RESUMEN
Since May 2022, the multi-country outbreak of monkeypox (mpox) has raised a great concern worldwide. Early detection of mpox virus infection is recognized as an efficient way to prevent mpox transmission. Mpox specific detection methods reported up to now are based on the SNPs among mpox virus and other orthopoxviruses. We have therefore developed a real-time PCR based mpox detection method targeting mpox virus specific sequences (N3R and B18Rplus). We have also optimized an orthopoxvirus detection system which targets the highly conserved E9L and D6R genes. The mpox and orthopoxvirus real-time PCR assays have a high sensitivity (1 copy/reaction) and specificity. Mpox viral DNA and clinical samples from mpox patients are detected with the mpox detection system. Furthermore, we have established a multiplex real-time PCR detection system allowing simultaneous and efficient detection of mpox and orthopoxvirus infections.
Asunto(s)
Monkeypox virus , Mpox , Reacción en Cadena de la Polimerasa Multiplex , Orthopoxvirus , Infecciones por Poxviridae , Reacción en Cadena en Tiempo Real de la Polimerasa , Sensibilidad y Especificidad , Orthopoxvirus/genética , Orthopoxvirus/aislamiento & purificación , Humanos , Reacción en Cadena en Tiempo Real de la Polimerasa/métodos , Reacción en Cadena de la Polimerasa Multiplex/métodos , Monkeypox virus/genética , Monkeypox virus/aislamiento & purificación , Infecciones por Poxviridae/diagnóstico , Infecciones por Poxviridae/virología , Infecciones por Poxviridae/veterinaria , Mpox/diagnóstico , Mpox/virología , Técnicas de Diagnóstico Molecular/métodosRESUMEN
Among persons born in China before 1980 and tested for vaccinia virus Tiantan strain (VVT), 28.7% (137/478) had neutralizing antibodies, 71.4% (25/35) had memory B-cell responses, and 65.7% (23/35) had memory T-cell responses to VVT. Because of cross-immunity between the viruses, these findings can help guide mpox vaccination strategies in China.