Sports game intervention aids executive function enhancement in children with autism - An fNIRS study.

Executive dysfunction is a prevalent issue in children diagnosed with autism spectrum disorder (ASD). While the efficacy of physical exercise in enhancing cognitive abilities in these children is well-documented, research exploring the relationship between physical exercise and brain function remains limited. This study aimed to investigate the impact of cognitively stimulating exercise on executive functions (EF) in children with ASD. The study enrolled thirty children with ASD who were randomly allocated into two groups: a sports game learning group (n = 15) and a control group (n = 15). Functional near-infrared spectroscopy was utilized to monitor cerebral function alterations pre- and post- an eight-week intervention program. The study focused on three core components of executive function: working memory, inhibitory control (IC), and cognitive flexibility (CF). Results revealed a significant improvement in the EF in the intervention group. After eight weeks of intervention, neural activity, along with improved EF performance, was enhanced significantly in the prefrontal cortex (PFC). During post-intervention, EF tasks were also significantly activated in the dorsolateral PFC, orbitofrontal cortex, and frontal pole area. Furthermore, an increase in short-distance functional connectivity within the PFC was observed during resting states. These results imply that engagement in sports game training can significantly improve EF information processing, augmenting task-related cortical activations and the efficiency of brain function networks in children with ASD.

Martial arts enhances working memory and attention in school-aged children: A functional near-infrared spectroscopy study.

Interventions can improve working memory and attention in school-aged children, but little is known about how regional changes in brain activity promoted by exercise mediate this cognitive improvement. This study focused on the improved neurocognitive functions and intrinsic regional variation within the brain by comparing school-aged children in a martial arts group with those in free-play and rest groups. With a pretest-posttest design, the d2 attention test and N-back tasks were carried out. Functional near-infrared spectroscopy was performed during the pre- and post-intervention N-back tasks and rest. Following the intervention, the d2 attention in all groups remarkably increased, and the attention level of the martial arts group was substantially higher than those of the other two groups. Free-play and martial arts shortened the 1- and 2-back task reaction time and increased the 2-back accuracy rate (AR), and the martial arts group exhibited a significantly higher AR than the other two groups. In addition, the martial arts group showed higher activations in the right orbitofrontal cortex and right Broca's area (r-BA) regions post-intervention 1-back tasks, whereas a strong correlation was observed between 1-back performance and the related brain region. However, under the 2-back task, although the cognitive control was improved, the martial arts group decreased activation in the left frontopolar area and free play decreased the activation in the r-BA and right somatosensory cortex. Together, our findings showed that martial arts could be more conducive to cognitive improvement than physical exercise that requires no cognitive skills and that performing interventions in the earlier stages of childhood may improve the regulation of neural networks involved in cognitive control.

Molecular mechanisms of exercise intervention in alleviating the symptoms of autism spectrum disorder: Targeting the structural alterations of synapse.

Autism spectrum disorder (ASD) is a complex and heterogeneous neurodevelopmental disorder characterized by stereotyped behaviors, specific interests, and impaired social and communication skills. Synapses are fundamental structures for transmitting information between neurons. It has been reported that synaptic deficits, such as the increased or decreased density of synapses, may contribute to the onset of ASD, which affects the synaptic function and neuronal circuits. Therefore, targeting the recovery of the synaptic normal structure and function may be a promising therapeutic strategy to alleviate ASD symptoms. Exercise intervention has been shown to regulate the structural plasticity of synapses and improve ASD symptoms, but the underlying molecular mechanisms require further exploration. In this review, we highlight the characteristics of synaptic structural alterations in the context of ASD and the beneficial effects of an exercise intervention on improving ASD symptoms. Finally, we explore the possible molecular mechanisms of improving ASD symptoms through exercise intervention from the perspective of regulating synaptic structural plasticity, which contributes to further optimizing the related strategies of exercise intervention promoting ASD rehabilitation in future.

Overview on brain function enhancement of Internet addicts through exercise intervention: Based on reward-execution-decision cycle.

Internet addiction (IA) has become an impulse control disorder included in the category of psychiatric disorders. The IA trend significantly increased after the outbreak of the new crown epidemic. IA damages some brain functions in humans. Emerging evidence suggests that exercise exerts beneficial effects on the brain function and cognitive level damaged by IA. This work reviews the neurobiological mechanisms of IA and describes the brain function impairment by IA from three systems: reward, execution, and decision-making. Furthermore, we sort out the research related to exercise intervention on IA and its effect on improving brain function. The internal and external factors that produce IA must be considered when summarizing movement interventions from a behavioral perspective. We can design exercise prescriptions based on exercise interests and achieve the goal of quitting IA. This work explores the possible mechanisms of exercise to improve IA through systematic analysis. Furthermore, this work provides research directions for the future targeted design of exercise prescriptions.

Effects of acute exercise on craving and cortical hemodynamics under drug-cue exposure in MA-dependent individuals.

BACKGROUND: Methamphetamine (MA) dependent individuals who want to break free of their drug habit or guard against a relapse often find it hard to overcome cravings induced by drug-related cues they are bound to encounter. The purpose of this study was to investigate the effects of acute virtual reality (VR) enhanced physical exercise on cue-induced cravings in MA-dependent individuals. METHODS: Thirty MA-dependent individuals performed a drug-cue reactivity task both before and after a 10 min VR-enhanced competitive cycling exercise. Functional near-infrared spectroscopy (fNIRS) was recorded during the pre- and post-exercise drug-cue reactivity tasks. RESULTS: MA dependent individuals show higher hemodynamic responses in prefrontal cortex (PFC) to drug-related cues than to neutral cues. After acute exercise, hemodynamic responses in PFC, including bilateral dorsolateral prefrontal cortex and orbitofrontal cortex, were attenuated under the same drug-related cues exposure. Acute exercise also affected the functional connectivity between PFC and motor cortex in response to drug-related cues versus neutral cues. CONCLUSIONS: These results suggest that a single session of VR-enhanced competitive cycling exercise facilitates MA-dependent individuals' self-control over their cue-induced cravings by modulating cortical activations and brain functional networks.

Acute VR competitive cycling exercise enhanced cortical activations and brain functional network efficiency in MA-dependent individuals.

BACKGROUND: Methamphetamine (MA) dependence is associated with elevated rates cognitive impairment in MA users. The objective of the present study was to investigate the effects of virtual reality (VR) competitive cycling excise on the neurocognitive functions and on negative affectivity of MA-dependent individuals. METHODS: Thirty MA-dependent individuals performed a colour-word Stroop task and underwent a profile of mood states (POMS) scale assessment both before and after a 10 min VR competitive cycling exercise. Functional near-infrared spectroscopy (fNIRS) were recorded during the pre-and post-exercise Stroop tasks and during rest. RESULTS: After acute exercise, neural activity, along with improved Stroop performance, was enhanced significantly in the dorsolateral prefrontal cortex. Also observed during post-exercise Stroop tasks was a more efficient network architecture in the topological organization of brain networks than during the pre-exercise Stroop tasks. As for resting states before versus after exercisethe, we detected an increased functional connectivity between the prefrontal cortex and the motor cortex after exercise. CONCLUSIONS: These results suggest that an acute bout of VR competitive cycling exercise facilitates executive information processing by enhancing task-related cortical activations and brain functional network efficiency in MA-dependent individuals.

Moderate exercise ameliorates osteoarthritis by reducing lipopolysaccharides from gut microbiota in mice.

Lipopolysaccharides (LPSs) released by gut microbiota are correlated with the pathophysiology of osteoarthritis (OA). Exercise remodels the composition of gut microbiota. The present study investigated the hypothesis that wheel-running exercise prevents knee OA induced by high-fat diet (HFD) via reducing LPS from intestinal microorganisms. Male C57BL/6 J mice were treated with sedentary or wheel-running exercise, standard diet (13.5% kcal) or HFD (60% kcal), berberine or not according to their grouping. Knee OA severity, blood and synovial fluid LPS, cecal microbiota, and TLR4 and MMP-13 expression levels were determined. Our findings reveal that HFD treatment decreased gut microbial diversity. Increase in endotoxin-producing bacteria, decrease in gut barrier-protecting bacteria, high LPS levels in the blood and synovial fluid, high TLR4 and MMP-13 expression levels, and severe cartilage degeneration were observed. By contrast, voluntary wheel running caused high gut microbial diversity. The gut microbiota were reshaped, LPS levels in the blood and synovial fluid and TLR4 and MMP-13 expression levels were low, and cartilage degeneration was ameliorated. Berberine treatment reduced LPS levels in the samples, but decreased the diversity of intestinal flora with similar changes to that caused by HFD. In conclusion, unlike taking drugs, exercising can remodel gut microbial ecosystems, reduce the circulating levels of LPS, and thereby contribute to the relief of chronic inflammation and OA. Our findings showed that moderate exercise is a potential therapeutic approach for preventing and treating obesity-related OA.

Acute kick-boxing exercise alters effective connectivity in the brain of females with methamphetamine dependencies.

OBJECTIVE: Methamphetamine (METH) dependence, especially among women, is a serious global health problem. Kick-boxing exercise can be used to reduce cue-induced craving and develop a healthy lifestyle for female with METH dependencies. This study aimed to assess acute kick-boxing related changes in effective connectivity (EC) in the brain of females with METH dependencies by using functional near-infrared spectroscopy (fNIRS) signals. METHODS: The fNIRS signals were continuously recorded from the left and right prefrontal cortices (LPFC/RPFC) and left and right motor cortices (LMC/RMC) of 30 female subjects with methamphetamine dependencies (METH group) and 30 age-matched controls (control group) during resting and kick-boxing exercise (training) periods. EC was calculated in the frequency range of 0.01-0.08 Hz. RESULTS: In both resting and training state, the EC levels of METH group were significantly lower than the control group (p < 0.05). The EC levels of control group showed more significantly increased connection types than that of the METH group. CONCLUSION: Acute kick-boxing exercise altered EC in the brain of females with METH dependencies. Furthermore, the efficiency of the information flow between different brain regions in the control group was significantly higher than that in the METH group. This study provides a novel and portable assessment technique for METH rehabilitation in females on the basis of fNIRS signals.

Effects of physical training on brain functional connectivity of methamphetamine dependencies as assessed using functional near-infrared spectroscopy.

OBJECTIVE: This study aims to assess the effects of physical training based on the functional near-infrared spectroscopy (fNIRS) and heart rate signals. METHODS: The oxygenated hemoglobin concentration (Delta [HbO2]) signals were recorded from the left prefrontal cortex (LPFC), right prefrontal cortex (RPFC), left motor cortex (LMC) and right motor cortex (RMC) of 23 subjects with methamphetamine (METH) dependencies at resting, spinning training and strength training states. The wavelet phase coherence (WPCO) values were calculated in four frequency intervals: I, 0.6-2; II, 0.145-0.6; III, 0.052-0.145; and IV, 0.021-0.052 Hz. During the spinning training and strength training states, heart rate signals were recorded at 1, 2, 3, 4, 5, 6, 7, 8, 9 and 10 min, respectively. RESULTS: After physical training, the brain regions of LPFC, RPFC and LMC showed different degrees of activation in the subjects with METH dependencies (p <  0.05). The WPCO values between the brain regions significantly altered after spinning training and strength training (p <  0.05) in frequency intervals I, II, III and IV. CONCLUSIONS: The altered WPCO values indicated physical training could affect brain functional connectivity (FC) to a certain extent in the subjects with METH dependencies. These findings provide a method for the assessment of the effects of physical training in FC and will contribute to the development of drug rehabilitation methods in subjects with METH dependencies.

Bulbophyllum reflexipetalum (Orchidaceae, Epidendroideae, Malaxideae), a new species from Xizang, China.

Bulbophyllum reflexipetalum, a new species from Motuo County, Southeast Xizang, China, is described and illustrated here. This new species belongs to Bulbophyllum sect. Umbellata Bentham & J. D. Hooker, and it is morphologically similar to B. umbellatum Lindley, B. guttulatum (J. D. Hooker) N. P. Balakrishnan and B. salweenensis X.H. Jin, but is distinguished from them by having reflexed petals, base of dorsal sepal with 1 dentate on each side, lip with significantly revolute margin, adaxially with dark brown spots or patches and one longitudinal groove.

Marsdenia yarlungzangboensis (Apocynaceae, Asclepiadoideae), a new species from Xizang, China.

Marsdenia yarlungzangboensis (Apocynaceae, Asclepiadoideae), a new species from Motuo County, southeastern Xizang of China, is described and illustrated. It is morphologically similar to M. medogensis, M. tenii and M. yuei, the major differences between the new species and the morphological relatives are outlined and discussed. A diagnostic key to the new species and its closely related species in China is provided.

Using complex II as an emerging therapeutic target for the treatment of muscle lesions.

After patients has been trapped into skeletal muscle injury, hypoxic and dysfunctional mitochondria brings about a crisis in energy supply that severely disrupts the repair of skeletal muscle. This study aims to elucidate injury-induced adaptations in the mitochondria and provide statistics for the role of complex II in instilling cells with energy under hypoxic conditions. Fifty-six male Wistar rats were divided into control, 12 h, 2 d, 5 d, 7 d, 10 d, 15 d, and 30 d postinjury groups. Contusion injury was made via an instrumented drop-mass technique delivering single impact to the posterior surface of the gastrocnemius of one limb of the rats. ROS levels, loss of mitochondrial membrane potential (MMP), activities of marker enzymes (miCK, LDH, and ALP), and activities of complexes I-III were determined. Our findings reveal that the first 2 d postinjury, especially at 12 h, is the period with most severe oxidative stress. After injury, the activities of mitochondrial complexes I-III display different behaviors based on time and various energy production mechanisms. Our results highlight that complex II participates in electron transport in the acute phase of blunt trauma. We proposed that CII could be a therapeutic target in muscle lesions.

Effect of aerobic exercise intervention on DDT degradation and oxidative stress in rats.

Dichlorodiphenyltrichloroethane (DDT) reportedly causes extensively acute or chronic effects to human health. Exercise can generate positive stress. We evaluated the effect of aerobic exercise on DDT degradation and oxidative stress. MAIN METHODS: Male Wistar rats were randomly assigned into control (C), DDT without exercise training (D), and DDT plus exercise training (DE) groups. The rats were treated as follows: DDT exposure to D and DE groups at the first 2 weeks; aerobic exercise treatment only to the DE group from the 1st day until the rats are killed. DDT levels in excrements, muscle, liver, serum, and hearts were analyzed. Superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and malondialdehyde (MDA) levels were determined. Aerobic exercise accelerated the degradation of DDT primarily to DDE due to better oxygen availability and aerobic condition and promoted the degradation of DDT. Cumulative oxidative damage of DDT and exercise led to significant decrease of SOD level. Exercise resulted in consistent increase in SOD activity. Aerobic exercise enhanced activities of CAT and GSH-Px and promoted MDA scavenging. Results suggested that exercise can accelerate adaptive responses to oxidative stress and activate antioxidant enzymes activities. Exercise can also facilitate the reduction of DDT-induced oxidative damage and promoted DDT degradation. This study strongly implicated the positive effect of exercise training on DDT-induced liver oxidative stress.

[The changes of HIF-1α and AMPKα2 expression levels during skeletal muscle blunt injury recovery].

OBJECTIVE: To explore the possible biological mechanisms of skeletal muscle injury recovery by observing the changes of AMP-activated protein kinase α2 (AMPKα2) and hypoxia inducible factor-1α (HIF-1α) expression levels of rats in the skeletal muscle blunt injury recovery process. METHODS: Six of the 48 male Wistar rats was randomly selected as the normal control group. The blunt injury model was set up by injuring the hind legs of remaining 42 rats with heavy objects. Then they were divided into 7 groups (n=6). The changes of AMPKα2 and HIF-1α expression levels were tested in the hind limb triceps surae of the model rats from each of the 7 groups at 12 hours, 2 days, 5 days, 7 days, 10 days, 15 days and 30 days respectively following the injury. RESULTS: The expression levels of AMPKα2 and HIF-1α all increased significantly at 12 hours following the injury and fell close to normal levels at 15 days following the injury. The values of AMPKα2 and HIF-1α expression peaked within 2 days after injury and the expression levels began to decline at 5 days after injury. Except the peak, the changes of the mRNA expressions of the two proteins were basically consistent with those of protein expression at the other time points. CONCLUSIONS: HIF-1α and AMPKα2 might play roles in mediating hypoxia adaptation, muscle cell regeneration, and energy compensation to promote recovery after injury.

4-(2,3-Dimeth-oxy-phen-yl)-1H-pyrrole-3-carbonitrile.

The asymmetric unit of the title compound, C(13)H(12)N(2)O(2), obtained in a search for analogs of the fungicide fludioxonil [systematic name: 4-(2,2-difluoro-1,3-benzodioxol-4-yl)-1H-pyrrole-3-carbonitrile], contains two independent mol-ecules, A and B. The benzene and pyrrole rings are inclined to each other at 38.5 (1) and 29.3 (1)° in mol-ecules A and B, respectively. In the crystal, bifurcated N-H⋯(O,O) hydrogen bonds link A mol-ecules into chains along [001], while B mol-ecules are linked into layers parallel to the bc plane via bifurcated N-H⋯(N,N) hydrogen bonds.