RESUMEN
We have developed a fluorescence-based fiber-optical biosensor, which can selectively detect different antibodies in serial at preselected positions inside a single piece of fiber. The fiber is a microstructured polymer optical fiber fabricated from TOPAS cyclic olefin copolymer, which allows for UV activation of localized sensor layers inside the holes of the fiber. Serial fluorescence-based selective sensing of Cy3-labelled α-streptavidin and Cy5-labelled α-CRP antibodies is demonstrated.
Asunto(s)
Anticuerpos/aislamiento & purificación , Técnicas Biosensibles/métodos , Fibras Ópticas , Carbocianinas/química , Fluorescencia , Humanos , Polímeros/química , Estreptavidina/químicaRESUMEN
We present what is believed to be the first microstructured polymer optical fiber (mPOF) fabricated from Topas cyclic olefin copolymer, which has attractive material and biochemical properties. This polymer allows for a novel type of fiber-optic biosensor, where localized sensor layers may be activated on the inner side of the air holes in a predetermined section of the mPOF. The concept is demonstrated using a fluorescence-based method for selective detection of fluorophore-labeled antibodies.
Asunto(s)
Técnicas Biosensibles , Óptica y Fotónica , Polímeros/química , Alquenos/química , Materiales Biocompatibles/química , Proteína C-Reactiva/química , Carbocianinas/química , Tecnología de Fibra Óptica , Microscopía Fluorescente/instrumentación , Microscopía Fluorescente/métodos , Fibras Ópticas , Polimetil Metacrilato/química , Estreptavidina/química , Rayos UltravioletaRESUMEN
We demonstrate selective detection of fluorophore labeled antibodies from minute samples probed by a sensor layer of complementary biomolecules immobilized inside the air holes of microstructured Polymer Optical Fiber (mPOF). The fiber core is defined by a ring of 6 air holes and a simple procedure was applied to selectively capture either alpha-streptavidin or alpha-CRP antibodies inside these air holes. A sensitive and easy-to-use fluorescence method was used for the optical detection. Our results show that mPOF based biosensors can provide reliable and selective antibody detection in ultra small sample volumes.