Croton macrostachyus Leaf Extract-Mediated Green Synthesis of ZnO Nanoparticles and ZnO/CuO Nanocomposites for the Enhanced Photodegradation of Methylene Blue Dye with the COMSOL Simulation Model.

The photodegradation of organic pollutants using metal oxide-based catalysts has drawn great attention as an effective method for wastewater treatment. In this study, zinc oxide nanoparticles (ZnO NPs) and zinc oxide/copper oxide nanocomposites (ZnO/CuO NCs) were fabricated using the leaf extract of Croton macrostachyus as a nontoxic, natural reducing and stabilizing agent. The synthesized samples were characterized by employing X-ray diffraction, microscopic, spectroscopic, and electrochemical methods. The results confirmed the successful synthesis of ZnO NPs and ZnO/CuO NCs with well-defined crystalline structures and morphologies. The prepared samples were tested for the photodegradation of methylene blue (MB) dye under visible light irradiation. Compared to ZnO NPs, ZnO/CuO NCs showed greatly improved photocatalytic performances, particularly with the sample prepared with the 20 mol % Cu precursor (97.02%). The enhancement could be related to the formed p-n heterojunction, which can suppress the recombination of charge carriers and extend the photoresponsive range. A theoretical study of the photocatalytic activity of ZnO/CuO NCs against MB dye degradation was also conducted by using COMSOL Multiphysics software. The results of the simulation are in reasonable agreement with those of the experiment. This study contributes to the development of sustainable and effective photocatalytic materials that are suitable for application in environmental remediation, particularly in the treatment of wastewater.

Impact of Alternative Fungicides on Grape Downy Mildew Control and Vine Growth and Development.

Grapevine downy mildew (GDM) is one of the most serious diseases of grapevines. With limitations in the use of copper-based products imposed for organic agriculture by the European Union, research for alternatives is encouraged. The aim of this research was to follow a 2-year trial to evaluate the control of GDM using some alternative compounds, and to determine their effects on shoot growth, plant photosynthesis, and grape quality and quantity. Under low disease pressure, Bordeaux mixture, copper hydroxide, laminarin combined with low copper, and 0.5 and 0.8% chitosan had the lowest GDM incidence, reduced on leaves by 96, 95, 75, 56, and 81%, respectively, compared with the untreated control in the last survey. With high disease pressure, Bordeaux mixture, laminarin combined with Saccharomyces extracts, and 0.5 and 0.8% chitosan had the lowest GDM incidence, reduced on grape by 86, 37, 66, and 75%, respectively, compared with the untreated control in the survey of mid-July. Chitosan at 0.8% lowered net photosynthesis, due to reduced stomatal conductance, leaf area, and dry weight, with no negative effects observed on the quantity of the grape berries and the quality parameters of their juice. Among the alternatives to copper, chitosan provided the best GDM protection and reduced the vigor of the vegetation, inducing physiological changes without negative effects on grape production.

Effects of Bois noir on carbon assimilation, transpiration, stomatal conductance of leaves and yield of grapevine (Vitis vinifera) cv. Chardonnay.

Bois noir (BN) is one of the main phytoplasma diseases of grapevine (Vitis vinifera). It is widespread, and can cause severe losses in European vineyards. The infective agent colonizes phloem elements and induces visible symptoms of leaf yellowing or reddening after a relatively long incubation period. As the most sensitive cultivars to BN, Chardonnay plants were grouped as healthy or symptomatic in spring, based on the records from the previous year. Leaf gas exchange and chlorophyll a fluorescence were measured weekly from July to September in healthy plants, and in symptomatic and asymptomatic leaves from symptomatic plants. The midday relative water content (mRWC) was measured once per month. The detection of phytoplasma DNA by nested-polymerase chain reaction revealed BN infection in symptomatic leaf samples at the end of September. A significant decrease in pigment content and maximum quantum efficiency of photosystem II (Fv/Fm) of these symptomatic leaves was detected from July to September, although in the asymptomatic leaves of the symptomatic plants the net photosynthesis (Pn) decrease was not significant. In the leaves from the healthy plants, Pn and transpiration were relatively stable. Of note, in July, an initially healthy plant showed a strong Pn reduction that was followed by visible leaf yellowing symptoms only in August. The phytoplasma infection also stimulated significant reductions in mRWC of the symptomatic leaves, with a final large decrease in yield.