RESUMEN
Tri-n-butyl phosphate (TnBP) is commonly used as flame retardant and rubber plasticizer, and has been widely detected in aquatic organisms and natural waters. However, the potential toxicity of TnBP in fish remains unclear. In the present study, silver carp (Hypophthalmichthys molitrix) larvae were treated with environmentally relevant concentrations (100 or 1000 ng/L) of TnBP for 60 d and then they were depurated in clean water for 15 d, and the accumulation and depuration of the chemical in six tissues of silver carp were measured. Furthermore, effects on growth were evaluated and potential molecular mechanisms were explored. Results indicated that TnBP could be rapidly accumulated and depurated in silver carp tissues. In addition, the bio-accumulation of TnBP displayed tissue-specificity, where intestine contained the greatest and vertebra had the smallest level of TnBP. Furthermore, exposure to environmentally relevant concentrations of TnBP led to time- and concentration-dependent growth inhibition of silver carp, even though TnBP was completely depurated in tissues. Mechanistic studies suggested that exposure to TnBP up- and down-regulated the expression of ghr and igf1 in liver, respectively, and increased GH contents in plasma of silver carp. TnBP exposure also up-regulated the expression of ugt1ab and dio2 in liver, as well as decreased T4 contents in plasma of silver carp. Our findings provide direct evidence of health hazards of TnBP to fish in natural waters, calling for more attention of environmental risks of TnBP in aquatic environment.
Asunto(s)
Carpas , Animales , Bioacumulación , Organofosfatos/metabolismo , Hígado/metabolismoRESUMEN
Dietary intake is a crucial pathway of organophosphate esters (OPEs) exposure for human. However, information about the exposure risk of OPEs via rice consumption is still largely unknown. In the present study, a total of 234 rice samples from 25 provinces or city of China were collected and the concentrations of 24 OPEs were determined. Sixteen OPEs were detected in these rice samples and each rice sample was contaminated with at least 5 OPEs, indicating a ubiquitous occurrence of OPEs in Chinese rice. Moreover, the concentrations of Σ16 OPEs ranged from 1.46 to 552.65 µg/kg dry weight (dw), with a mean value of 64.74 µg/kg dw. For the composition profile of OPEs, three Cl-OPEs, including tris(2-chloroethyl) phosphate (TCEP), tri(2-chloroisopropyl) phosphate (TCIPP) and tris(1,3-dichloro-2-propyl) phosphate (TDCIPP), accounted for the highest proportion of Σ16 OPEs. For the spatial distribution of OPEs, although obvious spatial variations were observed among the 25 provinces or city, no obvious variations were found among the six rice-cultivating regions of China. Additionally, estimated dietary intakes (EDI) values of the 16 OPEs for adults and children were 1105.24 and 1399.13 ng/kg bw/day, respectively, under the high intake scenario. The hazard indexes of the 10 OPEs were 0.108 and 0.137 for adults and children, respectively. The risk assessment results indicated that Chinese adults and children did not suffer significant adverse effects from OPEs via rice intake.
Asunto(s)
Retardadores de Llama , Oryza , Adulto , Niño , Humanos , China , Monitoreo del Ambiente/métodos , Ésteres , Retardadores de Llama/análisis , Organofosfatos , FosfatosRESUMEN
The androgenic gland (AG) is a male-specific endocrine organ that controls the primary and secondary sexual characteristics in male crustaceans. More evidence indicates that the insulin-like androgenic gland hormone gene (IAG) is the key male sexual differentiation factor, particularly the application of RNA interference (RNAi) technology on IAG. In this study, the full-length cDNA of IAG (termed PcIAG) was isolated from the red swamp crayfish, Procambarusclarkii. Tissue distribution analysis showed that in addition to its expression in the AG of male P. clarkii, PcIAG was widely expressed in female tissues and other male tissues. The PcIAG protein was detected in the reproductive and nervous systems of adult male P. clarkii. Additionally, RNAi results showed that the PcIAG expression could be silenced efficiently, and the male sperm maturation and release possibly present a transient adverse interference at lower doses (0.1 µg/g and 1 µg/g) of PcIAG-dsRNA (PcIAG double-stranded RNA). Dramatically, the expression level of PcIAG increased sharply shortly after the injection of higher doses (5 µg/g and 10 µg/g) of PcIAG-dsRNA, which might accelerate the maturation and release of sperm. Moreover, the expression of PcSxl (P. clarkii Sex-lethal) was detected by Quantitative Real-Time PCR (qPCR) after the injection of PcIAG-dsRNA to explore whether the PcIAG gene regulates the PcSxl gene, and we found that the PcIAG did not directly regulate PcSxl in P. clarkii. The study could help accelerate the progress of PcIAG functional research and provide a useful reference for the single-sex selective breeding of P. clarkii.