RESUMEN
It is known that double-stranded DNA (dsDNA) turns into a liquid crystalline phase by the addition of a high concentration of polymer with salt. SYBR Green I (SG) is a well-known sensitive fluorescent stain for dsDNA, and is intercalated in liquid crystalline DNA. Formation of the liquid crystalline dsDNA-SG complex has been confirmed by CD spectral measurements, fluorescence spectral measurements and confocal fluorescence microscopy. SG in dsDNA was also used as a singlet oxygen generator. We conducted photoirradiation experiments using three kinds of 42-mer oligonucleotides with SG. The amount of guanine decomposition by selective irradiation of SG was analyzed using HPLC after digestion of dsDNA in each sample solution. We found that singlet oxygen produced in liquid crystalline DNA promoted guanine damage much more efficiently than in homogeneous solution.
Asunto(s)
Oxígeno SingleteRESUMEN
Spermidine, a trivalent organic cation, induced DNA structural changes and suppressed guanine photooxidative decomposition via electron transfer through pyrene-modified DNA. On the other hand, adding higher concentrations of spermidine resulted in DNA condensation. The efficiency of guanine decomposition in condensed pyrene-modified DNA was promoted remarkably.
Asunto(s)
ADN/química , Guanina/química , Espermidina/química , Transporte de Electrón , Procesos FotoquímicosRESUMEN
We investigated the effects of a crowded environment on guanine oxidation in pyrene-modified oligonucleotides by photoinduced electron transfer. We observed drastic promotion of guanine oxidation in Ψ (psi; polymer- and salt-induced)-type DNA.