Pneumatosis cystoides intestinalis as a rare cause of non-surgical pneumoperitoneum.

Pneumoperitoneum as a finding on imaging examinations is not always a sign of acute abdomen due to gastrointestinal perforation. These findings must be viewed in connection with the clinical condition and personal history of each patient because they may also indicate a non-surgical or spontaneous pneumoperitoneum. This condition is repeatedly described but very often neglected. This paper presents the case report of a patient with non-surgical pneumoperitoneum where, despite proceeding according to the guidelines, no expected intra-abdominal pathology explaining the patient's problems was found.

Military surgery and field surgical care in the Czech Army.

Military surgery is one of the basic branches of military medicine. It is based on the principles of surgery performed in peacetime, but is significantly different when performed under specific conditions. The aim of the article is to describe these differences and present the current state of providing surgical care in the field in the Czech Army. Medical support in the field, or the treatment and evacuation system of medical services of the armies of NATO member countries, is organized on four levels, matched by medical facilities designated as Role 1-4. Surgical care in the field usually takes place at the level of Role 2 and Role 3. While in the conditions of a foreign military mission, surgical treatment aims to achieve a definitive standard, in a typical war conflict the only goal is to save life and limb and prepare casualties for further evacuation. Additionally, triage of the wounded is an important part of health care in the field at individual levels, the importance of which increases especially in the case of mass casualties. In the military medical service of the Army of the Czech Republic, goals are defined as part of the construction and development of capabilities, which should expand and reinforce the current potential of providing surgical care in the field - currently encompassing two field hospitals capable of Role 2 or 3. The ambitious plan to construct additional field medical elements is difficult to achieve in the expected time horizon and, in addition, it also faces a significant shortage of military surgeons due to the planned scope of capacity increase. Despite all the objective difficulties, however, Czech military surgeons have a high credit in the eyes of the NATO allies, gained during the deployment of the Czech field hospital or of the Czech field surgical team within multinational military units.

Surgical management of colorectal injury in war.

The rate of colorectal trauma is 5-10 % in modern war conflicts. The most common causes include gunshots or shrapnel injuries; the contusion-laceration mechanism occurs in sporadic cases in the war zone. Despite modern surgical procedures, however, it is associated with a high rate of morbidity, especially if it is not diagnosed and treated in time. Surgical management is specified by simple scoring schemes - the colon injury scale, rectal injury scale and the Flint grading system. Colonic resection with primary or delayed anastomosis is not associated with a higher risk of complicated healing and is nowadays preferred over the construction of terminal stomas. These are indicated only for cases with severe hemodynamic instability in traumatic-hemorrhagic or septic shock with severe diffuse peritonitis. Trauma to the intraperitoneal segment of the rectum is treated in the same way as trauma to the colon. An extraperitoneal rectal injury without soft tissue devastation can be treated with or without a transanal suture. On the contrary, devastating injuries to the rectum including the pelvic soft tissues should be primarily controlled with a stoma with delayed reconstruction. Presacral drainage or rectal stump lavage are no longer recommended.

Computed tomography scan can be misleading in the lateralisation of aldosterone excess

Summary: Primary aldosteronism (PA) is characterised by aldosterone hypersecretion and represents a common cause of secondary hypertension. During diagnostic evaluation, it is essential to determine the aetiology of PA since the treatment of unilateral and bilateral disease differs significantly. Adrenal vein sampling (AVS) has been implemented as a gold standard test for the diagnosis of PA subtype. However, due to the AVS complexity, costs and limited availability, many patients with PA are being treated based on the computed tomography (CT) findings. In this article, we present two patients with discrepant CT and AVS results, demonstrating that AVS is the only reliable method for localising the source of aldosterone excess. Learning Points: CT is an unreliable method for distinguishing aldosterone-producing adenoma (APA) from bilateral adrenal hyperplasia (BAH). CT can be misleading in defining lateralisation of the aldosterone excess in case of unilateral disease (APA). AVS is the gold standard test for defining the PA subtype.

[Current status in the treatment of rectal cancer in the Czech Republic regarding the rate of complete pathological response after neoadjuvant therapy--PATOD C20 study 2011-2012]. / Soucasný stav strategie lécby karcinomu rekta v Ceské republice s ohledem na výskyt kompletní patologické odpovedi pri neoadjuvantní lécbe--studie PATOD C20 2011-2012.

INTRODUCTION: Deciding on the strategy in rectal cancer´s treatment requires a complex and multidisciplinary approach. The primary rectal resection is indicated in early stages, while locally advanced tumors should be pretreated by one of the modes of neoadjuvant (chemo) radiotherapy. The main goal of this study was to explore the therapeutic strategy in patients with rectal cancer in the Czech Republic. The second aim was to determine the incidence of the pathological complete response after neoadjuvant therapy. METHODS: This is a retrospective multicenter clinical study, which includes data from all patients with rectal cancer who were treated at participating centers in the period from 01/01/2011 to 31/12/2012. The required data has been passed into the online registry PATOD C20.Three issues have been set up: 1. Characteristics of the center and cooperation with the oncological department; 2. Characteristics of the treatment of patients with rectal cancer; and 3. Detailed analysis of the group of patients with complete pathological response. The analysis was performed with regard to the nature of individual departments, i.e. departments of surgery in university hospitals with complex oncological centres, departments of surgery within complex oncologic centers, and departments of surgery outside complex oncologic centers. RESULTS: In total, 21 departments of surgery in the Czech Republic provided data about 1860 patients with rectal cancer for the study. The treatment strategy for rectal cancer was determined at multidisciplinary seminars at 19 centers (90.5%). Statistically significant differences between the centers were found in the indication for neoadjuvant treatment (p<0.001), rectal resection with anastomosis (p=0.048), and resection without anastomosis (p=0.022). Complete pathological response was found in 61 (8.7%) patients. Positivity of mesorectal lymph nodes (ypN+) was found in the case of ypT0 stage in 7 (9.7%) patients. CONCLUSION: PATOD study showed that therapy of rectal cancer is highly heterogeneous in the Czech Republic. Despite the best conditions provided, university hospitals and large departments within complex oncologic centers do not fully utilize this benefit.

Small carcinomas involving less than one-quarter of the rectal circumference: local excision is still associated with a high risk of nodal positivity.

AIM: A study was carried out to determine the relationship between mesorectal lymph nodal involvement and T stage in a group of patients with a rectal cancer involving less than one-quarter of the rectal circumference, such as might be selected for local excision. METHOD: The data of patients having rectal resection between 2010 and 2014 were prospectively entered in a rectal carcinoma registry. A model for describing tumours involving less than one quadrant of the rectal circumference was created to facilitate the evaluation process. RESULTS: In all, 304 patients were included in the study. In 68 (22.4%) a small tumour (< 1 quadrant involved) was found. Of these, 26.5% had positive mesorectal lymph nodes (N+). In lesions of Stage ypT0 cancer 12.5% patients were node positive, in Stage Tis and T1 tumours there was no case of node positivity, but in Stage T2 and Stage T3 cancers the incidence of node positivity was 27.5% and 64%. CONCLUSION: The study demonstrated that, even for small tumours involving only one rectal quadrant, the risk of lymph nodal involvement was about 25%. Had the patients undergone local excision the treatment would have been incomplete.

[Predicting pN positivity in T3 rectal cancer]. / Moznosti predikce pN pozitivity u T3 nádoru rekta.

INTRODUCTION: Stage pN+ is a factor which determines the strategy for treatment of T3 rectal cancer. The sensitivity of preoperative imaging examinations revealing N+ is not entirely satisfactory. Risk factors that are associated with pT3pN+ stage and that are detectable by preoperative examination have not been reliably identified. The aim of the study is to analyze the predictive factors determining lymph node involvement in T3 rectal cancer. MATERIAL AND METHODS: Patients with rectal resection for (y)pT3 rectal cancer were analysed. All of the surgical interventions were performed at the Department of Surgery, University Hospital in Hradec Kralove, from 1 January 2011 to 28 February 2014. Data were prospectively collected and saved in the Rectal Cancer Oncologic Register. The parameters studied were age, gender, tumour localisation and its circumferential topography, preoperative chemoradiotherapy, absolute number of harvested lymph nodes and the number of positive lymph nodes in each specimen, tumour grading, presence of lymphovascular invasion and perineural invasion, and the depth of tumour penetration. RESULTS: After selection, 89 patients with T3 rectal cancer were included into the study. Resection for cancer of the upper rectum was performed in 22 (24.7%) patients, middle rectum in 37 (41.6 %) and lower rectum in 30 (33.7%) patients. 38 (42.7%) patients underwent primary operation, 41 (46.1%) patients received neoadjuvant chemoradiotherapy, and radiation therapy was administered to only 10 (11.2%) patients. Stage pN+ was found in 51 (57.3%) patients. Statistical analysis was used to identify the risk factors for pN+: lymphovascular invasion (p0.001), angioinvasion (p=0.030) and perineural invasion (p=0.010). On the border of statistical significance for pN+, low grading of the tumour (p=0.084) was found. The depth of penetration of the tumour into the mesorectum was not statistically significant (p=0.230). CONCLUSION: Our study has shown that pN positivity is associated with lymphovascular invasion, perineural invasion and low grading of the tumour. Accurate identification of these factors before treatment, however, remains very difficult.

[Complicated mesenteric ischaemia]. / Komplikovaná mezenteriální ischemie.

Acute mesenteric ischaemia is a serious condition associated with high morbidity and mortality. Atherosclerosis is one of the most frequent etiologic factors. This case report presents a patient suffering from acute mesenteric ischaemia complicated by acute lower limb ischaemia and post-ischaemic stenosis of the ileum. Such a patient requires a multidisciplinary, comprehensive, and, in some cases, phased approach to solve complications brought about especially by generalized atherosclerosis.

[Pilonidal sinus - possibilities surgical treatment]. / Pilonidální sinus - moznosti operacního resení

INTRODUCTION: Pilonidal sinus is a skin disease affecting the sacrococcygeal region caused by chronic irritation of the skin by ingrowing hairs that get pushed into skin sinuses and follicles. The acute form of the disease which is presented by subcutaneous abscesses very often develops into chronic suppurative subcutaneous fistula. The incidence of pilonidal sinus is approximately 26/100,000 people. Radical surgical excision using skin flaps is essential for successful treatment. MATERIALS AND METHODS: The results of surgical treatment were analysed retrospectively. All surgical procedures evaluated were performed at the Department of Surgery, University Hospital in Hradec Kralove, from January 2010 to June 2014. The parameters analysed were gender, age, method used to close the defect, length of hospital stay, overall morbidity including pilonidal sinuses recurrence. RESULTS: 141 patients undergoing radical excision for pilonidal sinus were analysed. Primary suture was used in 101 (71.6%) patients. Limberg flap reconstruction was performed in 16 (11.3%) patients and Karydakis procedure was used in 24 (17.0%) patients. The length of hospital stay was 4.2 days. 19 operations were performed for recurrent pilonidal sinus. Norecurrence occurred after Karydakis procedure, there were 3 cases of recurrence after Limberg flap reconstruction, and 8 patients experienced recurrence after primary suture. The overall incidence of recurrence was 7.8%. Surgical site complications were recorded mostly after primary suture (37 patients, 26.2%). Conversely, surgical site complications appeared in the Limberg flap group in 2 patients (12.5%) and in theKarydakis procedure group in 1 patient (4.2%). CONCLUSION: Limberg flap reconstruction and Karydakis procedure lead to better results in pilonidal sinus surgery, especially as regards lower recurrence rates and overall morbidity. Both of these procedures are relatively easy to perform and technically undemanding.

[Rectal cancer within 10 cm. Comparison of the radicality of laparoscopic and open surgical techniques with regard to the circumferential resection margin and the completeness of mesorectal excision]. / Karcinom rekta do 10 cm Srovnání radikality laparoskopické a otevrené operacní techniky s ohledem na cirkumferentní resekcní okraj a na kompletnost mezorektální excize.

INTRODUCTION: The issue of achieving radical circumferential margin in laparoscopic rectal surgery has not yet been satisfactorily clarified. In this paper we have focused on circumferential margin assessment and the quality of the mesorectal excision, comparing laparoscopic and open resection for cancer of the middle and lower rectum. MATERIAL AND METHODS: The results of surgical procedures for middle and low rectal cancer were analysed. All the interventions were performed at the Department of Surgery, Teaching Hospital in Hradec Kralove, during the period from January 2011 to December 2012. The data were prospectively collected and entered in the Rectal Cancer Registry. Age, gender, BMI, tumour localisation and topography, the clinical stage, preoperative chemoradiotherapy and response to it, the type of surgery, distal and circumferential margin characteristics, mesorectal excision quality, pT and pN were compared for laparoscopic and open surgery. RESULTS: A total of 161 patients were operated on for rectal cancer during the abovementioned period. 94 patients were included in the trial following selection. Laparoscopy was used in 40 patients and open surgery in 54 patients. Laparoscopic approach was performed in 33 (82.5%) low anterior resections (including four intersphincteric resections), 6 (15%) abdominoperineal amputations and 1 (2.5%) Hartmanns procedure. Open surgery was used for 26 (48.1%) low anterior resections, 21 (38.9%) APR and 7 (13%) Hartmanns procedures. Complete mesorectal excision was achieved in 45% of the laparoscopic resections vs. 46.3% of open resections. Nearly complete excision was performed in 22.5% and 11.1%, respectively. Finally, incomplete excision was described in 30% vs. 38.9%. No available data for TME was detected in three patients. The differences in TME were not statistically significant. Positive circumferential margin was found in 5 (12.5%) patients in the laparoscopy group; on the contrary, in the group undergoing open surgery, pCRO+ was found in 15 (27.8%) patients. Here, too, the results were not statistically significant. When patients without preoperative chemoradiotherapy were excluded, the relationship between ypCRM in the laparoscopy and open surgery group was on the border of statistical significance (Fischer=0.0556). CONCLUSION: As has been shown in our trial, the outcomes of laparoscopic and open approach in rectal cancer treatment are very similar. Particularly, mesorectal excision quality and negative CRM results have proven that the laparoscopic technique is safe and comparable to open surgery in rectal cancer treatment.

Clinical features and therapeutic outcomes of patients with acromegaly: single-center experience.

The aim of this study was to review the outcome of acromegaly treatment, as well as co-morbidity and mortality in a series of patients with acromegaly attending a single center in the last 10 yr. In that period, 53 patients were treated for acromegaly. Transsphenoidal operation was applied as the first-line therapy in 94.3% of patients and it led to disease remission in 59.2% of them. The remission criteria included a nadir GH<1 µg/l after glucose load, and normal age-related IGF-I levels. The remission rate after transsphenoidal surgery was significantly higher in the group of patients with microadenoma (76.9%), than in the group of patients with macroadenoma (52.8%). Patients with invasive tumors had remission rate of 16.7% after transsphenoidal surgery. There were no perioperative deaths. As the second-line treatment somatostatin analogues, radiotherapy, and dopaminergic agonists were used. Hypertension and diabetes were the most frequent co-morbidities in the group of patients. After successful treatment, 30% of patients with diabetes or impaired glucose tolerance had significant improvement of glycemic control. Transsphenoidal surgery is the appropriate firstline therapy in patients with somatotropinoma. Medical and radio-therapy should be reserved as the second-line therapy after surgery failure.

[Numbers of lymph nodes in large intestinal resections for colorectal carcinoma]. / Pocet uzlin u resekcí tlustého streva pro kolorektální karcinom.

INTRODUCTION: Precise evaluation of lymph nodes in the surgical specimen is crucial for the staging and subsequent decision about the adjuvant therapy in colorectal cancer. Prognosis of the patients can be assessed only in cases when at least 12 lymph nodes in the surgical specimen are examined. AIM OF THE WORK: To evaluate the radicalism of resections for colorectal carcinoma after introducing laparoscopic approach. METHODS: We compared all resections for primary colorectal cancer and rectal cancer (C 18-C20) performed in the Department of Surgery in University Hospital Hradec Králové in the years 2005 and 2008 and we evaluated numbers of examined lymph nodes in the surgical specimens. The patients with recurrent tumours and the patients with complete pathological response (negative histology) after neoadjuvant therapy were excluded from the study. RESULTS: 117 patients were included in the study in 2005, 2 of them were operated laparoscopically. 155 patients (more by 32.5%) were included in the study in 2008, 53 of them (34.2%) were operated laparoscopically. In tumours of the right part of the colon (C180-C184) treated by right hemicolectomy: on an average 7.9 (+/- 5.3) lymph nodes were examined in the specimens in 2005, and 15.3 (+/- 7.0) lymph nodes in 2008. In tumours of the left part of the colon (C185-C186) treated by left hemicolectomy: 6.5 (+/- 5.1) lymph nodes were examined in 2005, and 19.6 (+/- 15.6) in 2008. In tumours of the sigmoid colon (C187) 9.1 (+/- 6.9) lymph nodes were examined in 2005,and 15.4 (+/- 7.9) in 2008. In tumours of the rectosigmoid junction (C19) 8.0 (+/- 6.9) lymph nodes were examined in 2005, and 17.8 (+/- 11.2) in 2008. In rectal cancer (C20) 5.2 (+/- 4.5) lymph nodes were examined in 2005, and 13.6 (+/- 12.5) in 2008. There is a significant difference in a number of examined lymph nodes in patients without neodadjuvant treatment compared to those with neoadjuvant chemoradiotherapy and neoadjuvant radiotherapy. In 2005, in an average 3.7 (+/- 3.3) lymph nodes were removed in rectal resections after neoadjuvant chemoradiotherapy, in 2008 in an average 7.6 (+/- 6.1) lymph nodes were removed. In 2005, in an average 5.1 (+/- 3.7) lymph nodes in rectal resections after neoadjuvant radiotherapy were removed, in 2008 6.3 (+/- 4.3) lymph nodes were removed. In 2005, in an average 7.0 (+/- 5.5) lymph nodes in rectal resections without neoadjuvant therapy were removed, in 2008 20.9 (+/- 14.1) lymph nodes were removed. Laparoscopic resections were comparable with open resections regarding the number of examined lymph nodes in our group of patients. CONCLUSION: Introducing the laparoscopic approach to resections of colorectal carcinomas did not decrease radicalism of the operation as to the number of removed lymph nodes.

[Transgastric and transrectal hybrid NOTES cholecystectomy--experimental study]. / Hybridní NOTES cholecystektomie transgastrickým a transrektálním prístupem v experimentu.

INTRODUCTION: Natural Orifice Transluminal Endoscopic Surgery (NOTES) is a dynamically developing branch of miniinvasive surgery, whose aim is a minimazing abdominal wall injury. Many experimental studies describe the wide spectrum of the NOTES techniques in the animal model. The NOTES experiment begun in the porcine model for acquiring experience at the Surgical Departement in the University Hospital in Hradec Králové. MATERIAL AND METHODS: Hybrid NOTES cholecystectomy with transgastric and transrectal approaches in the porcine model were performed there. Eight animals were operated and were observed for 7 to 14 days after operations. A post-operative morbidity and mortality was found as well as a post-mortal examination for intraperitoneal complications. RESULTS: Three transgastric and five transrectal hybrid cholecystectomies were performed. One animal died immediately after operation due to an anaesthesiological complication. There were two transrectal operations that converted to open surgery due to massive rectum wall injury. Seven surviving animals were without complications in the post-operative period. No post-operative complication in post-mortal exploration were found, gastrotomy and colotomy were healed primarily, there were no signs for peritonitis. CONCLUSION: The experimental study showed the posibility of futher continuing development of the hybrid NOTES technique in the porcine model, also its safety, pitfalls and complications were presented.

[Peroperative diagnostics of acute bowel ischemia using ultraviolet light and fluorescein dye]. / Peroperacní diagnostika ischemie streva za pouzití fluoresceinu a ultrafialového svetla.

Acute bowel ischemia continues to have a high mortality rate. The main factor related to this poor outcome is considered to be the delay in diagnosis. The ability to detect ischemia early and to assess the extent of bowel involvement, are the most important aspects of successful treatment. The combination of ultraviolet (UV) light and fluorescein dye would be considered a simple, reliable and technically easy procedure for diagnosis of intestinal ischemia. The method can be used both for laparotomy when the source of UV light is a Wood's lamp as well for laparoscopy when the optical filters are placed to the light source of laparoscopic set to produce UV light. Present clinical experience shows that the method is precise, objective and accessible and that it gives a greater amount of independence to the surgeon allowing him to make the diagnosis of intestinal ischemia without having to rely on the assistance of other specialists.

[Sacral nerve stimulation in the treatment of anal incontinence]. / Stimulace sakrálního nervu v lécbe neurogenní anální inkontinence.

Anal incontinence is defined as the loss of control of defecation. An estimated prevalence is about 20%, it increases with age and women are affected more frequently. Sacral nerve stimulation is a new treatment method with success rate nearly of 80%. It consists of two phases, temporary percutaneous nerve evaluation and permanent stimulation. The best results may be achieved in patients with anatomically intact sphincters, although indication criteria are still not uniform. Mechanism of action remains also unclear. Authors summarise aetiology of anal incontinence, describe the method of sacral nerve stimulation, indications and bring present published results of this method. The aim of this paper is to inform about the new treatment option which has not been yet used in the Czech Republic.

Decreased peak bone mass is associated with a 3-bp deletion/insertion of the CYP19 intron 4 polymorphism: preliminary data from the GOOS study.

Finding that estrogen plays an important role in bone homeostasis in men prompted research on relationship of polymorphism at the CYP19 gene and the bone mass. Therefore, influence of 3-bp deletion/insertion polymorphism of CYP19 (TTTA)7 allele on the peak bone mass attainment in males was studied. Fifty-eight unrelated male participants, aged 21-35, were selected depending on the presence of (TTTA)7 (no.=19) or (TTTA)7-3 (no.=39) alleles from the initial cohort of 92 young males. Heterozygotes (TTTA)7/(TTTA)7-3 (no.=13) were not included in the analysis. Serum levels of estradiol, free testosterone, 25-hydroxyvitamin D, bone alkaline phosphatase, osteocalcin, and beta-crosslaps were measured. Bone mass was measured by DXA at the hip and at the spine. (TTTA)7-3 allele was associated with significantly lower femoral neck bone mineral density (BMD) (p=0.02). Logistic regression model indicated strong association of (TTTA)7-3 allele with low BMD in the range of osteopenia/osteoporosis (p=0.014, odds ratio 12.36, confidence intervals 1.65-92.46). In the present study association of 3-bp deletion polymorphism of the (TTTA)7 allele with decreased peak bone mass in males is reported for the first time. However, further studies are necessary to elucidate the functional relevance of this polymorphism.

Multiple stress fractures in a young female runner.

The effect of exercise on female's bone metabolism has received much attention in recent years. We report on unusual case of a female runner with low body mass and amenorrhea, who suffered 4 stress fractures. Three of the stress fractures occurred during her sports career, and the fourth occurred 7 years after the cessation of sports activities. It seems that exercise-induced amenorrhea together with food restriction in the young age may cause long-term consequences on bone metabolism.

Influence of high intensity training on menstrual cycle disorders in athletes.

AIM: To estimate the influence of intensive training on menstrual cycles in female athletes. METHOD: The questionnaire was used to determine the time of menarche, and the prevalence of primary and secondary amenorrhea and dysmenorrhea in 72 active female athletes from Zagreb (10 volleyball players, 18 basketball players, 10 ballet dancers, and 34 runners) aged between 15 and 21. The control group comprised 96 girls of the same age not engaged in any sports activity. RESULTS: The prevalence of secondary amenorrhea was three times higher in athletes than in the control group (p=0.037). The prevalence of primary amenorrhea was substantially higher in athletes than in the control group (6/72 vs. 0/96, p=0.014), whereas the prevalence of dysmenorrhea was twofold lower in athletes than in the control group (p<0.001). The highest prevalence of secondary amenorrhea was recorded in runners (14/31), particularly long-distance runners (11/17), whereas there was only one case of secondary amenorrhea among basketball players. Menarche was significantly delayed in the athletes who started physical activities before the onset of menstruation (13.8+1.4 vs. 12.6+1.0 years, p<0.001). CONCLUSION: High-intensity training before menarche postpones its onset. Type of training may be related to a significantly higher prevalence of secondary amenorrhea in runners than in basketball players.