RESUMEN
A crucial step in early embryogenesis is the establishment of spatial patterns of signaling activity. Tools to perturb morphogen signals with high resolution in space and time can help reveal how embryonic cells decode these signals to make appropriate fate decisions. Here, we present new optogenetic reagents and an experimental pipeline for creaHng designer Nodal signaling patterns in live zebrafish embryos. Nodal receptors were fused to the light-sensitive heterodimerizing pair Cry2/CIB1N, and the Type II receptor was sequestered to the cytosol. The improved optoNodal2 reagents eliminate dark activity and improve response kinetics, without sacrificing dynamic range. We adapted an ultra-widefield microscopy platform for parallel light patterning in up to 36 embryos and demonstrated precise spatial control over Nodal signaling activity and downstream gene expression. Patterned Nodal activation drove precisely controlled internalization of endodermal precursors. Further, we used patterned illumination to generate synthetic signaling patterns in Nodal signaling mutants, rescuing several characteristic developmental defects. This study establishes an experimental toolkit for systematic exploration of Nodal signaling patterns in live embryos.
RESUMEN
The purpose of this study was to investigate the chronic effect of a contrast training program designed to elicit an acute short term enhancement (STE) effect during training. A matched pairs training study design was implemented with a contrast (STE affected) and complex (control) training group completing a seven week training intervention. Twenty subjects participated. The contrast training group completed training based on a preloading protocol that had previously been shown to induce an acute STE effect within the subject population. The control group completed the same volume and type of training in a complex training format. Changes in squat 4RM strength and kinetic and kinematic performance in vertical and horizontal countermovement jump (CMJ) and drop jump (DJ) were measured via a Force Plate. Differences between the experimental and control group in change of mean strength (effect size 0.03 ± 0.33), vertical DJ (effect size: contact time -0.22 ± 0.52; peak force 0.20 ± 0.30; mean force 0.30 ± 0.74) and horizontal DJ (effect size: contact time -0.47 ± 0.73; peak force 0.03 ± 0.36; mean force 0.13 ± 0.56) were not meaningful. However, differences in mean change of vertical and horizontal CMJ measures of force (effect size range 0.40 - 0.46 ± 0.37 - 0.63), vertical CMJ peak velocity (effect size 0.84 ±0.66) and mean velocity (0.62 ± 0.88) were meaningful. These findings demonstrate that eliciting an acute STE response in dynamic training movements through contrast training can produce a chronic improvement in dynamic movements as a training effect.
RESUMEN
This study investigated the relationship between vertical and horizontal measures in bilateral and unilateral countermovement jump, drop jump and squat jump (SJ), and sprinting speed and muscle architecture of both the vastus lateralis and gastrocnemius. Subjects (n = 17) completed a 30-m sprint test, muscle stiffness test; ultrasound measures, and a jump testing session. Measures of horizontal peak and mean force, in both bilateral and unilateral jumps, tended to have greater relationships to sprint speeds (R = 0.132-0.576) than peak and mean force in the vertical plane (R = 0.008-0.504). Vertical velocity variables also showed some large and very large correlations to sprint speed (R = 0.062-0.635). Unilateral measures of velocity tended to have larger correlations to sprint performance than their bilateral counterparts across all jump types and peak and mean velocity in SJ showed large and very large correlations to sprint speed (bilateral R = 0.227-0.635; unilateral 0.393-0.574). Few large correlations were shown between muscle stiffness measures of muscle architecture and kinetic and kinematic variables in either vertical or horizontal jumps. The present findings suggest that sport scientists and strength and conditioning practitioners concerned with the prognostic value of kinetic variables to functional movements such as sprint speed should also use horizontal jumps in addition to vertical jumps in testing and training.
