RESUMEN
Borehole strain gauges play a crucial role in geophysical, seismological, and crustal dynamics studies. While existing borehole strain gauges are proficient in measuring horizontal strains within vertical boreholes, their effectiveness in capturing vertical and oblique strains is limited due to technical constraints arising from the cylindrical probe's characteristics. However, the accurate measurement of three-dimensional strain is essential for a comprehensive understanding of crustal tectonics, dynamics, and geophysics, particularly considering the diverse geological structures and force sources within the crustal medium. In this study, we present a novel approach to address this challenge by enhancing an existing horizontal-component borehole strain gauge with a bellows structure and line strain measurement technology to enable vertical and borehole oblique strain measurements. Integrating these enhancements with horizontal strain measurement capabilities enables comprehensive three-dimensional borehole strain measurements within the same hole section. The system was deployed and tested at the Gongxian seismic station in Sichuan Province. Clear observations of solid tides were recorded across horizontal, oblique, and vertical measurement units, with the tidal morphology and amplitude being consistent with the theoretical calculations. The achieved measurement sensitivity of 10-10 meets the requirements for borehole strain measurement, enabling the characterization of three-dimensional strain states within boreholes through association methods.
RESUMEN
This paper presents a high-precision component-type vertical pendulum tiltmeter based on an FPGA (Field Programmable Gate Array) that improves the utility and reliability of geophysical field tilt observation instruments. The system is designed for rapid deployment and offers flexible and efficient adaptability. It comprises a pendulum body, a triangular platform, a locking motor and sealing cover, a ratiometric measurement bridge, a high-speed ADC, and an FPGA embedded system. The pendulum body is a plumb-bob-type single-suspension wire vertical pendulum capable of measuring ground tilt in two orthogonal directions simultaneously. It is installed on a triangular platform, sealed as a whole, and equipped with a locking motor to withstand a free-fall impact of 2 m. The system utilizes a differential capacitance ratio bridge in the measurement circuit, replacing analog circuits with high-speed AD sampling and FPGA digital signal processing technology. This approach reduces hardware expenses and interferences from active devices. The system also features online compilation functionality for flexible measurement parameter settings, high reliability, ease of use, and rapid deployment without the need for professional technical personnel. The proposed tiltmeter holds significant importance for further research in geophysics.
RESUMEN
In this study, an FPGA(Field Programmable Gate Array)-based borehole strain measurement system was designed that makes extensive use of digital signal processing operations to replace analog circuits. Through the formidable operational capability of FPGA, the sampled data were filtered and denoised to improve the signal-to-noise ratios. Then, with the goal of not reducing observational accuracy, the signal amplification circuit was removed, the excitation voltage was reduced, and the dynamic range of the primary adjustments was expanded to 130 dB. The system's online compilation function made it more flexible to changes in measurement parameters, allowing it to adapt to various needs. In addition, the efficiency of the equipment use was enhanced. The actual observational results showed that this study's FPGA-based borehole strain measurement system had a voltage resolution higher than 1 µV. Clear solid tides were successfully recorded in low-frequency bands, and seismic wave strain was accurately recorded in high-frequency bands. The arrival times and seismic phases of the seismic waves S and P were clearly recorded, which met the requirements for geophysical field deformation observations. Therefore, the system proposed in this study is of major significance for future analyses of geophysical and crust deformation observations.
RESUMEN
The Drosophila Boundary Element-Associated Factor of 32 kDa (BEAF) binds in promoter regions of a few thousand mostly housekeeping genes. BEAF is implicated in both chromatin domain boundary activity and promoter function, although molecular mechanisms remain elusive. Here, we show that BEAF physically interacts with the polybromo subunit (Pbro) of PBAP, a SWI/SNF-class chromatin remodeling complex. BEAF also shows genetic interactions with Pbro and other PBAP subunits. We examine the effect of this interaction on gene expression and chromatin structure using precision run-on sequencing and micrococcal nuclease sequencing after RNAi-mediated knockdown in cultured S2 cells. Our results are consistent with the interaction playing a subtle role in gene activation. Fewer than 5% of BEAF-associated genes were significantly affected after BEAF knockdown. Most were downregulated, accompanied by fill-in of the promoter nucleosome-depleted region and a slight upstream shift of the +1 nucleosome. Pbro knockdown caused downregulation of several hundred genes and showed a correlation with BEAF knockdown but a better correlation with promoter-proximal GAGA factor binding. Micrococcal nuclease sequencing supports that BEAF binds near housekeeping gene promoters while Pbro is more important at regulated genes. Yet there is a similar general but slight reduction of promoter-proximal pausing by RNA polymerase II and increase in nucleosome-depleted region nucleosome occupancy after knockdown of either protein. We discuss the possibility of redundant factors keeping BEAF-associated promoters active and masking the role of interactions between BEAF and the Pbro subunit of PBAP in S2 cells. We identify Facilitates Chromatin Transcription (FACT) and Nucleosome Remodeling Factor (NURF) as candidate redundant factors.
Asunto(s)
Proteínas de Drosophila , Elementos Aisladores , Animales , Ensamble y Desensamble de Cromatina , Nucleosomas/genética , Nucleosomas/metabolismo , Nucleasa Microcócica/genética , Nucleasa Microcócica/metabolismo , Proteínas de Unión al ADN/genética , Proteínas de Unión al ADN/metabolismo , Proteínas de Drosophila/metabolismo , Factores de Transcripción/genética , Factores de Transcripción/metabolismo , Drosophila/genética , Drosophila/metabolismo , Cromatina/genética , Cromatina/metabolismoRESUMEN
The spatiotemporal distributions, influential factors, and ecological risks of 15 phthalate acid esters (PAEs) in the sediments of three typical lakes in Inner Mongolia were investigated in this work. PAEs were widespread in all surface sediments ranging from 598.3 to 3873.8 µg/kg dry weight. Average PAE concentrations were highest in the surface sediments of Wuliangsuhai Lake followed by Daihai and Hulun Lakes, which were consistent with population density and intensity of anthropogenic activities. Dibutyl phthalate, di-iso-butyl phthalate (DIBP), and diethyl phthalate (DEP) were the dominant PAEs with similar abundance orders for the three lakes. The cold climate could explain the higher DEP abundance in the sediments of lakes at higher latitudes. The highest Σ PAE and most individual PAE concentrations were located at one of the discharge river estuaries because most PAEs were terrestrial pollutants. Σ PAE concentrations in the core sediments increased with years and fluctuations owing to the increasing PAE usage and input. The second highest PAE concentration peaks were associated with the high gross domestic product growth rates in 2003-2008. Redundancy analysis revealed that butyl benzyl phthalate and diamyl phthalate in the surface sediments of Hulun Lake were principally positively influenced by organic matter (65.6% contribution among geochemical variables), Fe oxides (25.1% contribution among metals), and Mn oxides (23.8% contribution among metals). The DIBP and DEP exhibited high risks to sensitive fish according to a risk quotient assessment. This work presents new insights into PAE status and deposition mechanisms in the lakes within high latitudes or cold climates, and provides important information for future environmental protection and management.