Contaminant removal performance and lipid productivity of a cyanobacteria-bacteria consortium containing exogenous phytohormones during the treatment of antibiotic-polluted wastewater.

In this study, a cyanobacteria-bacteria consortium containing native wastewater bacteria and immobilized Synechococcus sp. was constructed. The cyanobacterial cellular responses (including growth, biomass and lipid productivity) and contaminant removal ability (for TN, TP, COD and antibiotics) in the consortium were evaluated during the advanced treatment of wastewater containing 10-50 µg/L of mixed antibiotics (amoxicillin, tetracycline, erythromycin, sulfadiazine and ciprofloxacin) with the addition of a certain phytohormone (indole-3-acetic acid, gibberellin A3 or 6-benzylaminopurine) at trace level within a period of four days. Each phytohormone promoted the growth of Synechococcus sp. and increased the tolerance of Synechococcus sp. to mixed antibiotics. Indole-3-acetic acid coupled to moderate antibiotic stress could elevate lipid productivity and lipid content of Synechococcus sp. to 33.50 mg/L/day and 43.75%, respectively. Phytohormones increased the pollutant removal performance of the cyanobacteria-bacteria consortium through the stimulation of cyanobacterial growth and the regulation of cyanobacteria-bacteria interaction, which increased the abundances of microalgae-associated bacteria including Flavobacterium, Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium, Bosea, Sphingomonas and Emticicia. Up to 80.83%, 98.06%, 83.26%, 99.84%, 99.50%, 89.41%, 65.61% and 60.65% of TN, TP, COD, amoxicillin, tetracycline, erythromycin, sulfadiazine and ciprofloxacin were removed by the consortium with the addition of phytohormones. In general, indole-3-acetic acid was the optimal phytohormone for enhancing lipid production and contaminant removal performance of the cyanobacteria-bacteria consortium.

Advanced treatment of antibiotic-polluted wastewater by a consortium composed of bacteria and mixed cyanobacteria.

This study constructed a cyanobacteria-bacteria consortium using a mixture of non-toxic cyanobacteria (Synechococcus sp. and Chroococcus sp.) immobilized in calcium alginate and native bacteria in wastewater. The consortium was used for the advanced treatment of sulfamethoxazole-polluted wastewater and the production of cyanobacterial lipid. Mixed cyanobacteria increased the abundances of denitrifying bacteria and phosphorus-accumulating bacteria as well as stimulated various functional enzymes in the wastewater bacterial community, which efficiently removed 70.01-71.86% of TN, 91.45-97.04% of TP and 70.72-76.85% of COD from the wastewater. The removal efficiency of 55.29-69.90% for sulfamethoxazole was mainly attributed to the upregulation of genes encoding oxidases, reductases, oxidoreductases and transferases in two cyanobacterial species as well as the increased abundances of Stenotrophomonas, Sediminibacterium, Arenimonas, Novosphingobium, Flavobacterium and Hydrogenophaga in wastewater bacterial community. Transcriptomic responses proved that mixed cyanobacteria presented an elevated lipid productivity of 33.90 mg/L/day as an adaptive stress response to sulfamethoxazole. Sediminibacterium, Flavobacterium and Exiguobacterium in the wastewater bacterial community may also promote cyanobacterial lipid synthesis through symbiosis. Results of this study proved that the mixed cyanobacteria-bacteria consortium was a promising approach for advanced wastewater treatment coupled to cyanobacterial lipid production.

Mechanisms for the increase in lipid production in cyanobacteria during the degradation of antibiotics.

This study evaluated the responses of cell density, photosynthesis activity, dry cell weight, lipid productivity, proteome and metabolome in two non-toxic cyanobacterial species (Synechococcus sp. and Chroococcus sp.) exposed to two frequently detected antibiotics (sulfamethoxazole and ofloxacin) at test concentrations of 0.2-20.0 µg L-1 in a 4-day culture period. Upregulated antioxidant enzymes and oxidoreductases contributed to antibiotic biodegradation in Synechococcus sp.; whereas, upregulated carotenoid protein contributed to antibiotic biodegradation in Chroococcus sp. The 4-day removal efficiencies of sulfamethoxazole and ofloxacin by cyanobacteria were 35.98-66.23% and 33.01-61.92%, respectively. In cyanobacteria, each antibiotic induced hormetic responses, such as increase in cell density, dry cell weight, and photosynthetic activity; upregulation of photosynthesis-related proteins; and elevation of lipid expression by up to 2.05-fold. Under antibiotic stress, the two cyanobacterial species preferred to store energy in the form of lipids rather than ATP, with fructose-bisphosphate aldolase playing an essential role in lipid synthesis. The downregulation of lipid transporters also facilitated lipid accumulation in Synechococcus sp. In general, the two non-toxic cyanobacterial species achieved a good combination of lipid deposition and antibiotic treatment performance, especially in Chroococcus sp. exposed to sulfamethoxazole.

Production of struvite by magnesium anode constant voltage electrolytic crystallisation from anaerobically digested chicken manure slurry.

Nitrogen and phosphorus levels in livestock manure and digestive fluid are high, posing a threat to soil and water quality and necessitating nutrient removal and recovery. Phosphorus recovery has the potential to alleviate the global phosphorus resource crisis. This study proposed a magnesium anode constant voltage electrolysis method to crystallise struvite (magnesium ammonium phosphate hexahydrate, MgNH4PO4·6H2O) from anaerobically digested chicken manure slurry using reaction kinetics at variable constant voltages ranging from 2 V to 12 V. The recovery of nitrogen and phosphorus was shown to be effective over a wide initial pH range (3.00 ± 0.03-7.90 ± 0.10) using synthetic digestion fluids. Moreover, the pH gradually increased during the reaction without any external chemical adjustments. The phosphorus recovery rates conformed to the first-order kinetic model, with a maximum rate constant of 2.13 h-1. When the best voltage of 2 V was used at 25 ± 1 °C, the recovery rate reached 5.24 mg P h-1cm-2 in the synthetic digestion fluids during 90 min and 4.60 mg P h-1cm-2 in the anaerobically digested chicken manure slurry. The crystalline products recovered were identified as high-purity struvite by XRD and XPS. The purity of recovered struvite with an initial pH of 3.00 and 7.90 was 96.5% and 98.9%, respectively. These results demonstrated that the magnesium electrode could rapidly react with nitrogen and phosphorus to generate high-purity struvite.

Community succession during the preventive control of cyanobacterial bloom by hydrogen peroxide in an aquatic microcosm.

Hydrogen peroxide (H2O2) is an environmentally friendly algaecide with good prospects for cyanobacterial bloom control. In this study, 0.2-1.5 mg L-1 of H2O2 was applied to an aquatic microcosm containing cyanobacteria, bacteria, and eukaryotic phytoplankton at the early cyanobacterial growth stage·H2O2 generated hormesis in cyanobacteria at 0.2 mg L-1; significantly (p < 0.05) inhibited cyanobacterial growth, cyanobacterial photosynthesis, and microcystin production at 0.5-1.5 mg L-1; and effectively prevented the formation of cyanobacterial bloom without generating adverse effects on eukaryotic phytoplankton at 1.0 and 1.5 mg L-1. Application of 0.5-1.5 mg L-1 H2O2 directly inhibited the abundance of five typical bloom-forming cyanobacterial genera (Microcystis, Anabeana, Synechococcus, Nostoc, and Oscillatoria), which were negatively correlated with four bacterial genera (Actinotalea, Flavobacterium, Fluviicola, and Exiguobacterium) and five eukaryotic phytoplankton genera (Cyclotella, Desmodesmus, Dinobryon, Fragilaria, and Mychonastes) and positively correlated with six proteobacterial genera (Brevundimonas, Devosia, Limnohabitans, Porphyrobacter, Pseudomonas, and Rhodobacter). After application of 1.0 and 1.5 mg L-1 H2O2 for 15 days, H2O2-treated groups showed significantly (p < 0.05) different prokaryotic community structures from that of the control group at the bloom stage (15th day), while eukaryotic community structures in H2O2-treated groups remained stable and showed high similarity with that of the control group at a non-bloom stage (5th day). Application of low-dose H2O2 during the early cyanobacterial growth stage could effectively prevent the formation of cyanobacterial blooms without disrupting non-target organisms.

Toxic effects of acetone, 2-pentanone, and 2-hexanone on physiological indices of wheat (Triticum aestivum L.) germination and seedlings.

Petroleum hydrocarbons are important characteristic pollutants in the process of oil exploitation in the Yellow River Delta (China), and they cause a potential hazard to the surrounding ecological environment. The research on eco-toxicological effects of petroleum-derived products still needs to be studied in depth. This paper describes the physiological indices of wheat (Triticum aestivum L.) seeds and seedlings under independent stresses of acetone, 2-pentanone, and 2-hexanone to determine the toxicological effects of ketones derived from petroleum products on typical crops. The experimental results indicated that ketones with concentrations lower than 0.4 mg·cm-2 and 800 mg·kg-1 the germination of wheat seeds and the growth of seedlings were promoted to 113.32-127.27% and 105.41-126.39%, respectively, thus exhibiting low-dose excitatory effects. However, when the concentration was higher than 0.4 mg·cm-2 and 800 mg·kg-1, germination and seedlings' growth were significantly reduced to 7.14-2.12% and 35.09-13.33%, respectively. At the same time, acetone had a greater impact on the growth of wheat seed roots, the malondialdehyde (MDA), and chlorophyll contents in leaf tissues. The low concentration of acetone had a significant promoting effect on the activity of α-amylase in wheat seeds. 2-Pentanone reduced the electrical conductivity of wheat seed extract, and it significantly promoted the catalase (CAT) activity at low concentrations. 2-Hexanone had a strong inhibitory effect on wheat germination and growth. This study provided new research results to determine the toxic effects of petroleum-derived products and provided a basis for the environmental management of such substances.