Dynamic actin/septin network in megakaryocytes coordinates proplatelet elaboration.

Megakaryocytes (MK) undergo extensive cytoskeletal rearrangements as they give rise to platelets. While cortical microtubule sliding has been implicated in proplatelet formation, the role of the actin cytoskeleton in proplatelet elongation is less understood. It is assumed that actin filament reorganization is important for platelet generation given that mouse models with mutations in actin-associated proteins exhibit thrombocytopenia. However, due to the essential role of the actin network during MK development, a differential understanding of the contribution of the actin cytoskeleton on proplatelet release is lacking. Here, we reveal that inhibition of actin polymerization impairs the formation of elaborate proplatelets by hampering proplatelet extension and bead formation along the proplatelet shaft, which was mostly independent of changes in cortical microtubule sliding. We identify Cdc42 and its downstream effectors, septins, as critical regulators of intracellular actin dynamics in MK, inhibition of which, similarly to inhibition of actin polymerization, impairs proplatelet movement and beading. Super-resolution microscopy revealed a differential association of distinctive septins with the actin and microtubule cytoskeleton, respectively, which was disrupted upon septin inhibition and diminished intracellular filamentous actin dynamics. In vivo, septins, similarly to F-actin, were subject to changes in expression upon enforcing proplatelet formation through prior platelet depletion. In summary, we demonstrate that a Cdc42/septin axis is not only important for MK maturation and polarization, but is further required for intracellular actin dynamics during proplatelet formation.

A Validation Study on the Physiological Parameters Recorded by COVIDBEEP - An Indigenous Remote Health Monitoring System Designed for COVID-19 Care in India.

Background: COVID-19 pandemic has affected mankind globally. After the three waves since March 2020, the threat continues instilling fear in the minds. Vital parameter monitoring through remote health monitoring system (RHMS) becomes critical for effective disease management and manpower safety and confidence. In a low resource setting like India, a comprehensive, wearable, and remotely operable device that is economical was required to be introduced for COVID-19 care. Present study validated the remote health monitoring device named COVIDBEEP with gold standard equipment. Materials and Methods: Six parameters, namely heart rate, SpO2, respiratory rate, temperature, blood pressure, and ECG were acquired in the supine position using the devices. Result: Analysis was performed using Graph Pad Prism. Intraclass correlation coefficients were used to measure concurrent validity. Bland-Altman graphs were plotted to know the agreement for each vital parameter. Confidence limits were set at 95%. All the parameters recorded from the devices showed a significant correlation with an "r" value between 0.5 and 0.9 with P value between 0.001 and 0.0002. Bland-Altman plots showed a minimum bias of 0.033 for heart rate and maximum of 3.5 for systolic blood pressure and respiratory rate. Conclusion: The association between the parameters recorded by the devices strengthened as the time of collection of data increased. Agreement between the two methods in 95% confidence interval was also proven to be significant for the parameters. Therefore, the indigenously developed COVIDBEEP has shown good validity in comparison to standard monitoring device.

Mycobacterium indicus pranii therapy suppresses systemic dissemination of tumor cells in B16F10 murine model of melanoma.

Cancer associated morbidity is mostly attributed to the dissemination of tumor cells from their primary niche into the circulation known as "metastasis". Mycobacterium indicus pranii (MIP) an approved immunotherapeutic agent against lung cancer (NSCLC) has shown potent anti-tumor activity in prior studies. While evaluating anti-tumor activity of MIP in mouse model, MIP treated animals typically exhibited less metastatic lesions in their pulmonary compartment. To study the role of MIP in metastasis closely, B16F10 melanoma cells were implanted subcutaneously in the mice, and the dissemination of tumor cells from the solid tumor was evaluated over a period of time. When B16F10 melanoma cells were treated with MIP in vitro, downregulation of epithelial mesenchymal transition markers was observed in these cells, which in turn suppressed the invasion, migration and adhesion of tumor cells. Notably, MIP therapy was found to be effectively reducing the metastatic burden in murine model of melanoma. Molecular characterization of MIP treated tumor cells substantiated that MIP upregulates the PPARγ expression within the tumor cells, which attenuates the NFκB/p65 levels within the nucleus, resulting in the suppression of Mmp9 expression in tumor cells. Besides that, MIP also downregulated the surface expression of chemokine receptor CXCR4 in murine melanoma cells, where chromatin immunoprecipitation confirmed the impeded recruitment of p50 and c-Rel factors to the Cxcr4 promoter, resulting in its downregulation transcriptionally. Taken together, MIP suppressed the dissemination of tumor cells in vivo, by regulating the expression of MMP9 and CXCR4 on these cells.

Effectiveness of metronidazole gel and mobile telephone short-message service reminders on gingivitis in orthodontic patients.

OBJECTIVES: To compare the effectiveness of metronidazole gel and mobile telephone short-message service (SMS) reminders on gingivitis in patients undergoing fixed orthodontic treatment. MATERIALS AND METHODS: The trial was double blinded (patient and investigator), and only the clinical trial unit pharmacist was unblinded. Data were collected from patients undergoing fixed orthodontic treatment for at least 6 months. A total of 66 patients were randomly assigned to either 0.8% metronidazole gel (n = 22), SMS reminder and placebo gel (n = 22), or placebo (control) group only (n = 22). Gingival index (GI), bleeding index (BI), and orthodontic plaque index (OPI) were evaluated on several teeth at baseline (T0) and after 4 weeks (T1). Paired-sample t-tests were used to compare mean differences of indexes at T0 and T1 in the groups, and independent-sample t-tests were used to determine the effects of interventions compared with the controls. RESULTS: Data from 64 patients were analyzed; there were 2 dropouts. There were statistically significant (P < .05) reductions in GI, BI, and OPI scores from T0 to T1 for each intervention. However, there were no significant differences between each intervention and the control group. There were no adverse effects. CONCLUSIONS: The null hypothesis could not be rejected. There is no difference between interventions (application of 0.8% metronidazole gel and SMS reminder for reinforcing oral hygiene) in reducing gingival inflammation in orthodontic patients.

Current trends in craniofacial distraction: A literature review.

The techniques and procedures involved in craniofacial distraction are constantly evolving. The understanding of histological and biochemical response at the distraction site is now improved. The cascade of events in distraction osteogenesis (DO) differs significantly from the typical fracture healing, and a better knowledge about these events has helped us identify suitable candidates for DO, make appropriate modifications to the distraction protocols and minimize the risk of complications. Recent advances in the manufacturing techniques have also facilitated the availability of distractors of various shapes and designs, which are now changing the way different craniofacial defects are being treated. Small but rigid intraoral distractors now enable easy placement, are well tolerated by patients and allow for a long consolidation period. The introduction of newer approaches toward treatment, together with the simultaneous management of different craniofacial defects at multiple osteotomy sites and enhanced surgical accuracy with the help of digital imaging, have made treatment outcomes more predictable.

Effectiveness of antimicrobial gels on gingivitis during fixed orthodontic treatment: A systematic review and meta-analysis.

OBJECTIVE: The present study was aimed to assess the effectiveness of antimicrobial gels along with conventional tooth brushing to improve gingival health in patients undergoing fixed orthodontic treatment. MATERIALS AND METHODS: All randomized and non-randomized clinical trials done on human subjects were explored in major health science databases (PubMed, CINAHL Plus, EBSCO Dent & Oral Sciences and Cochrane). An additional manual search was done on Google Scholar and on www.clinicaltrials.gov to identify any grey literature and unpublished data. Date of publication was not restricted during the data search. The assessment of risk of bias was done using the Cochrane Collaboration's Risk of Bias assessment tool. The meta-analysis was done using Review Manager Version 5.3.5 to analyse probing depth to be in two and four-week follow-up. This systematic review is reported according to the PRISMA statement and registered at PROSPERO (CRD42018084530). RESULTS: The electronic database search yielded 3733 records; hand search identified 14 articles meeting the selection criteria which were included in the qualitative data synthesis. Significant improvement in gingivitis has been reported using antioxidant-essential oil gel, cervitec gel (0.2% chlorhexidine), 2% chlorhexidine gel, amine fluoride gel, and 0.4% stannous fluoride gel with>98% availability of Sn+2 ions. Three articles with probing depth as comparable parameter were used for quantitative analysis. At the two and four-week follow-up, overall insignificant differences were observed in the antimicrobial gel group compared to the control group with regard to probing depth. CONCLUSIONS: The use of antioxidant-essential oil gel, amine fluoride gel, 0.4% stannous fluoride gel (98% availability of Sn+2) and 2% chlorhexidine gel resulted in significant improvement in gingivitis. However, probing depth in follow-up visits showed no significant difference between antimicrobial gel and control group.

The assessment of resting tongue posture in different sagittal skeletal patterns.

INTRODUCTION: Resting tongue posture affects the surrounding structures and, theoretically, may result in altered arch form and jaw relationship. OBJECTIVE: The objective of the present study was to investigate the association between resting tongue posture as observed in lateral cephalometric radiograph, sagittal jaw relationship and arch form. METHODS: The study was conducted on pretreatment lateral cephalograms and dental casts of 90 subjects. Subjects were equally divided into three groups, based on sagittal jaw relationship (Class I, II and III). Tongue posture was determined in terms of tongue-to-palate distances at six different points (distances 1 to 6) using ViewPro-X software, according to the method described by Graber et al in 1997. The arch widths (intercanine and intermolar widths) were evaluated on pretreatment dental casts. RESULTS: Tongue-to-palate distances were found to be comparable among different study groups. Significant differences were found in intercanine and intermolar widths at the cuspal and gingival levels among the study groups, except for intercanine width at cuspal level in maxilla and intermolar width at cuspal level in mandible. Moderate positive correlation was found between arch widths ratios at distances 3 and 4 in skeletal Class III group. Effect size was found to be moderate to large in different sagittal skeletal patterns and arch widths. CONCLUSION: The results of the current study showed no significant differences in the resting tongue posture among the groups, and moderate to weak correlation between tongue posture and dental arch widths.

The Association between Morphological Characteristics of Palatal Rugae and Sagittal Skeletal Patterns.

INTRODUCTION: To find the association between the morphological characteristics of palatal rugae and sagittal skeletal patterns. METHODS: The retrospective charts review was conducted at the Aga Khan University Hospital from April to June 2016, and comprised data obtained from the pretreatment dental cast of orthodontic patients visiting the dental clinics over 5 years from 2011 to 2015. The sample was divided equally in three malocclusion groups based on sagittal skeletal relationship Class I, Class II and Class III. The number of palatal rugae was recorded for both right and left sides. Morphological features of the three anterior most primary rugae were recorded for the length, pattern and orientation bilaterally. SPSS 20 was used to compare the number and lengths as well as the pattern and orientation of palatal rugae among the groups. RESULTS: Of the 165 subjects, there were 55(33.3%) in each of the three groups. Significant differences were recorded in rugae lengths among the groups at ruga 1 on both sides and rugae 2 and 3 on the left side (p<0.05 each). There were significant differences in the palatal rugae patterns among the groups (p<0.05) except at rugae 2 and 3 on the left side (p>0.05). Orientation showed significant differences at ruga 2 on the right side (p=0.02). CONCLUSIONS: No specific pattern was associated with any skeletal pattern. Further studies are required to evaluate three-dimensional characteristics of rugae to assess the association between palatal rugae and sagittal skeletal patterns.

The assessment of resting tongue posture in different sagittal skeletal patterns

ABSTRACT Introduction: Resting tongue posture affects the surrounding structures and, theoretically, may result in altered arch form and jaw relationship. Objective: The objective of the present study was to investigate the association between resting tongue posture as observed in lateral cephalometric radiograph, sagittal jaw relationship and arch form. Methods: The study was conducted on pretreatment lateral cephalograms and dental casts of 90 subjects. Subjects were equally divided into three groups, based on sagittal jaw relationship (Class I, II and III). Tongue posture was determined in terms of tongue-to-palate distances at six different points (distances 1 to 6) using ViewPro-X software, according to the method described by Graber et al in 1997. The arch widths (intercanine and intermolar widths) were evaluated on pretreatment dental casts. Results: Tongue-to-palate distances were found to be comparable among different study groups. Significant differences were found in intercanine and intermolar widths at the cuspal and gingival levels among the study groups, except for intercanine width at cuspal level in maxilla and intermolar width at cuspal level in mandible. Moderate positive correlation was found between arch widths ratios at distances 3 and 4 in skeletal Class III group. Effect size was found to be moderate to large in different sagittal skeletal patterns and arch widths. Conclusion: The results of the current study showed no significant differences in the resting tongue posture among the groups, and moderate to weak correlation between tongue posture and dental arch widths.

RESUMO Introdução: a postura da língua em repouso pode afetar as estruturas adjacentes e resultar em mudanças no formato das arcadas e na relação maxilomandibular. Objetivo: o presente estudo teve como objetivo avaliar a associação entre a postura da língua em repouso (observada em radiografias cefalométricas laterais), o padrão esquelético e o formato da arcada. Métodos: a amostra incluiu radiografias cefalométricas laterais e modelos de estudos iniciais de 90 pacientes, os quais foram divididos em três grupos, conforme a relação esquelética: Classe I, Classe II e Classe III. A postura da língua foi avaliada com o software ViewPro-X, de acordo com o método descrito por Graber et al. em 1997, medindo-se a distância entre o palato e a língua em seis pontos diferentes (distâncias 1 a 6). A largura das arcadas (distâncias intercaninos e intermolares) foi medida nos modelos de gesso iniciais. Resultados: as distâncias aferidas foram semelhantes entre os grupos. Diferenças significativas entre os grupos foram encontradas para as distâncias intercaninos e intermolares, nos níveis das cúspides e da gengiva, com exceção da distância intercaninos superiores ao nível das cúspides e distância intermolares inferiores ao nível das cúspides. No grupo Classe III, foi observada uma correlação positiva moderada entre as proporções das larguras das arcadas nas distâncias 3 e 4. O tamanho do efeito apresentou-se de moderado a alto nos diferentes padrões esqueléticos e larguras das arcadas. Conclusão: os resultados do presente estudo mostraram ausência de diferenças significativas na postura da língua em repouso entre os diferentes grupos, e uma correlação moderada a fraca entre a postura da língua e o formato das arcadas.

Adjunctive surgical procedures enhancing treatment outcome - A literature review.

The face is the most expressive zone of the human body that communicates our feelings and thoughts. This may also influence the interaction between people. The aesthetic adjunctive procedures are life-changing. In contemporary orthodontic treatment, orthognathic surgeries are performed to correct the functional aspects of dento facial deformities. In cases where the aesthetic outcome is not improved, patient dissatisfaction is often encountered. Many adjunctive surgical procedures can be used to enhance the anaesthetics of orthodontic or orthognathic surgical cases. Dwelling not merely on the ideal occlusion, the results could be enhanced by analysing the whole-face to improve the overall treatment outcome.

The association between palatal rugae pattern and dental malocclusion.

INTRODUCTION: Palatal rugae complete its development during early intrauterine life, whereas dental malocclusions in permanent dentition establishes several years into the post-natal life. OBJECTIVE: The objective of present study was to determine if there is an association between the palatal rugae pattern and Angle's classes of malocclusion. METHODS: A cross-sectional study was conducted on pretreatment dental casts of 184 patients. The sample was divided into the following groups: Class I, Class II div. 1, Class II div. 2, and Class III. The number of palatal rugae was recorded, bilaterally. The length, pattern and orientation of three anterior-most primary rugae on both sides were recorded. RESULTS: The mean age of the study sample was 17.8 ± 5.4 years. The mean number of the palatal rugae was 11.18 ± 2.5, with significant differences among different malocclusion groups. The length of the first rugae on left side and third rugae on both sides varied significantly among the groups (p< 0.05). Similarly, the pattern of palatal rugae was also found to be significantly different among the malocclusion groups. The right sided rugae did not have any significant difference in the orientation in different malocclusion groups; however, the left sided rugae showed significant differences among the four malocclusion groups (p< 0.001). CONCLUSIONS: The current study showed subtle differences in the palatal rugae pattern among the Angle's classes of malocclusion. Similarly, the length and orientation of some rugae were also found to be significantly different between malocclusion groups.

The association between palatal rugae pattern and dental malocclusion

ABSTRACT Introduction: Palatal rugae complete its development during early intrauterine life, whereas dental malocclusions in permanent dentition establishes several years into the post-natal life. Objective: The objective of present study was to determine if there is an association between the palatal rugae pattern and Angle's classes of malocclusion. Methods: A cross-sectional study was conducted on pretreatment dental casts of 184 patients. The sample was divided into the following groups: Class I, Class II div. 1, Class II div. 2, and Class III. The number of palatal rugae was recorded, bilaterally. The length, pattern and orientation of three anterior-most primary rugae on both sides were recorded. Results: The mean age of the study sample was 17.8 ± 5.4 years. The mean number of the palatal rugae was 11.18 ± 2.5, with significant differences among different malocclusion groups. The length of the first rugae on left side and third rugae on both sides varied significantly among the groups (p< 0.05). Similarly, the pattern of palatal rugae was also found to be significantly different among the malocclusion groups. The right sided rugae did not have any significant difference in the orientation in different malocclusion groups; however, the left sided rugae showed significant differences among the four malocclusion groups (p< 0.001). Conclusions: The current study showed subtle differences in the palatal rugae pattern among the Angle's classes of malocclusion. Similarly, the length and orientation of some rugae were also found to be significantly different between malocclusion groups.

RESUMO Introdução: as rugas palatinas completam seu desenvolvimento ainda no início da vida intrauterina, enquanto as más oclusões dentárias na dentição permanente se estabelecem após vários anos de vida pós-natal. Objetivos: o presente estudo foi planejado para determinar se há associação entre o padrão das rugas palatinas e as más oclusões, segundo a classificação Angle. Métodos: um estudo transversal foi conduzido a partir dos modelos dentários pré-tratamento de 184 pacientes. A amostra foi dividida em quatro grupos: Classe I; Classe II, divisão 1; Classe II, divisão 2 e Classe III. O número de rugas palatinas foi registrado bilateralmente. O comprimento, padrão e a orientação das três rugas primárias mais anteriores de ambos os lados foram registrados. Resultados: a idade média da amostra desse estudo foi de 17,8 ± 5,4 anos. O número médio de rugas palatinas foi de 11,18 ± 2,5, com diferenças significativas entre os grupos de más oclusões. O comprimento da primeira ruga do lado esquerdo e da terceira ruga em ambos os lados variou significativamente entre os grupos (p< 0,05). De forma semelhante, o padrão das rugas palatinas também mostrou diferença significativa entre os grupos com diferentes má oclusões. As rugas do lado direito não apresentaram nenhuma diferença significativa de orientação nos diferentes grupos; no entanto, as rugas do lado esquerdo apresentaram diferenças significativas nos grupos estudados (p< 0,001). Conclusões: o presente estudo demonstrou diferenças sutis quanto ao padrão das rugas palatinas entre as classes de má oclusão definidas por Angle. Do mesmo modo, o comprimento e a orientação de algumas rugas também se apresentaram consideravelmente diferentes entre os grupos com diferentes má oclusões.

Concepts, protocol, variations and current trends in surgery first orthognathic approach: a literature review.

In the current era of expedited orthodontics, among many clinicians, tertiary care hospitals and patients, surgery first orthognathic approach (SFOA) has gained popularity. The advantages of SFOA (face first approach) are the reduced overall treatment duration and the early improvement in facial esthetics. In SFOA, the absence of a presurgical phase allows surgery to be performed first, followed by comprehensive orthodontic treatment to achieve the desired occlusion. The basic concepts of surgery early, surgery last, SFOA and Sendai SFOA technique along with its variations are reviewed in the present article. The recent advancement in SFOA in the context of preoperative preparation, surgical procedures and post-surgical orthodontics with pertinent literature survey are also discussed.

Concepts, protocol, variations and current trends in surgery first orthognathic approach: a literature review

ABSTRACT In the current era of expedited orthodontics, among many clinicians, tertiary care hospitals and patients, surgery first orthognathic approach (SFOA) has gained popularity. The advantages of SFOA (face first approach) are the reduced overall treatment duration and the early improvement in facial esthetics. In SFOA, the absence of a presurgical phase allows surgery to be performed first, followed by comprehensive orthodontic treatment to achieve the desired occlusion. The basic concepts of surgery early, surgery last, SFOA and Sendai SFOA technique along with its variations are reviewed in the present article. The recent advancement in SFOA in the context of preoperative preparation, surgical procedures and post-surgical orthodontics with pertinent literature survey are also discussed.

RESUMO Na presente era da Ortodontia de resultados acelerados, a cirurgia ortognática com benefício antecipado (COBA) ganhou popularidade entre muitos clínicos, hospitais terciários e pacientes. A vantagem da COBA é a redução da duração total do tratamento, juntamente com a melhora precoce da estética facial. Na COBA, a ausência de uma fase pré-cirúrgica permite que a cirurgia seja realizada antes e, só então, venha o tratamento ortodôntico abrangente para se alcançar a oclusão desejada. Os conceitos básicos de cirurgia primeiro, cirurgia por último, COBA e a técnica COBA de Sendai, bem como suas variações, são aqui revistos. Também são discutidos no presente artigo, junto com a revisão da literatura pertinente, os recentes avanços da COBA no contexto do preparo pré-cirúrgico, dos procedimentos cirúrgicos e da Ortodontia pós-cirúrgica.

Reliability of overbite depth indicator (ODI) and anteroposterior dysplasia indicator (APDI) in the assessment of different vertical and sagittal dental malocclusions: a receiver operating characteristic (ROC) analysis.

INTRODUCTION:: Differential diagnosis of skeletal and dental relationships is crucial for planning orthodontic treatment. Overbite depth indicator (ODI) and anteroposterior dysplasia indicator (APDI) had been introduced in the past for assessment of vertical and sagittal jaw relationships, respectively. OBJECTIVE:: The objectives of this study were to evaluate the reliability of ODI and APDI in overbite and Angle malocclusions, as well as assess their diagnostic reliability among males and females of different age groups. MATERIAL AND METHODS:: This study was conducted using pretreatment dental casts and lateral cephalograms of 90 subjects. For ODI, subjects were divided into three groups based on overbite (normal overbite, open bite and deep bite). Likewise, the same subjects were divided for APDI into three groups, based on Angle's malocclusion classification (dental Class I, II and III malocclusions). Mann-Whitney U test was applied for comparison of study parameters regarding sex and different age groups. The mean values of ODI and APDI were compared among study groups by means of Kruskal-Wallis and post-hoc Dunnet T3 tests. The receiver operating characteristic (ROC) curve was applied to test diagnostic reliability. RESULTS:: Insignificant differences were found for ODI and APDI angles, particularly in regards to sex and age. Significant intergroup differences were found in different overbite groups and Angle's classification for ODI and APDI, respectively (p < 0.001). ROC showed 91% and 88% constancy with dental pattern in ODI and APDI, respectively. CONCLUSIONS:: ODI can reliably differentiate deep bite versus normal overbite and deep bite versus open bite. APDI can reliably differentiate dental Class I, II and III malocclusions.

Reliability of overbite depth indicator (ODI) and anteroposterior dysplasia indicator (APDI) in the assessment of different vertical and sagittal dental malocclusions: a receiver operating characteristic (ROC) analysis

ABSTRACT Introduction: Differential diagnosis of skeletal and dental relationships is crucial for planning orthodontic treatment. Overbite depth indicator (ODI) and anteroposterior dysplasia indicator (APDI) had been introduced in the past for assessment of vertical and sagittal jaw relationships, respectively. Objective: The objectives of this study were to evaluate the reliability of ODI and APDI in overbite and Angle malocclusions, as well as assess their diagnostic reliability among males and females of different age groups. Material and Methods: This study was conducted using pretreatment dental casts and lateral cephalograms of 90 subjects. For ODI, subjects were divided into three groups based on overbite (normal overbite, open bite and deep bite). Likewise, the same subjects were divided for APDI into three groups, based on Angle's malocclusion classification (dental Class I, II and III malocclusions). Mann-Whitney U test was applied for comparison of study parameters regarding sex and different age groups. The mean values of ODI and APDI were compared among study groups by means of Kruskal-Wallis and post-hoc Dunnet T3 tests. The receiver operating characteristic (ROC) curve was applied to test diagnostic reliability. Results: Insignificant differences were found for ODI and APDI angles, particularly in regards to sex and age. Significant intergroup differences were found in different overbite groups and Angle's classification for ODI and APDI, respectively (p < 0.001). ROC showed 91% and 88% constancy with dental pattern in ODI and APDI, respectively. Conclusions: ODI can reliably differentiate deep bite versus normal overbite and deep bite versus open bite. APDI can reliably differentiate dental Class I, II and III malocclusions.

RESUMO Introdução: o diagnóstico diferencial das relações esqueléticas e dentárias é essencial para o plano de tratamento ortodôntico. O indicador de profundidade da sobremordida (ODI)) e o indicador de displasia anteroposterior (APDI) foram desenvolvidos, no passado, para avaliação das relações verticais e sagitais dos maxilares, respectivamente. Objetivo: o objetivo desse estudo foi avaliar a confiabilidade do ODI e do APDI em diferentes sobremordidas e más oclusões de Angle, bem como verificar sua confiabilidade diagnóstica em homens e mulheres de diferentes grupos etários. Métodos: esse estudo foi conduzido utilizando-se modelos de estudo e radiografias cefalométricas laterais pré-tratamento de 90 indivíduos. Para o ODI, os indivíduos foram divididos em três grupos, com base na sobremordida (sobremordida normal, mordida aberta, mordida profunda). Ainda, para avaliação do APDI, esses mesmos indivíduos foram divididos em três grupos baseados na classificação de Angle para as más oclusões (Classes I, II e III dentárias). O teste U de Mann-Whitney foi aplicado para comparar os parâmetros estudados, quanto ao sexo e diferentes grupos etários. Os valores médios do ODI e do APDI foram comparados entre os grupos estudados por meio dos testes de Kruskal-Wallis e post-hoc T3 de Dunnett. A curva ROC (receiver operating characteristic) foi aplicada para testar a confiabilidade do diagnóstico. Resultados: diferenças não significativas foram encontradas para os ângulos ODI e APDI, particularmente em relação ao sexo e à idade. Diferenças significativas foram encontradas entre os grupos com diferentes sobremordidas e diferentes más oclusões de Angle, para o ODI e para o APDI, respectivamente (p < 0,001). A ROC mostrou 91% e 88% de concordância com o padrão dentário, para o ODI e APDI, respectivamente. Conclusões: o ODI mostrou-se confiável para diferenciar entre a mordida profunda e a sobremordida normal, e entre a mordida profunda e a mordida aberta. O APDI é confiável para se diferenciar entre as más oclusões dentárias de Classes I, II e III.

A coprological survey for assessing intensity of parasitic infection in school children: Cross-sectional study.

BACKGROUND: Intestinal parasitic infections (IPIs) are endemic worldwide and have been the cause of illness and disease worldwide. OBJECTIVES: The study is aimed to estimate prevalence, intensity, and factors associated with IPIs among school children aged 6-12 years. MATERIALS AND METHODS: This cross-sectional survey was carried out from February to July 2010, in the diagnostic laboratory of Microbiology department. The study group was divided into four groups, namely, Group A; Group B; Group C; and Group D. A total of 1769 eligible children were enrolled for sampling from these schools. For each enrolled child in the study, a standard stool ova and parasite test with formol-ether concentration technique was done for the assessment of the outcome. RESULTS: Among 1224 participants, 714 (58.3%) were boys and 508 (41.5%) were girls. The overall prevalence of IPIs was estimated as 51.5%. Group A 84%, Group B 64.7%, Group C 62.4%, and Group D 39.3%. Single IPIs were 65.7%, among which 48% were helminthic and 19.3% were protozoan. Multiple IPIs were 34.2%, among which protozoan along with helminthic were 25.9%, polyhelminthic were 8.5% and polyprotozoan were 4.2%. Among the IPIs detected, overall prevalence of helminth was 75.9%, protozoan was 24.1%. Among the helminthes hookworm was highest (28%). Among the protozoan Entamoeba histolytica/dispar was highest (14.8%). CONCLUSIONS: The study confirmed that prevalence of IPIs is high as 51.5%. Overall prevalence shows an endemic situation. Therefore, it is recommended that local health sectors should make provision for regular examination of parasitosis and deworming.