Direct pulp capping (DPC) is a conservative approach for preserving tooth vitality without requiring more invasive procedures by enhancing pulp healing and mineralized tissue barrier formation. We investigated the effectiveness of Platelet Rich Plasma (PRP) vs. Mineral Trioxide Aggregate (MTA) as a DPC agent. Forty-two teeth from three mongrel dogs were divided into two equal groups. After three months, the animals were sacrificed to evaluate teeth radiographically using cone-beam computerized tomography, histopathologically, and real-time PCR for dentin sialophosphoprotein (DSPP), matrix extracellular phosphoglycoprotein (MEPE), and nestin (NES) mRNA expression. Radiographically, hard tissue formation was evident in both groups without significant differences (p = 0.440). Histopathologic findings confirmed the dentin bridge formation in both groups; however, such mineralized tissues were homogenous without cellular inclusions in the PRP group, while was osteodentin type in the MTA group. There was no significant difference in dentin bridge thickness between the PRP-capped and MTA-capped teeth (p = 0.732). The PRP group had significantly higher DSPP, MEPE, and NES mRNA gene expression than the MTA group (p < 0.05). In conclusion, PRP enables mineralized tissue formation following DPC similar to MTA, and could generate better cellular dentinogenic responses and restore dentin with homogenous architecture than MTA, making PRP a promising alternative DPC agent.
Platelet-Rich Plasma , Pulp Capping and Pulpectomy Agents , Animals , Dogs , Calcium Compounds/pharmacology , Root Canal Therapy , Silicates/pharmacology , Oxides/pharmacology , Aluminum Compounds/pharmacology , Drug Combinations , RNA, Messenger , Dental Pulp
Nanocomposite biomaterials combine a biopolymeric matrix structure with nanoscale fillers. These bioactive and easily resorbable nanocomposites have been broadly divided into three groups, namely natural, synthetic or composite, based on the polymeric origin. Preparing such nanocomposite structures in the form of hydrogels can create a three-dimensional natural hydrophilic atmosphere pivotal for cell survival and new tissue formation. Thus, hydrogel-based cell distribution and drug administration have evolved as possible options for bone tissue engineering and regeneration. In this context, nanogels or nanohydrogels, created by cross-linking three-dimensional polymer networks, either physically or chemically, with high biocompatibility and mechanical properties were introduced as promising drug delivery systems. The present review highlights the potential of hydrogels and nanopolymers in the field of craniofacial tissue engineering and bone regeneration.
Biocompatible Materials , Tissue Engineering , Nanogels/chemistry , Biocompatible Materials/chemistry , Tissue Engineering/methods , Polymers/chemistry , Bone Regeneration , Hydrogels/chemistry
Temporomandibular joint osteoarthritis (TMJ-OA) is a serious disease, designated by severe joint pain and dysfunction. Limitations of current therapeutics have led to an increased interest in regenerative strategies. Recently, the non-surgical treatment of OA has seen increased use of biologic injectable therapies like mesenchymal stem cells (MSCs) and platelet-rich plasma (PRP). Although these biotherapies represent an admirable effort, more studies are necessary to determine their efficacy. Thus, the aim of this study was to assess the curative potential of a single intra-articular injection of bone marrow MSCs-derived microvesicles (BM-MSCs-MVs) versus a single intra-articular injection of PRP in monoiodoacetate (MIA)-induced TMJ-OA model in Albino rats. Forty-eight male rats were used. A single intra-articular unilateral MIA injection was utilized to induce TMJ-OA. One week post induction, rats were sorted into 3 groups (16 rats each): group (I): received no treatment, groups (II) & (III): received BM-MSCs-MVs and PRP respectively. Scarification was done at 2 and 4 weeks from onset of treatment. Histological changes of the condylar TMJ were examined with H&E staining. Expression of IL-1ß, TNF-α, NF-κB, MMP-13, MMP-3, and collagen ΙΙ markers was detected using real-time PCR. Histologically, the osteoarthritic group exhibited degenerated condylar tissues which were aggravated at 4 weeks. Oppositely, a marked improvement in the condylar TMJ histology was noticed in both the BM-MSCs-MVs-and PRP-treated groups at both time intervals. Additionally, the treated groups showed a decrease in IL-1ß, TNF-α, NF-κB, MMP-13 and MMP-3 and an increase in collagen ΙΙ genes expression in contrast to the untreated group. Moreover, this difference was significant in the BM-MSCs-MVs group as compared to the PRP-treated group. Our results concluded that BM-MSCs-MVs as well as PRP treatments were able to target the key pathological features in OA, mainly inflammation and matrix degradation, and helped in restoring condylar structure in TMJ-OA rat model. However, BM-MSCs-MVs treatment exhibited more efficient therapeutic potential as compared to PRP treatment.
Diabetes mellitus is a challenging health problem. Salivary gland dysfunction is one of its complications. Current treatments possess numerous adverse effects. Therefore, herbal extracts have emerged as a promising approach for safe and effective treatment. However, they are required in large doses to achieve the desired effect. Accordingly, Origanum majorana extract (OE) was incorporated into nano-sized systems to enhance its biological effects at lower dosages. OE was standardized against rosmarinic acid (RA) and then loaded into nano-cubosomal (NC) systems via a 23 full-factorial design. Two optimum nano-systems at different drug loads (2.08 or 1.04 mg-RA/mL) were selected and assessed in vivo to compare their effects in streptozotocin-induced diabetic rats against conventional OE (2.08 mg-RA/mL). Blood glucose was evaluated weekly. Submandibular salivary glands were processed for histopathological examination and nuclear factor-erythroid 2-related factor 2 (Nrf2), Kelch-like ECH-associated protein 1 (Keap1), and p38-MAPK gene expression analysis. NC systems were successfully prepared and optimized where the optimum systems showed nano-sized vesicles (210.4-368.3 nm) and high zeta potential values. In vivo results showed a significant lower blood glucose in all treated groups, with an exceptional reduction with NC formulations. Marked histopathological improvement was observed in all OE-treated groups, with OE-NC4 (2.08 mg-RA/mL) demonstrating the best features. This was supported by RT-PCR; where the OE-NC4 group recorded the highest mean value of Nrf2 and the least mean values of Keap1 and p38-MAPK, followed by OE-NC3 and OE groups. In conclusion, OE-loaded NC enhanced the anti-hyperglycemic effect of OE and ameliorated diabetic gland alterations compared to conventional OE. Thus, cubosomal nano-systems could be anticipated as potential carriers for the best outcome with OE.
Diabetes Mellitus, Experimental/drug therapy , Origanum , Plant Extracts/pharmacology , Submandibular Gland/drug effects , Animals , Blood Glucose/drug effects , Chemistry, Pharmaceutical , Drug Carriers , Kelch-Like ECH-Associated Protein 1/genetics , Male , NF-E2-Related Factor 2/genetics , Nanostructures , Particle Size , Random Allocation , Rats , Streptozocin/pharmacology , Surface Properties , p38 Mitogen-Activated Protein Kinases/genetics
BACKGROUND: Enamel demineralisation is an initial step of the serious dental problem including dental caries, white spot lesions and dental erosion. AIM: Compare the effect of Er, Cr: YSGG (λ = 2780 nm) and nanosecond Nd: YAG (λ = 1064 nm) laser on enamel acid resistance. MATERIAL AND METHODS: Thirty non-carious human premolars, extracted for orthodontic reasons, were used. The experimental groups (n = 10 each group) were: Group I, untreated (control); Group II, Er, Cr: YSGG laser irradiation (0.75 W, 20 Hz, 140 µs, 10 s); Group III, nanosecond pulsed Nd: YAG laser irradiation (0.8 W, 10 Hz, 7 ns, 10 s). Scanning electron microscope and Energy Dispersive X-ray Spectroscopy (EDX) were used to assess acquired enamel resistance to PH cycling. RESULTS: After subjecting the three experimental groups to PH cycling, scanning electron microscopic examination revealed irregular porous dissoluted enamel surface in group I. However, groups II and III demonstrated partially dissoluted enamel surface. EDX analysis demonstrated the lowest mean percentage decrease in calcium and phosphorus content in group II followed by group III, then the highest mean percentage decrease was observed in untreated group I. One-way ANOVA revealed significant differences (p < 0.0001) between the tested groups. CONCLUSIONS: Both Er, Cr: YSSG and nanosecond Nd: YAG laser irradiation were able to improve the acid resistance of enamel. However, enamel surface treated with Er, Cr: YSSG laser showed the lowest mean percentage decrease of calcium and phosphorus (highest acid resistance).
