTS: All the animals presented significant increase of TG and VLDL levels. In relation to the other parameters there was no difference among the weeks 0 and 12 in the animals of group C. In TS group significant increase was observed in TC and GLUC levels during the experiment. In SA group TC, HDL, and GLUC levels remained unaffected while HDL levels increased. CONCLUSION: Our findings suggest that isolated total splenectomy alters lipids metabolism in rats fed with standard chow and splenic autotransplantation is effective in restoring its control.

Lipidic profile among rats submitted to total splenectomy isolated or combined with splenic autotransplant

PURPOSE: To evaluate the alterations on plasmatic lipids levels among rats submitted to total splenectomy isolated or combined with splenic autotransplant receiving standard chow during the postoperative period. METHODS: Thirty Wistar rats were divided into three groups: control (C) - sham-operated, total splenectomy - isolated (TS) or combined with splenic autotransplantation (SA). Since the postoperative period, all animals received standard rat chow manipulated in accordance to the American Institute of Nutrition Rodents Diets (1993). The plasmatic levels of total cholesterol (TC), triglycerides (TG), high-density lipoprotein (HDL), low-density lipoprotein (LDL), very-low-density lipoprotein (VLDL), and glucose (GLUC) were analyzed before the surgical procedure and after 6 and 12 weeks. RESULTS: All the animals presented significant increase of TG and VLDL levels. In relation to the other parameters there was no difference among the weeks 0 and 12 in the animals of group C. In TS group significant increase was observed in TC and GLUC levels during the experiment. In SA group TC, HDL, and GLUC levels remained unaffected while HDL levels increased. CONCLUSION: Our findings suggest that isolated total splenectomy alters lipids metabolism in rats fed with standard chow and splenic autotransplantation is effective in restoring its control.

OBJETIVO: Avaliar as alterações nos níveis de lipídios plasmáticos em ratos submetidos a esplenectomia total isolada ou combinada com auto-implante esplênico, recebendo dieta padrão no período pós-operatório. MÉTODOS: Trinta ratos Wistar foram distribuídos em três grupos: controle (C) - operação simulada, esplenectomia total isolada (ET) ou combinada com auto-implante esplênico (AE). A partir do período pós-operatório, todos os animais receberam ração padrão, manipulada segundo o American Institute of Nutrition (1993). Os níveis plasmáticos de colesterol total (CT), triglicerídeos (TG), lipoproteína de alta densidade (HDL), lipoproteína de baixa densidade (LDL), lipoproteína de muito baixa densidade (VLDL) e glicose (GLIC) foram analisados antes do procedimento e após 6 e 12 semanas. RESULTADOS: Todos os animais apresentaram aumento de TG e VLDL. Em relação aos demais parâmetros, não ocorreu diferença entre as semanas 0 e 12 nos animais do grupo C. No grupo ET, observou-se aumento de CT e GLIC durante o experimento. No grupo AE, os níveis de CT, HDL e GLIC permaneceram inalterados enquanto os níveis de HDL se elevaram. CONCLUSÃO: Nossos achados sugerem que a esplenectomia total isolada altera o metabolismo lipídico em ratos alimentados com ração padrão e que o auto-implante esplênico é eficaz na restauração do seu controle.

Lipidic profile among rats submitted to total splenectomy isolated or combined with splenic autotransplant / Perfil lipídico em ratos submetidos a esplenectomia total isolada ou combinada com auto-implante esplênico

PURPOSE: To evaluate the alterations on plasmatic lipids levels among rats submitted to total splenectomy isolated or combined with splenic autotransplant receiving standard chow during the postoperative period. METHODS: Thirty Wistar rats were divided into three groups: control (C) - sham-operated, total splenectomy - isolated (TS) or combined with splenic autotransplantation (SA). Since the postoperative period, all animals received standard rat chow manipulated in accordance to the American Institute of Nutrition Rodents Diets (1993). The plasmatic levels of total cholesterol (TC), triglycerides (TG), high-density lipoprotein (HDL), low-density lipoprotein (LDL), very-low-density lipoprotein (VLDL), and glucose (GLUC) were analyzed before the surgical procedure and after 6 and 12 weeks. RESULTS: All the animals presented significant increase of TG and VLDL levels. In relation to the other parameters there was no difference among the weeks 0 and 12 in the animals of group C. In TS group significant increase was observed in TC and GLUC levels during the experiment. In SA group TC, HDL, and GLUC levels remained unaffected while HDL levels increased. CONCLUSION: Our findings suggest that isolated total splenectomy alters lipids metabolism in rats fed with standard chow and splenic autotransplantation is effective in restoring its control.(AU)

OBJETIVO: Avaliar as alterações nos níveis de lipídios plasmáticos em ratos submetidos a esplenectomia total isolada ou combinada com auto-implante esplênico, recebendo dieta padrão no período pós-operatório. MÉTODOS: Trinta ratos Wistar foram distribuídos em três grupos: controle (C) - operação simulada, esplenectomia total isolada (ET) ou combinada com auto-implante esplênico (AE). A partir do período pós-operatório, todos os animais receberam ração padrão, manipulada segundo o American Institute of Nutrition (1993). Os níveis plasmáticos de colesterol total (CT), triglicerídeos (TG), lipoproteína de alta densidade (HDL), lipoproteína de baixa densidade (LDL), lipoproteína de muito baixa densidade (VLDL) e glicose (GLIC) foram analisados antes do procedimento e após 6 e 12 semanas. RESULTADOS: Todos os animais apresentaram aumento de TG e VLDL. Em relação aos demais parâmetros, não ocorreu diferença entre as semanas 0 e 12 nos animais do grupo C. No grupo ET, observou-se aumento de CT e GLIC durante o experimento. No grupo AE, os níveis de CT, HDL e GLIC permaneceram inalterados enquanto os níveis de HDL se elevaram. CONCLUSÃO: Nossos achados sugerem que a esplenectomia total isolada altera o metabolismo lipídico em ratos alimentados com ração padrão e que o auto-implante esplênico é eficaz na restauração do seu controle.(AU)