Influence of Ascophyllum nodosum algae extract on finishing growth performance and nutrient digestibility of buffalo calves in warm climates.

The present experiment was conducted to investigate the effect of using an extract of Ascophyllum nodosum algae (Tasco®, AN) on feed digestibility and finishing performance of buffalo calves. Twenty-four buffalo calves (263.4 ± 7.1 kg, 6 ± 1.2-month-old) were allocated to three treatments as a completely randomized design. The experimental treatments included (1) basal diet without supplement (control diet, AN0), (2) basal diet supplemented with 1% (AN1), and (3) basal diet supplemented with 2% (AN2). The gas production parameters and digestibility of feed as well as growth performance of buffalo calves were investigated. The gas production parameters of the experimental diets were not affected by treatments. The average feed intake on the whole period of experiment in the control and AN1 was maximum and minimum, respectively. The AN1 buffalo calves had the largest final weight, total weight gain throughout the entire period (day 0 to 75) and from day 30 to 60 of the experiment (P < 0.05). In comparison with the control (i.e., AN0), overall, the feed conversion ratio in AN1 and AN2 calves improved by about 21.4 and 16.3%, with the AN1 indicating the most suitable feed conversion ratio (P < 0.05). In comparison with the control, supplementation of diets with algae extract linearly improved (P < 0.05) the digestibility of nutrients. Data suggested that inclusion of algae extract had the best beneficial effects on the performance and digestibility of nutrients in finishing buffaloes. Therefore, it may be beneficial to use algae extract especially in warm or hot regions of the world such as the Khuzestan province, though more experiment is required to ensure its effect on heat stress.

Comparison digestibility and protozoa population of Khuzestan water buffalo and Holstein cow.

The major aim of this study was to compare the morphology and activity of rumen protozoa of Khuzestan water buffalo and Holstein cow using in vitro digestibility and gas production parameters of steam treated sugarcane pith. Rumen fluid obtained from two buffalo and cow steers fed the same diet, 30:70 concentrate: forage. To separate rumen protozoa, antibiotic solution and fungicides were added to rumen fluid. The results of present experiment indicated that the neutral detergent fiber (NDF; 7.8 vs. 1.69%) and acid detergent fiber (ADF; 6.24 vs. 3.24%) digestibility of steam treated sugarcane pith by rumen protozoal population of Khuzestan buffalo was higher than those of cow (p < 0.05). Also, digestibility of dry matter, NDF and ADF by whole buffalo micro-organisms was more than those in cow (p < 0.05). The results indicated that the potential of gas production of sugarcane pith by rumen protozoa in water buffalo was more than that of cow (p < 0.05). Total rumen ciliate protozoa numbers in water buffalo were significantly higher than those of cow (3.68 × 10(5) vs. 2.18 × 10(5) mL(-1) of rumen content) (p < 0.05). The number of Diplodinium in buffalo was more than that of cow (41.27 vs. 35.7% of total rumen protozoa, respectively). Percentage of Entodinium, Epidinium, Ophryoscolex and Isotricha in cow was more than those of buffalo. Therefore, in the same diet, protozoa and total rumen micro-organisms of Khuzestan water buffalo have higher digestion activity compared to Holstein cow.