Orchestration of macrophage polarization dynamics by fibroblast-secreted exosomes during skin wound healing.

Macrophages undertake pivotal yet dichotomous functions during skin wound healing, mediating both early pro-inflammatory immune activation and late anti-inflammatory tissue remodeling processes. The timely phenotypic transition of macrophages from inflammatory M1 to pro-resolving M2 activation states is essential for efficient healing. However, the endogenous mechanisms calibrating macrophage polarization in accordance with the evolving tissue milieu remain undefined. Here, we reveal an indispensable immunomodulatory role for fibroblast-secreted exosomes in directing macrophage activation dynamics. Fibroblast exosomes permitted spatiotemporal coordination of macrophage phenotypes independent of direct intercellular contact. Exosomes enhanced macrophage sensitivity to both M1 and M2 polarizing stimuli, yet also accelerated timely switching from M1 to M2 phenotypes. Exosomes inhibition dysregulated macrophage responses resulting in aberrant inflammation and impaired healing, while provision of exogenous fibroblast exosomes corrected defects. Topical application of fibroblast exosomes onto chronic diabetic wounds normalized dysregulated macrophage activation to resolve inflammation and restore productive healing. Our findings elucidate fibroblast-secreted exosomes as remote programmers of macrophage polarization that calibrate immunological transitions essential for tissue repair. Harnessing exosomes represents a previously unreported approach to steer productive macrophage activation states with immense therapeutic potential for promoting healing in chronic inflammatory disorders.

Spatiotemporal orchestration of macrophage activation trajectories by Vγ4 T cells during skin wound healing.

Dysregulated macrophage polarization from pro-inflammatory M1 to anti-inflammatory M2 phenotypes underlies impaired cutaneous wound healing. This study reveals Vγ4+ γδ T cells spatiotemporally calibrate macrophage trajectories during skin repair via sophisticated interferon-γ (IFN-γ) conditioning across multiple interconnected tissues. Locally within wound beds, infiltrating Vγ4+ γδ T cells directly potentiate M1 activation and suppress M2 polarization thereby prolonging local inflammation. In draining lymph nodes, infiltrated Vγ4+ γδ T cells expand populations of IFN-γ-competent lymphocytes which disseminate systemically and infiltrate into wound tissues, further enforcing M1 macrophages programming. Moreover, Vγ4+γδ T cells flushed into bone marrow stimulate increased IFN-γ production, which elevates the output of pro-inflammatory Ly6C+monocytes. Mobilization of these monocytes continually replenishes the M1 macrophage pool in wounds, preventing phenotypic conversion to M2 activation. Thus, multi-axis coordination of macrophage activation trajectories by trafficking Vγ4+ γδ T cells provides a sophisticated immunological mechanism regulating inflammation timing and resolution during skin repair.

Accumulation and migration of particulate trace metals by artificial flood event of the Yellow River: From Xiaolangdi reservoir to estuary.

Reservoir construction increasingly alters the natural transport of riverine water and sediment to the sea, including the trace metals and other pollutants. In 2018, an intensive flood event and 412 million tons of sediment were released from the Xiaolangdi dam during the water-sediment regulation of Yellow River, one of the world's largest sediment releases. During the artificial flood event, the surface sediments in Xiaolangdi Reservoir (XLD) and the Yellow River estuary, suspended sediments at Lijin Station were collected. The concentration and speciation of particulate Cr, Ni, Cu, Zn, Cd and Pb, as well as the major geochemical composition were analyzed, to characterize the behaviors of the metals from the reservoir to estuary mixing zone, and elucidate the controlling mechanisms. The results showed that for exogenous phases, Cr, Ni, Cu and Zn were likely bound to the FeMn oxides, whereas Pb and Cd were mainly adsorbed in the carbonates. The trace metals in XLD were stably combined with fine-grained bottom sediments at high concentrations before dam release. During the delivery from reservoir to downstream channel, the binding of Cr, Ni and Cu with FeMn oxides was markedly enhanced. Pb and Cu showed obvious migration from carbonates to FeMn oxides, and Cd and Pb were even released into the water. The accumulation and migration of trace metals were controlled by the adsorption of fine-grained components, especially FeMn oxides and carbonates, and influenced by the oxidizing processes. After entering the estuary, the trace metals were greatly scavenged by reservoir-sourced fine particles, tended to bound to organic matter affected by the reducing environment. Our results suggest that dam regulation and artificial flood events will likely alter the existing forms and redox state of trace metals and the potential environmental effects should be considered.

Heavy metals in the sediments of Laoshan Bay, China: Distribution and contamination assessment.

We analyzed total organic carbon (TOC), total nitrogen (TN), and heavy metals in 58 sediment samples from the Laoshan Bay, China. TOC and TN results identified marine primary production as the dominant source for organic matter, and the weak correlations between TOC, TN, and heavy metals indicated the terrestrial origin of heavy metals. Cd showed insignificant correlations with other metals, which suggests that agriculture production is the main anthropogenic activity affecting the distribution of Cd. Pb contamination in past 50 years was likely to have occurred because of fossil fuel combustion. Despite 210Pb dating, it was still difficult to trace the contamination condition over interannual variations. Nonetheless, these results provide data for monitoring Pb contamination. As Laoshan Bay is an important economic area, it would be beneficial to conduct further studies to determine the sources and degree of contamination for each heavy metal.

Variability of heavy metal transport during the water-sediment regulation period of the Yellow River in 2018.

To understand the impacts of the human-induced flood event on heavy metal (HM) transport, spatiotemporal variations in contents and fluxes of metals (Cr, Ni, Cu, Zn, As, Pb, Cd), Pb stable isotopes and characteristics of water and sediment transport into the sea during Water-Sediment Regulation Scheme (WSRS) in Yellow River (YR) were studied based on field investigation at Xiaolangdi Reservoir (XLD), Lijin Station and Yellow River estuary (YRE). The HM transport was significantly controlled by hydrological process and dominated by particulate form with strong associations with particle size and suspended sediment concentration (SSC). In first stage, dissolved heavy metal (DHM) and particulate heavy metal (PHM) contents both increased significantly as coarser sediment with a mixed source of downstream river channel and XLD, while that maintained higher value for stable source of fine-grained XLD sediment in second stage. The HMs into the sea were mainly originated from upper and middle reaches but also contributed by human emissions from downstream area. As the source of HMs into the sea, the downstream area also acted as an important sink, especially in first stage, playing a role of buffering and filtration. During WSRS, the YR discharged 49%-60% of annual HM flux into the sea, and the second stage is the main transport period, leading to a great alternation in geochemical composition of the YRE sediment.

Budget and fate of sedimentary trace metals in the Eastern China marginal seas.

To achieve a better understanding of global biogeochemical cycle and budget of trace metals (TM) in the world's ocean, a comprehensive study of the level, fate and burial flux of TM in surface sediment from the Eastern China Marginal Seas (ECMS) was conducted. The results indicated that Pb, Zn, Cu, and Cr were highly concentrated in mud deposits and primarily controlled by the natural processes of sediment composition and regional hydrodynamics, whereas As had a close association with Mn and was mainly derived from anthropogenic activity. The sediment mass inventories of Pb, Zn, Cu, Cr, and As in the ECMS (~220,780 km2) were estimated to be 28,324, 92,192, 23,434, 94,560 and 11,968 t/yr, respectively. A mass budget model revealed that riverine runoff, coastal erosion input and atmospheric deposition (dry and wet) constituted 62-76%, 15-37% and 2-9% of the total Pb, Zn and Cr influxes, respectively, while more than 4,690 t of Cu annually outflowed to the open seas to balance the budget. More importantly, we found that the sum of the estimates of these contributions tended to fall short of the calculated depositional fluxes of As, implying that anthropogenic activities probably have altered the natural geochemical cycle of As. Our results suggest that the ECMS constitutes a major final repository of TM at the Asia scale; however, the burial fluxes of trace metals are expected to decrease due to enhanced environmental investments by the Chinese government and decreased suspended particulate TM loads from the Chinese major rivers.

Accumulation and transformation of heavy metals in surface sediments from the Yangtze River estuary to the East China Sea shelf.

The concentration and speciation of heavy metals (Cu, Co, Ni, Zn, Cr, Pb and Cd) were studied in surface sediment from the Yangtze River(YR)to the East China Sea (ECS) shelf. The results showed that high contents of metals were found in the YR estuary (YRE) and in the nearshore muddy area, while lower concentrations were found in the YR channel and the ECS shelf. However, after standardization, the total content of most heavy metals from the YR showed little change or slightly increased during the transport process from the river to the estuary but decreased significantly outside the estuary, especially in the sediments of the ECS shelf. The residual fraction is the dominant fraction for all the metals, while the oxidizable and reducible fractions are the most important forms of the nonlithogenic fractions. The total amount of heavy metals from the YR to the continental shelf is mainly affected by the filtration of the estuary and the barrier impacts of the coastal current in the ECS. The environmental physicochemical conditions that vary significantly in the turbidity zone greatly influence the associated forms of metals. The metals in the acid-soluble fraction are mostly affected by the pH change in the sediment and the discharge of human activities, while the reducible fraction is significantly affected by the bottom water DO. The oxidizable fraction was affected by oxidation-reduction potential (ORP), primary productivity, as well as OM content. Therefore, with changes in the physicochemical conditions of the environment, the metals have undergone significant changes in their speciation from the YR to the ECS shelf. Various complex effects in the estuary area have not only a large filtration effect on the total amount of metals but also a major impact on the geochemical forms of the metals.

Persistent impact of human activities on trace metals in the Yangtze River Estuary and the East China Sea: Evidence from sedimentary records of the last 60 years.

Three sediment cores taken from the Yangtze River Estuary (YRE) and the East China Sea (ECS) in 2016 were analyzed for trace metals (Pb, Zn, Cu, Cr and As), major elements (Al, Fe and Mn), sediment composition, chemical properties (Eh and pH), and natural/artificial radionuclides (210Pb/137Cs) to decipher the high-resolution historical variation in anthropogenic metals over the past 60 years. The results showed that anthropogenic Pb was primarily derived from atmospheric deposition, while anthropogenic Zn, Cu and As were carried out by fluvial discharge. In the YRE, the recent decrease in sedimentary metals could be largely a result of intensified erosion, which was triggered by the construction of dams in particular the Three Gorges Dam (TGD) in 2003 and soil-conservation projects. In comparison, sedimentary records in the deposition-dominated environment of the ECS showed that anthropogenic Pb and Cr began to gradually increase following 1970, but a remarkable increase in anthropogenic Zn and Cu occurred in 1990, probably reflect the different origins and dispersion pathways of these elements. Anthropogenic Pb and Cr exhibited a sharp decrease near early 2000s due to increasing investment in treating pollution. Being sensitive to the substantial reduction in riverine particulate flux of Zn and Cu from the Yangtze River, the concentrations of anthropogenic Zn and Cu in the cores decreased by 5-27% in 2005-2016. Furthermore, a prominent increase trend of As record after 1990, probably due to the excessive pesticides used in agricultural activity.

Impact of water-sediment regulation on the transport of heavy metals from the Yellow River to the sea in 2015.

Variations in particulate and dissolved heavy metal (Cu, Co, Ni, Zn, Pb, Cr, Cd and As) concentrations and fluxes as well as their response to the Water-Sediment Regulation Scheme (WSRS) process in 2015 were studied based on a daily water and sediment survey at Lijin gauge. The results showed that the water and sediment flux increased rapidly in the first stage of the WSRS, which was characterized by high water discharge and suspended sediment concentration (SSC). The suspended sediment was coarser than the natural state, while the particulate and dissolved heavy metal contents increased. In the second stage, the SSC decreased rapidly followed by a gradual reduction in water discharge, the suspended sediment became even coarser and both the particulate and dissolved metal contents showed a decreasing trend. The heavy metal flux during the WSRS period accounted for 42%-54% of the total year, and the transport form was dominated by the particulate form. Dissolved metal contents were affected by the release of heavy metals derived from the channel in the lower reaches, while particulate heavy metals mainly came from erosion of the riverbed and their contents were much lower than Xiaolangdi reservoir sediment. Heavy metal transportation was influenced significantly by the WSRS process. Changes in sediment flux resulted in significant differences in the flux of heavy metals and the distribution of metals in different transport forms between the first and second stage of WSRS.

Source identification, geochemical normalization and influence factors of heavy metals in Yangtze River Estuary sediment.

Sediment samples, including 40 surface samples and 12 sediment cores, were collected from 52 stations of the Yangtze River Estuary (YRE) in 2015 and 2016. The 95% linear prediction intervals (LPI) and principal components analysis (PCA), were conducted to evaluate the metal sources and grain-size effect (GSE). The in situ physico-chemical properties of pH, Eh, DO, salinity, temperature and turbidity were combined to elucidate the relationships between environmental factors and the fate of heavy metals in the river-estuary-shelf system. This study indicates a decreasing trend of metals in sediments from the estuary towards the adjacent shelf and the river channel and that Zn, Cu and Cr are mainly derived from natural processes throughout the catchment, whereas Pb appears to have anthropogenic inputs via atmospheric deposition. Furthermore, considering the best fit regression lines between the concentrations of Al and heavy metals as well as the deficiencies of the conventional Celements/CAl method, we introduce an approach (Al-SN: Al-scope normalization) that can eliminate the GSE on heavy metals and be applied to other estuaries. After Al-scope normalization, the relatively constant levels of Zn, Cu and Cr that remain in sediments from the river channel to the estuary and shelf confirmed that the variation of grain size in sediments almost entirely explained the distribution patterns of sediment toxicity in the YRE, while the enrichment of Pb in estuarine sediments could be attributed to its chemical species and physico-chemical properties. The results further suggest that the relationship between grain size and spatial behavior of sediment pollutants should be given priority over the contamination assessment and provenance discrimination in estuarine or similar environments with complex sediment compositions.

Spatio-temporal distribution and sources of Pb identified by stable isotopic ratios in sediments from the Yangtze River Estuary and adjacent areas.

To understand the spatio-temporal distribution and sources of Pb in the sediments of the Yangtze River Estuary and its adjacent areas, 25 surface sediments and 1 sediment core were collected from the study areas. The concentrations of Al and Pb of these sediments exhibit a decreasing trend from the nearshore towards the offshore, with higher concentrations in the coastal areas of the East China Sea (ECS) and southwest of Jeju Island. According to the stable isotopic ratios of Pb, in combination with the elemental ratios and clay mineral data, it is inferred that sedimentary Pb in the surface sediments of the coastal areas of the ECS may come primarily from the Yangtze River, while the Pb southwest of Jeju Island is probably derived from both the Yangtze and Yellow Rivers. The particulate Pb derived from the Yangtze River was possibly dispersed along two paths: the path southward along the coastline of the ECS and the path eastward associated with the Changjiang Diluted Water (CDW), which crosses the shelf of the ECS towards the area southeast of Jeju Island. Although the Yangtze River Basin witnessed rapid economic development during the period from the late 1970s to the middle 1990s, the influence of human activity on Pb concentration remained weak in the Yangtze River Estuary. Since the early 2000s, however, sedimentary Pb has been significantly increasing in the coastal mud areas of the ECS due to the increasing influence of human activity, such as the increase in atmospheric emission of anthropogenic Pb in China, construction of the Three Gorges Dam (TGD), and the construction of smaller dams in the upper reaches of the Yangtze River. Coal combustion and the smelting of non-ferrous metals are possible anthropogenic sources for the sedimentary Pb in the Yangtze River Estuary.

Distribution and sources of polycyclic aromatic hydrocarbons in surface sediments from the Bering Sea and western Arctic Ocean.

To analyze the distribution and sources of polycyclic aromatic hydrocarbons (PAHs) and evaluate their potential ecological risks, the concentrations of 16 PAHs were measured in 43 surface sediment samples from the Bering Sea and western Arctic Ocean. Total PAH (tPAH) concentrations ranged from 36.95 to 150.21 ng/g (dry weight). In descending order, the surface sediment tPAH concentrations were as follows: Canada Basin>northern Chukchi Sea>Chukchi Basin>southern Chukchi Sea>Aleutian Basin>Makarov Basin>Bering Sea shelf. The Bering Sea and western Arctic Ocean mainly received PAHs of pyrogenic origin due to pollution caused by the incomplete combustion of fossil fuels. The concentrations of PAHs in the sediments of the study areas did not exceed effects range low (ERL) values.

The environment quality of heavy metals in sediments from the central Bohai Sea.

The heavy metals (Cu, Co, Ni, Zn, Cr, Pb, Cd) in surface and core sediments from the central Bohai Sea were analyzed to evaluate the temporal/spatial distribution and pollution status. Cd exhibited gradual increase vertically, while others were stable or declined slightly in core sediments. In surface sediments, metals showed higher values in 'central mud area of the Bohai Sea' and the coastal area of the Bohai Bay. Cd and Pb also had high levels in the northeastern part of Bohai Sea. Both the contamination factors (CFs) and the geo-accumulation index (Igeo) indicated that Cu, Co, Ni, and Cr were not at pollution levels, while Pb, Zn, and Cd indicated moderate contamination. Compared with sediment quality guidelines (SQGs), Cu, Zn, Cr, Pb, and Cd were likely to produce occasional adverse biological effects, while Ni showed possible ecotoxicological risks. The combined levels of the metals have a 21% probability of being toxic.

Spatial distribution of organic and pyritic sulfur in surface sediments of eutrophic Jiaozhou Bay, China: clues to anthropogenic impacts.

Anthropogenic perturbations exert important impacts on sulfur geochemistry in marine sediments. In the study, chemical extraction was used to quantify four sulfur pools, i.e., pyrite, humic-acid sulfur (HA-S), fulvic-acid sulfur (FA-S), and residual organic sulfur (ROS), in surface sediments of eutrophic Jiaozhou Bay. Results show that riverine inputs are the main control on organic matter (OM) distribution in the sediments. OM enrichment in the eastern coast is mainly due to discharges of anthropogenic wastes. Spatial coupling of pyrite and FA-S vs. TOC points to the impacts of OM enrichment on formation and preservation of pyrite and FA-S. Poor spatial coupling of HA-S vs. TOC is due to low fractions of diagenetic OS in the pool. ROS is mainly from riverine inputs and anthropogenic OS has been superimposed on this pool. Spatial coupling among TOC, pyrite-S and FA-S is a sensitive indicator of anthropogenic impacts on benthic processes of the bay.

[Distribution patterns of heavy metals in surface sediments of the Yangtze estuary and its adjacent areas and environmental quality assessment].

The concentrations of six heavy metals were determined in the fine fraction ( < 63 microm) of surface sediments in the Yangtze Estuary and its adjacent areas by an inductively coupled plasma mass spectroscopy (ICP-MS). The average contents of As, Cd, Cu, Pb, Cr and Zn are 10.47, 0.19, 37.68, 36.86, 97.80 and 98.65 mg x kg(-1), respectively. The contents of As, Cd and Cu appear gradually degressive from west to east, while the contents of Pb, Cr and Zn appear no apparent difference. In generally, the concentrations of heavy metals are higher in the south sea areas than those in the north sea areas. Environmental assessment with single factor method suggests that As, Cu, Pb, Cr and Zn are moderately contaminated, while Cd is not polluted. The values of As,Cu and Cr are between effect range-low (ERL) and effect range-median (ERM), which indicate they occasionally bring adverse biological effect at most stations, and the values of Cd,Pb and Zn are below ERL mean they rarely bring adverse biological effect by sediment quality guidelines assessment. From the results of factor analysis and cluster analysis, we conclude that the outer sea areas of the Yangtze Estuary and the Hangzhou Bay have lower sediment environmental quality, therefore much attention should be paid to these areas.

Tracking historical lead pollution in the coastal area adjacent to the Yangtze River Estuary using lead isotopic compositions.

The rapid economic development in the Yangtze River Delta (YRD), China in the last three decades has had a significant impact on the environment of the East China Sea (ECS). Lead isotopic compositions of a (210)Pb dated sediment core collected from the coastal ECS adjacent to the Yangtze River Estuary were analyzed to track the Pb pollution in the region. The baseline Pb concentration in the coastal ECS sediments before the industrialization in China was 32 microg g(-1), and the corresponding 206Pb/207Pb ratio was 1.195. The high-resolution profiles of Pb flux and 206Pb/207Pb ratios had close relationships with the economic development and the history of the use of leaded gasoline in China, and they were clearly different from those of most European countries and United States.

[Distribution of suspended matter concentration in the Changjiang Estuary and adjacent area after the first-stage storage of the Three Gorges Project].

The first-stage storage of the Three Gorges Project started on the 1st June, 2003 and ended on the 10th June, 2003. In order to investigate the influences of the Three Gorges Project on the Changjiang Estuary and adjacent area environment, a cruise was carried out from the 15th June, 2003 to the 25th June, 2003 to synthetically study the environment. Suspended matter concentrations (SMC) in different water layers at different sites were calculated and distributions of SMC were discussed combining with salinity and temperature of water based on recovered data. In results, SMC increase from surface to bottom in Changjiang Estuary and adjacent area. Suspended matters are enriched in the water over the river mouth bar and alongshore area which are located at the west of 123 degrees E. Compared to that before the storage, SMC are reduced obviously in the Changjiang course and south branch after the storage. For example, at the Xuliujing, SMC are reduced from 400 - 500 mg/L to 60.2 mg/L, and in the south branch, from 445 mg/L to 148 mg/L. In other areas, SMC do not show a significant change that they are both less than 10 mg/L in the surface player and 100 mg/L in bottom player. Generally, the distributed patterns of SMC in the investigation area after the storage are still similar to that before the storage.