RESUMEN
Cytochrome P4501A1 (CYP1A1) is involved in the metabolism of several genotoxic/carcinogenic environmental xenobiotics including polycyclic aromatic hydrocarbons (PAHs) like benzo[a]pyrene. Several authors had proposed CYP1A inhibition as a plausible strategy for cancer chemoprevention. Using ethoxyresorufin O-deethylase activity (EROD), we tested the inhibitory properties of nine flavonoids: quercetin, miricetin, luteolin, fisetin, morin, kaempferol, 5-hydroxyflavone (5-HF), 3-hydroxyflavone (3-HF), and flavone (F) against human recombinant CYP1A1. The last three compounds exerted the highest inhibitory effect with IC50 values of 0.07, 0.10 and 0.08⯵M respectively; the more hydroxyl-groups were present, the lower the potency of inhibition was. Biochemical characterization leads to the conclusion that flavone and its hydroxy derivatives are mixed-type inhibitors. In silico studies have shown that, Phe224 and other aromatic residues in the human CYP1A1 active site play an important role in flavonoid-CYP interaction, through a π/π stacking between the aminoacid and the flavonoid C-ring. Outside the active site, the three flavonoids bind preferentially between A and K helices of the enzyme. Results from the Ames test using human S9 fraction revealed that none of the three compounds was mutagenic. We can consider 5-HF, 3-HF, and F as potential chemopreventive agents against genotoxic damage caused by metabolites resulting from CYP1A1 activity.
Asunto(s)
Antineoplásicos Fitogénicos/farmacología , Citocromo P-450 CYP1A1/antagonistas & inhibidores , Flavonoides/farmacología , Aminoácidos/metabolismo , Antimutagênicos/farmacología , Simulación por Computador , Flavonas/química , Flavonas/farmacología , Humanos , Modelos Moleculares , Simulación del Acoplamiento Molecular , Pruebas de Mutagenicidad , Mutágenos/farmacología , Proteínas Recombinantes , Relación Estructura-ActividadRESUMEN
Mangifera indica stem bark extract (MSBE) is a Cuban natural product which has shown strong antioxidant properties. In this work, the antimutagenic effect of MSBE was tested against 10 well-known mutagens/carcinogens in the Ames test in the absence or presence of metabolic fraction (S9). The chemical mutagens tested included: cyclophosphamide, mitomycin C, bleomycin, cisplatin, dimethylnitrosamine (DMNA), benzo[a]pyrene (BP), 2-acetylaminofluorene (2-AAF), sodium azide, 1-nitropyrene (1-NP) and picrolonic acid. Protective effects of the extract were also evaluated by comparing the efficiency of S9 fraction obtained from rats treated during 28 days with oral doses of MSBE (50-500 mg/kg) with that obtained from rats treated with vehicle (control) to activate bleomycin and cyclophosphamide in the Ames test. MSBE concentrations between 50 and 500 µg/plate significantly reduced the mutagenicity mediated by all the chemicals tested with the exception of sodium azide. Higher mutagenicity was found when bleomycin and cyclophosphamide (CP) were activated by control S9 than by MSBE S9. In addition, inhibition of CYP1A1 microsomal activity was observed in the presence of MSBE (10-20 µg/ml). We can conclude that besides its potent antioxidant activity previously reported, MSBE may also exert a chemoprotective effect due to its capacity to inhibit CYP activity.
Asunto(s)
Antimutagênicos/farmacología , Antioxidantes/farmacología , Citocromo P-450 CYP1A1/antagonistas & inhibidores , Hígado/efectos de los fármacos , Mangifera , Microsomas Hepáticos/efectos de los fármacos , Extractos Vegetales/farmacología , Animales , Bleomicina , Carcinógenos , Cuba , Ciclofosfamida , Hígado/metabolismo , Microsomas Hepáticos/metabolismo , Pruebas de Mutagenicidad , Mutágenos , Corteza de la Planta , Tallos de la Planta , Ratas , Ratas Sprague-DawleyRESUMEN
The effect of transfluthrin (TF) or D-allethrin (DA) pyrethroid (PYR) vapors, often contained as main ingredients in two commercially available mosquito repellent mats, on cytochrome P450 (CYP) enzymes of rat brain and liver was assessed. Immunodetection of CYP2E1 and CYP3A2 proteins revealed their induction in cerebrum and cerebellum, but not in liver microsomes of rats exposed by inhalation to TF or DA. This overexpression of proteins correlated with an increase of their catalytic activities. The specifically increased expression of CYP isoenzymes, due to PYR exposure in the rat brain, could perturb the normal metabolism of endogenous and xenobiotic compounds and leads to increased risks of neurotoxicity by bioactivation, lipid peroxidation and DNA damage.
Asunto(s)
Encéfalo/efectos de los fármacos , Sistema Enzimático del Citocromo P-450/metabolismo , Mosquiteros Tratados con Insecticida , Insecticidas/toxicidad , Piretrinas/toxicidad , Aletrinas/química , Aletrinas/toxicidad , Animales , Western Blotting , Encéfalo/enzimología , Ciclopropanos/química , Ciclopropanos/toxicidad , Citocromo P-450 CYP2E1/metabolismo , Citocromo P-450 CYP3A/metabolismo , Electroforesis en Gel de Poliacrilamida , Fluorobencenos/química , Fluorobencenos/toxicidad , Exposición por Inhalación , Mosquiteros Tratados con Insecticida/efectos adversos , Insecticidas/química , Peroxidación de Lípido/efectos de los fármacos , Hígado/efectos de los fármacos , Hígado/enzimología , Masculino , Proteínas de la Membrana/metabolismo , Microsomas/efectos de los fármacos , Microsomas/enzimología , Síndromes de Neurotoxicidad/enzimología , Síndromes de Neurotoxicidad/etiología , Piretrinas/química , Ratas , Ratas Wistar , VolatilizaciónRESUMEN
Arnica (Heterotheca inuloides) is a widely used medicinal plant in México; it has been recognized as anti-inflammatory, analgesic, cytotoxic, scavenger of superoxide anion and also as a preventive of lipid peroxidation. In vivo studies have demonstrated a hepatoprotective action of the methanolic extract of this plant as well as of quercetin, one of its main components, and the evidence obtained pointed out to an antioxidant mechanism. In this work, we focused on the free radical scavenging capacity of acetonic and methanolic extracts of H. inuloides in comparison with reference compounds. The two extracts were 2-12 times more effective (IC50, microg/mL) than the reference compounds to cope with the following radicals or molecules tested: 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS(+)), 2,2-diphenyl-1-picrylhydrazyl (DPPH), peroxynitrite (ONOO(-)), superoxide (O2(-)), singlet oxygen ((1)O(2)), hypochlorous acid (HOCl), hydrogen peroxide (H2O2), hydroxyl (OH). Additionally, five secondary metabolites isolated from the methanolic extract displayed potent concentration-dependent antioxidant effects against reactive oxygen species produced in vitro (IC50 values in the range of 0.018-4.31mg/mL). d-Chiro-inositol showed the higher antioxidant effect against O2(-), H2O2 and OH while spinasterol and quercetin were the most active against (1)O(2) and ONOO(-), respectively. The antioxidant properties of the extracts and metabolites tested partially support the wide use of this plant in traditional medicine.
Asunto(s)
Antioxidantes/farmacología , Asteraceae/química , Extractos Vegetales/farmacología , Especies Reactivas de Oxígeno/metabolismo , Antioxidantes/administración & dosificación , Antioxidantes/aislamiento & purificación , Relación Dosis-Respuesta a Droga , Peróxido de Hidrógeno/metabolismo , Concentración 50 Inhibidora , Medicina Tradicional , México , Extractos Vegetales/administración & dosificación , Quercetina/administración & dosificación , Quercetina/aislamiento & purificación , Quercetina/farmacología , Estigmasterol/administración & dosificación , Estigmasterol/análogos & derivados , Estigmasterol/aislamiento & purificación , Estigmasterol/farmacologíaRESUMEN
Biotin deficiency and biotin excess have both been found to affect reproduction and cause teratogenic effects. In the reproductive tract, however, the effects of biotin have not been well established yet. We investigated the effects of varying biotin content diets on the oestrus cycle, ovarian morphology, estradiol and progesterone serum levels, and the uterine mRNA abundance of their nuclear receptors, as well as on the activity of the estradiol-degrading group of enzymes cytochrome P450 (CYP) in the liver. Three-week-old female BALB/cAnN Hsd mice were fed a biotin-deficient, a biotin-control, or a biotin-supplemented diet (0, 7.2 or 400 micromol of free biotin/kg diet, respectively) over a period of nine weeks. Striking effects were observed in the biotin-deficient group: mice showed arrested estrous cycle on the day of diestrus and changes in ovary morphology. Estradiol serum concentration increased 49.2% in biotin-deficient mice compared to the control group, while the enzymatic activities of CYP1A2 and CYP2B2 increased (P<0.05). The mRNA abundance of nuclear estrogen and progesterone receptors decreased in the biotin-deficient mice. In the biotin-supplemented group we found that, in spite of a significant (P<0.05) decrease in the number of primary and Graafian follicles and in CYP1A2 activities, mice exhibited 105.4% higher serum estradiol concentration than the control group. No changes in the expression of the nuclear receptors were observed. No significant differences were observed in serum progesterone among the groups. Our results indicate that both the deficiency and the excess of biotin have significant effects on the female mouse reproductive system.
Asunto(s)
Biotina/deficiencia , Biotina/farmacología , Reproducción/efectos de los fármacos , Reproducción/fisiología , Animales , Biotina/administración & dosificación , Biotina/sangre , Peso Corporal/efectos de los fármacos , Dieta , Estradiol/sangre , Ciclo Estral/efectos de los fármacos , Femenino , Hígado/anatomía & histología , Hígado/efectos de los fármacos , Hígado/enzimología , Ratones , Ratones Endogámicos BALB C , Tamaño de los Órganos/efectos de los fármacos , Ovario/anatomía & histología , Ovario/efectos de los fármacos , Progesterona/sangre , ARN Mensajero/genética , ARN Mensajero/metabolismo , Receptores de Estradiol/genética , Receptores de Progesterona/genética , Útero/efectos de los fármacos , Útero/metabolismoRESUMEN
Ionizing radiation causes different types of genetic damage, ranging from base modifications to single- and double-stranded DNA breaks, which may be deleterious or even lethal to the cell. There are different repair or tolerance mechanisms to counteract the damage. Among them is the Escherichia coli SOS system: a set of genes that becomes activated upon DNA damage to confer better opportunities for cell survival. However, since this response is triggered by single-stranded DNA regions, most lesions have to be processed or modified prior to SOS activation. Several genes such as recO, recB and recJ that seem to be required to induce the response have already been reported. The results of this work indicate that the four known E.coli single-strand exonucleases take part in processing gamma radiation damage, though RecJ and ExoI proved to be more important than ExoVII or ExoX. In addition, ExoV as well as glycosylases such as Nth and, to a lesser extent, Fpg are also required. A model intended to explain the role of all these genes in damage processing is presented.