Potential pathological mechanisms and pharmacological interventions for cadmium-induced miscarriage.

The prevalence of cadmium (Cd) contamination has emerged as a significant global concern. Exposure to Cd during pregnancy is associated with adverse pregnancy outcomes, including miscarriage. However, there is currently a lack of comprehensive summaries on Cd-induced miscarriage. Therefore, it is imperative to further strengthen research into in vivo studies, clinical status, pathological mechanisms, and pharmacological interventions for Cd-induced miscarriage. This study systematically presents the current knowledge on animal models and clinical trials investigating Cd exposure-induced miscarriage. The underlying mechanisms involving oxidative stress, inflammation, endocrine disruption, and placental dysfunction caused by Cd-induced miscarriage are also extensively discussed. Additionally, potential drug interventions such as melatonin, vitamin C, and vitamin E are highlighted for their pharmacological role in mitigating adverse pregnancy outcomes induced by Cd.

Dityrosine Aggravates Hepatic Insulin Resistance in Obese Mice by Altering Gut Microbiota and the LPS/TLR4/NF-κB Inflammatory Pathway.

SCOPE: Dityrosine is the main product of protein oxidation, which has been proved to be a threat to human health. This study aims to investigate whether dityrosine exacerbates insulin resistance by inducing gut flora disturbance and associated inflammatory responses. METHODS AND RESULTS: Mice fed with normal diet or high-fat diet (HFD) received daily gavage of dityrosine (320 µg kg-1 BW) or saline for consecutive 13 weeks. The effects of dityrosine on gut microbiota are verified by in vitro fermentation using fecal microbiota from db/m mice and db/db mice. As a result, dityrosine causes the insulin resistance in mice fed normal diet, and aggravates the effects of HFD on insulin sensitivity. Dityrosine increases the levels of lipopolysaccharide (LPS), lipopolysaccharide-binding protein (LBP), toll-like receptor 4 (TLR4), nuclear factor kappa-B (NF-κB), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-8 (IL-8) but decreases levels of interleukin-10 (IL-10) in the plasma of CON and HFD-fed mice. The changes of gut flora composition caused by dityrosine are significantly correlated with the changes of inflammatory biomarkers. CONCLUSION: The effects of dityrosine on insulin resistance may be attributed to the reshaping of the gut microbiota composition and promoting the activity of the LPS/TLR4/NF-κB inflammatory pathway in HFD-induced obese individuals.

Finding the priority and cluster of inflammatory biomarkers for infectious preterm birth: a systematic review.

Infectious preterm birth (PTB) is one of the most important causes of perinatal death. It is difficult to find reliable biomarkers accurate to gestational weeks for infectious PTB prediction clinically. Infectious PTB is found usually accompanied with immune imbalance. Thus, the systematic study to find the priority of inflammatory biomarkers and innovative inflammatory clusters for infectious PTB prediction is urgently needed.This systematic study that focused on the inflammatory clusters and infectious PTB in the PubMed database was analyzed by using the criteria of the Population, Intervention, Comparison, Outcome, and Study design (PICOS) framework according to the recommendations of preferred reporting items for systematic reviews and meta-analysis (PRISMA).The network meta-analyzed results showed that the prioritization of the inflammatory factors for infectious PTB prediction is soluble tumor necrosis factor receptor 2 (sTNFR2) > tumor necrosis factor α (TNFα) > interleukin-10 (IL-10) > interleukin-6 (IL-6) > C-reactive protein (CRP) > interleukin-1ß (IL-1ß). Furthermore, the results also indicated that global consideration of multiple inflammatory factors, such as CRP/IL-1ß/IL-6 biomarker cluster in gestational 27-34 weeks, and the tumor necrosis factor/nerve growth factor (TNF/NGF) family during gestational 25-33 weeks, were potential biomarker clusters that specific for infectious PTB prediction.This study systematically pointed out prioritization of the inflammatory factors for infectious PTB prediction. The results also provided evidence that maternal inflammatory clusters can predict infectious PTB occurrence at accurate gestational week. The global consideration of multiple inflammatory factors at accurate gestational age is highlighted.

Orally administered octacosanol improves liver insulin resistance in high-fat diet-fed mice through the reconstruction of the gut microbiota structure and inhibition of the TLR4/NF-κB inflammatory pathway.

1-Octacosanol (Octa) is reported to possess many physiological properties. However, its relative mechanism has not been illustrated yet. Herein, we aimed to investigate the effect of Octa on insulin resistance in mice fed with a high fat diet (HFD) and used an in vitro simulated gastrointestinal tract to analyze its digestive behavior. The effects of Octa on the gut microbiota were verified by in vitro fermentation using the mouse fecal microbiota. As a result, the Octa monomer was digested into shortened saturated and unsaturated fatty acids (C10-C24) in the simulated gastrointestinal tract. Octa improved the fasting blood glucose (FBG), insulin resistance (IR), plasma lipids, and inflammatory response in HFD-fed mice in a dose-dependent manner. This study also suggested that a high-dose of Octa effectively decreased the levels of toll-like receptor 4 (TLR4), nuclear factor kappa-B (NF-κB), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) in the plasma of HFD-fed mice. Octa improved the oxidative stress induced by a HFD and increased the expression of the Nrf2/ARE signaling pathway. Importantly, Octa reshaped gut microbiota through decreasing Firmicutes content and increasing Bacteroidota and Verrucomicrobiota contents at the phylum level, and the changes of intestinal flora structure caused by Octa were significantly correlated with the changes of inflammatory biomarkers. In conclusion, the effects of Octa on insulin resistance might be attributed to the reconstruction of the gut microbiota structure and inhibition of the TLR4/NF-κB inflammatory pathway in HFD-induced obese individuals.

Nearly Monodisperse Copper Selenide Nanoparticles for Recognition, Enrichment, and Sensing of Mercury Ions.

In the current work, Cu(I)1.28Cu(II)0.36Se nanoparticles were synthesized via a simple procedure and were applied for the first time for recognition, adsorption, enrichment, and detection of Hg(II) ions. The experimental results show that 99.9% Hg(II) could be adsorbed by Cu(I)1.28Cu(II)0.36Se nanoparticles within just 30 s, and the Hg(II) concentration could be lowered down to a super-low level of 0.01 ppb. Cu(I)1.28Cu(II)0.36Se nanoparticles also demonstrate high selectivity to Hg(II) and Ag(I) among nine representative metal ions. The enrichment experiments show that Hg(II) of ultratrace concentration could be enriched significantly by Cu(I)1.28Cu(II)0.36Se nanoparticles, and thus, the detection limit of Hg(II) based on inductively coupled plasma emission spectroscopy-mass spectrometry would be pushed down by 2 orders of magnitude. These outstanding features of Cu(I)1.28Cu(II)0.36Se nanoparticles could be well accounted for in terms of the solubility product principle and the high affinity between selenium and mercury. Cu(I)1.28Cu(II)0.36Se nanoparticles were also found to have peroxidase-like activity, which could be inhibited by Hg(II) but not by Ag(I). This unique characteristic coupled with the solubility product principle successfully allows recognition and detection of Hg(II) even in the presence of Ag(I), which has a similar pKsp to Hg(II). As a result, the qualitative and quantitative analyses of Hg(II) could be performed by the naked eye and UV-visible spectroscopy, respectively. The current results indicate that Cu(I)1.28Cu(II)0.36Se nanoparticles not only have great potential in various aspects of dealing with Hg(II) pollution but would also shed light on discovering new nanomaterials to address other heavy metal ions.

Portable Hg2+ Nanosensor with ppt Level Sensitivity Using Nanozyme as the Recognition Unit, Enrichment Carrier, and Signal Amplifier.

We report a portable and highly sensitive Hg2+ nanosensor, where the CuS nanozyme functions as an Hg2+ recognition unit, a Hg2+ enrichment/preconcentration carrier, and a signal amplifier/output unit. The as-designed enrichment-detection integration strategy is customizable and endows the sensor with both a wide detection range from 50 ppt to 400 ppb and a high sensitivity with a minimum detectable Hg2+ concentration of 50 ppt. In order to make the Hg2+ nanosensor portable and cost-effective, a commercial RGB sensor is employed here in conjunction with the Hg2+-dependent colorimetric reaction. More importantly, the as-developed Hg2+ nanosensor is feasible for analysis of real samples with satisfactory accuracy (deviation <10%) and reproducibility (recovery ∼82%). Thus, this portable Hg2+ nanosensor appears to be a viable solution to meet the actual needs of on-site and real-time mercury contamination analysis and may also pave the way to colorimetric nanosensors for other metal ions.

Effects of self-injurious thoughts and behaviors and sexual risk-taking behaviors through emotional control.

BACKGROUND: College students are at increased risk for self-injurious thoughts and behaviors (SITB) and sexual risk behaviors (SRB). Although students with a history of SITB appear to be more prone to SRB, the mechanisms linking these risk behaviors remain largely unexplored. Previous research points to emotional control (EC), defined as one's awareness and adaptability of emotions, as a potential mechanism explaining the relationship between SITB and SRB.1 METHODS: Data included 722 college students attending two different universities in the northeast and southeast regions of the United States. Multiple group structural equation models were fit to estimate the direct and indirect effects of history of SITB (suicidal ideation, attempt, and nonsuicidal self-injury) and EC on SRB jointly across men and women. RESULTS: Findings supported indirect relationships between SITB and SRB through dysregulated EC, with type of SITB and patterns of SRB differing between men and women. For women, history of suicidal ideation and nonsuicidal self-injury were indirectly related to increased SRB through dysregulated EC. For men, history of suicidal ideation showed an indirect relationship on SRB through EC. LIMITATIONS: Although this study employed random sampling, limitations include a cross-sectional design, which does not allow for causal inference, and reliance on self-report assessment data. CONCLUSIONS: College students with a history of SITB who experience dysregulated EC may be more likely to engage in risky sexual behavior. Clinicians working with college students should concomitantly consider suicide and self-injury with SRB and consider interventions to improve EC.