RESUMEN
Aldehyde dehydrogenase (ALDH) is a key enzyme for the catalytic oxidation of acetaldehyde to acetic acid. Genetic polymorphisms of ALDH2 have been associated with a wide range of diseases and cancers. However, little information is found about the association between ALDH2 polymorphisms and lumbar disc herniation (LDH) in Chinese Han population. We investigated the association between single nucleotide polymorphisms (SNPs) in ALDH2 and LDH risk in a case-control study that included 380 LDH cases and 692 healthy controls. Eight SNPs were selected and genotyped using the Sequenom MassARRAY platform. Odds ratios (ORs) and 95% confidence intervals (CIs) were calculated using unconditional logistic regression after adjusting for gender and age. In the allele model analysis, we found the frequency of the "A" allele of rs671 was significantly higher in LDH cases than in controls (OR = 1.414, 95%CI: 1.109-1.803, P = 0.005). In the genetic model analysis, we found the minor allele "A" of rs671 was associated with increased risk of LDH under log-additive model (OR = 1.42, 95%CI: 1.11-1.82, P = 0.0062); and the minor allele "C" of rs7296651 was associated with decreased risk of LDH under over-dominant model (OR = 0.72, 95%CI: 0.53-0.97, P = 0.031). Additionally, the haplotype "GGCTCACG" constructed by rs886205, rs2238152, rs4648328, rs441, rs4646778, rs671, rs11066028, and rs7296651 was associated with increased risk of LDH (OR = 1.45; 95% CI = 1.11-1.90; P = 0.0071). Our data shed new light on the association between genetic polymorphisms of ALDH2 and LDH susceptibility in a Chinese Han population.
Asunto(s)
Aldehído Deshidrogenasa Mitocondrial/genética , Pueblo Asiatico/genética , Etnicidad/genética , Predisposición Genética a la Enfermedad , Desplazamiento del Disco Intervertebral/enzimología , Desplazamiento del Disco Intervertebral/genética , Vértebras Lumbares/patología , Polimorfismo de Nucleótido Simple/genética , Estudios de Casos y Controles , Femenino , Frecuencia de los Genes/genética , Estudios de Asociación Genética , Haplotipos/genética , Humanos , Patrón de Herencia/genética , Desequilibrio de Ligamiento/genética , Masculino , Persona de Mediana Edad , Oportunidad Relativa , Factores de RiesgoRESUMEN
OBJECTIVE: To objectively evaluate the clinically curative effect of sidong wubu method on closed fracture of upper limbs. METHODS: A single-blind randomized controlled trial was used to divide 654 patients with closed fracture of upper limbs at early stage into two groups. 298 patients in the surgical group were treated with open reduction and internal fixation and 356 patients in the treatment group with sidong wubu method for 6 months as a course of treatment. RESULTS: As for short-term curative effect (after 6 courses of treatment), the total effective rate was 97.7% in the treatment group and 92.9% in the control group, and the excellent and good rate was 83.7% and 76.5% respectively. Fracture-healing time, treatment cost, function-recovering time, scores of symptoms and signs obviously declined in both groups with remarkable difference between the two groups. As to long-term curative effect (after follow-up visit for one year to 5 years and 2 months), there was still noticeable difference (chi2= 7.536, P<0.05) in total curative effect and in excellent and good rate between the two groups. CONCLUSION: With low cost, short treatment course, good function and other advantages, sidong wubu method can be first used to treat closed fracture of upper limbs.